家畜 腸內細菌의 抗生物質에 對한 感受性 및 傳達性 耐性因子에 關한 硏究

Ryun Bin Tak(卓鍊斌),Young Hong Kim(金永洪),Cheong Kyu Park(朴淸圭) 한국예방수의학회 1979 예방수의학회지 Vol.3 No.1

Five hundred and nine strains of Esherichia coli isolated from domestic animals and 23 strains of Salmonella isolated from 237 pigs were tested for drug resistance and distribution of R plasmids. Seventy two per cent of E. coli strains isolated from domestic animals fed with antibiotics-containing feeds were resistant to tetracycline(TC), streptomycin(SM), chloramphenicol(CM), ampicillin(AM) and kanamycin(KM), alone or in combination thereof, but about 2% of E. coli strains isolated from domestic animals received no antitiotics were resistant to TC and SM. Among resistant strains, 45.4% were found to be singly resistant to TC, SM or KM, whereas 54.6% were resistant to two or more antibiotics. The most common resistance pattern was double resistant to TC and SM(33.7%) and followed by triple ones to TC, SM and AM(4.9%). About 67% of resistant E. coli carried R factors which were transferable to the recipients by conjugation. Seventy eight per cent of Salmonella strains isolated from pigs were resistant to TC, SM, AM, and KM, alone or in combination thereof, and the most common resistance pattern was resistant to TC and SM(38.9%) slimilary to E. coli and about one half of resistant Salmonella carried R factors. There was no difference in numbers of TC-resistant Coliform for 6 weeks after stopping administration of antibiotics, but they were decreased to one seventh at the 13th week.

鷄由來 病原性 大腸菌에 관한 硏究 4. 大腸菌感染 病鷄로부터 分離한 大腸菌의 R plasmid 分離 및 性狀調査

Ki Seuk Kim(金基錫),Ryun Bin Tak(卓練斌) 한국예방수의학회 1984 예방수의학회지 Vol.8 No.2

For the detection and preliminary characterization of plasmid DNA, attempts were made to extract R plasmid from host strains of Escherichia coli ML 1410 transconjugant haboring the plasmid by conjugation. Following the method of SDS-lysozyme treatment, four different incompatibility group plasmids and one field plasmid originated from chicken were isolated from the host strains of E. coli transconjugant. And Five different R plasmid bands were visualized with agarose gel electrophoresis.

鷄由來 病原性 大腸菌에 관한 硏究 1. 大腸菌感染 病鷄로부터 分離한 大腸菌의 生化學的性狀 및 血淸型 調査

Ki Seuk Kim(金基錫),Ryun Bin Tak(卓練斌) 한국예방수의학회 1983 예방수의학회지 Vol.7 No.2

Four hundred and eighty eight strains of Escherichia coli isolated from chicken with pericarditis, perihepatitis, airsacculitis, salpingitis, yolk sac disease, arthritis, peritonitis or acute septicemia during 4 years from 1978 to 1981 were tested for their biochemical properties and 0 serogroups. Biochemical properties of 488 strains of E. coli tested were in accordance with classification standard of Edwards and Ewing. By using E. coli antisera of 24 different 0 groups, 224(61.9%) of the 362 strains tested were classified into 18 0 groups. The most prevalent 0 group found was 0 128 (22.7%), and followed by 0 1alb(7.2%), 0 124(5.8%), 0 83(3.9%), 0 2a2b(3.6%), 0 6(3.3%), and 0 26(3.0%); these strains typed for 7 0 groups comprised 81.8% of the typable strains and 49.5% of the total 362 strains.

鷄由來 病原性 大腸菌에 관한 硏究 3. 大腸菌感染 病鷄로부터 分離한 Citrate 陽性 大腸菌의 生化學的 性狀 및 Citrate 利用能의 傳達

Ki Seuk Kim(金基錫),Ryun Bin Tak(卓練斌) 한국예방수의학회 1984 예방수의학회지 Vol.8 No.2

Twenty citrate-positive strains of Escherichia coli were obtained from chicken with colibacillosis. With the exception of citrate utilization biochemical properties of these strains of E. coli were in accordance with citrate-negative strains of E. coli of chicken origin. The citrate-utilizing ability of 18 out of these twenty citrate-positive strains of E. coli was transferred by conjugation to recipient strain of E. coli ML 1410. And the conjugal transfer of citrate-utilizing ability was thermosensitive and concurrent with transfer of drug resistance.

