Comprehensive analysis of miRNAs, lncRNAs and mRNAs profiles in backfat tissue between Daweizi and Yorkshire pigs

Chen Chen,Yitong Chang,Yuan Deng,Qingming Cui,Yingying Liu,Huali Li,Huibo Ren,Ji Zhu,Qi Liu,Yinglin Peng Asian Australasian Association of Animal Productio 2023 Animal Bioscience Vol.36 No.3

Objective: Daweizi (DWZ) is a famous indigenous pig breed in China and characterized by tender meat and high fat percentage. However, the expression profiles and functions of transcripts in DWZ pigs is still in infancy. The object of this study was to depict the transcript profiles in DWZ pigs and screen the potential pathway influence adipogenesis and fat deposition, Methods: Histological analysis of backfat tissue was firstly performed between DWZ and lean-type Yorkshire pigs, and then RNA sequencing technology was utilized to explore miRNAs, lncRNAs and mRNAs profiles in backfat tissue. 18 differentially expressed (DE) transcripts were randomly selected for quantitative real-time polymerase chain reaction (QPCR) to validate the reliability of the sequencing results. Finally, gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis were conducted to investigate the potential pathways influence adipocyte differentiation, adipogenesis and lipid metabolism, and a schematic model was further proposed. Results: A total of 1,625 differentially expressed transcripts were identified in DWZ pigs, including 27 upregulated and 45 downregulated miRNAs, 64 upregulated and 119 down-regulated lncRNA, 814 upregulated and 556 downregulated mRNAs. QPCR analysis exhibited strong consistency with the sequencing data. GO and KEGG analysis elucidated that the differentially expressed transcripts were mainly associated with cell growth and death, signal transduction, peroxisome proliferator-activated receptors (PPAR), AMP-activated protein kinase (AMPK), PI3K-Akt, adipocytokine and foxo signaling pathways, all of which are strongly involved in cell development, lipid metabolism and adipogenesis. Further analysis indicated that the BGIR9823_87926/miR-194a-5p/AQP7 network may be effective in the process of adipocyte differentiation or adipogenesis. Conclusion: Our study provides comprehensive insights into the regulatory network of backfat deposition and lipid metabolism in pigs from the point of view of miRNAs, lncRNAs and mRNAs.

AtHAP3b Plays a Crucial Role in the Regulation of Flowering Time in Arabidopsis during Osmotic Stress

Chen, Nai-Zhi,Zhang, Xiu-Qing,Wei, Peng-Cheng,Chen, Qi-Jun,Ren, Fei,Chen, Jia,Wang, Xue-Chen Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.6

The HAP complex has been found in many eukaryotic organisms. HAP recognizes the CCAAT box present in the promoters of 30% of all eukaryotic genes. The HAP complex consists of three subunits - HAP2, HAP3 and HAP5. In this paper, we report the biological function of the AtHAP3b gene that encodes one of the HAP3 subunits in Arabidopsis. Compared with wild-type plants, hap3b-1 and hap3b-2 mutants exhibited a delayed flowering time under long-day photoperiod conditions. Moreover, the transcription levels of FT were substantially lower in the mutants than in the wild-type plants. These results imply that AtHAP3b may function in the control of flowering time by regulating the expression of FT in Arabidopsis. In a subsequent study, AtHAP3b was found to be induced by osmotic stress. Under osmotic stress conditions, the hap3b- 1 and hap3b-2 mutants flowered considerably later than the wild-type plants. These results suggest that the AtHAP3b gene plays more important roles in the control of flowering under osmotic stress in Arabidopsis.

AtHAP3b Plays a Crucial Role in the Regulation of Flowering Time in Arabidopsis during Osmotic Stress

Nai Zhi Chen,Xiu Qing Zhang,Peng Cheng Wei,Qi Jun Chen,Fei Ren,Jia Chen,Xue Chen Wang 한국생화학분자생물학회 (구 한국생화학회) 2007 BMB Reports Vol.40 No.6

Knockdown of HMGN5 Expression by RNA Interference Induces Cell Cycle Arrest in Human Lung Cancer Cells

Chen, Peng,Wang, Xiu-Li,Ma, Zhong-Sen,Xu, Zhong,Jia, Bo,Ren, Jin,Hu, Yu-Xin,Zhang, Qing-Hua,Ma, Tian-Gang,Yan, Bing-Di,Yan, Qing-Zhu,Li, Yan-Lei,Li, Zhen,Yu, Jin-Yan,Gao, Rong,Fan, Na,Li, Bo,Yang, Jun Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

