Prohibitin 1 Regulates the H19-Igf2 Axis and Proliferation in Hepatocytes

Ramani, Komal,Mavila, Nirmala,Ko, Kwang Suk,Mato, José,M.,Lu, Shelly C. American Society for Biochemistry and Molecular Bi 2016 The Journal of biological chemistry Vol.291 No.46

<P>Prohibitin 1 (PHB1) is a mitochondrial chaperone that regulates cell growth. Phb1 knock-out mice exhibit liver injury and hepatocellular carcinoma (HCC). Phb1 knock-out livers show induction of tumor growth-associated genes, H19 and insulin-like growth factor 2 (Igf2). These genes are controlled by the imprinting control region (ICR) containing CCCTC-binding transcription factor (CTCF)-binding sites. Because Phb1 knock-out mice exhibited induction of H19 and Igf2, we hypothesized that PHB1-mediated regulation of the H19-Igf2 axis might control cell proliferation in normal hepatocytes. H19 and Igf2 were induced (8-20-fold) in 3-week-old Phb1 knock-out livers, in Phb1 siRNA-treated AML12 hepatocytes (2-fold), and HCC cell lines when compared with control. Phb1 knockdown lowered CTCF protein in AML12 by approximate to 30% when compared with control. CTCF overexpression lowered basal H19 and Igf2 expression by 30% and suppressed Phb1 knockdown-mediated induction of these genes. CTCF and PHB1 co-immunoprecipitated and co-localized on the ICR element, and Phb1 knockdown lowered CTCF ICR binding activity. The results suggest that PHB1 and CTCF cooperation may control the H19-Igf2 axis. Human HCC tissues with high levels of H19 and IGF2 exhibited a 40-50% reduction in PHB1 and CTCF expression and their ICR binding activity. Silencing Phb1 or overexpressing H19 in the mouse HCC cell line, SAMe-D, induced cell growth. Blocking H19 induction prevented Phb1 knockdown-mediated growth, whereas H19 overexpression had the reverse effect. Interestingly H19 silencing induced PHB1 expression. Taken together, our results demonstrate that the H19-Igf2 axis is negatively regulated by CTCF-PHB1 cooperation and that H19 is involved in modulating the growth-suppressive effect of PHB1 in the liver.</P>

Event Driven Architectural Style for Customer Centric eGovernment System

Ramani. S,Y. S. Kumaraswamy 보안공학연구지원센터 2014 International Journal of Software Engineering and Vol.8 No.6

A New Symmetric Multilevel Inverter Topology Using Single and Double Source Sub-Multilevel Inverters

Ramani, Kannan,Sathik, Mohd. Ali Jagabar,Sivakumar, Selvam The Korean Institute of Power Electronics 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.1

In recent years, the multilevel converters have been given more attention due to their modularity, reliability, failure management and multi stepped output waveform with less total harmonic distortion. This paper presents a novel symmetric multilevel inverter topology with reduced switching components to generate a high quality stepped sinusoidal voltage waveform. The series and parallel combinations of switches in the proposed topology reduce the total number of conducting switches in each level of output voltages. In addition, a comparison between the proposed topology with another topology from the literature is presented. To verify the proposed topology, the computer based simulation model is developed using MATLAB/Simulink and experimentally with a prototype model results are then compared.

Microstructure Evolution and Shear Strength Study of Sn–9Zn and Sn–8Zn–3Bi on Cu Substrate

Ramani Mayappan,Zainal Arifin Ahmad 한국전기전자재료학회 2024 Transactions on Electrical and Electronic Material Vol.25 No.1

