Biosequestration, Transformation, and Volatilization of Mercury by Lysinibacillus fusiformis Isolated from Industrial Effluent

( Gupta Saurabh ),( Richa Goyal ),( Jashan Nirwan ),( Swaranjit Singh Cameotra ),( Nagaraja Tejoprakash ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.5

In the present study, an efficient mercury-tolerant bacterial strain (RS-5) was isolated from heavy-metalcontaminated industrial effluent. Under shake flask conditions, 97% of the supplemented mercuric chloride was sequestered by the biomass of RS-5 grown in a tryptone soy broth. The sequestered mercuric ions were transformed inside the bacterial cells, as an XRD analysis of the biomass confirmed the formation of mercurous chloride, which is only feasible following the reaction of the elemental mercury and the residual mercuric chloride present within the cells. Besides the sequestration and intracellular transformation, a significant fraction of the mercury (63%) was also volatilized. The 16S rRNA gene sequence of RS-5 revealed its phylogenetic relationship with the family Bacillaceae, and a 98% homology with Lysinibacillus fusiformis, a Gram-positive bacterium with swollen sporangia. This is the first observation of the sequestration and volatilization of mercuric ions by Lysinibacillus sp.

An interesting story of a clone

Richa Juneja,Karthika Kundil Veetil,Gopila Gupta,Prasad Dange,Hara Prasad Pati 대한혈액학회 2020 Blood Research Vol.55 No.2

Hemitruncus Arteriosus With Anomalous Origin of the Right Coronary Artery From the Right Pulmonary Artery and Unilateral Absence of the Left Pulmonary Artery

Mohini Gupta,Viralam S Kiran,Suraj Gowda,Richa Kothari,Vimal Raj 아시아심장혈관영상의학회 2024 Cardiovascular Imaging Asia Vol.8 No.2

Hemitruncus arteriosus, anomalous origin of a pulmonary artery from the aorta, is a rare congenital heart disease frequently accompanied by other cardiovascular anomalies. In this case report, we present an exceedingly rare case of hemitruncus arteriosus in an 18-year-old male patient presenting with cyanosis and chest pain. Echocardiography raised a suspicion for aorto-pulmonary window; this was confirmed on cardiac computed tomography angiography. A magnetic resonance imaging study was performed for functional assessment. The resulting diagnosis was hemitruncus arteriosus with aberrant origin of the right coronary artery from the right pulmonary artery and unilateral absence of a pulmonary artery.

Acute megakaryoblastic blast crisis as a presentation manifestation of chronic myelogenous leukemia

Jenna B Bhattacharya,Richa Gupta,Amit Samadhiya 대한혈액학회 2017 Blood Research Vol.52 No.2

Therapeutic Value of Hippophae rhamnoides L. Against Subchronic Arsenic Toxicity in Mice

S.J.S. Flora,Richa Gupta 한국식품영양과학회 2005 Journal of medicinal food Vol.8 No.3

The present study was planned to investigate the therapeutic efficacy of Hippophae rhamnoides L. against the toxic effects of arsenic in mice. H. rhamnoides L. is used as an herbal remedy for gastric ulcers, burns, and some skin and allergic diseases. Twenty-five Swiss albino mice were exposed to arsenic (25 ppm) in drinking water for 3 months. After 3 months different fruit extracts of H. rhamnoides L. (500 mg/kg for 10 days) were administered, the animals were sacrificed,and blood and tissues were assayed for various biochemical indicators of oxidative stress and whether arsenic was removed from tissues. Treatment with different fruit extracts of H. rhamnoides L. showed significant protection from arsenic inhibition of blood -aminolevulinic acid dehydratase activity and restored blood reduced glutathione levels. Other hematologic variables like white blood cell counts, hemoglobin, and hematocrit were partially protected by supplementation with a water extract of H. rhamnoides L. (HF-WRT). Significant protection was also observed in altered hepatic, renal, and brain reduced/oxidized glutathione ratio and thiobarbituric acid-reactive substances levels. The aqueous extract of H. rhamnoides L.(HF-WRT) also provided protection against parameters indicative of liver injury such as aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase activities. There was also no effect on blood and tissue arsenic concentrations observed except some moderate depletion of blood arsenic concentrations, suggesting that the drug has no ability to chelate intracellular arsenic. It can be concluded from these results that post-treatment with an aqueous extract of H. rhamnoides L. (HF-WRT) significantly protects against arsenic-induced oxidative stress but does not chelate arsenic, suggesting it may have a beneficial role as a supplementing agent during chelation of arsenic by other means.

