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Glioma-Associated Oncogene Homolog1 (Gli1)-Aquaporin1 pathway promotes glioma cell metastasis
( Zheng Qiang Liao ),( Ming Ye ),( Pei Gen Yu ),( Chun Xiao ),( Feng Yun Lin ) 생화학분자생물학회(구 한국생화학분자생물학회) 2016 BMB Reports Vol.49 No.7
Glioma-Associated Oncogene Homolog1 (Gli1) is known to be activated in malignant glioma; however, its downstream pathway has not been fully explained. The aim of this study was to explore the role of Gli1-Aquaporin1 (AQP1) signal pathway in glioma cell survival. Our data suggests that both Gli1 and AQP1 are upregulated in glioma tissues, as in comparison to in normal tissues. These up-regulation phenomena were also observed in glioma U251 and U87 cells. It was demonstrated that Gli1 positively regulated the AQP1 expression. By luciferase reporter gene and ChIP assay, we observed that this modulation process was realized by combination of Gli1 with AQP1 promotor. In addition, knock down of Gli1 by siRNA interference reduced the viability of glioma cells as well as suppressed cell metastasis. Also, the inhibitory effects of cell survival by silenced Gli1 were abrogated by AQP1 overexpression. In summary, glioma cell survival is a regulatory process and can be mediated by Gli1-AQP1 pathway. [BMB Reports 2016; 49(7): 394-399]
Xiao-Li Liao,Jun-Bao Wei,Yong-Qiang Li,Jian-Hong Zhong,Cheng-Cheng Liao,Chang-Yuan Wei 대한영상의학회 2018 Korean Journal of Radiology Vol.19 No.6
Objective: To perform a meta-analysis to quantitatively assess functional magnetic resonance imaging (MRI) in the diagnosis of locally recurrent prostate cancer. Materials and Methods: A comprehensive search of the PubMed, Embase, Cochrane Central Register of Controlled Trials, and Cochrane Database of Systematic Reviews was conducted from January 1, 1995 to December 31, 2016. Diagnostic accuracy was quantitatively pooled for all studies by using hierarchical logistic regression modeling, including bivariate modeling and hierarchical summary receiver operating characteristic (HSROC) curves (AUCs). The Z test was used to determine whether adding functional MRI to T2-weighted imaging (T2WI) results in significantly increased diagnostic sensitivity and specificity. Results: Meta-analysis of 13 studies involving 826 patients who underwent radical prostatectomy showed a pooled sensitivity and specificity of 91%, and the AUC was 0.96. Meta-analysis of 7 studies involving 329 patients who underwent radiotherapy showed a pooled sensitivity of 80% and specificity of 81%, and the AUC was 0.88. Meta-analysis of 11 studies reporting 1669 sextant biopsies from patients who underwent radiotherapy showed a pooled sensitivity of 54% and specificity of 91%, and the AUC was 0.85. Sensitivity after radiotherapy was significantly higher when diffusion-weighted MRI data were combined with T2WI than when only T2WI results were used. This was true when meta-analysis was performed on a per-patient basis (p = 0.027) or per sextant biopsy (p = 0.046). A similar result was found when 1H-magnetic resonance spectroscopy (1H-MRS) data were combined with T2WI and sextant biopsy was the unit of analysis (p = 0.036). Conclusion: Functional MRI data may not strengthen the ability of T2WI to detect locally recurrent prostate cancer in patients who have undergone radical prostatectomy. By contrast, diffusion-weight MRI and 1H-MRS data may improve the sensitivity of T2WI for patients who have undergone radiotherapy.
Numerical study of wake and aerodynamic forces on a twin-box bridge deck with different gap ratios
Jingmiao Shang,Qiang Zhou,Haili Liao,Allan Larsen,Jin Wang,Mingshui Li 한국풍공학회 2020 Wind and Structures, An International Journal (WAS Vol.30 No.4
Two-dimensional Delayed Detached Eddy Simulation (DDES) was carried out to investigate the uniform flow over a twin-box bridge deck (TBBD) with various gap ratios of L/C=5.1%, 12.8%, 25.6%, 38.5%, 73.3% and 108.2% (L: the gap-width between two girders, C: the chord length of a single girder) at Reynolds number, Re=4×104. The aerodynamic coefficients of the prototype deck with gap ratio of 73.3% obtained from the present simulation were compared with the previous experimental and numerical data for different attack angles to validate the present numerical method. Particular attention is devoted to the fluctuating pressure distribution and forces, shear layer reattachment position, wake velocity and flow pattern in order to understand the effects of gap ratio on dynamic flow interaction with the twin-box bridge deck. The flow structure is sensitive to the gap, thus a change in L/C thus leads to single-side shedding regime at L/C≤25.6%, and co-shedding regime at L/C≥35.8% distinguished by drastic changes in flow structure and vortex shedding. The gap-ratio-dependent Strouhal number gradually increases from 0.12 to 0.27, though the domain frequencies of vortices shedding from two girders are identical. The mean and fluctuating pressure distributions is significantly influenced by the flow pattern, and thus the fluctuating lift force on two girders increases or decreases with increasing of L/C in the single-side shedding and co-shedding regime, respectively. In addition, the flow mechanisms for the variation in aerodynamic performance with respect to gap ratios are discussed in detail.
