우라늄 투여후 간조직에서의 효소활성도의 변화

김국찬,이강석,김진규,김인규,박효국,천기정,김상복 대한방사선 방어학회 1993 방사선방어학회지 Vol.18 No.2

우라늄 피폭후 효소활성도가 내부피폭의 biomarker로서의 가능성을 연구하기 위하여 수서생물인 잉어9Caprinus carpio)의 복강내 우라늄 투여시 간조직에서 일어나는 여러가지 효소계의 변화를 조사하였다. 이러한 효소활성도의 변화는 우라늄 내부피폭의 biomarker로서 가능성을 가지고 있으며 연구결과는 다음과 같다. 1) 6일째까지 우라늄을 주사하여 적출한 간에서의 전체단백질량은 계속 감소하고 있다. 2) 세포내의 lysosome내에 함유하고 있는 acid protease와 β-glucuronidase의 활성도는 6일째 투여후까지 활성도가 감소하고 있다. 3) Alkaline phosphatase의 경우 6일째 우라늄 투여후까지 증가하고 있으며 반대로 acid phosphatase의 경우 6일째 우라늄 투여후까지 활성도가 급격히 증가하였고 glutamate oxaloacetate transaminase의 활성도는 완만하게 증가하고 있다. 4) Creatine kinase의 활성도는 완만한 감소를 보이고 있으며 malate dehydrogenase는 첫번 우라늄 투여후에 활성도가 급격히 감소하였고 3일째 우라늄 투여후에는 활성도가 거의 나타나지 않았다. We examined various enzyme activity changes by intraperitioneal injection uranium in the carp liver. These enzyme activity changes can be used as biochemical indicators of internal exposure to uranium. The results were followings ; 1) Total protein concentration decreased by intraperitoneal injection in the carp liver. 2) Lysosomal acid protease and β-glucronidase activities increased in the liver until sixth intraperitoneal injection of uranium, but lysosomal acid phosphatase activities decreased in the liver until the sixth injection of uranium. 3) Alkaline phosphatase activities sharply increased and Glutamate oxaloacetate Transaminase activities steadily decreased in the liver until the sixth injection of uranium. 4) Creatine %kinase activities steadily decreased and malate dehydrogenase activities sharply decreased in the liver after the primary injection of uranium. Any malate dehydrogenase activities was not detected after sixth injection of uranium.

흰쥐의 방사선 피폭후 생물학적 지표로서의 혈액효소

김진규,김상복,김국찬,천기정,김인규,박효국,이강석 대한방사선 방어학회 1993 방사선방어학회지 Vol.18 No.2

생물학적 선량평가를 위한 생화학적 지표 연구로서 흰쥐 혈액내 효소활성도의 변화를 조사한 결과는 다음과 같다. 1) Alkaline phosphatase 활성도는 0.1, 0.25, 0.5, 2, 4 Gy의 방사선 조사후 24시간까지 혈액내 활성도가 증가하였고 72시간 경과시에는 대조군과 비슷한 활성도를 보였다. Creatine kinase는 2, 4 Gy 방사선 조사후 혈액내에서 활성도에서 활성도가 72시간까지 증가하였으나 0.1, 0.25Gy, 0.5 Gy 방사선 조사시에는 커다란 변화를 보이지 않았다. 2) Malate dehydrogenase 활성도는 0.1, 0.25, 0.5 Gy 방사선 조사시에는 커다란 변화가 없었으며 lactate dehydrogenase는 방사선 조사후 활성도가 감소하였다. 3) GOT의 활성도는 선량률 0.1Gy/min.로 0.1, 0.25, 0.5, 2, 4 Gy 조사후에는 어떠한 변화도 없었으며 선량률 0.5 Gy/sec.로 0.5, 1, 1.5, 2, 3, 5, 7 Gy로 조사후에는 증가현상을 보이고 있다. Acid phosphatase 활성도는 상기의 어떠한 선량에서도 나타나지 않고 있다. 잠재적으로 이러한 효소들은 방사선 피폭의 지표물질로 사용될 수 있으며 생화학적 지식과 기술을 이용한 좀더 나은 지표물질을 찾기 위하여 계속적인 조사가 필요하다. Enzyme activity changes in rat blood as biochemical indicator useful for evaluating exposure dose were experimentally studied. The experimental results obtained are as follows: 1) Alkaline phosphatase activities increased in the blood serum until 24 hours after 0.1, 0.25, 0.5, 2 and 4 Gy irradiation and its activities returned mormal condition after 72 hours of post-irradiation. Creatine kinase activities increased in the blood serum until 72 hours after 2 and 4 Gy irradiation but any significant activity changes were not detected after 0.1, 0.25 Gy irradiation. 2) Malate dehydrogenase activities did not reveal available changes changes after 0.1, 0.25, 0.5 Gy irradiation and lactate dehydrogenase activities decreased in the blood serum after 0.1, 0.25, 0.5 Gy irradiation. 3) Glutamate oxaloacetate transaminase activity changes were detected in the blood serum after 0.1, 0.25, 0.5, 2, 4 Gy(0.1 Gy/min.) and GOT activities increased after 0.5, 1, 1.5, 2, 3, 5, 7 Gy(0.5 Gy/sec.). Any acid phosphatase activities were detected in the blood serum after 0.1, 0.25, 0.5, 2, 4 Gy(0.1 Gy/min.) and 0.5, 1, 1.5, 2, 3, 5, 7 Gy(0.5 Gy/sec.) irradiation. Potentially some of these enzymes can be used as indicator protein for radiation injury. Futher investigation is needed to find better biochemical indicatiors utilizing recent knowledge and techniques of biochemistry.

