한국재래산양의 육성사료 개발을 위한 농산 부산물 중 사과박과 미강의 이용

황보순,안종호,이주삼,조익환 한국영양사료학회 1999 韓國營養飼料學會誌 Vol.23 No.4

본 실험에서는 볏짚, 사과박, 미강 등의 농산부산물 및 시판사료의 혼합 비율을 A구는 60:0:0:40, B구는 60:0:30:10, C구는 30:30:30:10, D구는 0:60:30:10으로 조제하여 한국 재래산양에게 급여하였다. 사료섭취량과 영양소 소화율 및 제 1위내 발효 성상을 조사하였으며 이에 따른 재래산양의 육성사료로서 농산부산물의 활용 가능성을 제시하고자 실시하였다. 그 결과를 요약하면 다음과 같다. 1. 시험사료의 화학적 조성분은 조단백질 함량이 8.2∼9.5%이었고 ADF와 NDF 함량은 A구가 각각 32.2와 65.8%로 가장 높았고 D구가 l8.3와 36.5%으로 가장 낮은 경향을 보였으나, 조지방과 NSC는 D구가 9.2와 39.5%로 가장 높았다. 2. 1일 두당 건물섭취량은 A, C 및 D구가 각각 455.6, 446.5 및 418g으로 B구의 313.1g 보다 유의하게 높았다. 3. 건물, 유기물, 조단백질, ADF 및 NDF 소화율은 ADF와 NDF 함량이 적고 NSC 함량이 높은 사과박 혼합구가 높았다. 4. 질소 축적량과 축적율은 사과박 혼합구가 높았고 B구가 가장 낮았다. 5. pH 및 NH₃-N농도는 각각 6.1∼6.8, 22.6∼24.4㎎/100㎖의 범위로서 처리구간 pH의 유의한 차이는 없었으나 사과박 혼합구에서 NH₃-N 농도가 유의하게 높았다. 6. Acetate와 propionate의 비율은 사과박 혼합구가 낮게 나타났다. In this study, rice straw, apple pomace and rice bran of agricultural by-products and commercial diets were mixed and fed to Korean native growing goats in 4 different ratios (A 60:0:0:40, B 60:0:30:10, C 30:30:30:10, D 0:60:30:10). Dry matter intake, digestibilities of nutrients and ruminal fermentation characteristics were investigated and using those data, the possibilities of application of agricultural byproducts in Korean native goats were suggested. The results are as follows. 1. Crude protein contents of experimental diets in 4 different treatments were similar in the range of 8.2∼9.5%. The contents of ADF(acid detergent fiber) and NDF(neutral detergent fiber) were highest in A (32.2, 65.8%) and lowest in D (18.3, 36.5%). Ether extracts and NSC (non-structural carbohydrates) were highest in D (9.2, 39.5%). 2. Dry matter intakes per head and day were significantly higher in A, C and D (455.6, 446.5, 418g) than 313.1g of B. 3. Digestibilities of dry matter, organic matter, crude protein, ADF and NDF were higher in apple pomace treatments that contained higher NSC and less ADF and NDF than rice straw treatments. 4. Nitrogen retention was higher in apple pomace treatments wheras that of B was lowest among all treatments. 5. pH values of 6.1∼6.8 were similar in all treatments however NH₃-N concentrations in the range of 22.6∼24.4 ㎎/100㎖ were significantly higher in apple pomace treatments. 6. A/P ratios (Acetate/Propionate ratio) were lower in apple pomace treatments than those of other treatments

Tonal domains and nonfinality effects in the Samchuk dialect of Korean

Young-Shik Hwangbo 한국음운론학회 2003 음성·음운·형태론 연구 Vol.9 No.1

Hwangbo, Young-Shik. 2003. Tonal domains and nonfinality effects in the Samchuk dialect of Korean. Studies in Phonetics, Phonology and Morphology 9.1. 223-246. This paper deals with tones of the Samchuk dialect of Korean, focusing on typical tonal patterns of nouns. I claim that the underlying forms of Korean tone dialects are marked by tonal boundaries, which later define tonal domains. I argue, following Gim (1988, 1999), that in most cases the positions of tonal boundaries are predictable from Middle Korean side-dots, and thus that all modern tone dialects have the same location of tonal boundaries. In addition, I argue, following Hwangbo (2001), that the different tone realization between dialects results from different constraint ranking. I show that the interesting tonal phenomena of the Samchuk dialect can be explained in a principled manner if we have recourse to the tonal boundaries and tonal domains. More specifically, I show that 'post-accenting' and 'pre-accenting' observed in the Samchuk dialect can be explained as nonfinality effects within tonal domains. (Sungkyul University)

Genome-Wide Association Study Reveals Distinct Genetic Susceptibility of Thyroid Nodules From Thyroid Cancer

