Cell Growth Inhibition and Induction of Apoptosis by Snake Venom Toxin in Ovarian Cancer Cell via Inactivation of Nuclear Factor κB and Signal Transducer and Activator of Transcription 3

Ju Kyoung Song,Jin Tae Hong,Mi Ran Jo,Mi Hee Park,Ho Sueb Song,Byeong Jun An,Min Jong Song,Sang Bae Han 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.5

Snake venom toxin from Vipera lebetina turanica induces apoptosis in many cancer cell lines, but there is no study about the apoptotic effect of snake venom toxin on human ovarian cancer cells. In this study, we investigated the apoptotic effect of snake venom toxin in human ovarian cancer PA-1 and SK-OV3 cells. Snake venom toxin dose dependently (0~10 μg/mL) inhibited ovarian cancer cell growth with IC50 values 4.5 μg/mL in PA-1 cells, and 6.5 μg/mL in SKOV3 cells. Our results also showed that apoptotic cell death increased by snake venom toxin in a dose dependent manner (0~10 μg/mL). Consistent with increased cell death, snake venom toxin increased the expression of pro-apoptotic protein Bax and caspase-3, but down-regulated anti-apoptotic protein Bcl-2. Untreated ovarian cancer cells showed a high DNA binding activity of nuclear factor B (NF-κB), but it was inhibited by snake venom toxin accompanied by inhibition of p50 and p65 translocation into the nucleus as well as phosphorylation of inhibitory κB. Snake venom toxin also inhibited DNA binding activity of the signal transducer and activator of transcription 3 (STAT3). Moreover, the combination treatment of NF-κB (salicylic acid, 1 or 5 μM) and STAT3 (stattic, 1 μM) with snake venom toxin (1 μg/mL) further enhanced cell growth inhibitory effects of snake venom toxin. These results showed that snake venom toxin from Vipera lebetina turanica caused apoptotic cell death of ovarian cancer cells through the inhibition of NF-κB and STAT3 signal, and suggested that snake venom toxin may be applicable as an anticancer agent for ovarian cancer.

5-Lipoxygenase inhibitors suppress RANKL-induced osteoclast formation via NFATcl expression

( Ju Hee Kang ),( Zheng Ting ),( Mi Ran Moon ),( Jung Seon Sim ),( Jung Min Lee ),( Kyung Eun Doh ),( Sunhye Hong ),( Minghua Cui ),( Sun Choi ),( Hyeun Wook Chang ),( Hea Young Park Choo ),( Mijung Y 영남대학교 약품개발연구소 2016 영남대학교 약품개발연구소 연구업적집 Vol.26 No.-

5-Lipoxygenase synthesizes leukotrienes from arachidonic acid. We developed three novel 5-LO inhibi-tors having a benzoxazole scaffold as a potential anti-osteoclastogenics. They significantly suppressed RANKL-induced osteoclast formation in mouse bone marrow-derived macrophages. Furthermore, one compound. K7, inhibited the bone resorptive activity of osteoclasts. The anti-osteoclastogenic effect of K7 was mainly attributable to reduction in the expression of NFATc1. an essential transcription factor for osteoclast differentiation. K7 inhibited osteoclast formation via ERK and p38 MAPK. as well as NF-KB signaling pathways. K7 reduced lipopolysaccharide (LPS)-induced osteoclast formation in vivo. corroborating the in vitro data. Thus, IG exerted an inhibitory effect on osteoclast formation in vitro and in vivo, properties that make it a potential candidate for the treatment of bone diseases associated with excessive bone resorption.

Meniscal Ossicle

Ju Hong Lee(이주홍),Sung Il Wang(왕성일),Young Ran Jung(정영란) 대한정형외과학회 2016 대한정형외과학회지 Vol.51 No.2

슬관절의 반월상 연골내 소골은 반월상 연골조직 내에 발생한 골조직으로, 사람에게서는 매우 드문 것으로 보고되고 있다. 저자들은 특별한 외상의 병력 없이 기립 시 발생하는 슬관절통과 간헐적인 잠김증상을 보이는 48세 남자에서 관절경상 내측 반월상 연골 후방 경골 부착부 파열을 동반한 반월상 연골내 소골과 대퇴내과 연골손상으로 관절경적 절제술 및 미세천공술을 시행한 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Meniscal ossicle of the knee, an ossified tissue formed within the meniscus, is rare in humans. We experienced a case of a 48-yearold male with no history of trauma, who presented with intermittent locking symptoms and knee pain upon standing. The patient was diagnosed with meniscal ossicle and cartilage lesion in the medial femoral condyle accompanied by a medial meniscus posterior horn radial tear and treated with arthroscopic resection and microfracture. Therefore, we report on this case with relevant literature.

