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Seong, Y. J.,Park, H.,Yang, J.,Kim, S. J.,Choi, W.,Kim, K. H.,Park, Y. C. Springer Science + Business Media 2017 Applied microbiology and biotechnology Vol.101 No.9
<P>The SPT15 gene encodes a Saccharomyces cerevisiae TATA-binding protein, which is able to globally control the transcription levels of various metabolic and regulatory genes. In this study, a SPT15 gene mutant (S42N, S78R, S163P, and I212N) was expressed in S. cerevisiae BY4741 (BSPT15-M3), of which effects on fermentative yeast properties were evaluated in a series of culture types. By applying different nitrogen sources and air supply conditions in batch culture, organic nitrogen sources and microaerobic condition were decided to be more favorable for both cell growth and ethanol production of the BSPT15-M3 strain than the control S. cerevisiae BY4741 strain expressing the SPT15 gene (BSPT15wt). Microaerobic fed-batch cultures of BSPT15-M3 with glucose shock in the presence of high ethanol content resulted in a 9.5-13.4% higher glucose consumption rate and ethanol productivity than those for the BSPT15wt strain. In addition, BSPT15-M3 showed 4.5 and 3.9% increases in ethanol productivity from cassava hydrolysates and corn starch in simultaneous saccharification and fermentation processes, respectively. It was concluded that overexpression of the mutated SPT15 gene would be a potent strategy to develop robust S. cerevisiae strains with enhanced cell growth and ethanol production abilities.</P>
Seong, M.,Kim, S.,Yoo, H.,Choi, J. Elsevier Science Publishers 2016 Catalysis today Vol.260 No.-
<P>MnO2 was homogenously doped into anodic nanotubular TiO2 by a potential shock method, in which a high potential was imposed on the anodic nanotubes immediately after anodization process. We found that the potential shock process is inapplicable in a high-conductivity aqueous electrolyte, e.g. a MnO2 precursor solution (herein, 0.006 M KMnO4: 852-933 mu S/m). To avoid exceeding the output compliance of the current source, the potential shock voltage was optimized in ethylene glycol for the application of water oxidation. We found an optimal potential shock voltage of 140 V, which led to the doping of 0.7 at. % MnO2 into the high-aspect-ratio nanotubular TiO2 within 10 s. The TiO2 nanotubes doped with MnO2 were successfully employed as electrodes for the non-noble catalysis of water oxidation. Although the doping concentration of Mn was found to be linearly proportional to the applied potential shock voltages, potential shocks greater than 140 V significantly increased the thickness of the barrier oxide layer, which increased the overpotential in the water oxidation process. (C) 2015 Elsevier B.V. All rights reserved.</P>
Superconducting property of single-crystal like MgB<sub>2</sub> thin film
Seong, W.K.,Kang, W.N.,Oh, S.J.,Jung, J.K.,Kim, C.J.,Joo, J. North-Holland 2010 Physica. C, Superconductivity Vol.470 No.20
We have grown single-crystal like MgB<SUB>2</SUB> thin films on (0001) Al<SUB>2</SUB>O<SUB>3</SUB> substrates by hybrid physical-chemical vapor deposition technique. The microstructures of single-crystal quality of MgB<SUB>2</SUB> thin films were studied by a transmission electron microscopy and a selected area electron diffraction pattern (SAED). The SAED pattern of the cross section shows the phase pure in the MgB<SUB>2</SUB> thin film. The magnetic field dependence of the critical current density (J<SUB>c</SUB>) was reflected to be a very weak pinning behavior at high fields as similar to that of MgB<SUB>2</SUB> single-crystal.
Growth of Epitaxial MgB<sub>2</sub> Thick Films with Columnar Structures by Using HPCVD
Seong, W. K.,Huh, J. Y.,Kang, W. N.,Kim, J.-W.,Kwon, Y.-S.,Yang, N.-K.,Park, J.-G. Wiley - VCH 2007 CHEMICAL VAPOR DEPOSITION -WEINHEIM- Vol.13 No.12
<P>Epitaxial MgB<SUB>2</SUB> thick films are grown on Al<SUB>2</SUB>O<SUB>3</SUB> substrates at 600 °C by using the hybrid physical (HP)CVD technique. In order to obtain a high magnesium vapor pressure around the substrates, we use a special susceptor having a susceptor cap and achieve a very high growth rate of 0.17 &mgr;m min<SUP>–1</SUP>. Hexagonal-shaped columnar structures are observed by cross-sectional and planar-view transmission electron microscope (TEM) images. For the 1.7 &mgr;m thick film, the T<SUB>c</SUB> is observed to be 40.5 K with a J<SUB>c</SUB> of 1.5 × 10<SUP>6</SUP> A cm<SUP>–2</SUP> at 30 K. The vortex pinning mechanism by intercolumnar boundaries will be discussed.</P> <B>Graphic Abstract</B> <P>By using novel HPCVD technique with a special susceptor and a susceptor cap, epitaxial MgB<SUB>2</SUB> thick films with columnar structures are successfully fabricated at low temperatures (below 600 °C). The susceptor cap significantly enhances the local magnesium vapor pressure around the substrates so that a very high growth rate of 0.17 &mgr;m/min could be achieved. For the 1.7-&mgr;m-thick film, the T<SUB>c</SUB> was observed to be 40.5 K.</P>
Yoo, S.,Lee, J.,Kim, J.M.,Seong, C.Y.,Seong, K.d.,Piao, Y. Elsevier Sequoia 2016 Journal of Electroanalytical Chemistry Vol.780 No.-
