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Peng Zhang,Qinghong Xue,Jing Ma,Jingjing Ren,Shuili Xia,Lu Zhang,Wen-Bin Wang,Suresh K. Tikoo,Enqi Du 대한수의학회 2017 Journal of Veterinary Science Vol.18 No.2
Bovine adenovirus type 3 (BAdV3) is being used in the development of potential vehicles for gene therapy and vectored vaccine. To that end, a more comprehensive description of BAdV3 biology is essential. In this study, we focused on the role of pIX in BAdV3 virion rescue after full-length BAdV3 genome transfection. Initially, pIX deletion or initiation codon mutation abolished the production of progeny virions, which suggested that pIX was essential for the rescue of BAdV3 containing a full-length genome. Moreover, through transfection of a panel of pIX mutant BAdV3 genomes, we observed that the conserved N-terminus and the putative leucine zipper element (PLZP) were essential for virion rescue, whereas the C-terminus following the coiled-coil domain was non-essential. In addition, swap of the PLZP element and its following region of BAdV3 pIX to corresponding domains of human adenovirus type 5 (HAdV5) did not affect virion production, whereas swap of the entire pIX abolished production of progeny virions. We suggest that failure of the full-length BAdV3 pIX swap might be due to species specificity of its N-terminus region before the PLZP element.
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Guoai He,Cunxiao He,Yu Liu,Chao Liu,Zhiqiang Fu,Shuanghui Xu,Xiaofei Sheng,Zhu Xiao,Qinghong Wen 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.9
Dynamic softening behavior has been of great concern in the aluminum alloys, which served a vital role in affecting themicrostructure, deformation resistance and the final mechanical properties. In this paper, spray deposition was applied tomanufacture AlZnMgCu alloy to refine the casting microstructure and reduce segregation. The microstructure responses andcorresponding mechanism during the thermal compression with various temperatures and various strain rates were multiscalecharacterized and uncovered using high-resolution EBSD. The results revealed that many broken and fine grains wereobserved in the uneven and jagged grain boundaries under the low temperatures and high strain rates, which indicated theoccurrence of partial discontinuous dynamic recrystallization (DDRX). In addition, high density dislocations were formedat original grain boundaries, where great amount of low angle grain boundaries (LAGBs) were appeared. With temperatureincreased and strains rate decreased, the LAGBs would evolve to the high angle grain boundaries (HAGBs) owing to theconsiderable dislocations migrated to the grain boundaries then accumulated, which triggered the occurrence of continuousdynamic recrystallization (CDRX). Two different dynamic recrystallization types were observed and factors that influenceddifferent dynamic recrystallization behaviors were clearly elaborated, and the corresponding mechanism was indicateddetailly.
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