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Glucose와 Phosphate가 제거된 M-TALP 배지에서의 난구세포 공배양에 의한 임신율 향상에 관한 연구
정범식,장우현,이문희,김지연,방지호,김규현,서태광,Chung, B.S.,Chang, W.H.,Lee, M.H.,Kim, J.Y.,Bang, J.H.,Kim, K.H.,Suh, T.K. 대한생식의학회 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.1
The beneficial effect of glucose and phosphate ions in culture medium on the development of human embryos in vitro has not been fully elucidated. The purpose of this study was to evaluate the influence of fertilization and culture of embryos in glucose/phosphate-free m-TALP medium on pregnancy rates in IVF-ET program. The patients in 244 IVF-ET cycles received GnRH agonist + HMG regimens. A does of 10,000 IU HCG was administered when two or more dominent follicles reached 18mm in diameter. Thirty-six hours after HCG, oocytes were recovered transvaginally using ultrasound guidance. Aspirated oocytes were matured for 4 to 6 h in TCM-199 supplemented with 10% follicular fluid (FF). Insemination was carried out with 50,000 motile spermatozoa in TCM-199 + 10% FF or m-TALP + 5% FF + 5% fetal cord serum (FCS) according to experimental design. After 6 h, oocytes were washed 3 to 4 times and cultured in each fresh medium. After 20 h, oocytes were freed from cumulus/corona cells and examined for the presence of pronuclei. Fertilized oocytes were transferred into each co-culture drops and cultured for further incubation. On day 3, embryo transfer was performed with grade 1 and 2 embryos. Monolayers for co-culture of embryos were prepared by plating $1{\times}10^5$ cumulus cells/ml in 10ul drop of TCM-199 + 10% FF or m-TALP + 5% FF + 5% FCS media 24 h prior to the onset of co-culture. Development to 4 to 16 cell stage was observed at 70x magnification following two days of incubation. Pregnancy was confirmed by detecting increasing serum ${\beta}$-hCG concentrations for 11 days following embryo transfer. Data were analyzed by ${\chi}^2$-test. Oocytes from 244 IVF-ET cycles were randomized. The number of cycles and mean age of patients were 97 and 147, 31.3 yrs and 31.2 yrs for TCM-199 (control) and m-TALP groups, respectively. The mean number of retrieved oocytes/cycle, fertilization rates, number of embryos transferred/ET and pregnancy rates were 11.1 and 10.3, 65.1% and 67.3%, 4.1 and 4.7, 28.9% and 43.8% for TCM-199 and m-TALP groups, respectively. Differences in the pregnancy rates were found between control and m-TALP groups (p<0.05). The pregnancy rate of patients divided according to maternal age groups of ${\leq}30$, 31-35, $36{\leq}$ were 44.4% and 49.0%, 26.1% and 41.3%, 29.2% and 41.2% for control and m-TALP groups, respectively. These data indicate that culture of human embryos in glucose/phosphate-free m-TALP medium improves pregnancy rates.
서종환(J.H. Suh),서명원(M.W. Suh),양원호(W.H. Yang) 한국자동차공학회 1996 한국자동차공학회 춘 추계 학술대회 논문집 Vol.1996 No.11_1
The joint characteristics are necessary to be determined in the early stage of the automotive structural analysis. Identification of joints in vehicle body has been researched until the recent years. In this study, the joint characteristics of vehicle structure were expressed as condensed forms from the full joint stiffness and mass matrix. The condensed joint stiffness and mass matrix were applied to typical T-type and Edge-type joints, and the usefulness was confirmed. Also, it was verified by the application to the lower B-pillar of vehicle body<br/>
Tamoxifen May Inhibit Hepatic Fibrogenesis by Suppressing the Activation of Hepatic Stellate Cells
( J. A. Kim ),( Y. H. Chung ),( M. K. Jang ),( J. K. Lee ),( M. H. Choi ),( S. H. Kim ),( S. H. Ryu ),( J. W. Shin ),( K. M. Kim ),( Y. S. Lim ),( Y. S. Lee ),( D. J. Suh ) 대한간학회 2006 Clinical and Molecular Hepatology(대한간학회지) Vol.12 No.3(S)
박종민(J.M. Park),서성원(S.W. Suh),권영국(Y.G. Kwon),박주범(J.B. Park),김현우(H.W. Kim),김건태(G.T. Kim) 대한전기학회 2010 대한전기학회 학술대회 논문집 Vol.2010 No.7
This paper presents a series of processes of designing, manufacturing and testing of AC 22.9㎸ three core XLPE submarine cables with optical fiber unit. To verify the mechanical performance of the submarine cables, CAE (Computer Aided Engineering) method have been applied to structural design of the submarine cable and the corresponding mechanical tests have been performed.
김명한(M.H.Kim),임석현(S.H.Lim),서명원(M.W.Suh),배동호(D.H.Bae) 한국자동차공학회 1997 한국자동차공학회 춘 추계 학술대회 논문집 Vol.1997 No.11_2
Automobile body structure is generally assembled using various spot welded box sectional members. Especially, in the case of bus, the shape of window pillar joint is assembled in T-type. The T-type member need to solve the problems such as high stress concentration, low fatigue strength and structural rigidity.<br/> In this study, a study on the optimum design of bus window pillar joint for improving such problems by FEM analysis and experiments is performed.