Neuroprotective and Neurorestorative Role of Lactucopicrin in Neuronal via Ca <SUP>2+</SUP> / CaMKII / ATF1 Neuritogenesis and trophic Factor Mediated potentiation in Non-Neuronal cells

Ramu VENKATESAN,Hussain Mushab WAHEDI,Doo oh MOON,Sun Yeou KIM 한국생물공학회 2015 한국생물공학회 학술대회 Vol.2015 No.4

Response of rigid footing on reinforced granular fill over soft soil

Ramu, K.,Madhav, Madhira R. Techno-Press 2010 Geomechanics & engineering Vol.2 No.4

An extended model for the response of a rigid footing on a reinforced foundation bed on super soft soil is proposed by incorporating the rough membrane element into the granular bed. The super soft soil, the granular bed and the reinforcement are modeled as non-linear Winkler springs, non-linear Pasternak layer and rough membrane respectively. The hyperbolic stress-displacement response of the super soft soil and the hyperbolic shear stress-shear strain response of the granular fill are considered. The finite deformation theory is used since large settlements are expected to develop due to deformation of the super-soft soil. Parametric studies quantify the effect of each parameter on the stress-settlement response of the reinforced foundation bed, the settlement and tension profiles.

Low temperature synthesis of Na_0.33V₂O₅ and their Li⁺ ion supercapacitive behavior

( Ramu Manikandan ),( Murugesan Rajesh ),박승일,( C. Justin Raj ),김병철,유국현 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.1

The sodium vanadium oxide exists in several forms NaV₆O₁₅, NaVO₂, NaVO₃ and NaV₃O₈. In this present work, we prepared Na_0.33V₂O₅ nanostructure by simple co-precipitation technique at low temperature (80℃) using VOSO_4.xH₂O and NaOH solution. The structural and morphological properties of Na_0.33V₂O₅ were studied using XRD, Raman spectrum and SEM analysis. The Li+ ion supercapacitive behavior of the Na_0.33V₂O₅ electrode was investigated by means of CV, GCD technique and EIS in 1M LiClO₄ electrolyte. The Na_0.33V₂O₅ electrode showed maximum capacitance of 164 F g^-1 at 1A g^-1.

Efficient genome editing by FACS enrichment of paired D10ACas9 nickases coupled with fluorescent proteins

Ramu Gopalappa,송명재,아룬판디안찬드라세카란,Soumyadip Das,sabahaq,고현철,Suresh Ramakrishna 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.9

Targeted genome editing by clustered regularly interspaced short palindromic repeats (CRISPR-Cas9) raised concerns over off-target effects. The use of doublenicking strategy using paired Cas9 nickase has been developed to minimize off-target effects. However, it was reported that the efficiency of paired nickases were comparable or lower than that of either corresponding nuclease alone. Recently, we conducted a systematic comparison of the efficiencies of several paired Cas9 with their corresponding Cas9 nucleases and showed that paired D10A Cas9 nickases are sometimes more efficient than individual nucleases for gene disruption. However, sometimes the designed paired Cas9 nickases exhibited significantly lower mutation frequencies than nucleases, hampering the generation of cells containing paired Cas9 nickase-induced mutations. Here we implemented IRES peptide-conjugation of fluorescent protein to Cas9 nickase and subjected for fluorescence-activated cell sorting. The sorted cell populations are highly enriched with cells containing paired Cas9 nickase-induced mutations, by a factor of up to 40-fold as compared with the unsorted population. Furthermore, gene-disrupted single cell clones using paired nickases followed by FACS sorting strategy were generated highly efficiently, without compromising with its low off-target effects. We envision that our fluorescent protein coupled paired nickase-mediated gene disruption, facilitating efficient and highly specific genome editing in medical research.

Nondierentiable multiobjective higher-order duality relations for unified type dual models under type-I functions

Ramu Dubey,Lakshmi Narayan Mishra 장전수학회 2019 Advanced Studies in Contemporary Mathematics Vol.29 No.3

The motivation behind this paper is to study a class of nondierentiable multiobjective fractional program- ming problem in which each component of objective functions contains a term including the support function of a compact convex set. For a dierentiable function, we consider the class of second-order (F; ; ; d)-type- I convex functions. Further, we formulate unied (mixed type) dual models and derived duality relations under aforesaid assumptions.

