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RISS 인기검색어
- 검색키워드
- 저자 : Qingyan Fang (검색결과 3 건)
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Qingyan Wu,Hao Yu,Xibi Fang,Yunyun Cheng,Lijie Dong,Wenzhen Wei,Gang Wang,Haoyu Fu,Songcai Liu,Linlin Hao 한국통합생물학회 2016 Animal cells and systems Vol.20 No.6
Bama Xiang pig (BM) and Tibetan mini-pig (TM) are used as experimental animals in China; however, the dwarf molecular mechanisms of these Chinese local pig breeds are unknown. IGFBP-3 affects animal growth, carcass and meat quality. The aim of this study was to identify the polymorphisms in the promoter of the IGFBP-3 and analyse their effect on the IGFBP-3 mRNA expression level in liver and muscle tissues. High-density single-nucleotide polymorphisms (SNPs) (31) and InDels (5) were detected in the promoter of the IGFBP-3 in the BM, TM and Junmu No. 1 White (JM, control) pig breeds from 114 individuals by re-sequencing. A perfect Linkage disequilibrium consisted of 13 SNPs was observed in the promoter region and 2 main haplotypes were identified, of which the h1 genotype (GCA-ATGTACATAT) was more prevalent in JM breed than in TM or BM breeds (P < .0001), h2 (ATGTGCACG--CGC) was the dominant haplotype in TM and BM breeds (P < .0001). Expression analysis showed that haplotype of the promoter region is highly associated with the IGFBP-3 mRNA expression level in liver and muscle tissues of pigs. The IGFBP-3 mRNA expression level was determined higher in the liver and muscle tissues of pigs with h2 genotype as compared to that in pigs with h1 genotype (P < .05). The results suggest that the SNPs and haplotypes in the promoter of the IGFBP-3 gene may serve as useful molecular markers for the body growth traits and the breeding in swine.
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SNPs in the 5’ terminal-region of IGFBP6 gene and its linkage with pig body size
Xibi Fang,Songcai Liu,Yunyun Cheng,Siming Li,Qingyan Wu,Dan Su,Chao Lu,Hao Yu,Linlin Hao 한국통합생물학회 2015 Animal cells and systems Vol.19 No.6
Insulin-like growth factor binding protein 6 (IGFBP6) is a key factor in regulating the effects of insulin-like growth factor 2 (IGF2) on the animal growth and development, but the mechanism is far from known. In this study, the 5’-terminal sequence of the IGFBP6 gene (from −920 to −1 bp) which may regulate gene expression was sequenced in the Bama mini-pigs, the Tibetan mini-pigs, the Landrace pigs, the Large White pigs and the Northeast wild boars to screen for the single nucleotide polymorphisms (SNPs) and analyze the relations with the body size traits of swine through a chi-square test analysis. The genotype frequencies of the SNPs in the 5’-terminal sequence have shown that c.-726C > T, c.-722T > C, c.-535C > A, c.- 488T > C, c.-403A > G, and c.-378T > C may be related to the dwarf traits of the Bama mini-pig and the Tibetan minipigs (P < .05). A haplotype analysis of the 5’-terminal sequence of the IGFBP6 gene in the Landrace pigs, the Large White pigs and the Northeast wild boars found the two SNPs at −403 nt and −378 nt were in linkage and formed three kinds of haplotypes; AT was the dominant haplotype and the haplotype block was not formed in the Bama mini-pigs and Tibetan mini-pigs. Above all, the SNPs and haplotypes of the 5’-terminal sequence of the IGFBP6 gene may be involved in the regulation of swine body size.
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Shaodan Jia,Lun Ma,Xinke Chen,Qingyan Fang,Cheng Zhang 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.116 No.-
Rotary spray drying technology with bypass flue is widely adopted to realize the zero-discharge of desulfurizationwastewater in coal-fired power stations. Significantly, the deflector structure of the dryingtower is an essential factor affecting the evaporation performance of desulfurization wastewater. Thispaper is aimed to study the effect of the angle and number of the deflectors on the evaporation performancein a drying tower of a 600 MW coal-fired station. Results show that the gas distributor dividesthe flue gas into internal and external parts, and the flue gas in the internal channel is the main factoraffecting the evaporation performance. The deflector angles (especially for the internal deflector) havea remarkable impact on the evaporation behaviors, while the deflector numbers just have a minor effecton that. When the deflector number is 24 and the angles of internal and external deflectors are set at 0and 45, respectively, a better evaporation performance is obtained with the weighted average time ofapproximately 0.17 s and the complete evaporation distance of approximately 3.93 m. These optimal settingsare adopted in the actual operation, and the industrial measured results show good performance isobtained.
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