돈유래(豚由來) Escherichia coli의 항생물질내성(抗生物質耐性) 및 전달성내성인자(傳達性耐性因子)에 관(關)하여

탁연빈,정길택,Tak, Ryun Bin,Chung, Gill Taik 대한수의학회 1976 大韓獸醫學會誌 Vol.16 No.2

One hundred and sixty one Escherichia coli strains isolated from 24 swine (11 swine fed with feedstuffs containing 7.5mg/kg of tetracycline and 13 swine not received antibiotic) were studied for the drug resistance and distribution of R factors. About 42 per cent of E. coli strains isolated from pigs of a herd fed with tetracycline (TC)-containing feeds were resistant to TC, streptomycin(SM), sulfisomidine(SA), ampicillin (AP) and kanamycin (KM), alone or in combination thereof, but none of the swine not receiving antibiotic containing feedstuffs excreted E. coli resistant to these drugs, Among resistant strains, 18.2% were found to be singly resistant to TC, whereas 81.8% were resistant to two or more antibiotics. The most common pattern was the triple resistant to TC, SM and SA(30.3%), and follolwed by double ones to TC and SM(24.2%). About one half of resistant strains carried R factors which were tranferable to the recipients by conjugation. In spite of feeding with feedstuffs containing only TC, high incidences of multiple resistance and R factors were observed in the E. coli isolated from these swine.

학술발표 연제 및 초록 : 고유견 삽사리의 보호육성에 관한 연구

탁연빈(Ryun Bin Tak),하지홍,김종봉 한국수의공중보건학회 1991 예방수의학회지 Vol.15 No.4

가축(계) 적혈구 침강속도에 관한 연구

유창준,탁연빈,하성진,Yu Chang Jun,Tak Ryun Bin,Ha Seoung Jin 대한수의사회 1981 대한수의사회지 Vol.17 No.1

The adult New Hampshire female chickens were phlebotomized and the blood heparinized was centrifuged. The packed cell volume was made and the hematocrit, per centage of the blood that is red cells, was reschuffled of $10\%,30\%,50\%, and 70\%$

Salmonella choleraesuis의 증균(增菌)에 관한 연구 -Tetrathionate Broth Base에 대한 옥도용액(沃度溶液)의 함량(含量)이 증균효과(增菌效果)에 미치는 영향-

정길택,탁연빈,Chung, Gill Taik,Tak, Ryun Bin 대한수의학회 1976 大韓獸醫學會誌 Vol.16 No.1

The growth of Sal. choleraesuis and its kunzendorf variety in tetrathionate broth containing various amounts of iodine solution was studied and compared with that of Sal. typhi, Sal. typhimurium and E. coli. The results obtained were as followings. 1. When 2.0 ml of iodine solution, normal amount, was added to 100 ml of tetrathionate broth base, the number of Sal. choleraesuis decreased rapidly until 24 hours after inoculation and slightly increased 48 hours after inoculation. The numbers of Sal. choleraesuis var. kunzendorf and E. coli decreased rapidly and none of the organisms recovered 24 and 48 hours after inoculation, respectively. The growth of Sal. typhi and Sal. typhimurium, however, was not inhibited at all. 2. When 4.0 ml of iodine solution was added, to 100 ml of tetrathionate broth base, the growth of all the organisms was inhibited, among which Sal. choleraesuis, Sal. choleraesuis var. kunzendorf, and E. coli were not recovered 24 and 48 hours after inoculation. 3. When reduced amounts of iodine solution, 1.0 ml and 0.5 ml, were added to 100 ml of tetrathionate broth base, the growth of all the organisms was not inhibited.

돼지 전염성 위축성 비염의 임상학적 및 세균학적 연구

김봉환,탁연빈,조길재,장희경,Kim, Bong-hwan,Tak, Ryun-bin,Cho, Gil-jae,Jang, Hee-kyung 대한수의학회 1991 大韓獸醫學會誌 Vol.31 No.4

