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Rak-Hun Jeong,이대영,Jin-Gyeong Cho,이상민,Hee-Cheol Kang,Woo-Duck Seo,Hwang-Won Kang,김지영,Nam-In Baek 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.6
Fresh and chopped aerial parts of Oryza sativa were extracted in 80% aqueous mehthanol, and the concentrated extract was successively partitioned using n-hexane, ethyl acetate (EtOAc), nbutanol, and H2O. From the EtOAc fraction, a new flavonolignan (2) and a known flavonolignan, salcolin B (1), were isolated through repeated SiO2 and octadecyl silica gel column chromatography. Based on NMR, mass spectrometer, and IR spectroscopic data, the chemical structure of compound (2) was determined to be tricin-4'-O-[erythro-β-guaiacyl-(7''-O-methyl)-glyceryl] ether, which has thus far never been reported and was named salcolin C (2). Salcolin B (1) and salcolin C (2) dose-dependently inhibited nitric oxide production in RAW 264.7 cells with IC50 values of 29.8±0.6 and 10.8±1.0 μM, respectively, without visible toxic effect.
( Rak Hun Jeong ),( Dae Young Lee ),( Jin Gyeong Cho ),( Sang Min Lee ),( Hee Cheol Kang ),( Woo Duck Seo ),( Hwang Won Kang ),( Ji Young Kim ),( Nam In Baek ) 한국응용생명화학회(구 한국농화학회) 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.6
Fresh and chopped aerial parts of Oryza sativa were extracted in 80% aqueous mehthanol, and the concentrated extract was successively partitioned using n-hexane, ethyl acetate (EtOAc), nbutanol, and H2O. From the EtOAc fraction, a new flavonolignan (2) and a known flavonolignan, salcolin B (1), were isolated through repeated SiO2 and octadecyl silica gel column chromatography. Based on NMR, mass spectrometer, and IR spectroscopic data, the chemical structure of compound (2) was determined to be tricin-4`-O-[erythro-β-guaiacyl-(7``-O-methyl)- glyceryl] ether, which has thus far never been reported and was named salcolin C (2). Salcolin B (1) and salcolin C (2) dose-dependently inhibited nitric oxide production in RAW 264.7 cells with IC50 values of 29.8±0.6 and 10.8±1.0 μM, respectively, without visible toxic effect.
( Hun Min Song ),( Gwang Hun Park ),( Hyun Ji Eo ),( Jin Wook Lee ),( Mi Kyoung Kim ),( Jeong Rak Lee ),( Man Hyo Lee ),( Jin Suk Koo ),( Jin Boo Jeong ) 한국응용약물학회 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.4
Naringenin (NAR) as one of the fl avonoids observed in grapefruit has been reported to exhibit an anti-cancer activity. However, more detailed mechanism by which NAR exerts anti-cancer properties still remains unanswered. Thus, in this study, we have shown that NAR down-regulates the level of cyclin D1 in human colorectal cancer cell lines, HCT116 and SW480. NAR inhibited the cell proliferation in HCT116 and SW480 cells and decreased the level of cyclin D1 protein. Inhibition of proteasomal degradation by MG132 blocked NAR-mediated cyclin D1 downregulation and the half-life of cyclin D1 was decreased in the cells treated with NAR. In addition, NAR increased the phosphorylation of cyclin D1 at threonine-286 and a point mutation of threonine-286 to alanine blocked cyclin D1 downregulation by NAR. p38 inactivation attenuated cyclin D1 downregulation by NAR. From these results, we suggest that NAR-mediated cyclin D1 downregulation may result from proteasomal degradation through p38 activation. The current study provides new mechanistic link between NAR, cyclin D1 downregulation and cell growth in human colorectal cancer cells.
New flavonolignan glucoside from the aerial parts of Oryza sativa
Jeong, Rak-Hun,Lee, Dae-Young,Cho, Jin-Gyeong,Seo, Kyeong-Hwa,Lee, Jae-Woong,Lee, Min-Ho,Seo, Woo-Duck,Kang, Hee-Cheol,Kim, Geum-Soog,Noh, Hyung-Jun,Lee, Youn-Hyung,Baek, and Nam-In Springer-Verlag 2014 Chemistry of natural compounds Vol.49 No.6
Jeong, Rak-Hun,Lee, Dae-Young,Cho, Jin-Gyeong,Lee, Sang-Min,Kang, Hee-Cheol,Seo, Woo-Duck,Kang, Hwang-Won,Kim, Ji-Young,Baek, Nam-In The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.6
Fresh and chopped aerial parts of $Oryza$ $sativa$ were extracted in 80% aqueous mehthanol, and the concentrated extract was successively partitioned using $n$-hexane, ethyl acetate (EtOAc), $n$-butanol, and $H_2O$. From the EtOAc fraction, a new flavonolignan (2) and a known flavonolignan, salcolin B (1), were isolated through repeated $SiO_2$ and octadecyl silica gel column chromatography. Based on NMR, mass spectrometer, and IR spectroscopic data, the chemical structure of compound (2) was determined to be tricin-4'-$O$-[erythro-${\beta}$-guaiacyl-(7"-$O$-methyl)-glyceryl] ether, which has thus far never been reported and was named salcolin C (2). Salcolin B (1) and salcolin C (2) dose-dependently inhibited nitric oxide production in RAW 264.7 cells with $IC_{50}$ values of $29.8{\pm}0.6$ and $10.8{\pm}1.0{\mu}m$, respectively, without visible toxic effect.
