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      • SCOPUSKCI등재
      • KCI등재

        A missense mutation in the coding region of the toll-like receptor 4 gene affects milk traits in Barki sheep

        Sallam Ahmed M. 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.4

        Objective: Milk production is one of the most desirable traits in livestock. Recently, the toll-like receptor (TLR) has been identified as a candidate gene for milk traits in cows. So far, there is no information concerning the contribution of this gene in milk traits in sheep. This study was designed to investigate the TLR 4 gene polymorphisms in Barki ewes in Egypt and then correlate that with milk traits in order to identify potential single nucleotide polymorphisms (SNPs) for these traits in sheep. Methods: A part of the ovine TLR 4 gene was amplified in Barki ewes, to identify the SNPs. Consequently; Barki ewes were genotyped using polymerase chain reaction-single strand conformation polymorphism protocol. These genotypes were correlated with milk traits, which were the daily milk yield (DMY), protein percentage (PP), fat percentage (FP), lactose percentage, and total solid percentage (TSP). Results: Age and parity of the ewe had a significant effect (p<0.05 or p<0.01) on DMY, FP, and TSP. The direct sequencing identified a missense mutation located in the coding sequence of the gene (rs592076818; c.1710C>A) and was predicted to change the amino acid sequence of the resulted protein (p.Asn570Lys). The association analyses suggested a significant effect (p<0.05) of the TLR genotype on the FP and PP, while the DMY tended to be influenced as well (p = 0.07). Interestingly, the presence of the G allele tended to increase the DMY (+40.5 g/d) and significantly (p<0.05 or p<0.01) decreased the FP (–1.11%), PP (–1.21%), and TSP (–7.98%). Conclusion: The results of this study suggested the toll-like receptor 4 (TLR4) as a candidate gene to improve milk traits in sheep worldwide, which will enhance the ability to understand the genetic architecture of genes underlying SNPs that affect such traits. Objective: Milk production is one of the most desirable traits in livestock. Recently, the toll-like receptor (TLR) has been identified as a candidate gene for milk traits in cows. So far, there is no information concerning the contribution of this gene in milk traits in sheep. This study was designed to investigate the <i>TLR 4</i> gene polymorphisms in Barki ewes in Egypt and then correlate that with milk traits in order to identify potential single nucleotide polymorphisms (SNPs) for these traits in sheep.Methods: A part of the ovine <i>TLR 4</i> gene was amplified in Barki ewes, to identify the SNPs. Consequently; Barki ewes were genotyped using polymerase chain reaction-single strand conformation polymorphism protocol. These genotypes were correlated with milk traits, which were the daily milk yield (DMY), protein percentage (PP), fat percentage (FP), lactose percentage, and total solid percentage (TSP).Results: Age and parity of the ewe had a significant effect (p<0.05 or p<0.01) on DMY, FP, and TSP. The direct sequencing identified a missense mutation located in the coding sequence of the gene (rs592076818; c.1710C>A) and was predicted to change the amino acid sequence of the resulted protein (p.Asn570Lys). The association analyses suggested a significant effect (p<0.05) of the TLR genotype on the FP and PP, while the DMY tended to be influenced as well (p = 0.07). Interestingly, the presence of the G allele tended to increase the DMY (+40.5 g/d) and significantly (p<0.05 or p<0.01) decreased the FP (–1.11%), PP (–1.21%), and TSP (–7.98%).Conclusion: The results of this study suggested the toll-like receptor 4 (<i>TLR4</i>) as a candidate gene to improve milk traits in sheep worldwide, which will enhance the ability to understand the genetic architecture of genes underlying SNPs that affect such traits.

      • KCI등재

        Oncovascular Surgery and the Making of the Oncovascular Surgeon

        Kareem Sallam,Hussein Khairy 대한혈관외과학회 2019 Vascular Specialist International Vol.35 No.4

        Patients diagnosed with tumors that are located in vicinity of the major axial vessels are inconsistently managed because they are often considered unresectable. The management of these patients remains at the periphery of various established sub-specialties that are prevalent under the current medical specialty. These patients are required to be treated by an oncovascular surgeon. This article discusses some key points in understanding the reasons for establishing a specialty branch, oncovascular surgery, to treat complex tumors with vessel invasion. This article also reviews important issues about leadership and how to train and educate oncovascular surgeons.