Poster Session 2 / G-34 : The Role of Endothelial Monocyte - activating Polypeptide-2 ( EMAP-2 ) on mRNA Processing

( Sang Gyu Park ),( Sung Hoon Kim ),( Young Sun Kang ),( Young Kug Jang ),( Keum Hee Chung ) 한국생화학분자생물학회 (구 한국생화학회) 1999 생화학분자생물학회 춘계학술발표논문집 Vol.- No.-

Large-Scale Synthesis of Carbon-Shell-Coated FeP Nanoparticles for Robust Hydrogen Evolution Reaction Electrocatalyst

Chung, Dong Young,Jun, Samuel Woojoo,Yoon, Gabin,Kim, Hyunjoong,Yoo, Ji Mun,Lee, Kug-Seung,Kim, Taehyun,Shin, Heejong,Sinha, Arun Kumar,Kwon, Soon Gu,Kang, Kisuk,Hyeon, Taeghwan,Sung, Yung-Eun American Chemical Society 2017 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.139 No.19

<P>A highly active and stable non-Pt electrocatalyst for hydrogen production has been pursued for a long time as an inexpensive alternative to Pt-based catalysts. Herein, we report a simple and effective approach to prepare high-performance iron phosphide (FeP) nanopartide electrocatalysts using iron oxide nanoparticles as a precursor. A singlestep heating procedure of polydopamine-coated iron oxide nanoparticles leads to both carbonization of polydopamine coating to the carbon shell and phosphidation of iron oxide to FeP, simultaneously. Carbon-shell-coated FeP nanoparticles show a low overpotential of 71 mV at 10 mA cm(-2), which is comparable to that of a commercial Pt catalyst, and remarkable long-term durability under acidic conditions for up to 10 000 cycles with negligible activity loss. The effect of carbon shell protection was investigated both theoretically and experimentally. A density functional theory reveals that deterioration of catalytic activity of FeP is caused by surface oxidation. Extended X-ray absorption fine structure analysis combined with electrochemical test shows that carbon shell coating prevents FeP nanoparticles from oxidation, making them highly stable under hydrogen evolution reaction operation conditions. Furthermore, we demonstrate that our synthetic method is suitable for mass production, which is highly desirable for large-scale hydrogen production.</P>

LIQUID LENS BASED ON ELECTROMAGNETIC ACTUATION FOR HIGH-PERFORMANCE MINIATURE CAMERAS

Sang Hoon Oh(오상훈),Jeong Hwa Seo(서정화),Jin Pyo Jeon(전진표),Sang Kug Chung(정상국) (사)한국CDE학회 2015 한국 CAD/CAM 학회 학술발표회 논문집 Vol.2015 No.동계

Microbiological and Chemical Detection of Antibiotic Residues in Livestock and Seafood Products in the Korean Market

Sung-Kug Park,Meehye Kim,You Sub Sho,So Young Chung,Soojung Hu,Jong Ok Lee,Moo Ki Hong,Myung Chul Kim,Ju Seop Kang,Ok Hwa Jhee 한국식품과학회 2007 Food Science and Biotechnology Vol.16 No.6

The microbiological and chemical identification of antibiotic residues was attempted for livestock and seafood products including pork (n=34), beef (n=34), chicken (n=32), flatfish (n=37), armorclad rockfish (n=36), and sea bream (n=27). The meat (n=100) and seafood (n=100) samples were collected from 9 markets in 5 major Korean cities. Antibiotic substances were identified from the classes of tetracyclines, macrolides, penicillins, aminoglycosides, polyethers, peptides, sulfonamides, quinolones, chlorampenicols, and novobiocins using a microbiological assay, the Charm II test and high performance liquid chromatography (HPLC) with ultra violet (UV) and fluorescence detectors. The results showed that 2 tetracyclines (oxytetracycline and tetracycline) and 3 quinolones (ciprofloxacin, norfloxacin, and enrofloxacin) were detected in 4 samples of flatfish among all 100 seafood samples tested. No antibiotic residues were detected in the 100 livestock product samples tested. The amounts (min-max, mg/kg) of the residual antibiotics were as follows; tetracycline 0.78-0.85, oxytetracycline 0.49-0.74, ciprofloxacin 0.09-0.83, norfloxacin 0.01-0.21, enrofloxacin 0.12-2.98. These data indicate that the total detection rate of antibiotics in livestock and seafood products was approximately 2%.