Simultaneous determination of four azo-dyes by high-performance liquid chromatography-diode array detection and thin layer chromatography

Ju-Hee Park,Joo-Won Lim,Woo-Ri Kim,Seung-Ran Hong,Chanvorleak Phat,Chan Lee 한국식품영양과학회 2016 한국식품영양과학회 학술대회발표집 Vol.2016 No.10

Clinical Microbiology and Biomedical Sciences : Comparative Proteomic Analyses of Synovial Fluids and Serums from Rheumatoid Arthritis Patients

( Ran Noh ),( Sung Goo Park ),( Ji Hyeon Ju ),( Seung Wook Chi ),( Sun Hong Kim ),( Chong Kil Lee ),( Jeong Hoon Kim ),( Byoung Chul Park ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.1

Rheumatoid arthritis (RA) is a chronic and systemic inflammatory disorder that primarily affects the flexible joints and may also affect a number of tissues and organs. The progression of RA involves an inflammatory response of the capsule around the joint, swelling of synovial cells with excess synovial fluid (SF), and the development of fibrous tissue in the synovium. Since the progressive pathology of the disease often leads to the irreversible destruction of articular cartilage and ankylosis of the joint, early diagnosis of RA is essential. Thus, we undertook a comparative proteomic approach to investigate novel biomarkers for early diagnosis using SFs and serums from RA patients. As a result, we identified 32 differentially expressed spots in SFs and 34 spots in serums. The differential expression of the STEAP4 and ZNF 658 proteins were validated using immunoblotting of the SFs and serums, respectively. These data suggest that differentially expressed proteins in SFs and serums could be used as RA-specific biomarkers for the diagnosis and monitoring of RA. Furthermore, these findings advance our understanding of the molecular etiopathogenesis of RA.

Identification of pollutant sources using water quality and stable isotope ratios of inflow tributaries in the lower reaches of the Han-River

Hong, Jung-Ki,Lee, Bo-Mi,Son, Ju Yeon,Park, Jin-Rak,Lee, Sung Hye,Kim, Kap-Soon,Yu, Soon-Ju,Noh, Hye-ran The Korean Society of Analytical Science 2019 분석과학 Vol.32 No.2

Despite the expansion of sewage treatment facilities to reduce pollutants in the tributaries of the Han River, water pollution accidents such as fish deaths continue to frequently occur. The purpose of this study was to identify the pollutant sources using water quality and stable isotope ratio (${\delta}^{15}N$, ${\delta}^{13}C$, ${\delta}^{15}N-NH_4$, ${\delta}^{15}N-NO_3$) analysis results in the three inflow tributaries (Gulpocheon (GP), Anyangcheon (AY) and Sincheon (SC)) of the Han River. Water quality was analyzed in June and October from 2013 to 2017, and the results showed that the concentrations of nutrients, such as T-N, $NO_3-N$, and T-P, were increased at GP4, AY3, SC3, and SC4, which lie downstream of sewage treatment facilities. The results of ${\delta}^{15}N$ for June 2017 indicated that the source of nitrogen was sewage or livestock excreta at GP4 and SC4, and organic fertilizers at AY3 and SC3. ${\delta}^{15}N-NO_3$ results suggested that the source of nitrogen was related to organic sewage, livestock or manure at GP4, AY3 and SC4. Therefore, GP4 and SC4 were more influenced by effluent from sewage treatment facilities than by their tributaries, AY3 and SC3 were considered to be influenced more by their tributary than effluent from sewage treatment facilities. With the results of this study, the source of contamination (sewage treatment facility effluent) of river inflow downstream of Han River could be confirmed using water quality and stable isotope ratio.