      • KCI등재

        Genetic Variation of Stem Characters in Wheat and Their Relation to Kernel Weight underDrought and Heat Stresses

        Ahmed Sallam,Mervat Hashad,El-Sayed Hamed,Mohamed Omara 한국작물학회 2015 Journal of crop science and biotechnology Vol.18 No.3

        The frequency, duration, and severity of heat and drought stresses singly or in combination can be aggravated by climate change. Under these stresses, the stored carbohydrates in stems play an important role in grain filling in wheat (Triticum aestivum L.) post-anthesis. This study examines the genetic variation of stem characters in wheat and their role in supporting grain weight under favorable and drought, combined drought, and heat conditions. Field experiments were conducted with seven spring wheat genotypes along with their F1 and F2 progenies. Stem diameter (SD), stem density (SDN), and stem weight (SW) were measured in 21 F1 (half-diallel analysis) and 12 F2 (3 x4 North Carolina Design II) crosses. Among stem characters, SD was significantly correlated with thousand kernel weight (TKW) and grain yield per spike (GYPS) in all environments in F1 and F2. All characters showed high heritability estimates. For stem characters, the highest heritability was found for SD in both generations. High positive phenotypic and genotypic correlations were found between stem characters and both TKW and GYPS. The correlation between reductions due to stress (%) in stem characters and reduction due to stress in TKW and GYPS confirmed the strong relationships between stem characters and yield attributes in the stressed environments. We conclude that stem characters, especially SD, may play an important role in sustaining grain filling under such conditions. This is possibly due to a greater stem capacity for assimilates storage post-anthesis and subsequent remobilization to the grains.

      • KCI등재

        Genetic Variation of Stem Characters in Wheat and Their Relation to Kernel Weight under Drought and Heat Stresses

        Ahmed Sallam,Mervat Hashad,El-Sayed Hamed,Mohamed I. Hassan 한국작물학회 2015 Journal of crop science and biotechnology Vol.18 No.5

        The frequency, duration, and severity of heat and drought stresses singly or in combination can be aggravated by climate change. Under these stresses, the stored carbohydrates in stems play an important role in grain filling in wheat (Triticum aestivum L.) post-anthesis. This study examines the genetic variation of stem characters in wheat and their role in supporting grain weight under favorable and drought, combined drought, and heat conditions. Field experiments were conducted with seven spring wheat genotypes along with their F1 and F2 progenies. Stem diameter (SD), stem density (SDN), and stem weight (SW) were measured in 21 F1 (half-diallel analysis) and 12 F2 (3 x 4 North Carolina Design II) crosses. Among stem characters, SD was significantly correlated with thousand kernel weight (TKW) and grain yield per spike (GYPS) in all environments in F1 and F2. All characters showed high heritability estimates. For stem characters, the highest heritability was found for SD in both generations. High positive phenotypic and genotypic correlations were found between stem characters and both TKW and GYPS. The correlation between reductions due to stress (%) in stem characters and reduction due to stress in TKW and GYPS confirmed the strong relationships between stem characters and yield attributes in the stressed environments. We conclude that stem characters, especially SD, may play an important role in sustaining grain filling under such conditions. This is possibly due to a greater stem capacity for assimilates storage post-anthesis and subsequent remobilization to the grains.

      • SCOPUSKCI등재

        The Multiparameter Spectral Theory of Symmetric Operators

        M.H. Sallam KYUNGPOOK UNIVERSITY 1999 Kyungpook mathematical journal Vol.39 No.2

        The spectral theory of symmetric operators is extended to the multiparameter operator L(λ) = T - Σ^(k)_(s=1)λ_(s)V_(s), where T, V_(s) are symmetric operators on Hilbert space H. Assuming that T is densely defined closed operator and V_(s), s = 1, …, k are bounded, we use standard deficiency index theory to treat the multiparameter case.

      • KCI등재
      • KCI등재후보

        The temperature dependent electrical transport in biphenyl derivatives

        M.M. Sallam,B.A. El-Sayed,A.A. Abdel-Shafi 한국물리학회 2006 Current Applied Physics Vol.6 No.1

        The d.c. electrical conductivity measurements as a function of temperature of 4,4-dibromobiphenyl (DBrBP), 4,4-dichlorobiphe-nyl (DClBP) and 4,4-dimethoxybiphenyl (DMOBP) compounds have been carried out. The eects of conformational change withinskeleton of these compounds upon optical or thermal excitation (S1 S0 para substituents on the electrical conductivityhave been discussed. The conduction processes were interpreted in the light of their molecular spectroscopic data of the infrared,ultraviolet and emission spectra as a hopping processes. The semiconducting properties of the investigated compounds were arisingfrom electron-delocalization via intramolecular interaction ofp-electrons of (C@ C) bonds and electron-donating groups inparaincreases the possibility of electron-delocalization and the interaction ofp-electron system..

      • KCI등재

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