美國農科大學 實習場運營現況 및 實態調査

朴恒均,卓鍊斌,李起運 慶北大學校 1984 論文集 Vol.38 No.-

原發性肝癌의 血淸 α1-Fetoglobulin에 關한 硏究 : 嶺南地方의 肝癌例에 對하여

鄭俊謨,卓鍊斌,鄭泰浩,全燾基,鄭克守 慶北大學校 醫科大學 1972 慶北醫大雜誌 Vol.13 No.1

飼料添加劑의 微生物汚染에 關하여

朴壽璟,卓鍊斌 慶北大農學誌編輯委員 1986 慶北大農學誌 Vol.4 No.-

市販 ?料添加劑에 대한 微生物學的 汚染程度를 알아보기 위하여 國內에서 販賣되고 있는 비타민과 鐄物質 飼料添加劑 36個 品目 總 81例의 試料를 供試하여 一般細菌 및 大腸菌群의 汚染狀態를 檢?하고 아울러 試料로부터 分離한 83株의 大腸菌群에 대한 Am等 8種의 抗菌性樂劑에 대한 耐性頻度 및 耐性程道를 파악하였으며 耐性菌에 있어서는 耐性樣相과 R plasmid의 分布를 調査하여 다음과 같은 成績을 얻었다. 一般細菌은 試料 81例 모두 陽性이었으며 大腸菌群은 81例中 14例(17.3%) 에서만 陽性이었다. 一般細菌數의 分布는 g當 10미만에서부터 1,400,000까지 多樣하였으며 그 中 100~1,000/g이 34例 (42%)로 가장 많았고 大腸菌群에 있어서는 一般細菌의 汚染度가 높을수록 分離率이 높았으며 總 18個 製造會社中 6個社 (33.3%)의 製品에서 陽性이었다. 供試菌 83株中 41株 (49.4%)가 fecal coliform이었다. 供試菌에 대한 樂劑別 耐性菌出現率은 sulfadimethoxine (Sa)에 대해 92.8%로 가장 높았고 다음으로 streptomycin (Sm)에 67.5%, tetracycline (Tc)에 50.6%, kanamycin (Km)에 26.5%, chloramphenicol (Cm)에 18.1%, ampicillin (Am)에 15.7% 順이었으며 nalidixic acid (Na)와 gentamicin (Gm)에는 全株가 感受性이었고 各供試 樂劑에 있어서 non-fecal coliform에 비하여 fecal coliform의 耐性菌出現率이 높았다. 供試菌의 最小發育沮止濃度 (minimum inhibitory concentration, MIC) 分布는 Am 및 Km에 대하여 MIC가 3,200㎍/㎖ 以上인 高度의 耐性을 가진 菌이 各各 7株 및 3株이었으나 대부분의 耐性菌은 그 MIC가 25㎍/㎖이었고, Cm, Sm 및 Tc에 대한 耐性菌의 대부분은 25㎍/㎖에서 400㎍/㎖의 範圍이었다. 供試菌 83株中 79株 (95.2%)가 供試한 樂劑 1種 以上에 耐性을 가졌으며 耐性型別로는 SaSm 耐性型 및 Sa 單劑耐性型이 各各 12株 (14.5%)로 가장 많았고 다음으로 SaSmTc型 10株 (12%), SaSmTcKm型 7株 (8.4%), SaTc型 7株 (8.4%) 및 SaSmKm형 6株 (7.2%)의 順이었으며 總 19種의 耐性型이 관찰되었다. 耐性傅達試驗 結果 耐性菌 79株中 32株(40.5%)가 傅達性 R plasmid를 保有하고 있었으며 多劑耐性菌일수록 耐性傅達率이 높았다. 供試 樂劑別 耐性傅達頻度는 Am (100%) 및 Cm (80%)에서 매우 높고, 다음으로 Tc (38.1%), Sa (18.2%), Sm (17.9%) 및 Km (4.5%)의 順이었다. Eighty one products from 36 kinds of vitamin and mineral feed supplement collected during August, 1984 to February, 1985 were examined for microbiological contamination. In addition, 83 strains of coliform isolated from the samples were tested for the resistance to 8 kinds of antimicrobial drugs and distribution of R plasmid. General bacteria were detected in all of samples tested. Bacterial population was varied from less than 10 per gram of the sample to 1,400,000 per gram and 34 (42%) of 81 samples were contaminated with 100 to 1,000 cells per gram. Coliform isolation, which was more frequent in samples with larger number of general bacteria, was possible in 14 (17.3%) out of 81 samples tested and 6 (33.3%) out of 18 companies were coliform positive in their products. Forty one (49.4%) out of 83 coliform isolates were fecal coliform. The frequency of resistant strains was the highest to sulfadimethoxine (Sa) with 92.8% and followed by streptomycin (Sm, 67.5%), tetracycline (Tc, 50.6%), kanamycin (Km, 26.5%), chloramphenicol (Cm, 18.1%) and ampicillin (Am, 15.7%). No strain was resistant to nalidixic acid (Na) and gentamicin (Gm). The resistance frequency of fecal coliform strains were higher compare to non-fecal coliform strains. There were minimum inhibitory concentration (MIC) of 3,200㎍/㎖ or higher in 7 strains to Am, 3 to Sm and 3 to Km, and 70 strains had MIC of 1,600㎍/㎖ of higher to Sa while Tc had MICs from 1.6㎍/㎖ to 400㎍/㎖. All strains had MICs of 6.3㎍/㎖ of lower to Na and 3.1㎍/㎖ of lower to Gm. Seventy nine (95.2%) of 83 strains were resistant to one or more drugs tested. The most frequent resistance patterns were SaSm (14.5%) and followed by SaSmTc (12%), SaSmTcKm(8.4%) SaTc (8.4%) and SaSmKm (7.2%) ; total 19 different patterns were noted. Thirty two (40.5%) of 79 resistant strains were transferred all of a part of their resistance to Escherichia coli ML 1410. The frequency of transferable resistance was high in Am (100%) and Cm (80%) while low in Tc (38.1%), Sa (18.2%), Sm (17.9%) and Km (4.5%).

原發性肝癌의 血淸 α_1-Fetoglobulin에 關한 硏究 : 嶺南地方의 肝癌例에 對하여

鄭俊謨,卓練斌,鄭泰浩,全燾基,鄭克守 慶北大學校 醫科大學 1972 慶北醫大誌 Vol.13 No.1

As a part of a series of study on serum α_1-fetoprotein, it was examind so far in 27 cases of the liver by double immunodiffusion in agar gel. Positive α_1-fetoprotein was seen only in 13 cases (48%). Age distribution, sex, history and its duration and hepatomegaly had no relation between positive and negative α_1-fetoprotein groups. In liver profile and isotopic scanning, however, 75% of positive α_1-fetoprotein cases showed patterns compatible with chronic liver disease while only 30%of negative α_1-fetoprotein cases did so. Alpha_1 fetoprotein antisera could be obtained from immunized rabbits through injections of positive α_1-fetoprotein serum.