Residential green environments and pregnancy outcomes: systemic review and meta-analysis

( Kyung Ju Lee ),( Hyemi Moon ),( Hyo Ri Yun ),( Eun Lyeong Park ),( Ae Ran Park ),( Juneyoung Lee ),( Ki Hoon Ahn ),( Soon Cheol Hong ),( Min Jeong Oh ),( Hai Joong Kim ) 대한산부인과학회 2019 대한산부인과학회 학술대회 Vol.105 No.-

Objective: In modern society people live in large cities is important to consider the social needs of site selection and design for green spaces related with planning for environmental medicine and public health. Although the majority of published studies investigated health effect related to the cumulative greenery and air pollutions, but recently the public health community started to take a closer look at the associations between residential greenery and fetal development and pregnancy outcomes. In utero exposures to prenatal environment with air pollution exposure and greenery is an important period of susceptibility to health effects including risk of low birth weight, preterm birth, and preeclampsia. However, the modern urban-ecological systems associated with prenatal environment is limited. This study was aimed the answer the question whether living environment including green spaces and air pollutions during pregnancy are associated with adverse pregnancy outcome and what the direction of that effect is, and to propose some new methodological approaches. Methods: MEDLINE, EMBASE, Cochrane Library, K-eArticles and CINAHL databases were searched for relavant publications in English from inception up to May 2019, based on the following free-text term key- words: population (pregnancy or pregnant women), intervention (green space, parks, forest and greenness), and outcome (pregnancy outcome and birth outcome). Data were analyzed as risk ratios (RR) or odds ratios (OR), and effects were adjusted for clustering. Meta-analyses were performed using random-effects and evidence quality was assessed. For the quality assessment, Risk of Bias Assessment tool for Non-randomized Studies for observational studies were used. Results: Out of 89 records, ten studies were identified and included in the analyses. We report quality effects meta-analyses based on correlation and standardized regression coefficients as estimates of effect size. Living greenness environment within 100-m, 250m, 500m buffer was weakly and positively associated with birth weight even though air quality and civilization considered. Heterogeneity was high (I 2 = 88%) and under the quality effects model the meta-analysis produced significant pooled regression slope of 0.0016(95%CI: 0.0007, 0.0025). Conclusion: In this study even if urbanization intensifies, greenness had positive effect on pregnancy outcomes, and this study endorses the importance of green space management and planning for environmental medicine and public health future research.

High-resolution Impedance Manometry Criteria in the Sitting Position Indicative of Incomplete Bolus Clearance

( Eui Ju Park ),( Joon Seong Lee ),( Tae Hee Lee ),( Gene Hyun Bok ),( Su Jin Hong ),( Hyun Gun Kim ),( Seong Ran Jeon ),( Jin Oh Kim ) 대한소화기기능성질환·운동학회 2014 Journal of Neurogastroenterology and Motility (JNM Vol.20 No.4

Background/Aims We used high-resolution impedance manometry (HRIM) to determine the length of breaks on the isobaric contour which would predict incomplete bolus clearance in patients in the sitting position. Methods In total, 651 swallows in 71 patients with esophageal symptoms were studied using a solid-state HRIM system in the sitting position. Each swallow was classified as complete or incomplete bolus clearance by impedance criteria and peristaltic integrity was evaluated using 20- and 30-mmHg pressure topography isobaric contours. Correlations between the lengths of the breaks for 20- and 30-mmHg were analyzed. Results Complete bolus clearance was observed in 83.3% (542/651) of swallows. Breaks of 3 and 7 cm or less were associated with a bolus clearance of 96.8% on the 20-mmHg and 94.7% on the 30-mmHg isobaric contour, respectively (P < 0.001). The areas under the ROC curves for the 20 and 30 mmHg isobaric contours were 0.900 and 0.950, respectively. The sensitivity and specificity for complete bolus clearance were 75.6% and 89.3% for breaks 3 cm or less on the 20-mmHg isobaric contour and 87.9% and 78.7% for breaks 7 cm or less on the 30-mmHg contour (P < 0.001). Conclusions Breaks of < 3 cm in the 20-mmHg isobaric contour or < 7 cm in the 30-mmHg isobaric contour were associated with com - plete bolus clearance. The threshold for breaks in the sitting position was greater than previous reports using the supine position and longer breaks predicted incomplete bolus clearance.