<P>For large scale batteries, sulfur is an attractive cathode active material for lithium batteries because of high theoretical capacity of 1675 mA hg(-1). However, severe capacity fading and low conductivity of sulfur are significant challenges for its practical application. Here, we report a facile approach to prepare reduced graphene oxide nanoscroll embedded with well-dispersed sulfur (S/GNSC) through a one-pot oxidation of sulfide and reduction of graphene oxide followed by freeze-casting process to generate graphene nanoscroll. The S/GNSC takes the novel shape of reduced graphene oxide sheet spirally wrapped into one-dimensional tubular structure, which enhances electrochemical performance by acting as physical barrier to prevent polysulfide from dissolving and an effective network to promote electron and Li+ transport during reaction. Therefore, the S/GNSC composite with high sulfur loading of 76 wt% exhibits high initial capacity of 1295 mA h g(-1) and reversible capacity of 744 mA h g(-1) after the 100th cycle at 0.2 degrees C when it is used as a cathode material for lithium-sulfur battery, while the capacity of sulfur loaded on reduced graphene oxide sheet (S/rGO) is continuously fading to 510 mA h g(-1) after the 100th cycle due to the irreversible loss of polysulfide. (C) 2016 Elsevier B.V. All rights reserved.</P>
孟琦錫,洪元杓,任洪彬,李廷玟,朴萬植,李載元,金八圭,權五憲,成世鎭,朴東徹,金太均,李定根 충남대학교 공업교육연구소 1981 論文集 Vol.4 No.1
Since the College of Industrial Education of Chungnam National University took charge of technical teacher preparation in 1977, much efforts have been concentrated on the activities for creating favorable educational environments in terms of curricula, pedagogic affairs, inner and outer facilities, etc. In this study some imposed barriers which are likely to hinder this college from achieving better education were identified and analysed. Considerably large scale of the student enrollment in comparison with the market demand is a prevailing issue which causes an adverse effect for clarification of the educational goal of the college and another problem is concerned with the student entry criteria which do not require the pre-experiences in the field. Under this circumstances, a guide line to improve the teacher education is recommended as follow; 1) The number of the student enrollment should be adjusted to meet the minimum range in the wave of the technical teacher demand projection and the shortage of teacher could be covered by the graduates from the shortterm courses in this college. 2) The current curriculum is accordingly recommended to be diversified taking into consideration the qualification of the student. The suggested consists of four-year regular course, one-year course, and six-month course and the short-term courses are provided to induce the experienced people from industries. 3) In order to have teachers adjust themselves to changing technology, education on sound and fundamental knowledges should be provided in the pre-service education course and education on advanced knowledges and applied theories deferred to the in-service training course. 4) In the four-year pre-service education, the general education and pedagogic subjects are to be drastically intensified from 18 credits and 22 credits up to 40 credits and 30 credits respectively.
Jo, S.E.,Seong, Y.J.,Lee, H.S.,Lee, S.M.,Kim, S.J.,Park, K.,Park, Y.C. Elsevier Science Publishers 2016 Journal of biotechnology Vol.227 No.-
<P>Xylose is a major monosugar in cellulosic biomass and should be utilized for cost-effective ethanol production. In this study, xylose-converting ability of recombinant Saccharomyces cerevisiae SX6MUT expressing NADH-preferring xylose reductase mutant (R276H) and other xylose-metabolic enzymes, and deficient in aldehyde dehydrogenase 6 (Ald6p) were characterized at microaerobic conditions using various sugar mixtures. The reduction of air supply from 0.5 vvm to 0.1 vvm increased specific ethanol production rate by 75% and did not affect specific xylose consumption rate. In batch fermentations using various concentrations of xylose (50-104 g/L), higher xylose concentration enhanced xylose consumption rate and ethanol productivity but reduced ethanol yield, owing to the accumulation of xylitol and glycerol from xylose. SX6MUT consumed monosugars in pitch pine hydrolysates and produced 23.1 g/L ethanol from 58.7 g/L sugars with 0.39 g/g ethanol yield, which was 14% higher than the host strain of S. cerevisiae D452-2 without the xylose assimilating enzymes. In conclusion, S. cerevisiae SX6(MUT) was characterized to possess high xylose-consuming ability in microaerobic conditions and a potential for ethanol production from cellulosic biomass. (C) 2016 Elsevier B.V. All rights reserved.</P>
Yun, S.M.,Woo, S.H.,Oh, S.T.,Hong, S.E.,Choe, T.B.,Ye, S.K.,Kim, E.K.,Seong, M.K.,Kim, H.A.,Noh, W.C.,Lee, J.K.,Jin, H.O.,Lee, Y.H.,Park, I.C. North-Holland 2016 Molecular and cellular endocrinology Vol.422 No.-
Melatonin is implicated in various physiological functions, including anticancer activity. However, the mechanism(s) of its anticancer activity is not well understood. In the present study, we investigated the combined effects of melatonin and arsenic trioxide (ATO) on cell death in human breast cancer cells. Melatonin enhanced the ATO-induced apoptotic cell death via changes in the protein levels of Survivin, Bcl-2, and Bax, thus affecting cytochrome c release from the mitochondria to the cytosol. Interestingly, we found that the cell death induced by co-treatment with melatonin and ATO was mediated by sustained upregulation of Redd1, which was associated with increased production of reactive oxygen species (ROS). Combined treatment with melatonin and ATO induced the phosphorylation of JNK and p38 MAP kinase downstream from Redd1 expression. Rapamycin and S6K1 siRNA enhanced, while activation of mTORC1 by transfection with TSC2 siRNA suppressed the cell death induced by melatonin and ATO treatment. Taken together, our findings suggest that melatonin enhances ATO-induced apoptotic cell death via sustained upregulation of Redd1 expression and inhibition of mTORC1 upstream of the activation of the p38/JNK pathways in human breast cancer cells.