Enhancement of the response time of EWOD actuation using overdrive voltage

Sung Jin Hong(홍성진),Jeong Byung Chae(채정병),HeeWon Seo(서희원),Sang Kug Chung(정상국) (사)한국CDE학회 2015 한국 CAD/CAM 학회 학술발표회 논문집 Vol.2015 No.동계

Constructing Space of Shared Value

Sung-Cheol Lee,Young-kug Joh,Su-Yuel Chung 대한지리학회 2014 대한지리학회 학술대회논문집 Vol.2014 No.6

Fast Electrically Driven Capillary Rise Using Overdrive Voltage

Hong, Sung Jin,Hong, Jiwoo,Seo, Hee Won,Lee, Sang Joon,Chung, Sang Kug American Chemical Society 2015 Langmuir Vol.31 No.51

<P>Enhancement of response speed (or reduction of response time) is crucial for the commercialization of devices based on electrowetting (EW), such as liquid lenses and reflective displays, and presents one of the main challenges in EW research studies. We demonstrate here that an overdrive EW actuation gives rise to a faster rise of a liquid column between parallel electrodes, compared to a DC EW actuation. Here, DC actuation is actually a simple applied step function, and overdrive is an applied step followed by reduction to a lower voltage. Transient behaviors and response time (i.e., the time required to reach the equilibrium height) of the rising liquid column are explored under different DC and overdrive EW actuations. When the liquid column rises up to a target height by means of an overdrive EW, the response time is reduced to as low as 1/6 of the response time using DC EW. We develop a theoretical model to simulate the EW-driven capillary rise by combining the kinetic equation of capillary flow (i.e., Lucas–Washburn equation) and the dynamic contact angle model considering contact line friction, contact angle hysteresis, contact angle saturation, and the EW effect. This theoretical model accurately predicts the outcome to within a ± 5% error in regard to the rising behaviors of the liquid column with a low viscosity, under both DC EW and overdrive actuation conditions, except for the early stage (<about 20 ms).</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/langd5/2015/langd5.2015.31.issue-51/acs.langmuir.5b02921/production/images/medium/la-2015-02921k_0008.gif'></P>

베트남 커피변경지역의 글로벌 가치사슬과 공유가치 창출

이승철 ( Sung-cheol Lee ),정수열 ( Su-yuel Chung ),조영국 ( Young-kug Joh ) 한국경제지리학회 2016 한국경제지리학회지 Vol.19 No.2

본 연구의 목적은 베트남 커피변경지역이 글로벌 가치사슬에 통합되는 과정에서 나타나는 가치의 전유 및 실현관계를 고찰하고, 공유가치창출에 대한 지속가능한 커피 글로벌 가치사슬의 의미를 분석하고자 한다. 최근 베트남의 대표적 커피 변경지역인 닥락성은 일반 커피생산의 착취적 가치사슬에서 인증 커피 생산에 따른 공유가치사슬로의 전환을 경험하였다. 이와 같은 전환은 글로벌 가치사슬에서 지역의 교섭력과 경쟁우위를 강화시킴으로써 지속적인 가치창출을 수반할 수 있을 것으로 기대되었다. 그러나 실질적으로 농가, 중개인, 외국 가공업체와 같은 지역 이해관계자간 이윤 분배의 위계성이 더욱 심화되는 현상이 나타났다. 이와 같은 현상의 원인은 크게 두 가지 측면에서 나타났다.첫째, 다국적기업, 중개인, 농가 간 전속관계의 형성은 이해관계자에게 안정적인 시장을 확보해 주었지만, 다국적 기업이 전속 커피 중개인을 통해 농가를 간접적으로 통제함으로써 이해관계자 간 위계가 더욱 심화되었다. 둘째, 공유가치창출의 명목으로 농가로 전가된 사회적 및 생태적 비용이 농가의 경제적 이윤 획득을 악화시킨 반면에 다국적기업으로의 비대칭적이고 위계적인 이윤 분배를 가져왔다. 그 결과, 닥락성에 보다 위계적이고 종속적인 글로벌 가치사슬이 형성되었다. The main aim of the research attempts to identify value relations appropriated and realized in the coffee frontier of Vietnam by investigating the ways in which it is integrated into coffee global value chains driven by multinational companies, and to provide some implications of the integration of the frontier into sustainable coffee global value chains for creating shared value in Dak Lak, Vietnam. Recently Dak Lak has gone through the transition of value relations from exploitative value chains based upon conventional coffee production into shared value chains relied upon the production of sustainable or certified coffee in Dak Lak. The transition has been expected to result in sustainability in the creation of value by enhancing regional competitive advantages and regional bargaining power in global value chains driven by multinational companies. However, the reality has shown the intensification of hierarchical profits allocation among stakeholders such as farmer, middlemen, and multinational companies in the region. The main reasons for this could be found in two perspectives. Firstly, the formation of exclusive relations among farmers, middlemen, and processors has led to stakeholders to secure market, but resulted in the intensification of hierarchy among them in global value chain, because multinational companies could control indirectly over the farming system through exclusive middlemen. Secondly, social and ecological costs imputed by multinational companies to coffee farmers in the name of creating shared value has deteriorated the economic profits of stakeholders such as farmers and middlemen. As a result, it has led to the configuration of systematically hierarchical and subordinated global value chain in Dak Lak.