Hwangbo, Yul,Lee, Eun Kyung,Son, Ho-Young,Im, Sun-Wha,Kwak, Soo-Jung,Yoon, Ji Won,Kim, Min Joo,Kim, Jeongseon,Choi, Hoon Sung,Ryu, Chang Hwan,Lee, You Jin,Kim, Jong-Il,Cho, Nam H,Park, Young Joo Issued for the Endocrine Society by the Williams & 2018 The Journal of clinical endocrinology & metabolism Vol.103 No.12

AN EVALUATION OF THE SOLAR RADIO BURST LOCATOR (SRBL) AT OVRO

HwangBo, J.E.,Bong, Su-Chan,Cho, K.S.,Moon Y.J.,Lee, D.Y.,Park, Y.D.,Gary Dale E.,Dougherty Brian L. The Korean Astronomical Society 2005 Journal of The Korean Astronomical Society Vol.38 No.4

The Solar Radio Burst Locator (SRBL) is a spectrometer that can observe solar microwave bursts over a wide band (0.1-18 GHz) as well as detect the burst locations without interferometry or mechanical scanning. Its prototype has been operated at Owens Valley Radio Observatory (OVRO) since 1998. In this study, we have evaluated the capability of the SRBL system in flux and radio burst location measurements. For this, we consider 130 microwave bursts from 2000 to 2002. The SRBL radio fluxes of 53 events were compared with the fluxes from USAF/RSTN and the burst locations of 25 events were compared with the optical flare locations. From this study, we found: (1) there is a relatively good correlation (r = 0.9) between SRBL flux and RSTN flux; (2) the mean location error is about 8.4 arcmin and the location error (4.7 arcmin) of single source events is much smaller than that (14.9 arcmin) of multiple source events; (3) the minimum location error usually occurred just after the starting time of burst, mostly within 10 seconds; (4) there is a possible anti-correlation (r = -0.4) between the pointing error of SRBL antenna and the location error. The anti-correlation becomes more evident (r=-0.9) for 6 strong single source events associated with X-class flares. Our results show that the flux measurement of SRBL is consistent with that of RSTN, and the mean location error of SRBL is estimated to be about 5 arcmin for single source events.

Utilization of house fly-maggots, a feed supplement in the production of broiler chickens.

Hwangbo, J,Hong, E C,Jang, A,Kang, H K,Oh, J S,Kim, B W,Park, B S The Academy 2009 Journal of environmental biology Vol.30 No.4

<P>Recent studies have suggested the utilization of maggots as a feed supplement forenhanced broiler performance. Maggots, which are a major dietary source of protein, appear during the biodegradation of chicken droppings using house flies. The objective ofthe present study was to investigate the effect of maggot supplementation on the meat quality and growth performance of broiler chickens. A total of 600 one-day-old male commercial broiler chicks (Ross) were randomly assigned into 5 treatment groups consisting of 40 replicates of 3 birds. The birds were fed either a basal diet or the basal diet supplemented with 5.0, 10.0, 15.0 and 20.0% maggots. Overall, broiler chicken performance was influenced by the optimal amino acid profile; high protein (63.99%) and essential amino acid content (29.46%), or high protein digestibility (98.50%) of the maggots. Maggot supplementation caused linear increases in live weight gain but not the feed conversion ratio. The diets of 10 and 15% maggots was the most efficient in terms of average weight gain forthe 4-5 week old broiler chickens (p<0.05). It also significantly increased dressing percentage, breast muscle, and thigh muscle (p<0.05). No differences were observed forliver abdominalfat, or meat color, and the crude protein contents of breast muscle were constant. However, in the maggot-fed broilers, breast muscle lysine and tryptophan levels increased significantly as compared to the birds fed the basal diet (p<0.05). These results indicate that feeding diets containing 10 to 15% maggots in chicken dropping after biodegradation can improve the carcass quality and growth performance of broiler chickens.</P>

Synthesis of Single Crystalline Titanium Oxide and Sodium Titanate Nanorods Via Salt-Assisted Ultrasonic Spray Pyrolysis

Hwangbo, Y.,Yoo, J.-H.,Lee, Y.-I. PAS 2017 ARCHIVES OF METALLURGY Vol.62 No.2

<P>The simple and continuous synthesis of single crystalline anatase titanium dioxide and sodium titanate nanorods by a salt-assisted ultrasonic spray pyrolysis method is demonstrated. This method does not require expensive precursors, long reaction time, and physical templates or surfactant. In addition, its continuous nature makes it a suitable method for the large-scale preparation. Moreover, the effect of a salt concentration in a starting solution on material properties, including morphology and phase of the synthesized products was systematically investigated. The synthesized nanorods had one-dimensionality, a single crystalline and the average diameter of 12.3 nm with dual phases of titanium dioxide and sodium titanate by FE-SEM, XRD, HR-TEM as well as FFT-converted SAED pattern analysis.</P>

Effects of Progesterone and 17β-Estradiol under Presence or Absence of FBS on Plasminogen Activators Activity in Porcine Uterine Epithelial Cells

Hwangbo, Yong,Lee, Mi-Rim,Cheong, Hee-Tae,Yang, Boo-Keun,Park, Choon-Keun The Korean Society of Developmental Biology 2018 발생과 생식 Vol.22 No.4