Acute megakaryoblastic blast crisis as a presentation manifestation of chronic myelogenous leukemia

Jenna B Bhattacharya,Richa Gupta,Amit Samadhiya 대한혈액학회 2017 Blood Research Vol.51 No.2

Cadaveric anatomy of the lumbar triangular safe zone of Kambin’s in North West Indian population

Chiman Kumari,Tulika Gupta,Richa Gupta,Vishal Kumar,Daisy Sahni,Anjali Aggarwal,Neelkamal 대한해부학회 2021 Anatomy & Cell Biology Vol.54 No.1

A three dimensional triangular space ‘the Kambin’s triangle (KT)’ present on the dorsolateral aspect of the intervertebral disc, is considered to be a safe area for transforaminal approaches. It allows access to the exiting and traversing nerve roots, the thecal sac and to the intervertebral disc spaces. Our aim was to calculate the area of the triangle by measuring the height and base at all the intervertebral spaces bilaterally in the lumbar region in North West Indian cadavers and to assess the diameter of circle inscribed within this triangle which will correspond to the size of cannula inserted for the minimally invasive transforaminal approaches in this population. Five randomly chosen adult cadavers were used for this study. After clearing the area, the exiting nerve was identified. The height and base of the bony KTs (n=40) were measured with the help of digital Vernier’s calliper (accuracy 0.02 mm) to calculate the area of the KT. There is a steady increase in the area of the bony KT reaching maximum at the level of L4-5 intervertebral space. Statistically there were no differencesin the calculated areas between right and left side. The mean diameter of inscribed circle within the triangle also showed gradual increase from 5.82 mm at L1-2 level, reaching maximum value of 7.26 mm at L4-5 level on the right side while on the left side the values were 5.66 mm and 8.16 mm respectively. Careful anatomical consideration is of utmost importance in transforaminal approaches during surgical or interventional procedures in this region. Cannula having external diameter ranging 6–8 mm is recommended for any interventional approach through Kambin’s space.

Shelterin Proteins and Cancer

Patel, Trupti NV,Vasan, Richa,Gupta, Divanshu,Patel, Jay,Trivedi, Manjari Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.8

The telomeric end structures of the DNA are known to contain tandem repeats of TTAGGG sequence bound with specialised protein complex called the "shelterin complex". It comprises six proteins, namely TRF1, TRF2, TIN2, POT1, TPP1 and RAP1. All of these assemble together to form a complex with double strand and single strand DNA repeats at the telomere. Such an association contributes to telomere stability and its protection from undesirable DNA damage control-specific responses. However, any alteration in the structure and function of any of these proteins may lead to undesirable DNA damage responses and thus cellular senescence and death. In our review, we throw light on how mutations in the proteins belonging to the shelterin complex may lead to various malfunctions and ultimately have a role in tumorigenesis and cancer progression.

Fetomaternal outcomes in pregnant women with congenital heart disease: a comparative analysis from an apex institute

Soniya Dhiman,Aparna Sharma,Akanksha Gupta,Richa Vatsa,Juhi Bharti,Vidushi Kulshrestha,Satyavir Yadav,Vatsla Dadhwal,Neena Malhotra 대한산부인과학회 2024 Obstetrics & Gynecology Science Vol.67 No.2

