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RISS 인기검색어
- 검색키워드
- 저자 : Ramazan Beyaz (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
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Beyaz, Ramazan,Darcin, E. Selcen,Aycan, Murat,Kayan, Mustafa,Yildiz, Mustafa The Korean Society of Plant Biotechnology 2016 식물생명공학회지 Vol.43 No.2
In this study, routinely used transformation method, which includes transferring explants onto co-cultivation medium after inoculating them with bacterial solution for a while, was compared with 3 different inoculation methods. In every 3 methods, hypocotyl explants excised from 7-day-old sterile flax seedlings having cotyledon leaves and no root system dried under air flow in sterile cabin for 35 min were inoculated with different volumes of bacterial solution at different inoculation periods. GV2260 line of Agrobacterium tumefaciens having 'pBIN 19' plasmid containing npt II (neomycin phosphotransferase II) gene and GUS reporter gene was used in transformation studies. After inoculation, hypocotyl segments of seedlings (0.5 cm in length) - were excised and left to co-cultivation for 2 days. Then, explants were transferred to regeneration medium supplemented with different antibiotics. The presence of npt-II and GUS genes in transformants was confirmed by PCR and GUS analysis. The highest results in all characters examined in all cultivars were obtained from the 2 inoculation method in which hypocotyls excised from seedlings inoculated with $500{\mu}l$ of bacterial solution after drying in sterile cabin for 35 min were used.
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Ramazan Beyaz,E. Selcen Darçın,Murat Aycan,Mustafa Kayan,Mustafa Yıldız 한국식물생명공학회 2016 JOURNAL OF PLANT BIOTECHNOLOGY Vol.43 No.2
In this study, routinely used transformation method, which includes transferring explants onto co-cultivation medium after inoculating them with bacterial solution for a while, was compared with 3 different inoculation methods. In every 3 methods, hypocotyl explants excised from 7-day-old sterile flax seedlings having cotyledon leaves and no root system dried under air flow in sterile cabin for 35 min were inoculated with different volumes of bacterial solution at different inoculation periods. GV2260 line of Agrobacterium tumefaciens having ‘pBIN 19’ plasmid containing npt II (neomycin phosphotransferase II) gene and GUS reporter gene was used in transformation studies. After inoculation, hypocotyl segments of seedlings (0.5 cm in length) - were excised and left to co-cultivation for 2 days. Then, explants were transferred to regeneration medium supplemented with different antibiotics. The presence of npt-II and GUS genes in transformants was confirmed by PCR and GUS analysis. The highest results in all characters examined in all cultivars were obtained from the 2 inoculation method in which hypocotyls excised from seedlings inoculated with 500 μl of bacterial solution after drying in sterile cabin for 35 min were used.
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Hybrid Magnetic‑Piezoelectric Energy Harvester for Power Generation around Waistline During Gait
Mustafa İlker Beyaz,Fatih Tat,Kamil Yunus Özkaya,Ramazan Özbek 대한전기학회 2020 Journal of Electrical Engineering & Technology Vol.15 No.1
The development of a novel hybrid energy harvester for scavenging power around human waistline is presented. The harvester is composed of mm-scale electromagnetic coils and piezoelectric transducers incorporated on a belt, as well as permanent magnet structures placed on wristbands. The coils and transducers are positioned on the belt such that they correspond to either sides of the waist, facing the wrists and the hipbone. This confguration leads to simultaneous power generation on the coils and transducers due to swinging arm motion and varying belt stress during gait, respectively. Moderate walking at 1 m/s resulted in a peak open circuit voltage of 2.8 V on the coils, reaching up to 4.3 V at 2 m/s fast walking. The open circuit voltage obtained from the transducers varied from 5.2 to 9 V, corresponding to moderate walking at 1 m/s and running at a speed of 3 m/s, respectively. Instantaneous AC power was characterized separately at 1 m/s walking speed, and measured to be 14 mW and 13.5 μW from the coils and piezoelectric transducers, respectively. The two harvesting mechanisms were coupled with rectifers and DC/DC converter circuitry to store energy in an onboard 20 mAh 3 V LiPo battery. Walking for 10 min was shown to raise the battery voltage from 2.2 to 2.8 V. The results reported here demonstrate the feasibility of this MEMS-scale harvesting scheme to recharge batteries of portable electronic devices.
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