HMGN5 is a typical member of the HMGN (high mobility group nucleosome-binding protein) family which may function as a nucleosomal binding and transcriptional activating protein. Overexpression of HMGN5 has been observed in several human tumors but its role in tumorigenesis has not been fully clarified. To investigate its significance for human lung cancer progression, we successfully constructed a shRNA expression lentiviral vector in which sense and antisense sequences targeting the human HMGN5 were linked with a 9-nucleotide loop. Inhibitory effects of siRNA on endogenous HMGN5 gene expression and protein synthesis were demonstrated via real-time RT-PCR and western blotting. We found HMGN5 silencing to significantly inhibit A549 and H1299 cell proliferation assessed by MTT, BrdU incorporation and colony formation assays. Furthermore, flow cytometry analysis showed that specific knockdown of HMGN5 slowed down the cell cycle at the G0/G1 phase and decreased the populations of A549 and H1299 cells at the S and G2/M phases. Taken together, these results suggest that HMGN5 is directly involved in regulation cell proliferation in A549 and H1299 cells by influencing signaling pathways involved in cell cycle progression. Thus, our finding suggests that targeting HMGN5 may be an effective strategy for human lung cancer treatment.

SLC39A10 promotes malignant phenotypes of gastric cancer cells by activating the CK2-mediated MAPK/ERK and PI3K/AKT pathways

Ren Xiaojuan,Feng Chao,Wang Yubo,Chen Pu,Wang Simeng,Wang Jianling,Cao Hongxin,Li Yujun,Ji Meiju,Hou Peng 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Solute carrier family 39 member 10 (SLC39A10) belongs to a subfamily of zinc transporters and plays a key role in B-cell development. Previous studies have reported that its upregulation promotes breast cancer metastasis by enhancing the influx of zinc ions (Zn2+); however, its role in gastric cancer remains totally unclear. Here, we found that SLC39A10 expression was frequently increased in gastric adenocarcinomas and that SLC39A10 upregulation was strongly associated with poor patient outcomes; in addition, we identified SLC39A10 as a direct target of c-Myc. Functional studies showed that ectopic expression of SLC39A10 in gastric cancer cells dramatically enhanced the proliferation, colony formation, invasiveness abilities of these gastric cancer cells and tumorigenic potential in nude mice. Conversely, SLC39A10 knockdown inhibited gastric cancer cell proliferation and colony formation. Mechanistically, SLC39A10 exerted its carcinogenic effects by increasing Zn2+ availability and subsequently enhancing the enzyme activity of CK2 (casein kinase 2). As a result, the MAPK/ERK and PI3K/AKT pathways, two major downstream effectors of CK2, were activated, while c-Myc, a downstream target of these two pathways, formed a vicious feedback loop with SLC39A10 to drive the malignant progression of gastric cancer. Taken together, our data demonstrate that SLC39A10 is a functional oncogene in gastric cancer and suggest that targeting CK2 is an alternative therapeutic strategy for gastric cancer patients with high SLC39A10 expression.

Integrated Guidance and Control Design based on a Reference Model

Jian Chen,Qingdong Li,Cunjia Liu,Peng Li,Zhang Ren 제어·로봇·시스템학회 2016 International Journal of Control, Automation, and Vol.14 No.5

In this paper, an Integrated Guidance and Control (IGC) algorithm based on a reference model is proposedfor a side-jet missile. First, a IGC structure is introduced, incorporating the response characteristics of the missilecontrol loop into the guidance loop. To describe the response characteristics, then a reference model is built. Next,with the back stepping scheme and sliding mode control algorithm, the reference model is adopted to derive a novelguidance law, which contains response parameters of missile control system to formulate the IGC design. Finally,simulations and comparisons with the time-scale separation design and an existing IGC design, are conducted toverify the proposed IGC algorithm. It can be shown that the proposed algorithm performs better against highlymaneuvering target in different missile-target initial position and heading scenarios.