Objective With advancements in cardiac surgical interventions during infancy and childhood, the incidence of maternal congenital heart disease (CHD) is increasing. This retrospective study compared fetal and cardiac outcomes in women with and without CHD, along with a sub-analysis between cyanotic versus non-cyanotic defects and operated versus non-operated cases.Methods A 10-year data were retrospectively collected from pregnant women with CHD and a 1:1 ratio of pregnant women without any heart disease. Adverse fetal and cardiac outcomes were noted in both groups. Statistical significance was set at <i>P</i><0.05.Results A total of 86 pregnant women with CHD were studied, with atrial septal defects (29.06%) being the most common. Out of 86 participants, 27 (31.39%) had cyanotic CHD. Around 55% of cases were already operated on for their cardiac defects. Among cardiovascular complications, 5.8% suffered from heart failure, 7.0% had pulmonary arterial hypertension, 8.1% presented in New York Heart Association functional class IV, 9.3% had a need for intensive care unit admission, and one experienced maternal mortality. Adverse fetal outcomes, including operative vaginal delivery, mean duration of hospital stay, fetal growth restriction, preterm birth (<37 weeks), low birth weight (<2,500 g), 5-minute APGAR score <7, and neonatal intensive care unit admissions, were significantly higher in women with CHD than in women without heart disease.Conclusion Women with CHD have a higher risk of adverse fetal and cardiac outcomes. The outcome can be improved with proper pre-conceptional optimization of the cardiac condition, good antenatal care, and multidisciplinary team management. Objective With advancements in cardiac surgical interventions during infancy and childhood, the incidence of maternal congenital heart disease (CHD) is increasing. This retrospective study compared fetal and cardiac outcomes in women with and without CHD, along with a sub-analysis between cyanotic versus non-cyanotic defects and operated versus non-operated cases. Methods A 10-year data were retrospectively collected from pregnant women with CHD and a 1:1 ratio of pregnant women without any heart disease. Adverse fetal and cardiac outcomes were noted in both groups. Statistical significance was set at P<0.05. Results A total of 86 pregnant women with CHD were studied, with atrial septal defects (29.06%) being the most common. Out of 86 participants, 27 (31.39%) had cyanotic CHD. Around 55% of cases were already operated on for their cardiac defects. Among cardiovascular complications, 5.8% suffered from heart failure, 7.0% had pulmonary arterial hypertension, 8.1% presented in New York Heart Association functional class IV, 9.3% had a need for intensive care unit admission, and one experienced maternal mortality. Adverse fetal outcomes, including operative vaginal delivery, mean duration of hospital stay, fetal growth restriction, preterm birth (<37 weeks), low birth weight (<2,500 g), 5-minute APGAR score <7, and neonatal intensive care unit admissions, were significantly higher in women with CHD than in women without heart disease. Conclusion Women with CHD have a higher risk of adverse fetal and cardiac outcomes. The outcome can be improved with proper pre-conceptional optimization of the cardiac condition, good antenatal care, and multidisciplinary team management.

Effects of glenohumeral corticosteroid injection on stiffness following arthroscopic rotator cuff repair: a prospective, multicentric, case-control study with 18-month follow-up

Amyn M. Rajani,Urvil A Shah,Anmol RS Mittal,Sheetal Gupta,Rajesh Garg,Alisha A. Rajani,Gautam Shetty,Meenakshi Punamiya,Richa Singhal 대한견주관절학회 2023 대한견주관절의학회지 Vol.26 No.1

Background: This study aimed to analyze the efficacy of single-dose corticosteroid injection (CSI) administered at 6 weeks postoperative to treat stiffness following arthroscopic rotator cuff repair (ARCR). Methods: In this prospective, multicentric, case-control study, post-ARCR stiffness at 6 weeks was treated with either a single dose of intra-articular CSI (CSI group) or physical therapy with oral analgesics (non-CSI group). Pain intensity according to visual analog scale (VAS), functional outcome using the Constant Murley Shoulder Score, time to return to activities of daily living (ADLs), and retear rate were recorded at 6 weeks, 9 weeks, 12 weeks, 6 months, 12 months, and 18 months postoperatively in both groups. Results: A total of 149 patients (54.5%) in the CSI group and 124 patients (45.5%) in the non-CSI group were included in this study. Pain and function were significantly better in the CSI group at 9-week, 12-week, and 6-month (P<0.001) follow-up, whereas they were not significantly different when the groups were compared at 12- and 18-month follow-up. The mean duration to return to ADLs was significantly shorter (P<0.001) in the CSI group. The incidence of retears was not significantly different (P=0.36) between groups at the end of 18 months of follow-up. Conclusions: Single-dose intra-articular CSI administered at 6 weeks postoperative to treat post-ARCR stiffness significantly improved pain, function, and duration of return to ADLs without increasing the risk of retears compared to patients who did not receive intra-articular CSI.