Effects of Human Adipose-Derived Stem Cells in Regenerating the Damaged Renal Tubular Epithelial Cells in an Animal Model of Cisplatin-Induced Acute Kidney Injury

Kim, Saeyoon,Lee, Eung Bin,Song, In Hwan,Kim, Yong Jin,Park, Hosun,Kim, Yong Woon,Han, Gi Dong,Kim, Kyung Gon,Park, Yong Hoon Korean Society of Pediatric Nephrology 2015 Childhood kidney diseases Vol.19 No.2

Background: We conducted this experimental study to examine whether human adipose-derived stem cells (ADSCs) are effective in achieving a recovery of damaged renal tubular epithelial cells in an animal model of cisplatin-induced acute kidney injury using rats. Methods: To examine the in vitro effects of ADSCs in improving nephrotoxicity, we treated mouse renal tubular epithelial cells with both ADSCs and cisplatin mouse renal tubular epithelial cells. And we equally divided 30 male white Sprague-Dawley (SD) rats into the three groups: the control group (intraperitoneal injection of a sterile saline), the cisplatin group (intraperitoneal injection of cisplatin) and the ADSC group (intraperitoneal injection of cisplatin and the hADSC via the caudal vein). At five days after the treatment with cisplatin, serum levels of blood urine nitrogen (BUN) and creatinine were measured from each SD rat. We performed histopathologic examinations of tissue samples obtained from the kidney. Results: The degree of the expression of TNF-${\alpha}$ and that of Bcl-2 were significantly higher and lower respectively, in cisplatin group (P<0.05). Serum levels of BUN (P=0.027) and creatinine (P=0.02) were significantly higher in cisplatin group. On histopathologic examinations, there was a significant difference in the ratio of the renal injury between cisplatin group and ADSC group (P=0.002). Conclusion: The ADSCs might have a beneficial effect in regenerating the damaged renal tubular epithelial cells.

Pediatric headache: a narrative review

Kim, Saeyoon Yeungnam University College of Medicine 2022 Yeungnam University Journal of Medicine Vol.39 No.4

Headache is one of the most common neurological disorders in children and adults and can cause significant distress and disability in children and their families. The spectrum of pediatric headaches is broad, and the underlying etiology is variable. The symptoms and phenotypes of headaches in children may differ slightly from those in adults. It is important to have a good understanding of headaches in children and to distinguish between primary and secondary headaches through appropriate history assessment and neurological examination. Accurate diagnosis and appropriate drug selection are helpful for effective treatment. This article reviews headaches in children and adolescents, focusing on approaches for diagnosis and management.

대구 소아의 세균성과 무균성 뇌수막염

김세윤(Saeyoon Kim),이응빈(Eung-Bin Lee),박선영(Sun Young Park),김상훈(Sanghoon Kim),양영호(Youngho Yang),강화정(Hwajeong Kang),권순학(Soonhak Kwon) 대한소아신경학회 2014 대한소아신경학회지 Vol.22 No.1

목적: 뇌수막염으로 진단된 소아환자에서 세균성 뇌수막염의 조기진단을 위해 대구지역에서 발생한 소아 뇌수막염 환자의 임상양상과 원인 병원체 등을 조사하였다. 방법: 2012년 3월부터 2013년 7월까지 대구지역에서 세균성과 무균성 뇌수막염으로 진단되어 영남대학교병원 소아청소년과에서 치료를 받은 115명의 환자의 의무기록을 후향적으로 분석하였다. 내원 당시의 임상증상과 혈액 및 뇌척수액 검사결과, bacterial menigitis score (BMS), 합병증 등에 대해 조사하였다. 결과: 대상 환아 115명 중 세균성 뇌수막염은 9명, 무균성 뇌수막염은 106명이었다. 처음 내원당시 가장 흔한 증상은 발열이었고, 그 외에 두통, 구토, 경부강직 등이 있었다. 뇌척수액 검사에서 세균성 뇌수막염군에서 백혈구가 더 많았다 (1,423.8±1,980.4 vs. 120.0±161.6/mm3). 단백질도 세균성 뇌수막염군에서는 219.4±183.6 mg/dL, 무균성 뇌수막염에서는 42.4±27.0 mg/dL이었다. BMS는 세균성 뇌수막염군에서 유의하게 높았다. 세균성 뇌수막염군 환아의 44%가 뇌자기공명영상검사에서 이상소견 (2명은 뇌염, 2명은 경막하 삼출액)을 보였다. 세균성 뇌수막염군은 모두 항생제 치료를 받았고 대부분 3세대 세팔로스포린과 반코마이신이 이용되었다. 무균성 뇌수막염군에서도 49%에서 3세대 세팔로스포린으로 치료를 받았다. 결론: 두 군의 초기 임상증상은 비슷하지만, 뇌척수액 검사에서는 뚜렷한 차이를 보였다. BMS도 세균성 뇌수막염을 조기에 감별하는 데 도움이 될 수 있다. 무균성 뇌수막염의 유행시, 조기에 구분하여 불필요한 입원이나 항생제 치료를 줄일 수 있다. Purpose: The aim of this study was to investigate the clinical characteristics and causative organisms of meningitis in the Daegu region and seek a useful tool for the early prediction of bacterial meningitis in children. Methods: We retrospectively reviewed the medical records of 115 pediatric patients diagnosed with bacterial or aseptic meningitis at Yeungnam university hospital in Daegu from March 2012 to July 2013. We evaluated their clinical symptoms, laboratory findings, clinical courses, bacterial meningitis scores and complications. Results: The subjects included 106 with aseptic meningitis and 9 with bacterial meningitis. At the time of visit, fever was the most frequent symptom, followed by headache, vomiting and neck stiffness. In cerebrospinal fluid (CSF) analysis, white blood cell (WBC) count were higher in the bacterial meningitis group (1423.8±1980.4 vs. 120.0±161.6 mg/dL). Mean CSF protein was 219.4±183.6 mg/ dL in bacterial meningitis and 42.4±27.0 mg/dL in aseptic meningitis (P <0.001). Bacterial meningitis score (BMS) were higher in the group with bacterial meningitis. Abnormal radiological findings were found in 44% of the group with bacterial meningitis. Conclusion: Although the clinical features between the groups were similar, the CSF analysis revealed significant differences statistically. Furthermore, BMS could be helpful to predict bacterial meningitis in children. During the outbreak of aseptic meningitis, it might reduce unnecessary hospital admissions and antibiotic treatments.