In electronics assembly, solder joints not only serve as mechanical supporters-, but also as heat removers. Therefore, solder joint reliability is a major concern not only during production, but also during the life time of electronic products. It can be pointed out that reliability of solder joint determines the lifetime of electronics products. This issue becomes more important with the use of Pb-free soldering alloys. This study focuses on the reliability of Sn–9Zn and Sn–8Zn–3Bi lead-free solder joints with Cu substrates. This study deals with interface intermetallic morphology, solder joint strength and fracture surface analysis after isothermal aging at 50, 100, and 150°C for 100, 250, and 500 h. For all aging conditions, a flat Cu 5 Zn 8 intermetallic was formed, and as the aging conditions increased, Cu atoms diffused through the intermetallic to form isolated Cu5 Zn8 intermetallics in the bulk solder. Furthermore, Sn atoms from bulk the solder diffused through the intermetallic to form Cu6 Sn5 on the Cu side. The formation of a thick Cu5 Zn8 intermetallic and the diffusion of Sn and Zn atoms created a depletion zone near the solder/intermetallic boundary, which weakened the joint strength. As the intermetallic layer thickness increased, the joint strength decreased as the aging increased. The fracture path generally occurred at the bulk solder/intermetallic boundary under all aging conditions.

Hypertensive Retinopathy and the Risk of Hemorrhagic Stroke

Thiagarajah, Ramani,Kandasamy, Regunath,Sellamuthu, Pulivendhan The Korean Neurosurgical Society 2021 Journal of Korean neurosurgical society Vol.64 No.4

Objective : Hemorrhagic stroke (HS) and hypertensive retinopathy are known end organ damage of the brain and eye respectively, with HS having deleterious consequence to the patients. This study is to correlate between hypertensive retinopathy and HS in hypertensive disease. Methods : A control group of hypertensive patients only, and an investigated group of hypertensive HS patients. Fundoscopic examination to determine the grade of retinopathy was performed and then divided into low or high severity hypertensive retinopathy. Clinical and radiological parameter included are demography, vital signs, Glasgow coma scale (GCS) on admission, clot volume, site of clot, Intracerebral hemorrhage (ICH) score and Glasgow outcome scale (GOS). Data were correlated with the severity of hypertensive retinopathy. Results : Fifty patient in the control group and 51 patients in the investigated group were recruited. In the hypertensive HS group, 21 had low severity retinopathy (no or mild retinopathy) accounting for 41.2% and 30 patients had high severity (moderate or severe retinopathy). In the hypertensive patients 49 had low severity and one had high severity (p-value of 0.001). In HS group low severity showed better GCS score of 9-15 on admission (p-value of 0.003), clot volume less than 30 mL (p-value 0.001), and also a better 30 days mortality rate by using the ICH score (p-value 0.006), GOS score of 4 and 5 the low severity retinopathy fair better than the high severity retinopathy (p-value of 0.001), and the relative risk to develop HS in low severity and high severity retinopathy was 0.42 and 29.4, respectively. Conclusion : Hypertensive retinopathy screening could be used as an indicator in hypertensive patient, to evaluate the risk of developing hypertensive HS in the future.

Study of Conjunctival Microbial Flora in Patients of Intensive Care Unit

Kadambari Ramani,Subashini Kaliaperumal,Sandip Sarkar,Sujatha Sistla 대한안과학회 2021 Korean Journal of Ophthalmology Vol.35 No.4

Purpose: The objective of the study was to evaluate the type of conjunctival microbial flora in intensive care unit patients and their antimicrobial sensitivity pattern. Methods: A total of 272 samples (conjunctival swabs) were taken from patients in various intensive care units and sent for culture and sensitivity. An ocular examination was done to look for lagophthalmos, conjunctival discharge, exposure keratitis, and corneal perforation. Results: Majority (82.1%) of the samples showed at least one microbial isolate while 29 (10.7%) samples showed multiple microbial growth. The most common microbes were coagulase negative Staphylococcus spp. (41.5% of isolates), diphtheroids (11.0% of isolates), and Staphylococcus aureus (9.6% of isolates) which are the usual commensals of the ocular surface. Of the other microbes isolated, Pseudomonas aeruginosa (4.0%) was the most common. Eighty-four percent isolates of coagulase negative Staphylococcus sp., 81.8% isolates of diphtheroids and 100% isolates of Staphylococcus aureus were penicillin resistant. All isolates of Enterococcus fecalis were sensitive only to vancomycin. Two hundred and twenty eyes (80.9%) had varying degrees of lagophthalmos. Nineteen (7.0%) had severe corneal exposure changes leading to infectious corneal ulcer and perforation in all of them. Conclusions: The isolates in patients of intensive care units were no different from the normal conjunctival flora though few pathogenic organisms such as Pseudomonas aeruginosa and Acinetobacter sp. were also isolated. Most of the isolates were penicillin resistant. This knowledge will help take appropriate prophylactic measures to contain ocular infections in the intensive care units.