A facile pyrolysis method to prepare vanadium oxides for high performance aqueous Zn-ion battery
Zhu Haitao,Liao Shengyun,Su Boya,Ding Xiaohui,Liu Qiang 한국물리학회 2022 Current Applied Physics Vol.34 No.-
Vanadium oxides, as one of the most promising cathode materials for zinc ion batteries, have attracted extensive attention in recent years. Different from the generally used hydrothermal and solvothermal methods to adjust the composition, structure, morphology and electrical properties of vanadium oxides, we firstly adopt a simple pyrolysis method to synthesize a series of vanadium oxides and use them as cathode materials for aqueous Zn-ion battery, whose electrochemical performances is superior to most state-of-the-art vanadium oxides. The asobtained V4O7 under the calcination temperature of 700 ◦C exhibits excellent zinc ion storage performance with maximum specific capacity of 367.2 mAh g 1 at the current density of 1 A g 1, about 84.9% capacity retention after 100 cycles, excellent rate performance, high capacity. In addition, a series of structural and electrochemical characterization are used to reveal the possible mechanism of charge and discharge.
Dihydroartemisinin inhibits HepG2.2.15 proliferation by inducing cellular senescence and autophagy
( Jiang Zou ),( Qiang Ma ),( Ru Sun ),( Jiajing Cai ),( Hebin Liao ),( Lei Xu ),( Jingruo Xia ),( Guangcheng Huang ),( Lihua Yao ),( Yan Cai ),( Xiaowu Zhong ),( Xiaolan Guo ) 생화학분자생물학회(구 한국생화학분자생물학회) 2019 BMB Reports Vol.52 No.8
Dihydroartemisinin (DHA) has been reported to possess anti-cancer activity against many cancers. However, the pharmacologic effect of DHA on HBV-positive hepatocellular carcinoma (HCC) remains unknown. Thus, the objective of the present study was to determine whether DHA could inhibit the proliferation of HepG2.2.15 cells and uncover the underlying mechanisms involved in the effect of DHA on HepG2.2.15 cells. We found that DHA effectively inhibited HepG2.2.15 HCC cell proliferation both in vivo and in vitro. DHA also reduced the migration and tumorigenicity capacity of HepG2.2.15 cells. Regarding the underlying mechanisms, results showed that DHA induced cellular senescence by up-regulating expression levels of proteins such as p-ATM, p-ATR, γ-H<sub>2</sub>AX, P53, and P21 involved in DNA damage response. DHA also induced autophagy (green LC3 puncta gathered together and LC3II/LC3I ratio increased through AKT-mTOR pathway suppression). Results also revealed that DHA-induced autophagy was not linked to senescence or cell death. TPP1 (telomere shelterin) overexpression could not rescue DHA-induced anticancer activity (cell proliferation). Moreover, DHA down-regulated TPP1 expression. Gene knockdown of TPP1 caused similar phenotypes and mechanisms as DHA induced phenotypes and mechanisms in HepG2.2.15 cells. These results demonstrate that DHA might inhibit HepG2.2.15 cells proliferation through inducing cellular senescence and autophagy. [BMB Reports 2019; 52(8): 520-525]
Gong, Chen,Liao, Hui,Wang, Jiang,Lin, Yang,Qi, Jun,Qin, Liang,Tian, Lin-Qiang,Guo, Feng-Jing Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7
Osteosarcoma, the most common primary mesenchymal malignant tumor, usually has bad prognosis in man, with cancer stem-like cells (CSCs) considered to play a critical role in tumorigenesis and drug-resistance. It is known that phosphatidylinositol 3-kinase (PI3K) is involved in regulation of tumor cell fates, such as proliferation, cell cycling, survival and apoptosis. Whether and how PI3K and inhibitors might cooperate in human osteosarcoma CSCs is still unknown. We therefore evaluated the effects of LY294002, a PI3K inhibitor, on the cell cycle and apoptosis of osteosarcoma CSCs in vitro. LY294002 prevented phosphorylation of protein kinase B (PKB/Akt) by inhibition of PI3K phosphorylation activity, thereby inducing G0/G1 cell cycle arrest and apoptosis in osteosarcoma CSCs. Further studies also demonstrated that apoptosis induction by LY294002 is accompanied by activation of caspase-9, caspase-3 and PARP, which are involved in the mitochondrial apoptosis pathway. Therefore, our results indicate PI3K inhibitors may represent a potential strategy for managing human osteosarcoma via affecting CSCs.
Li, Zhiguo,Zhou, Qiang,Liao, Haili,Ma, Cunming Techno-Press 2018 Wind and Structures, An International Journal (WAS Vol.26 No.5
A numerical study based on a delayed detached eddy simulation (DDES) is conducted to investigate the aerodynamic mechanism behind the suppression of vortex-induced vibrations (VIVs) of twin box girders by central grids, which have an inhibition effect on VIVs, as evidenced by the results of section model wind tunnel tests. The mean aerodynamic force coefficients with different attack angles are compared with experimental results to validate the numerical method. Next, the flow structures around the deck and the aerodynamic forces on the deck are analyzed to enhance the understanding of the occurrence of VIVs and the suppression of VIVs by the application of central grids. The results show that shear layers are separated from the upper railings and lower overhaul track of the upstream girder and induce large-scale vortices in the gap that cause periodical lift forces of large amplitude acting on the downstream girder, resulting in VIVs of the bridge deck. However, the VIVs are apparently suppressed by the central grids because the vortices in the central gap are reduced into smaller vortices and become weaker, causing slightly fluctuating lift forces on the deck. In addition, the mean lift force on the deck is mainly caused by the upstream girder, whereas the fluctuating lift force is mainly caused by the downstream girder.