발포제 종류에 따른 페놀 폼의 물성 연구

장서윤(SaeYoon Jang),김상범(Sangbum Kim) 한국가스학회 2016 한국가스학회지 Vol.20 No.2

본 연구에서는 LNG나 LPG등과 같은 초저온용 단열재로 사용되는 폴리우레탄 폼을 대체하기 위한 연구의 일환으로 레졸형 페놀수지를 사용하여 페놀 폼을 합성하였다. 페놀 수지 발포 폼을 합성하기 위해 HCFC-141b, HFC-365mfc와 HFC-227fa의 혼합물, n-pentane, cyclopentane, n-hexane 및 cyclohexane을 발포제로 사용하여 발포제에 따른 페놀수지 폼의 물성변화를 고찰하였다. 발포제로 cyclohexane을 사용하였을때 가장 우수한 단열성능과 압축강도를 나타내었으며 동일한 발포제를 사용하여 합성한 폴리우레탄 폼과의 내열성을 비교한 결과 폴리우레탄 폼에 비해 매우 우수한 내열성을 나타내었다. In this study, we synthesized a phenol foam using a resol-type phenol resin as a research for replacing the polyurethane foam used as an insulator for cryogenic temperature, such as LNG or LPG. Foaming agents for synthesizing a phenolic foam was used HCFC-141b or n-pentane, cyclopentane, n-hexane, cyclohexane and a mixture of HFC-365mfc and HFC-227ea respectively. Cyclohexane as a blowing agent exhibited the most superior insulating performance and compressive strength. The heat resistance of polyurethane foam and phenolic foam blown by the cyclohexane, was higher than polyurethane foam.

Next-Generation Sequencing for the Early Diagnosis of Adolescent Patients with Suspected Charcot-Marie-Tooth Disease: A Case Series

Jongdae Park,Sanghoon Kim,Ji Young Ahn,Chul Hyun Park,Saeyoon Kim 대한소아신경학회 2022 대한소아신경학회지 Vol.30 No.2

A Case of Cecal Volvulus Presenting with Chronic Constipation in Lissencephaly

Lee, Eun-Kyung,Kim, Ji Eun,Lee, Yun-Young,Kim, Saeyoon,Choi, Kwang Hea The Korean Society of Pediatric Gastroenterology 2013 Pediatric gastroenterology, hepatology & nutrition Vol.16 No.2

Cecal volvulus is uncommon in pediatric patients and there are few reports of cecal volvulus with cerebral palsy. Here, we report the case of a 19-year-old male patient who presented with abdominal distension, a history of cerebral palsy, refractory epilepsy due to lissencephaly, and chronic constipation. An abdominal x-ray and computed tomography without contrast enhancement showed fixed dilated bowel intensity in the right lower abdomen. Despite decompression with gastric and rectal tube insertion, symptoms did not improve. The patient underwent an exploratory laparotomy that revealed cecal volvulus. Cecal volvulus usually occurs following intestinal malrotation or previous surgery. In this patient, however, intestinal distension accompanying mental disability and chronic constipation resulted in the development of cecal volvulus. We suggest that cecal and proximal large bowel volvulus should be considered in patients presenting with progressive abdominal distension combined with a history of neuro-developmental delay and constipation.