Clinical and bacteriological observations on infectious atrophic rhinitis of swine were conducted in order to obtain some basic information for the clinical and immnnological control of the disease prevailing in the republic. Samples were collected from nasal cavities of 135 4~12 week old pigs from 12 herds and from turbinates of 199 slaughtering pigs from 14 swine herds to investigate the prevalence of Bordetella bronchiseptica and/or Pasteurella multocida in the nasal cavities of the pigs. On the examination of nasal swabs by cultural techniques and of gross lesion of snouts of slaughtering pigs, all the swine herds investigated were found to be affected by atrophic rhinitis and a total of 84 B bronchiseptica and 139 P multocida cultures were isolated from the nasal cavities of the pigs. Of the 199 slaughtering pigs, some 48% revealed gross pathological lesion typical of atrophic rhinitis and the prevalence of B bronchiseptica and P multocida were 27.6% and 46.7%, respectively. Biochemical properties, antimicrobial susceptibilities, serological characteristics and toxigenicity of the isolates of B bronchiseptica and P multocida were investigated.

집단 개사육농장에서의 Canine Brucellosis 발생 및 PCR-RFLP를 이용한 분리주의 특성조사

김종완,이영주,탁연빈,Kim, Jong-Wan,Lee, Young-Ju,Tak, Ryun-Bin 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.1

A total of 260 dogs were randomly selected from two different treed kennels that brucellosis has occurred (group 1, 126 dogs), and random selected breed kennel (group 2, 134 dogs), and monitored for Brucella canis (B. canis) by 2-mercaptoethanol rapid slide agglutination test (2ME-RSAT) and bacterial culture method. For the differentiation, PCR-RFLP using omp-31, wbkA and per genes used for 52 of B canis strains (strain I) isolated in this study and 3 of B. canis strains (strain II) isolated in 1994 in Korea. 2ME-RSAT revealed that 63/126 dogs (50.0%) and 12/134 dogs (9.0%) were positive in group I and group II, respectively. Bacterial culture revealed that 47/126 dogs (37.3%) and 5/134 dogs (3.7%) were positive in group I and group II, respectively. As the results of PCR-RFLP, $\underline{omp}-31$ was amplified from all Brucella spp, except B. abortus. All B. canis isolates showed unique PCR-RFLP pattern following digestion with Bmel8I. However, all Brucella spp. showed the same PCR-RFLP pattern following digestion with SalI. PCR-RFLP analysis of wbkA revealed that all Brucella spp. showed the same pattern following digestion with HindIII. PCR-RFLP analysis of per revealed that B. abortus 544 and B. melitensis 63/9 showed the same pattern, but different from B. suis and B. canis following digestion with HindIII.

영남지방 돼지에서 분리한 Pasteurella multocida의 협막혈청형 및 항균제 감수성 조사

조길재,김봉환,탁연빈,Cho, Gil-jae,Kim, Bong-hwan,Tak, Ryun-bin 대한수의학회 1989 大韓獸醫學會誌 Vol.29 No.4

The capsular serogroupes and drug susceptibility of 111 isolates of Pasteurella multocida from pigs with atrophic rhinitis and pneumonic lesions were investigated. Of the 111 P multocida isolates, 42 were from lung lesions, 47 from nasal turbinate lesions and the remaining 22 from the nasal swabs. P multocida isolates were typed for capsular serogroupes A by hyaluronidase inhibition of capsule and D by acriflavine auto-agglutination. Most isolates(64.9%) were type A, 23.4% were type D and the remaining 11.7% were untypable. Resistance to triple sulfa(97.3%) was most frequent, followed by resistance to tiamulin(71.2%), tylosin(56.8%), streptomycin(36.9%), and neomycin(36.0%). The majority of the organisms were susceptible in order of prevalence to baytril(100%), ampicillin(98.2%), linsmycin(97.3%), colistin(97.3%), cephalothin(94.6%), gentamicin(93.7%), amikacin(92.3%), tetracycline(91.9%), trimethoprim/sulfamethoxazole(91.0%), and kanamycin(90.1%). No differences in drug resistance in relation to capsular serogroupes of P multocida and the origin of lesions were noted. A high prevalence of multiple drug resistance was observed and the most common resistant patterns were Sss, Tm, Ty(12.6%) and Sm, Sss, Tm, Ty(8.1%) patterns.