1P-375 Na_xV₂O₅ Nano Composite for High-Performance Symmetric Pseudocapacitor

( Ramu Manikandan ),박승일,( Murugesan Rajesh ),유국현 한국공업화학회 2017 한국공업화학회 연구논문 초록집 Vol.2017 No.1

Symmetric supercapacitor was fabricated using NaxV₂O₅ nanocomposite synthesized via a conventional co-precipitation technique. The structural and morphological investigation showed that the synthesized Na_xV₂O₅ sample exhibited good crystallinity with nanorod stacked morphology. The electrochemical properties of the Na_xV₂O₅ symmetric supercapacitor was performed in 1M Na₂SO₄ aqueous electrolyte. The fabricated Na_xV₂O₅ symmetric supercapacitors exhibited excellent electrochemical capacitance behavior with maximum specific capacitance value of 166 F g^-1 at 0.5 A g^-1. Moreover, Na_0.33V₂O₅ symmetric supercapacitors demonstrated an excellent cyclic stability with a high capacitance retention of ~87% after 50,000 charge/ discharge cycles.

Lactucopicrin potentiates neuritogenesis and neurotrophic effects by regulating Ca²⁺/CaMKII/ATF1 signaling pathway

Venkatesan, Ramu,Shim, Won-Sik,Yeo, Eui-Ju,Kim, Sun Yeou Elsevier Ireland Ltd 2017 Journal of Ethnopharmacology Vol.198 No.-

<P><B>Abstract</B></P> <P><B>Ethno-pharmacological relevance</B></P> <P>Lactucopicrin is one of constitutes in <I>Cichorium intybus L,</I> which is commonly known as chicory in worldwide. It has been used for traditional usage such as antianalgesics, antidepressants and antihyperglycemics</P> <P><B>Aim of study</B></P> <P>We investigated the neurotrophin-mediated neuroprotective effect of lactucopicrin in <I>in vitro</I> and examined for the underlying mechanism.</P> <P><B>Materials and method</B></P> <P>To verify the neuroprotective effect of lactucopicrin, we investigated the inhibitory AChE activity, neurite outgrowth-related downstream signaling in murine neuroblastoma N2a and neurotrophins secretion in rat C6 glioma cells.</P> <P><B>Results</B></P> <P>Lactucopicrin inhibited the AChE activity and increased intracellular Ca<SUP>2+</SUP> levels with a substantial rise in muscarinic acetylcholine receptor M1 (CHRM1) expression in N2a cells. Moreover, lactucopicrin actively promoted neurite outgrowth via Ca<SUP>2+</SUP>-mediated activation of Ca<SUP>2+</SUP>/calmodulin-dependent protein kinase-II (CaMKII). It further activates transcription factor 1 (ATF1) along with modulating the levels of tropomyosin receptor kinase A, extracellular signal-regulated kinase 1 and 2, AKT, and synaptophysin 1 in N2a cells. Additionally, the levels of neurotrophins including NGF, BDNF, and NT3 were increased by treatment of lactucopicrin in C6 cells. The effects of lactucopicrin on NGF secretion and neuritogenesis were maintained even in the presence of phosphatidylinositol-3-kinase (PI3K) inhibitor LY294002, indicating that lactucopicrin exerts its effect on neuritogenesis in a PI3K-independent manner.</P> <P><B>Conclusion</B></P> <P>Our results suggest that the natural compound lactucopicrin may be a promising neurotrophin-mediated neuroprotective candidate for neurodegenerative diseases.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Establishment of Structural Similitude for Elastic Models and Validation of Scaling Laws

Murugan Ramu,V. Prabhu Raja,P. R. Thyla 대한토목학회 2013 KSCE JOURNAL OF CIVIL ENGINEERING Vol.17 No.1

Scaled down models are widely used for experimental investigations on huge structures due to the limitation in the capacities of testing facilities, and, moreover, the experimentation on scaled models is less expensive. Also small scale models are often built with materials dissimilar to those of the prototype. However, only few studies have been carried out on the similitude laws for the prototype structures with small scale models built with a different material. In the present study, an attempt has been made to develop a scaling law for models to carry out the free vibration analysis of structures, based on the similitude requirement. The established scaling laws are validated analytically using simple test problems. The similitude relationship between the prototype and the model is validated with the aid of a case study using finite element analysis software.