Technical requirements for cultured meat production: a review

( Sivasubramanian Ramani ),( Deunsol Ko ),( Bosung Kim ),( Changjun Cho ),( Woosang Kim ),( Cheorun Jo ),( Chang-Kyu Lee ),( Jungsun Kang ),( Sunjin Hur ),( Sungkwon Park ) 한국축산학회 2021 한국축산학회지 Vol.63 No.4

Environment, food, and disease have a selective force on the present and future as well as our genome. Adaptation of livestock and the environmental nexus, including forest encroachment for anthropological needs, has been proven to cause emerging infectious diseases. Further, these demand changes in meat production and market systems. Meat is a reliable source of protein, with a majority of the world population consumes meat. To meet the increasing demands of meat production as well as address issues, such as current environmental pollution, animal welfare, and outbreaks, cellular agriculture has emerged as one of the next industrial revolutions. Lab grown meat or cell cultured meat is a promising way to pursue this; however, it still needs to resemble traditional meat and be assured safety for human consumption. Further, to mimic the palatability of traditional meat, the process of cultured meat production starts from skeletal muscle progenitor cells isolated from animals that proliferate and differentiate into skeletal muscle using cell culture techniques. Due to several lacunae in the current approaches, production of muscle replicas is not possible yet. Our review shows that constant research in this field will resolve the existing constraints and enable successful cultured meat production in the near future. Therefore, production of cultured meat is a better solution that looks after environmental issues, spread of outbreaks, antibiotic resistance through the zoonotic spread, food and economic crises.

A New Symmetric Multilevel Inverter Topology Using Single and Double Source Sub-Multilevel Inverters

Kannan Ramani,Mohd. Ali Jagabar Sathik,Selvam Sivakumar 전력전자학회 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.1

In recent years, the multilevel converters have been given more attention due to their modularity, reliability, failure management and multi stepped output waveform with less total harmonic distortion. This paper presents a novel symmetric multilevel inverter topology with reduced switching components to generate a high quality stepped sinusoidal voltage waveform. The series and parallel combinations of switches in the proposed topology reduce the total number of conducting switches in each level of output voltages. In addition, a comparison between the proposed topology with another topology from the literature is presented. To verify the proposed topology, the computer based simulation model is developed using MATLAB/Simulink and experimentally with a prototype model results are then compared.

INTERCOMM 93 - 범 세계 지능망을 위한 인터 네트워킹

Pandurangan G.(Ramani) 한국정보통신기술협회 1993 국제전기통신표준화소식 Vol.27 No.-

기업과 무역의 국제화는 시대에 부응하는 요구이며, 원활하고 특징있는 국제 전기통신 서비스의 욕구를 창출하였다. 다양한 국가와 지역의 상이한 환경과 요구조건은 각 국가에서 변경없이 그대로 적용할 수 있는 단일 표준개발을 허용하지 않는다. 조화를 이룬 표준과 완전한 기능의 국제적 관문(gate ways)이 종단대 종단(end-to-end)망 및 서비스 연결성을 제공하는데 결정적인 요소가 되며, 특히 국경을 초월하여 발전하는 세계적인 지능 통신망 서비스와 관련되었을 때 그러하다. 이러한 세계적인 인터네트워킹 서비스에 대한 잠재성은 해결을 요하는 표준 및 정책문제를 제기한다. 현재 많은 표준화 기구가 이러한 작업의 조화를 이루고 시장의 수요에 반응하려는 노력을 진행하고 있음을 알 수 있다.

History of WFNS Spine Committee

Premanand Shantaram Ramani,Sumeet Sasane,Achal Gupta,Mehmet Zileli 대한척추신경외과학회 2021 Neurospine Vol.18 No.1

.