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RISS 인기검색어
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- 저자 : Sandeep Kumar Tata (검색결과 3 건)
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Tata, Sandeep Kumar,Jung, Jihye,Kim, Yoon‐,Ha,Choi, Jun Young,Jung, Ji‐,Yul,Lee, In‐,Jung,Shin, Jeong Sheop,Ryu, Stephen Beungtae BLACKWELL 2016 PLANT BIOTECHNOLOGY JOURNAL Vol.14 No.1
<P><B>Summary</B></P><P>Geranylgeranyl pyrophosphate synthase (GGPS) is a key enzyme for a structurally diverse class of isoprenoid biosynthetic metabolites including gibberellins, carotenoids, chlorophylls and rubber. We expressed a chloroplast‐targeted <I>GGPS</I> isolated from sunflower (<I>Helianthus annuus</I>) under control of the cauliflower mosaic virus 35S promoter in tobacco (<I>Nicotiana tabacum</I>). The resulting transgenic tobacco plants expressing heterologous <I>GGPS</I> showed remarkably enhanced growth (an increase in shoot and root biomass and height), early flowering, increased number of seed pods and greater seed yield compared with that of <I>GUS</I>‐transgenic lines (control) or wild‐type plants. The gibberellin levels in <I>HaGGPS</I>‐transgenic plants were higher than those in control plants, indicating that the observed phenotype may result from increased gibberellin content. However, in <I>HaGGPS</I>‐transformant tobacco plants, we did not observe the phenotypic defects such as reduced chlorophyll content and greater petiole and stalk length, which were previously reported for transgenic plants expressing gibberellin biosynthetic genes. Fast plant growth was also observed in <I>HaGGPS</I>‐expressing <I>Arabidopsis</I> and dandelion plants. The results of this study suggest that <I>GGPS</I> expression in crop plants may yield desirable agronomic traits, including enhanced growth of shoots and roots, early flowering, greater numbers of seed pods and/or higher seed yield. This research has potential applications for fast production of plant biomass that provides commercially valuable biomaterials or bioenergy.</P>
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Tata, Sandeep Kumar,Choi, Jun Young,Jung, Ji-Yul,Lim, Ka Yung,Shin, Jeong Sheop,Ryu, Stephen Beungtae Elsevier 2012 INDUSTRIAL CROPS AND PRODUCTS Vol.40 No.-
<P><B>Highlights</B></P><P>► The <I>SRPP</I> promoter induces gene expression primarily in laticiferous tissues. ► The <I>SRPP</I> promoter responds to external conditions including light, tapping, and cold. ► The <I>SRPP</I> promoter is suitable for the latex-specific expression of a target gene(s) such as key rubber biosynthetic genes.</P> <P><B>Abstract</B></P><P><I>Hevea brasiliensis</I> is an important plant species currently cultivated for the commercial production of natural rubber. As the demand for rubber continues to increase, it is important to identify alternative sources of natural rubber and to increase plant rubber content using molecular approaches. <I>Taraxacum kok-saghyz</I>, a Russian dandelion, produces natural rubber that is of high quality. In this study, the <I>SMALL RUBBER PARTICLE PROTEIN</I> (<I>SRPP</I>) promoter from <I>H. brasiliensis</I> was characterized to determine its suitability for the expression of latex-specific genes in <I>Taraxacum brevicorniculatum</I> which is another Russian dandelion species of <I>T. kok-saghyz</I> from the similar geographical areas. Studies using transgenic <I>Taraxacum</I> plants carrying the <I>SRPP</I> promoter<I>::β-glucuronidase</I> (<I>GUS</I>) sequence indicate that the <I>SRPP</I> promoter does induce gene expression primarily in laticiferous tissues. Additionally, the promoter was regulated by various external conditions including light, tapping, and cold. These findings suggest that the <I>SRPP</I> promoter will be a useful molecular tool for the manipulation of gene expression in the laticiferous tissues of <I>Taraxacum</I> plant species.</P>
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Seasonal Variation of Rubber Production in Russian Dandelion, Taraxacum kok-saghyz, Grown in Korea
Sandeep Kumar Tata,홍승백(Seung Baek Hong),배성우(Sung Woo Bae),박종찬(Jong-Chan Park),Stephen Beungtae Ryu 한국자원식물학회 2022 한국자원식물학회지 Vol.35 No.3
Russian dandelion, Taraxacum kok-saghyz L.E.Rodin, is an important alternative source of natural rubber. Thus, it is critical to identify the best season for natural rubber production and harvesting for this species. In this study, T. kok-saghyz was grown in experimental fields in Korea to check the variation in rubber content with the change in the season. Studies using T. kok-saghyz plants indicate that spring and autumn are the best seasons for rubber accumulation. For several reasons, our study suggests that planting in autumn and harvesting in late spring is the best strategy for economic cultivation in Korea. Our data will be useful for future commercialization of rubber production from T. kok-saghyz because time will be saved if the plant is grown and harvested in the optimum season.
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