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- 검색키워드
- 저자 : Schellenberg, Maja (검색결과 3 건)
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Schneider, Thomas,Schellenberg, Maja,Meyer, Stefan,Keller, Felix,Gehrig, Peter,Riedel, Kathrin,Lee, Youngsook,Eberl, Leo,Martinoia, Enrico WILEY-VCH Verlag 2009 Proteomics Vol.9 No.10
<P>Although the vacuole is the most important final store for toxic heavy metals like cadmium (Cd<SUP>2+</SUP>), our knowledge on how they are transported into the vacuole is still insufficient. It has been suggested that Cd<SUP>2+</SUP> can be transported as phytochelatin-Cd<SUP>2+</SUP> by an unknown ABC transporter or in exchange with protons by cation/proton exchanger (CAX) transporters. To unravel the contribution of vacuolar transporters to Cd<SUP>2+</SUP> detoxification, a quantitative proteomics approach was performed. Highly purified vacuoles were isolated from barley plants grown under minus, low (20 μM), and high (200 μM) Cd<SUP>2+ </SUP>conditions and protein levels of the obtained tonoplast samples were analyzed using isobaric tag for relative and absolute quantitation (iTRAQ™). Although 56 vacuolar transporter proteins were identified, only a few were differentially expressed. Under low-Cd<SUP>2+</SUP> conditions, an inorganic pyrophosphatase and a γ-tonoplast intrinsic protein (γ-TIP) were up-regulated, indicating changes in energization and water fluxes. In addition, the protein ratio of a CAX1a and a natural resistance-associated macrophage protein (NRAMP), responsible for vacuolar Fe<SUP>2+</SUP> export was increased. CAX1a might play a role in vacuolar Cd<SUP>2+</SUP> transport. An increase in NRAMP activity leads to a higher cytosolic Fe<SUP>2+</SUP> concentration, which may prevent the exchange of Fe<SUP>2+</SUP> by toxic Cd<SUP>2+</SUP>. Additionally, an ABC transporter homolog to AtMRP3 showed up-regulation. Under high Cd<SUP>2+</SUP> conditions, the plant response was more specific. Only a protein homologous to AtMRP3 that showed already a response under low Cd<SUP>2+</SUP> conditions, was up-regulated. Interestingly, AtMRP3 is able to partially rescue a Cd<SUP>2+</SUP>-sensitive yeast mutant. The identified transporters are good candidates for further investigation of their roles in Cd<SUP>2+</SUP> detoxification.</P>
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Chengyu Yang,Xuesong Cai,Gaojie Dong,Andreas Schellenberg,Shawn You 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.7
This paper recounts a real-time hybrid simulation (RTHS) that employed a shake table coupled with an actuator to evaluate the seismic performance of a single-span girder bridge. The test specimen for this RTHS was a scaled single-span girder bridge, which was subjected to Loma Prieta excitation in the test. In the RTHS, the bridge specimen, which lacked mass on top of the bridge deck, was physically tested by a shake table and an actuator, whilst the mass on the bridge, as well as the pile foundation and the soil were modeled numerically. OpenSEES was used for numerical modeling and OpenFresco was used for the communication between the test system and numerical model. An adaptive time series (ATS) technique was employed to compensate for time delay in the RTHS. In this way, a novel bridge engineering RTHS application case composed of shake table and actuator control was presented. To validate the results of the RTHS, a shake table test (STT) of the bridge structure with full mass on the bridge deck was conducted correspondingly. Comparing the RTHS and STT results, it can be concluded that acceleration histories, bearing deformation histories, and strain histories of the selected points matched well. The application of RTHS to assess the seismic behavior of the single-span girder bridge was reasonably verified in this paper.
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Efficiency Enhancement of mm-Wave Power Amplifiers Using Envelope Tracking
Yan, J J,Presti, C D,Kimball, D F,Young-Pyo Hong,Chin Hsia,Asbeck, P M,Schellenberg, J IEEE 2011 IEEE microwave and wireless components letters Vol.21 No.3
<P>This letter presents a 44 GHz GaAs MMIC-based power amplifier (PA) which uses envelope tracking (ET) techniques for efficiency enhancement. Digital pre-distortion (DPD) is also employed to achieve high linearity. For a 7.6 dB PAPR 64QAM 20 MHz bandwidth signal (data rate 120 Mb/s) at an output power of 23.8 dBm, the measured EVM was 2.0% and ACPR1 was better than -40 dBc. Power efficiency enhancement of more than 5.7 times for the final MMIC stage (from 1.22% to 7%) was measured, using an external drain modulator. To the authors' best knowledge, this is the first time envelope tracking has been applied to PAs in the millimeter wave regime.</P>
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