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[PC-0006] Employing hyperspectral imaging for early detection of diseases in soybean
Rupesh Tayade(Rupesh Tayade ),Liny Lay(Liny Lay),Hogyun Kim(Hogyun Kim),Waleed Khan(Waleed Khan),Amit Ghimire(Amit Ghimire ),Mohammad Shafiqul(Mohammad Shafiqul ),Yoonha Kim(Yoonha Kim) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-
( Rupesh Tayade ),( Tiendung Nguyen ),( Sung Aeong Oh ),( Yong Sik Hwang ),( In Sun Yoon ),( Rupesh Deshmuk ),( Ki-hong Jung ),( Soon Ki Park ) 한국육종학회 2018 Plant Breeding and Biotechnology Vol.6 No.1
Temperatures that extend beyond normal levels of tolerance cause severe stress to plants, especially during the reproductive and grain filling/ripening stages. Heat stress leads to serious yield losses in many crop plants, including rice (Oryza sativa). In view of the current scenario of global climate change, frequent fluctuations and a significant increase in average temperatures will pose challenges to protecting those yields. Therefore, elucidating the molecular mechanisms that make crop plants more tolerant of heat, particularly in organs at the reproductive stage, is of utmost importance. Precise molecular information will be helpful for the manipulation and exploration of relevant genes for use in crop improvement programs. In this review, we highlight recent progress in research on the molecular responses to high temperatures in pollen and seed and provide a perspective on the development of heat tolerance in rice cultivars. The responsible mechanism is a very complex phenomenon that involves several biochemical and physiological changes, molecular responses, and a series of signal transductions. Improving our understanding requires detailed knowledge at various omics levels. Recent technological advancements have accelerated genomics, transcriptomics, and proteomics studies in rice, a model crop plant. Here, we discuss those technological and omics approaches being taken to investigate the heat tolerance mechanism, particularly in rice. In addition, we address the tools being used to identify key genes and QTLs that can then be utilized for molecular breeding and biotechnology.
Rupesh Kumar Mishra,Brajesh Kumar Pandey,Vijai Singh,Amita John Mathew,Neelam Pathak,Mohammad Zeeshan 한국미생물학회 2013 The journal of microbiology Vol.51 No.4
Twenty one isolates of Fusarium oxysporum f. sp. psidii (Fop),causing a vascular wilt in guava (Psidium guajava L.), were collected from different agro-ecological regions of India. The pathogenicity test was performed in guava seedlings,where the Fop isolates were found to be highly pathogenic. All 21 isolates were confirmed as F. oxysporum f. sp. psidii by a newly developed, species-specific primer against the conserved regions of 28S rDNA and the intergenic spacer region. RAPD and PCR-RFLP were used for genotyping the isolates to determine their genetic relationships. Fifteen RAPD primers were tested, of which five primers produced prominent, polymorphic, and reproducible bands. RAPD yielded an average of 6.5 polymorphic bands per primer,with the amplified DNA fragments ranging from 200–2,000bp in size. A dendrogram constructed from these data indicated a 22–74% level of homology. In RFLP analysis, two major bands (350 and 220 bp) were commonly present in all isolates of F. oxysporum. These findings provide new insight for rapid, specific, and sensitive disease diagnosis. However,genotyping could be useful in strain-level discrimination of isolates from different agro-ecological regions of India.
A relative assessment of sub grid scale models for large eddy simulation of co-axial combustor
Rupesh D. Shah,Hanumanthappa Patil,Jyotirmay Banerjee 대한기계학회 2012 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.26 No.6
Large eddy simulation (LES) of a co-axial combustor is reported. Accuracy of LES depends on the ability of the sub-grid scale (SGS)models to predict the turbulent viscosity. The sensitivity of LES results for different SGS models is established for a coaxial annular combustor. The radial, axial and tangential velocity distribution predicted by four sub-grid scale models is compared with the experimental results of Sommerfeld and Qiu. It is observed that the flow physics is captured more accurately by WALE model as compared to other SGS models. The predictions of WALE model are closer to the experimental results for all stations in the flow domain.