Selective design of binder-free hierarchical nickel molybdenum sulfide as a novel battery-type material for hybrid supercapacitors

Manikandan, Ramu,Raj, C. Justin,Nagaraju, Goli,Pyo, Myoungho,Kim, Byung Chul The Royal Society of Chemistry 2019 Journal of Materials Chemistry A Vol.7 No.44

<P>Recently, binder-free and hierarchical electrode materials have attracted special attention for the rational design of high-energy density hybrid supercapacitors. Herein, we demonstrated binder-free nickel molybdenum sulfide nano-flakes on nickel foam (NMS-Ni) using a facile successive ionic layer adsorption and reaction (SILAR) process for the fabrication of high-performance hybrid supercapacitors. The selective SILAR cycles had a significant effect on the morphology and electrochemical properties of the NMS nanostructures. Specifically, the NMS deposited for 40 cycles (40cyc@NMS-Ni) displayed the maximum areal capacity (<I>C</I>Ac) of 2.8 C cm<SUP>−2</SUP> (2224 C g<SUP>−1</SUP>) at the current density 4 mA cm<SUP>−2</SUP> in a 6 M KOH electrolyte. Furthermore, a hybrid supercapacitor (HSC) was fabricated using 40cyc@NMS-Ni as the positive electrode and N,O-enriched activated carbon (N,O-AC)-coated Ni-foam as the negative electrode, which showed the maximum potential and specific capacitance (<I>C</I>F-cell) of 1.5 V and 111 F g<SUP>−1</SUP>, respectively. Moreover, the device displayed an outstanding specific energy and specific power of 35 W h kg<SUP>−1</SUP> and 1.5 kW kg<SUP>−1</SUP> with an excellent capacitance retention (95%) after 5000 cycles, respectively. Thus, based on the observed results, it can be concluded that the present study demonstrates a route to utilize NMS-based electrodes as a promising material for high-performance energy storage devices.</P>

Recent Progress on Metal-Organic Framework Membranes for Gas Separations: Conventional Synthesis vs. Microwave-Assisted Synthesis

Gokulakrishnan Ramu,Hae-Kwon Jeong 한국막학회 2017 멤브레인 Vol.27 No.1

금속유기구조체(metal-organic framework)는 유기물와 무기물로 구성된 나노다공성 결정물질로서 일정한 세공 구 조를 가지고 있다. 합성시 유기 리간드의 다양한 선택이 가능함으로 인해 다양한 세공 사이즈와 물리적/화학적 성질들을 나 타내는 금속유기구조체가 가능하다. 이러한 특성들로 인해 다공성 금속유기구조체는 새로운 기체분리용 막 재료로 각광받고 있다. 그러나 고품질의 다결정 금속유기구조체막을 재조하는 것은 상당히 어려운 일인데, 이것은 지지체 표면에 금속유기구 조체 결정을 자라게 하는 것이 쉽지 않기 때문이다. 지난 이십여 년 동안 마이크로 전자파를 이용한 물질 합성에 대한 연구 가 상당히 진행되었는데 특히 마이크로 전자파를 이용하면 전통적인 합성법에 비해 금속유기구조체 분리막을 제조하는 과정 에서의 어려움들을 극복할 수 있다. 마이크로 전자파를 이용한 다결정 분리막 제조 공정은 단시간 합성, 복잡한 구조체 합성 및 나노 결정체 합성 등의 장점이 있다. 본 총설에서는 금속유기구조체 분리막 제조 및 기체 분리에 관한 최근 연구성과들을 살펴보고 특히 마이크로 전자파를 이용한 분리막 제조 공정을 중심으로 정리한다. Metal-organic frameworks (MOFs) are nanoporous materials that consist of organic and inorganic moieties, with well-defined crystalline lattices and pore structures. With a judicious choice of organic linkers present in the MOFs with different sizes and chemical groups, MOFs exhibit a wide variety of pore sizes and chemical/physical properties. This makes MOFs extremely attractive as novel membrane materials for gas separation applications. However, the synthesis of high-quality MOF thin films and membranes is quite challenging due to difficulties in controlling the heterogeneous nucleation/ growth and achieving strong attachment of films on porous supports. Microwave-based synthesis technology has made tremendous progress in the last two decades and has been utilized to overcome some of these challenges associated with MOF membrane fabrication. The advantages of microwaves as opposed to conventional synthesis techniques for MOFs include shorter synthesis times, ability to achieve unique and complex structures and crystal size reductions. Here, we review the recent progress on the synthesis of MOF thin films and membranes with an emphasis on how microwaves have been utilized in the synthesis, improved properties achieved and gas separation performance of these films and membranes.