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Genogroup position of aquabirnavirus GC-1 isolated from rockfish Sebastes schiegeli in Korea
Joh, Seong-Joon,Lee, Youn-Jeong,Song, Chang-Sun,Kang, Shien-Young,Mo, In-Pil,Heo, Gang-Jun The Korean Society of Veterinary Science 2008 大韓獸醫學會誌 Vol.48 No.3
The cDNA of the aquabirnavirus, GC-1 isolated from rockfish Sebastes schlegeli in Korea, was synthesized using the reverse transcriptase-polymerase chain reaction. The nucleotide and deduced amino acid sequences were determined from cDNA of the VP2-NS-VP3 coding region of genome segment A. The nucleotide sequences of the segment A were 3,086 base pairs (bp) in length and contained large open reading frame (ORF) and terminal sequences. The large ORF was comprised of 2,916 bp nucleotides and composed of 972 deduced amino acid sequences. Pairwise comparisons were made with other aquabirnavirus sequences published previously. The study of genetic relationships between GC-1 and aquabirnaviruses in the large ORF and VP2 coding regions demonstrated that the GC-1 has the nearest genetic relationship with the marine birnaviruses (MABV strains), and the GC-1 and MABV strains can be clustered as the same genogroup. GC-1 can be included in MABV, which is the 7th genogroup of family Aquabirnaviridae.
A report of 21 unreported bacterial species in Korea, belonging to the Betaproteobacteria
Kim, Pil Soo,Cha, Chang-Jun,Cho, Jang-Cheon,Chun, Jongsik,Im, Wan-Taek,Jahng, Kwang Yeop,Jeon, Che Ok,Joh, Kiseong,Kim, Seung Bum,Seong, Chi Nam,Yoon, Jung-Hoon,Bae, Jin-Woo The National Institute of Biological Resources 2016 Journal of species research Vol.5 No.1
As a subset investigation to discover indigenous prokaryotic species in Korea, a total of 21 bacterial strains assigned to the class Betaproteobacteria were isolated from a wide range of environmental samples which collected from fresh water, roots of plants, mineral water and soil from ginseng farm. Phylogenetic analysis based on 16S rRNA gene sequences indicated that 21 isolated strains were most closely related to the class Betaproteobacteria, with high 16S rRNA gene sequence similarity (>99.1%) and constructed a robust phylogenetic clade with the closest species in the class Betaproteobacteria. These isolated species have no previous report or publication in Korea; therefore 17 species in 14 genera of 6 families in the order Burkholderiales, 1 species in the order Methylophilales, 2 species in 2 genera of 1 family in the order Neisseriales are reported for betaproteobacterial species found in Korea. Gram reaction, colony and cell morphology, basic biochemical characteristics, isolation source, and strain IDs are also described in the species description section and as an image.
표피성장인자 연고의 국소도포가 당뇨 흰쥐의 피판 생존에 미치는 영향
임풍,조성필,손주영,안상태 大韓成形外科學會 1995 Archives of Plastic Surgery Vol.22 No.6
The topical application of epidermal growth factor (EGF) has been reported to be effective in the treatment of partial and full thickness skin wounds, second degree burns, incision wounds, and chronic ulcers. However, its effect on skin flap survival in diabetic patients has not yet been defined. The purpose of this experiment was to study whether EGF has a beneficial effect on the survival of distal skin flaps with impending necrosis in diabetic rats. 2×7cm sized skin flaps, which were insulated from their recipient bed, were made on the dorsum of 20 normal and 20 streptozotocin-induced diabetic rats. Half the animals in each group were treated with EGF ointment(10μg/g), and the other half were treated with ointment base only as control groups. Ointment was applied twice a day for five days. On the fifth postoperative day, the surviving flap area was measured using an image analysis system and histological changes were observed from the distal end of the surviving flap. The surviving areas(5.18±1.00cm2) of the diabetic control flaps were significantly decreased compared to those (7.83±1.67cm2) of the nomal control flaps. A significant enhancement of skin flap survival was observed in normal (8.42±0.14cm2) and diabetic (7.21±1.58cm2) rats after topical application of EGF. Flap survivial in EGF treated diabetic rats was approximately the same as that in normal control rats. Thick epidermis, prominent skin appendages, increased capillaries and fibroblasts, and linear organization of collagen fibers were obsered in EGF treated flaps.
조성돈,조성필,안상태,임풍 大韓成形外科學會 1998 Archives of Plastic Surgery Vol.25 No.2
The results of a retrospective study on 197 cases of malignant skin tumors, which were operated from July 1991 to June 1996 were presented. 1. Primary malignant skin tumors in our series included basal cell carcinoma(42%), squamous cell carcinoma(39%), melanoma(7%). Eighty five percentage of them were developed after 5th decade. There was a slight male preponderance (male to female ratio; 1.2:1). 2. seventy five percentage of malignant skin tumors were occurred in the head and neck. However 34% of squamous cell carcinoma occurred in the extremities. 3. Overall recurrence rate was 7.1% and overall metastasis rate was 7.6%. Seven cases(8.5%) of basal cell carcinoma were recurred without metastasis and seven cases(53.8%) of melanoma were meatastasized without recurrence. 4. Overall recurrence occurred at average 27 months after primary resection and 79% of them occurred in the head and neck. Among 14 recurred tumors, one recurred secondarily and three were metastasized elsewhere. 5.Two thirds of metastasis occurred within one year of resection. Mortality rate in the patients with metastasis was 40%. Metastases involved multiple organs and involved lymph nodes most frequently. 6.Fifteen patients with metastasis were treated with surgery alone(2 cases), surgery and adjuvant therapy(3 cases), radiation and/or chemolherapy without surgery(6 cases), and no therapy(4 cases).
임 풍,허 진,조성필,장익수,한기택 大韓成形外科學會 1996 Archives of Plastic Surgery Vol.23 No.3
Burn scar contractures of the various joint areas including anterior cervical region, popliteal region, ankle, antecubital region and wrist accompanied with severe functional limitations and esthetic problems. Many authors recommended full-thickness, split-thickness skin graft, local skin flap, or distant skin flap. But the recurrence of the contracture and many following procedures have been troublesome after these surgeries. Recent progress in the field of microvascular surgery has opened the way for various types of free transfer. The microvascular surgery enabled the various joint regions to be resurfaced with free flap and so the recurrence of contracture was prevented. Authors treated 20 patients with severe and recurrent burn scar contracture of the various joint regions using various microvascular free flap transfer. We obtained satisfactory functional results without late recurrence of the contracture of the transferred flaps.