The present study was conducted to investigate the regulatory mechanism of plasminogen activators (PAs) activation by $17{\beta}$-estradiol ($E_2$) and progesterone ($P_4$) in porcine uterine epithelial cells (pUECs). pUECs were collected from porcine uterine horn and cultured at 80% confluence. Then, 0.1% (v/v) DMSO, 20 ng/mL $E_2$, and $P_4$ with or without fetal bovine serum (FBS) treated to cultured cells for 24 hours. The supernatants were used for measurement of PAs activity and expression of urokinase-type PA (uPA), tissue-type PA (tPA), uPA specific receptor (uPAR), and type-1 PA inhibitor (PAI-1) mRNA were analyzed by real-time PCR. The expression of PAs-related genes was not affect by steroid hormones in both of serum treatment groups. However, PAs activity was increased by treatment of $E_2$ compared to 0.1% DMSO treatment in serum-free group (p<0.05). Then, $E_2$ and $P_4$ were diluted with 0.002% (v/v) DMSO for reduction of its effect and treated to cultured cells without FBS. Only tPA mRNA was significantly increased by $E_2$ treatment (p<0.05). PAs activity was enhanced in $E_2$ treated group compared to control groups (p<0.05). These results indicate that serum-free condition is more proper to evaluate effect of steroid hormones and activation of PAs in pUECs was mainly regulated by estrogen. These regulation of PAs activation may be associated with uterine remodeling during pre-ovulatory phase in pigs, however, further studies are needed to investigate precise regulatory mechanism.

Association test for rare variants using the hamming distance

Hwangbo, Suhyun,Jang, Jin Young,Oh, Bermseok,Okazaki, Atsuko Imai,Ott, Jurg,Park, Taesung Inderscience Enterprises Ltd. 2018 International journal of data mining and bioinform Vol.21 No.4

Treatment of Epidermal Growth Factor (EGF) enhances Nuclear Maturation of Porcine Oocytes and Stimulates Expression of ER/Golgi Transport Proteins

Hwangbo, Yong,Oh, Hae-In,Lee, Sang-Hee,Cheong, Hee-Tae,Yang, Boo-Keun,Park, Choon-Keun The Korean Society of Developmental Biology 2017 발생과 생식 Vol.21 No.2

This study was conducted to investigate stimulatory effect of epidermal growth factor (EGF) on nuclear maturation and the expression level of EGF-receptor (EGFR), GM-130 (a marker of Golgi apparatus), transport protein Sec61 subunit beta ($Sec61{\beta}$), and coatomer protein complex subunit gamma 2 (COPG2) in porcine oocytes. The cumulus-oocyte complexes were collected from follicle with 3-6 mm in diameter. They were incubated in medium with/without EGF for 22 h (IVM I) and subsequently incubated hormone-free medium with/without EGF for 22 h (IVM II). Nuclear maturation state was checked by aceto-orcein stain. Protein expression of EGFR, GM-130, $Sec61{\beta}$, and COPG2 were measured by immunofluorescence. In results, nuclear maturation of oocytes in EGF non-treated oocytes were significantly lower than EGF-treated groups at IVM I or IVM II stage (P<0.05), whereas maturational rate in EGF treatment groups at both of IVM stage was higher in among the all treatment groups (P<0.05). EGFR, GM-130, $Sec61{\beta}$ and COPG2 were expressed in the cytoplasm of oocytes. Especially, GM-130 and EGFR were strongly expressed, but $Sec61{\beta}$ and COPG2 were weakly expressed in cortical area of cytoplasm. The protein level of GM-130, $Sec61{\beta}$, and COPG2 were significantly higher in the EGF-treated groups (P<0.05). However EGFR was no difference between non EGF-treated groups and control. In conclusion, EGF plays an important role in the systems for oocyte maturation with endoplasmic reticulum and Golgi apparatus. In addition, the protein levels of $Sec61{\beta}$ and COPG2 could be changed by EGF in the porcine oocytes during maturation.

Mathematical model to optimize design of integrated utility supply network and future global hydrogen supply network under demand uncertainty

Hwangbo, Soonho,Lee, In-Beum,Han, Jeehoon Elsevier 2017 APPLIED ENERGY Vol.195 No.-

<P>Existing energy-efficient management and development of alternative energy infrastructure are the most important issues in modern industry. The former has been mainly studied in terms of optimal design of utility supply network. The latter has been consistently researched in the field of strategic planning of future global hydrogen supply network. In this work, we develop an integrated network model of huge chemical industrial complexes to combine various utility supply networks with a global hydrogen supply network. To construct an integrated network model, the steam methane reforming process is used as a linkage between the two networks. The developed model is a two-stage stochastic mixed integer linear programming that optimizes total cost and also considers demand uncertainty about water, electricity, steam, and hydrogen that are consumed in each network. Finally, a case study in South Korea is conducted to validate the proposed model; it suggests reasonable scenarios to decision makers. (C) 2017 Elsevier Ltd. All rights reserved.</P>