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Ganesan Raja,김시원,윤다혜,윤창신,김석만 대한화학회 2017 Bulletin of the Korean Chemical Society Vol.38 No.2
Mesoporous carbon nanoparticles (MCNs) have been applied in a variety of drug/gene carriers. In addition to their potential benefits, many studies of their potential toxicity have been reported, showing the limitations of metabolic contextualization. In this study, we conducted1H-nuclear magnetic resonance (NMR) profiling combined with statistical methods such as orthogonal partial least squares discriminant analysis and Pearson correlation analysis to assess metabolic alterations in the whole body of zebrafish (Danio rerio) in the presence of various concentrations of MCNs. The MCN exposure influenced numerous metabolites in energy metabolism (e.g., metabolites involved in glycolysis and tricarboxylic acid cycle) and disturbed the balance of neurotransmitters and osmoregulators. Our findings demonstrate the potential applicability of using a metabolomics approach to determine underlying metabolic disturbances caused by MCNs.
Ganesan Raja,김시원,윤다혜,윤창신,김석만 대한화학회 2018 Bulletin of the Korean Chemical Society Vol.39 No.1
Titanium dioxide nanoparticles (TiO2-NPs) are widely used in foods, cosmetics, and pharmaceuticals. In the present study, male and female zebrafish (Danio rerio) were exposed to sub-lethal concentrations of TiO2-NPs dosed at 0.1 mg/L (low) and 5.0 mg/L (high), respectively. 1H–nuclear magnetic resonance (NMR) profiling combined with univariate and multivariate statistics was used to assess metabolic alterations in the TiO2-NP-treated fish. Purine metabolism—including the anabolism/catabolism of ATP, ADP, AMP, xanthine, and inosine—was significantly influenced, which may indicate some genotoxicity resulting from TiO2-NP exposure. Increasing the dose and duration of TiO2-NP exposure led to additional metabolic disturbances in glycerophospholipid metabolism and the Krebs cycle. Our findings demonstrate the potential applicability of using a metabolomics approach to determine underlying metabolic disturbances caused by TiO2-NPs.
Valiyaparambath Musfir Mehaboob,Kunnampalli Faizal,Palusamy Raja,Ganesan Thiagu,Abubakker Aslam,Appakan Shajahan 한국식물생명공학회 2019 JOURNAL OF PLANT BIOTECHNOLOGY Vol.46 No.1
Ginger is an important monocotyledonous plant belonging to the family Zingiberaceae. The objective of this study was to investigate the regeneration potential of ginger using leaf base explants. Auxins such as 2, 4-D and NAA in combination with BA were used for initiation of callus. Different combinations of both ammonium (NH4+) and nitrate (NO3-) were also studied for efficient callus production. High frequency of white friable calli was observed on modified Murashige and Skoog (MS) medium supplemented with 2.0 mg/L 2, 4-D, 0.5 mg/L NAA and 0.5 mg/L BA. The highest shoot induction (92.33%), shootlets number (7.33± 0.33) and length (88.33±4.40) mm were achieved on MS media containing 0.5 mg/L BA. Regenerated shoots were transferred to in vitro rooting media containing 1.0 mg/L IBA. Afterwards, plantlets with well-developed root and shoot system were subjected to a twostep hardening process. 71% of plantlets survived after secondary hardening without any abnormal morphology.
Mehaboob, Valiyaparambath Musfir,Faizal, Kunnampalli,Raja, Palusamy,Thiagu, Ganesan,Aslam, Abubakker,Shajahan, Appakan The Korean Society of Plant Biotechnology 2019 식물생명공학회지 Vol.46 No.1
Ginger is an important monocotyledonous plant belonging to the family Zingiberaceae. The objective of this study was to investigate the regeneration potential of ginger using leaf base explants. Auxins such as 2, 4-D and NAA in combination with BA were used for initiation of callus. Different combinations of both ammonium ($NH^{4+}$) and nitrate ($NO^{3-}$) were also studied for efficient callus production. High frequency of white friable calli was observed on modified Murashige and Skoog (MS) medium supplemented with 2.0 mg/L 2, 4-D, 0.5 mg/L NAA and 0.5 mg/L BA. The highest shoot induction (92.33%), shootlets number ($7.33{\pm}0.33$) and length ($88.33{\pm}4.40$) mm were achieved on MS media containing 0.5 mg/L BA. Regenerated shoots were transferred to in vitro rooting media containing 1.0 mg/L IBA. Afterwards, plantlets with well-developed root and shoot system were subjected to a twostep hardening process. 71% of plantlets survived after secondary hardening without any abnormal morphology.
Won Sung Min,Lee Na Young,Oh Ki-Kwang,Gupta Haripriya,Sharma Satya Priya,Kim Kyung Hwan,Kim Byoung Kook,Joung Hyun Chae,Jeong Jin Ju,Ganesan Raja,Han Sang Hak,Yoon Sang Jun,Kim Dong Joon,Suk Ki Tae 한국미생물학회 2023 The journal of microbiology Vol.61 No.2
The progression and exacerbation of liver fibrosis are closely related to the gut microbiome. It is hypothesized that some probiotics may slow the progression of liver fibrosis. In human stool analysis [healthy group (n = 44) and cirrhosis group (n = 18)], difference in Lactobacillus genus between healthy group and cirrhosis group was observed. Based on human data, preventive and therapeutic effect of probiotics Lactobacillus lactis and L. rhamnosus was evaluated by using four mice fibrosis models. L. lactis and L. rhamnosus were supplied to 3,5-diethoxycarbonyl-1,4-dihydrocollidine or carbon tetrachloride-induced liver fibrosis C57BL/6 mouse model. Serum biochemical measurements, tissue staining, and mRNA expression in the liver were evaluated. The microbiome was analyzed in mouse cecal contents. In the mouse model, the effects of Lactobacillus in preventing and treating liver fibrosis were different for each microbe species. In case of L. lactis, all models showed preventive and therapeutic effects against liver fibrosis. In microbiome analysis in mouse models administered Lactobacillus, migration and changes in the ratio and composition of the gut microbial community were confirmed. L. lactis and L. rhamnosus showed preventive and therapeutic effects on the progression of liver fibrosis, suggesting that Lactobacillus intake may be a useful strategy for prevention and treatment.