A Coaxial and Off-axial Integrated Three-mirror Optical System with High Resolution and Large Field of View

Zhe Chen,Junqing Zhu,Jiantao Peng,Xingxiang Zhang,Jianyue Ren 한국광학회 2016 Current Optics and Photonics Vol.20 No.1

A novel optical design for high resolution, large field of view (FOV) and multispectral remote sensingis presented. An f/7.3 Korsch and two f/17.9 Cook three-mirror optical systems are integrated by sharingthe primary and secondary mirrors, bias of the FOV, decentering of the apertures and reasonable structurearrangement. The aperture stop of the Korsch system is located on the primary mirror, while those ofthe Cook systems are on the exit pupils. High resolution image with spectral coverage from visible tonear-infrared (NIR) can be acquired through the Korsch system with a focal length of 14 m, whilewide-field imaging is accomplished by the two Cook systems whose focal lengths are both 13.24 m. The full FOV is 4°×0.13°, a coverage width of 34.9 km at the altitude of 500 km can then be acquiredby push-broom imaging. To facilitate controlling the stray light, the intermediate images and the real exitpupils are spatially available. After optimization, a near diffraction-limited performance and a compactoptical package are achieved. The sharing of the on-axis primary and secondary mirrors reduces the costof fabrication, test, and manufacture effectively. Besides, the two tertiary mirrors of the Cook systemspossess the same parameters, further cutting down the cost.

miR-29a suppresses growth and invasion of gastric cancer cells in vitro by targeting VEGF-A

Ling Chen,Hong Xiao,Zong Hua Wang,Yi Huang,Zi Peng Liu,Hui Ren,Hang Song 생화학분자생물학회 2014 BMB Reports Vol.47 No.1

Stochastic modelling and optimum inspection and maintenance strategy for fatigue affected steel bridge members

Hua-Peng Chen,Tian-Li Huang,Hao Zhou,Wei-Xin Ren 국제구조공학회 2016 Smart Structures and Systems, An International Jou Vol.18 No.3

This paper presents a method for stochastic modelling of fatigue crack growth and optimising inspection and maintenance strategy for the structural members of steel bridges. The fatigue crack evolution is considered as a stochastic process with uncertainties, and the Gamma process is adopted to simulate the propagation of fatigue crack in steel bridge members. From the stochastic modelling for fatigue crack growth, the probability of failure caused by fatigue is predicted over the service life of steel bridge members. The remaining fatigue life of steel bridge members is determined by comparing the fatigue crack length with its predetermined threshold. Furthermore, the probability of detection is adopted to consider the uncertainties in detecting fatigue crack by using existing damage detection techniques. A multi-objective optimisation problem is proposed and solved by a genetic algorithm to determine the optimised inspection and maintenance strategy for the fatigue affected steel bridge members. The optimised strategy is achieved by minimizing the life-cycle cost, including the inspection, maintenance and failure costs, and maximizing the service life after necessary intervention. The number of intervention during the service life is also taken into account to investigate the relationship between the service life and the cost for maintenance. The results from numerical examples show that the proposed method can provide a useful approach for cost-effective inspection and maintenance strategy for fatigue affected steel bridges.

Ultrasmall iron oxide nanoparticles induced ferroptosis via Beclin1/ATG5-dependent autophagy pathway

Wen Jian,Chen Hanren,Ren Zhongyu,Zhang Peng,Chen Jianjiao,Jiang Shulian 나노기술연구협의회 2021 Nano Convergence Vol.8 No.10

Iron-based nanoparticles, which could elicit ferroptosis, is becoming a promising new way to inhibit tumor cell growth. Notably, ultrasmall iron oxide nanoparticles (USIONPs) have been found to upregulate the autophagy process in glioblastoma (GBM) cells. Whether USIONPs could also elicit ferroptosis and the relationship between the USIONPs-induced autophagy and ferroptosis need to be explored. In the current study, our synthesized USIONPs with good water solubility could significantly upregulate the ferroptosis markers in GBM cells, and downregulate the expression of anti-ferroptosis genes. Interestingly,ferrostatin-1 could reverse USIONPs- induced ferroptosis, but inhibitors of apoptosis, pyroptosis, or necrosis could not. Meanwhile, autophagy inhibitor 3-methyladenine could also reverse the USIONPs-induced ferroptosis. In addition, shRNA silencing of upstream genes Beclin1/ATG5 of autophagy process could significantly reverse USIONPs-induced ferroptosis, whereas overexpression of Beclin1/ATG5 of autophagy process could remarkably promote USIONPs-induced ferroptosis. Furthermore, lysosome inhibitors could significantly reverse the USIONPs-induced ferroptosis. Collectively, these facts suggest that USIONPs-induced ferroptosis is regulated via Beclin1/ATG5-dependent autophagy pathway.