Rupesh Tayade, and Yoonha Kim 한국국제농업개발학회 2022 韓國國際農業開發學會誌 Vol.34 No.1
Chinese cabbage or pakchoi (Brassica Rapa subsp. Chinensis) is one of the most popular vegetables in Korea. It is grown and consumed throughout the year because it has many benefits. This study reports on the growth performance of pakchoi grown in the hydroponic system using a Food Juke Box (FJB) under different light compositions. The chlorophyll content (CC), root fresh weight (RFW), and shoot fresh weight (SFW) were measured. Further, various vegetation indices (VIs), such as modified chlorophyll absorption in reflectance index (MCARI1), normalized difference vegetation index (NDVI), optimized soil-adjusted vegetation index (OSAVI), and structure insensitive pigment index (SIPI), were employed to predict SFW. The study results indicated a significant increase in the CC of pakchoi with different light treatments compared with the control. The CC was highest under treatment with 70% white light, 20% blue light, and 10% red light (T1) than under treatment with 50% white light, 30% blue light, and 20% red light (T2). All used VIs showed significant differences with different light combination treatments. There was no significant change in RFW and SFW between the control and T1. However, a significant reduction in RFW and SFW was observed in T2 compared with the control. A comparison between T1 and T2 demonstrated that RFW and SFW were increased by 23% and 25%, respectively, compared with those in T2. The correlation result showed that SFW had a significant positive correlation with RFW (0.76***). Further, a significant negative correlation was observed with OSAVI (−0.25*), MCARI1 (−0.54***), and CC (−0.19*). In conclusion, our findings implied that different light combinations in pakchoi affected the photosynthetic pigments although they did not improve SFW. This research will pave the way for pakchoi production in hydroponics using smart farming FJB. This will further promote plant development, especially for domestic consumption, and help fulfill the growing demand for leafy vegetables.
Modeling and optimization of geometrical characteristics in laser trepan drilling of titanium alloy
Rupesh Goyal,Avanish Kumar Dubey 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.3
Laser drilling has become an alternative to drilling precise holes in advanced difficult-to-cut superalloys. Due to better hole quality and capability to generate macro-size holes, laser trepan drilling is becoming more popular as compared with laser percussion drilling. This paper presents a study of laser trepan drilling process performance in terms of geometrical quality characteristics, such as hole taper and circularity for drilling small diameter hole in difficult-to-cut Titanium alloy sheet. Due to involvement of different process parameters such as laser power, pulse width, pulse frequency, workpiece thickness, material composition, cutting speed, stand of distance and assist gas pressure, the laser cutting is a highly nonlinear and complex process. To handle this nonlinearity and complexity, genetic algorithm has been applied for the optimization. We used assist gas pressure, pulse width, pulse frequency and trepanning speed as input process parameters. The effect of significant process parameters on hole characteristics are discussed on the basis of data obtained through a well designed orthogonal array experimental matrix. Reliable empirical models have been developed for different quality characteristics. Improvements of 49% and 8% have been registered in hole taper and circularity, respectively, at optimum level of process parameters.
Thrust estimation of a flapping foil attached to an elastic plate using multiple regression analysis
Rupesh Kumar,Hyun Kyoung Shin 대한조선학회 2019 International Journal of Naval Architecture and Oc Vol.11 No.2
Researchers have previously proven that the flapping motion of the hydrofoil can convert wave energy into propulsive energy. However, the estimation of thrust forces generated by the flapping foil placed in waves remains a challenging task for ocean engineers owing to the complex dynamics and uncertainties involved. In this study, the flapping foil system consists of a rigid NACA0015 section undergoing harmonic flapping motion and a passively actuated elastic flat plate attached to the leading edge of the rigid foil. We have experimentally measured the thrust force generated due to the flapping motion of a rigid foil attached to an elastic plate in a wave flume, and the effects of the elastic plates have been discussed in detail. Furthermore, an empirical formula was introduced to predict the thrust force of a flapping foil based on our experimental results using multiple regression analysis.