Content Analysis of YouTube Videos on the Effect of Vitamin C on Common Cold

Park Donghwi,Kwak Sang Gyu,Kim Saeyoon,Chang Min Cheol 대한가정의학회 2023 Korean Journal of Family Medicine Vol.44 No.6

Background: With the growth of the Internet, social media platforms have emerged as major sources of medical in-formation. We assessed the reliability, quality, and accuracy of the most-viewed YouTube videos containing infor-mation on the effect of vitamin C on the common cold.Methods: The YouTube videos were searched on August 1, 2022, using the keywords: (“ascorbic acid” OR “vitamin C” OR “Sodium Ascorbate” OR “L-ascorbic”) AND “common cold”. The 30 most-viewed videos were included in our study. The reliability and quality of the videos were analyzed using modified DISCERN and Global Quality Scales, respectively. When the videos included at least one correct or inaccurate scientific statement about the ef-fect of vitamin C on the common cold, they were classified as accurate or misleading videos, respectively; those without any pertinent information were considered neither accurate nor misleading. If a video contained both ac-curate and inaccurate statements, it was classified as misleading.Results: Of the 30 most-viewed videos, 73% were unreliable, and 67% contained misleading information and were of a poor quality. Of these 30 videos, 14 videos were produced and posted by customers who were not specialized in medicine or nutrition. Moreover, these videos were of significantly lower reliability, quality, and accuracy than those produced by nutrition or fitness channels or by medical or nutrition professionals.Conclusion: The reliability, quality, and accuracy of videos uploaded by non-professionals were low. Therefore, video creators should upload reliable, high-quality videos to ensure the dissemination of accurate medical infor-mation.

Medial Lemniscus Lesion in Pediatric Hemiplegic Patients without Corticospinal Tract and Posterior Thalamic Radiation Lesion

Jung, Yong Jae,Jang, Sung Ho,Yeo, Sang Seok,Lee, Eunsil,Kim, Saeyoon,Lee, Dong Gyu,Kim, Han Sun,Son, Su Min S. Karger AG 2012 European neurology Vol.67 No.4

<P>Abstract</P><P><I>Objectives:</I> Using diffusion tensor imaging (DTI), we investigated the state of medial lemniscus (ML), corticospinal tract (CST), and posterior thalamic radiation (PTR), which were expected as probable reasons for clinical hemiplegia in pediatric patients, especially those who showed impaired fine motor control and proprioception, but no definite motor weakness or spasticity. <I>Methods:</I> We recruited 13 hemiplegic patients and 8 age-matched healthy control subjects. Fractional anisotropy (FA) and apparent diffusion coefficient (ADC) for the bilateral ML, CST, and PTR were calculated and compared between the affected hemisphere of the patient (AP), the unaffected hemisphere of the patient (UP), and the mean value of the bilateral hemispheres in control subjects (MC). <I>Results:</I> FA and ADC values for the CST and PTR did not differ significantly between the AP, UP, and MC subgroups (p > 0.05). However, the FA value for the ML in AP showed a significant decrease, compared with that in UP (p = 0.012) and MC (p = 0.047). DTT for the CST and PTR showed preserved integrity and ML in the UP also had continuity to the cortex; however, ML in AP showed disruption. <I>Conclusions:</I> Using DTI, we demonstrated that the ML lesion might be related to clinical hemiplegia in pediatric patients.</P><P>Copyright © 2012 S. Karger AG, Basel</P>

Clinical Efficacy of a Top-down Approach for Children with a First Febrile Urinary Tract Infection

Jang, Kyung Mi,Lim, Myung Hee,Park, Yong Hoon,Kim, Saeyoon Korean Society of Pediatric Nephrology 2017 Childhood kidney diseases Vol.21 No.2

Purpose: The aim of this study was to determine the clinical characteristics, frequency of renal abnormalities and benefits of a top-down approach in children with their first febrile urinary tract infection (UTI). Methods: We reviewed 308 patients retrospectively who were admitted to Yeungnam University Hospital and were treated for their first febrile UTI from February 2006 to December 2013. We performed a comparative analysis of laboratory findings and results of imaging techniques including a Tc-99m dimercaptosuccinic acid (DMSA) renal scan. Results: Among the patients, 69% (213/308) were males, and 90% (277/308) had their first UTI episode during infancy. A DMSA renal scan was performed on all patients, and showed positive findings in 60% (184/308) of cases. Laboratory indices of inflammation were significantly higher in the DMSA-positive group (P< 0.05). There was a statistically significant difference in the age distribution between the two groups. In the DMSA-positive group, 165 patients underwent voiding cystourethrography (VCUG), and 58 (35%) cases demonstrated vesicoureteral reflux. In total, 110 patients in the DMSA-positive group, underwent repeat scanning at 6 months; 33 children (30%) demonstrated static scarring, but 77 (70%) had improved completely. The concordance of the ultrasonography (US) and VCUG was low. Older patients had more renal scarring. Conclusion: DMSA is a sensitive method for assessing the severity of inflammation and kidney injury. However, the ability of US to predict renal parenchymal damage was limited. A top-down approach in children with their first febrile UTI showed significant value.