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      • Identifying of Digital Signals Based on Manifold Learning

        Qingbo Ji,Boyang Feng,Yun Lin,Zheng Dou,Zhiqiang Wu,Zhiping Zhang 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.2

        Modulation type is one of the most important characteristics used in signal recognition. An algorithm to realize signal modulation identification is proposed in this paper. We applied wavelet transformation and STFT to the signal, and then used manifold learning method to reduce the high dimension and extracted the recognition feature. The proper threshold value was set as the classifier to achieve the purpose of recognizing 4 kinds of signals (MASK, MFSK, MPSK,QAM) in Gauss white noise environment. The algorithm requires priori signal information no other than signal-to-noise rate. Simulation result indicates the algorithm achieves good performance.

      • Stress-induced expression of the sweetpotato gene <i>IbLEA14</i> in poplar confers enhanced tolerance to multiple abiotic stresses

        Ke, Qingbo,Park, Sung-Chul,Ji, Chang Yoon,Kim, Ho Soo,Wang, Zhi,Wang, Shiwen,Li, Hongbing,Xu, Bingcheng,Deng, Xiping,Kwak, Sang-Soo Elsevier 2018 Environmental and experimental botany Vol.156 No.-

        <P><B>Abstract</B></P> <P>Late embryogenesis abundant (LEA) proteins are small, highly hydrophilic proteins that act as protectors of macromolecules and increase abiotic stress tolerance in plants. We previously reported that overexpressing sweetpotato <I>IbLEA14</I> under the control of the <I>CaMV 35S</I> promoter increased osmotic and salt stress tolerance in transgenic sweetpotato calli. In this study, we generated transgenic poplar plants (<I>Populus alba × P. glandulosa</I>) expressing <I>IbLEA14</I> under the control of the oxidative stress-inducible <I>SWPA2</I> promoter (referred to as SL plants). Among the 15 SL plants obtained, three lines (SL2, SL7, and SL12) were established based on <I>IbLEA14</I> transcript levels, tolerance to salt stress and Southern blot analysis. The SL plants exhibited less damage in response to methyl viologen-mediated oxidative stress than non-transgenic (NT) plants. SL plants also showed enhanced tolerance to drought, salt, and heat stress, which was associated with higher photosystem II efficiency and lower malondialdehyde levels compared with NT plants. Furthermore, SL plants had higher levels of monolignol biosynthesis-related gene transcripts under drought stress compared with NT plants. Finally, SL plants exhibited increased tolerance to heat stress, which is associated with the high thermostability of IbLEA14 protein. SL plants might be useful for reforestation on global marginal lands, including desertification and reclaimed areas.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>IbLEA14</I> gene was transformed into poplar plants. </LI> <LI> Transgenic poplars exhibit increased tolerance to MV-mediated oxidative, drought, salt and heat stress. </LI> <LI> Overproduction of IbLEA14 affects lignification and thermostability of transgenic poplars. </LI> </UL> </P>

      • Down‐regulation of <i>GIGANTEA</i> ‐ <i>like</i> genes increases plant growth and salt stress tolerance in poplar

        Ke, Qingbo,Kim, Ho Soo,Wang, Zhi,Ji, Chang Yoon,Jeong, Jae Cheol,Lee, Haeng‐,Soon,Choi, Young‐,Im,Xu, Bingcheng,Deng, Xiping,Yun, Dae‐,Jin,Kwak, Sang‐,Soo John Wiley and Sons Inc. 2017 Plant biotechnology journal Vol.15 No.3

        <P><B>Summary</B></P><P>The flowering time regulator GIGANTEA (GI) connects networks involved in developmental stage transitions and environmental stress responses in <I>Arabidopsis</I>. However, little is known about the role of GI in growth, development and responses to environmental challenges in the perennial plant poplar. Here, we identified and functionally characterized three <I>GI‐like</I> genes (<I>PagGIa</I>,<I> PagGIb</I> and <I>PagGIc)</I> from poplar (<I>Populus alba × Populus glandulosa</I>). <I>PagGIs</I> are predominantly nuclear localized and their transcripts are rhythmically expressed, with a peak around zeitgeber time 12 under long‐day conditions. Overexpressing <I>PagGIs</I> in wild‐type (WT) <I>Arabidopsis</I> induced early flowering and salt sensitivity, while overexpressing <I>PagGIs</I> in the <I>gi‐2</I> mutant completely or partially rescued its delayed flowering and enhanced salt tolerance phenotypes. Furthermore, the PagGIs‐PagSOS2 complexes inhibited PagSOS2‐regulated phosphorylation of PagSOS1 in the absence of stress, whereas these inhibitions were eliminated due to the degradation of PagGIs under salt stress. Down‐regulation of <I>PagGIs</I> by RNA interference led to vigorous growth, higher biomass and enhanced salt stress tolerance in transgenic poplar plants. Taken together, these results indicate that several functions of <I>Arabidopsis GI</I> are conserved in its poplar orthologues, and they lay the foundation for developing new approaches to producing salt‐tolerant trees for sustainable development on marginal lands worldwide.</P>

      • Suppression of the <i>β-carotene hydroxylase</i> gene increases β-carotene content and tolerance to abiotic stress in transgenic sweetpotato plants

        Kang, Le,Ji, Chang Yoon,Kim, Sun Ha,Ke, Qingbo,Park, Sung-Chul,Kim, Ho Soo,Lee, Hyeong-Un,Lee, Joon Seol,Park, Woo Sung,Ahn, Mi-Jeong,Lee, Haeng-Soon,Deng, Xiping,Kwak, Sang-Soo Elsevier 2017 Vol. No.

        <P><B>Abstract</B></P> <P>β-carotene, a carotenoid that plays a key photo-protective role in plants is converted into zeaxanthin by β-carotene hydroxylase (CHY-β). Previous work showed that down-regulation of <I>IbCHY-β</I> by RNA interference (RNAi) results in higher levels of β-carotene and total carotenoids, as well as salt stress tolerance, in cultured transgenic sweetpotato cells. In this study, we introduced the RNAi-<I>IbCHY-β</I> construct into a white-fleshed sweetpotato cultivar (cv. Yulmi) by <I>Agrobacterium</I>-mediated transformation. Among the 13 resultant transgenic sweetpotato plants (referred to as RC plants), three lines were selected for further characterization on the basis of <I>IbCHY-β</I> transcript levels. The RC plants had orange flesh, total carotenoid and β-carotene contents in storage roots were 2-fold and 16-fold higher, respectively, than those of non-transgenic (NT) plants. Unlike storage roots, total carotenoid and β-carotene levels in the leaves of RC plants were slightly increased compared to NT plants. The leaves of RC plants also exhibited tolerance to methyl viologen (MV)-mediated oxidative stress, which was associated with higher 2,2-diphenyl-1- picrylhydrazyl (DPPH) radical-scavenging activity. In addition, RC plants maintained higher levels of chlorophyll and higher photosystem II efficiency than NT plants after 250 mM NaCl stress. Yield of storage roots did not differ significantly between RC and NT plants. These observations suggest that RC plants might be useful as a nutritious and environmental stress-tolerant crop on marginal lands around the world.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Transgenic sweet potato plants were generated by RNAi silencing of the <I>IbCHY-β</I> gene. </LI> <LI> Transgenic sweet potato plants had increased β-carotene and total carotenoid content. </LI> <LI> Transgenic sweet potato plants exhibited strong antioxidant capacity and salt stress tolerance. </LI> </UL> </P>

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