지중해의 오염규제를 위한 국제협약에 관한 연구

홍란주 ( Ran Ju Hong ),박명섭 ( Myong Sop Pak ),한능호 ( Neung Ho Han ) 韓國海法學會 2009 韓國海法學會誌 Vol.31 No.1

해운은 오랜 기간 동안 안전하고 환경친화적인 형태로 이루어져 왔으며 그 역할을 위한 운송방법 또한 지속적으로 개선되어 왔다. 그러나 운송이 늘어나면서 선박사고로 인한 유출사례가 증가하고, 환경보호가 글로벌 이슈화됨에 따라 대다수의 국가와 국제기구에서는 환경법안 제정을 통해 해양 오염규제를 강화하고 있다. 특히 지중해는 다른 해역으로 향하는 출구가 뚫린 반폐쇄해성 수역으로 해수순환이 상당히 제한적이고 전세계 물량의 90%가 이송되는 주요 해운로로서 대부분의 선박은 원유이송 및 급유와 무역에 따른 물품이송을 목적으로 지중해를 통행하고 있다. 지중해의 이러한 지리적 특성과 높은 통행량은 해상사고 및 지중해 오염의 위험도를 높이고 있으며, 이에 따라 지중해 연안국가들은 해양오염 규제를 위해 공동으로 노력하고 있다. 해상사고나 투기를 야기하는 선박기인 해양오염에 대한 규제는 IMO와 UNCLOS의 협약 및 조약에 의해 이루어지고 있는데 그 중에서도 특히 MARPOL 73/78을 근간으로 UNEP의 지역해 프로그램이 주목할 만하다. 따라서 본 논문에서는 해양오염 규제를 위한 국제협약과 조약을 바탕으로 지중해 지역에 적용되고 있는 협약을 살펴보고 연안국가들의 동향과 지중해 행동계획을 포함한 오염 규제안에 대한 구체적인 내용을 다루고자 한다. Shipping has always been a safe, secure and environmentally friendly form of transport and its performance has improved up to now. Due to the growth of sea transport, the oil pollution damage has been increased. As environmental protection of the earth has become the essential issue, various nations and international organizations have strengthen the pollution prevention with environment legislations. The Mediterranean is especially the semi-enclosed sea and the seawater circulation is very restrictive. Also it is a vital artery for merchant shipping, which transports 90 per cent of the world`s goods. The vast majority of oil traffic transits and trade volume are completed safely, efficiently and without harm to the marine environment. The Mediterranean`s geographical peculiarities and high traffic raise the danger of pollution and the coastal countries make an collective effort for the pollution prevention. The prevention for the vessel source pollution is formed by IMO and UNCLOS. IMO`s work to prevent and reduce marine pollution by ships has made it a natural partner for UNEP in protecting the world`s oceans in the context of the Regional Seas Programme. The purpose of this study is to examine international agreements for maritime pollution prevention and the international agreements which can be applied to the Mediterranean region. Through looking into the agreements and protocols, we investigate the movement of Mediterranean coastal countries to protect their sea also review their operation.

선박기인 대기오염 감축을 위한 배출규제해역과 주변국의 규제에 관한 연구

홍란주(Ran-Ju Hong),허윤석(Yun-Seok Hur) 한국해양비즈니스학회 2014 해양비즈니스 Vol.- No.27

Air pollution from shipping has been increasing over the last 10 years and today's shipping is under increasing pressure to comply with evolving regulations to become cleaner and greener. The main regulation behind these changes is the IMO's MARPOL Annex VI which sets out substantial changes in exhaust emission controls and designates Emission Control Areas(ECAs) for NOX, SOX and particulate matter. The increased interest in emission policies and regulations has created an explosion of International, EU, U.S. and Canada concerning with ECAs. This study provides an overview of international framework, as the ECAs, for regulating air emissions from ships, and marginal states' policies and regulations on air emissions from international shipping. The main purpose of this study is to examine the ECAs and the marginal states' regulations for reducing the air emissions from ships, and to suggest some implications for non-ECAs, especially Korea.