Song, Hun Min,Park, Gwang Hun,Eo, Hyun Ji,Lee, Jin Wook,Kim, Mi Kyoung,Lee, Jeong Rak,Lee, Man Hyo,Koo, Jin Suk,Jeong, Jin Boo The Korean Society of Applied Pharmacology 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.4
Naringenin (NAR) as one of the flavonoids observed in grapefruit has been reported to exhibit an anti-cancer activity. However, more detailed mechanism by which NAR exerts anti-cancer properties still remains unanswered. Thus, in this study, we have shown that NAR down-regulates the level of cyclin D1 in human colorectal cancer cell lines, HCT116 and SW480. NAR inhibited the cell proliferation in HCT116 and SW480 cells and decreased the level of cyclin D1 protein. Inhibition of proteasomal degradation by MG132 blocked NAR-mediated cyclin D1 downregulation and the half-life of cyclin D1 was decreased in the cells treated with NAR. In addition, NAR increased the phosphorylation of cyclin D1 at threonine-286 and a point mutation of threonine-286 to alanine blocked cyclin D1 downregulation by NAR. p38 inactivation attenuated cyclin D1 downregulation by NAR. From these results, we suggest that NAR-mediated cyclin D1 downregulation may result from proteasomal degradation through p38 activation. The current study provides new mechanistic link between NAR, cyclin D1 downregulation and cell growth in human colorectal cancer cells.
벼(Oryza sativa L.)의 지상부로부터 adenosine과 phlomuroside의 분리 및 동정
정락훈 ( Rak Hun Jeong ),이대영 ( Dae Young Lee ),조진경 ( Jin Gyeong Cho ),백윤수 ( Yoon Su Baek ),서경화 ( Kyeong Hwa Seo ),이동걸 ( Dong Geol Lee ),강희철 ( Hee Cheol Kang ),김지영 ( Ji Young Kim ),백남인 ( Nam In Baek ) 한국응용생명화학회(구 한국농화학회) 2014 Journal of Applied Biological Chemistry (J. Appl. Vol.57 No.4
벼 지상부를 MeOH 수용액으로 추출하고 이 추출물을 n-hexane, EtOAc, n-BuOH, 및 H2O 분획으로 나누었다. n-BuOH 분획에 대하여 silica gel, ODS 및 Sephadex LH-20 column chromatography 를 반복실시 하여 2종의 화합물을 분리하였다. NMR, IR 및 MS data를 해석하여 각각 adenosine (1)과 phlomuroside (2)로 구조동정 하였다. 이들 화합물들은 벼에서는 처음으로 분리되었다. Fresh and chopped aerial parts of Oryza sativa were extracted in 80% aqueous mehthanol, and the concentrated extract was successively partitioned in n-hexane, ethyl acetate (EtOAc), n-butanol (n-BuOH), and H2O fractions. From the n-BuOH fraction, two compounds were isolated through repeated silica gel and ODS column chromatography (c.c.). Based on nuclear magnetic resonance (NMR), mass spectrometry and infrared spectroscopy spectroscopic data, the compounds were identified to be adenosine (1) and phlomuroside (2). Especially, the configuration of both the anomer hydroxyl groups was determined as β from the coupling constants of the anomer protons (J =6.0 and 7.6 Hz) in the 1H-NMR spectra. This is the first report for the isolation of these compounds from Oryza sativa L.
순무(Brassica rapa ssp.) 뿌리로부터 flavonoid의 분리 및 동정
정락훈 ( Rak Hun Jeong ),( Qian Wu ),조진경 ( Jin Gyeong Cho ),이대영 ( Dae Young Lee ),( Sabina Shrestha ),이민호 ( Min Ho Lee ),이경태 ( Kyung Tae Lee ),최명숙 ( Myung Sook Choi ),정태숙 ( Tae Sook Jeong ),안은미 ( Eun Mi 한국응용생명화학회(구 한국농화학회) 2013 Journal of Applied Biological Chemistry (J. Appl. Vol.56 No.1
순무뿌리(Brassica rapassp)를 실온에서 95% ethanol 수용액으로 추출하고 이 추출물을 ethyl acetate (EtOAc)분획, n-butyl alcohol 분획 및 H2O 분획으로 나누었다. EtOAc분획에 대하여 silica gel, ODS 및 Sephadex LH-20 column chromatography 를 반복실시 하여 5종의 flavonoid를 분리하였다. NMR, IR 및 MS data를 해석하여 각각 lic℃halcone A (1), 4,4`-dihydroxy- 3`-methoxychalcone (2), liquiritigenin (3), liquiritin (4), isoliquiritin (5)으로 구조동정하였다. 이들 화합물들은 순무뿌리에서는 처음으로 분리되었다. The roots of Brassica rapa ssp. were extracted with 95% aqueous ethanol and the concentrated extracts were partitioned using ethyl acetate (EtOAc), n-butyl alcohol and H2O, successively. From the EtOAc fraction, five flavonoids were isolated through repeated silica gel and ℃tadecyl silica gel (ODS) column chromatography (c.c.). Based on NMR, mass spectrometry (MS) and IR spectroscopic data, the chemical structures of the compounds were determined to be lic℃halcone A (1), 4,4`- dihydroxy-3`-methoxychalcone (2), liquirtigenin (3), liquiritin (4), and isoliquiritin (5). This is the first report of these compounds isolated from the root of this plant.
벼(Oryza sativa L.) 지상부로부터 신규 Flavonolignans 및 Lignan glycosides의 분리 및 동정
정락훈(Rak-Hun Jeong),이대영(Dae-Young Lee),조진경(Jin-GyeongCho),Sabina Shrestha,박지해(Ji-Hae Park),서경화(Kyeong-Hwa Seo),이민호(Min-Ho Lee),노영덕(Yeong-Deok Rho),백남인(Nam-In Baek),이대영(Dae-Yung Lee),서우덕(Woo-Duck Seo),강 한국약용작물학회 2012 한국약용작물학회 학술대회논문집 Vol.2012 No.2