PCR 기법을 이용한 Mycoplasma gallisepticum의 검출

이영주,김기석,김종완,탁연빈,Lee, Young-ju,Kim, Ki-seuk,Kim, Jong-wan,Tak, Ryun-bin 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.1

A species-specific 760 base pair(bp) BamHI to EcoRI DNA fragment(fMG-2) of lipoprotein gene was isolated from a Mycoplasma gallisepticum(M gallisepticum) genomic library. Based on the DNA sequence data of fMG-2, a pair of 25bp primers was synthesized. When used in the polymerase chain reaction(PCR), 732bp DNA products were amplified from 6 standard strains and 10 field isolates of M gallisepticum, but not from 2 Mycoplasma synoviae and 7 other Mycoplasma species. The lower detection limit was 100fg of the genomic DNA. Identity of the PCR products was confirmed by comparison of patterns of restriction endonuclease analysis with AseI, DraI, EcoRV and SspI.

학술발표 연제 및 초록 : 대구지역 동물 및 사람의 Toxoplasma 항체조사

문무홍(Moo Hong Moon),탁연빈(Ryun Bin Tak) 한국수의공중보건학회 1991 예방수의학회지 Vol.15 No.4

Staphylococcus aureus의 增殖과 enterotoxin産生에 미치는 pH, 溫度 및 食品添加物의 影響

Gab Soo Chung(鄭甲洙),Ryun Bin Tak(卓鍊斌) 한국예방수의학회 1993 예방수의학회지 Vol.17 No.1

In this study, field isolates of Staphylococcus aureus from the fresh chicken carcasses and from the pathological lesions of chickens with staphylococcosis were subjected to determine the growth characteristics and the immunological toxin types. In addition, effective food additives were screened to inhibit the growth and enterotoxin production of S. aureus in order to promote the food safety. When one hundred and twenty fresh chicken carcasses obtained from 6 poultry processing plants at Kyong-gi area from March to November in 1990 and chicken specimens diagnosed as staphylococcosis during 4 years from 1989 to 1992 were examined, total of 83 and 106 strains of S. aureus isolated, respectively. Among those, 4 of 83 isolates from the fresh chicken carcasses, and 74 of 106 strains from chickens with staphylococcosis were typed as enterotoxigenic strains of S. aureus, respectively. All these enterotoxigenic strains produced an enterotoxin type D only, and these enterotoxins from the 48 strains tested all caused an emetic response in cat. To determine the production of staphylococcal enterotoxin, 10 isolates and 4 reference strains(ATCC 13565, 14458, 19095 and 23235) of S. aureus were inoculated into NZ-medium at a concentration of 10⁴ cells/ml and incubated at various temperature. At 10℃, all fourteen strains did not grow and produce enterotoxins until 240 hours. At 15℃, 3 strains(ATCC 13565, 14458 and 1 isolate) produced an enterotoxin after 72 hours of incubation, and 8 strains(ATCC 23235 and 7 isolates) produced after 96 hours of incubation, respectively. Nevertheless the remaining 3 strains(ATCC 19095 and 2 isolates) did not produce unitl 96 hours. At 37℃, eleven out of 14 strains produced enterotoxin after 4 to 7 hours of incubation, and each 1 strain produced after 8, 9 and 10 hours, respectively. When ground chicken meats inoculated with S. aureus were incubated at 22℃, all 14 strains produced enterotoxin in the raw meat after 24 hours of incubation, and 12 strains produced enterotoxin in the heated meat(100℃, 30 minutes) after 6 to 12 hours. When 21 food additives were tested for inhibitory effects on the growth and enterotoxin production of S. aureus in brain heart infusion broth, butyl p-hydroxybenzoate(BH), butylated hydroxyanisole(BHA) and lactic acid(LA) showed a significant inhibitory effects. The lower pH of media, the greater inhibiotry effects of BH, BHA and LA were observed. In addition, a combination of BH or BHA with NaCI had a greater inhibitory effect than when either BH or BHA were used alone. Complete inhibition of S. aureus growth occured in BHI broth with 156.3ppm BH or 200ppm BHA. In combination with 2% NaCI, however, 100ppm BH or 150ppm BHA exerted bacteriocidal effects. The bacteriocidal effect was also observed when 0.4% LA was supplemented in the culture medium. When 0.2% LA, 0.2% sorbic acid, 25ppm BH+2% NaCI, 25ppm BHA+2% NaCI, or 0.15% erythorbic acid+0.26% potassium sorbate+0.01% sodium nitrite+2% NaCI were added to raw chicken meat production of enterotoxin from the inoculated S. aureus was completely inhibited until 48 hours of storage at 22℃.