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프로토 타입 조직등가비례계수기의 중입자가속기연구소의 135 MeV/u 탄소 이온에 대한 선형에너지 스펙트럼 측정
남욱원 ( Uk Won Nam ),이재진 ( Jae Jin Lee ),표정현 ( Jeong Hyun Pyo ),박원기 ( Won Kee Park ),문봉곤 ( Bong Kon Moon ),임창휘 ( Chang Hwy Lim2,),문명국 ( Myung Kook Moon ),하시시키다무라 ( Hisashi Kitamure ),신고고바야시 ( Shingo Kob 한국센서학회 2014 센서학회지 Vol.23 No.3
TEPC (Tissue Equivalent Proportional Counter) was usually used for high LET radiation dosimetry. We developed a prototype TEPC for micro-dosimetry in the range of 0.2~300 keV/μm. And, the simulated site diameter of the TEPC is 2 μm, of similar size to a cell nucleus. For purposes of characterization the response for high LET radiation of the TEPC has been investigated under 135MeV/u Carbon ions in HIMAC (Heavy Ion Medical Accelerator). We determined the gas multiplication factor and measured the lineal energy spectrum [yd(y)] of 135 MeV/u Carbon ions. The value of the gas multiplication factor was 315 at 700 V bias voltage. As a result of the experiment, we could more understand the performance of the TEPC for high LET (Linear Energy Transfer) radiation. And the procedure of high LET radiation dosimetry using TEPC is established.
Lim, Yong,Lee, Mi-Yea,Jung, Jae-Kyung,Pyo, Myoung-Yun,Yun, Yeo-Pyo The Korean Society of Food Hygiene and Safety 2011 한국식품위생안전성학회지 Vol.26 No.3
Platelet derived growth factor (PDGF)-BB is one of the most potent vascular smooth muscle cell(VSMC) proliferative factors, and abnormal VSMC proliferation by PDGF-BB plays an important role in the development and progression of atherosclerosis. The aim of this study was to assess the effect of YP 12, a newly synthesized obovatol derivative, on the proliferation of PDGF-BB-stimulated rat aortic VSMCs. The anti-proliferative effects of YP 12 on rat aortic VSMCs were examined by direct cell counting and by using $[^3H]$ thymidine incorporation assays. It was found that YP 12 potently inhibited the growth of VSMCs. The pre-incubation of YP 12 (1-4 ${\mu}M$) significantly inhibited the proliferation and DNA synthesis of 25 ng/ml PDGF-BB-stimulated rat aortic VSMCs in a concentration-dependent manner. In accordance with these findings, YP 12 revealed blocking of the PDGF-BB-inducible progression through G0/G1 to S phase of the cell cycle in synchronized cells. Whereas, YP 12 did not show any cytotoxicity in rat aortic VSMCs in this experimental condition by WST-1 assay. These results also show that YP 12 may have potential as an anti-proliferative agent for the treatment of restenosis and atherosclerosis.
Lim, Geon-Pyo,Park, Chan-Wook,Labios, Remund,Yoon, Yong-Beom Korea Electric Power Corporation 2015 KEPCO Journal on electric power and energy Vol.1 No.1
Energy storage systems (ESS) can be used to provide frequency regulation services in a power system to replace traditional frequency regulation power plants. Battery ESS, in particular, can provide "fast-responding frequency regulation," wherein the facility can respond immediately and accurately to the frequency regulation signal sent by the system operator. This paper presents the development and the trial run results of a frequency regulation control system that uses large-scale ESS for use in a large power system. The control system was developed initially for the 4 MW ESS demonstration facility in Jocheon Jeju Island, and was further developed for use in the 28 MW ESS facility at the Seo-Anseong substation and the 24 MW ESS facility at the Shin-Yongin substation to provide frequency regulation services within mainland Korea. The ESS facility in Seo-Anseong substation responds to a sudden drop in frequency via governor-free control, while the ESS facility in Shin-Yongin responds via automatic generator control (AGC).
Toward unraveling the structure of <i>Brassica rapa</i> genome
Lim, Yong Pyo,Plaha, Prikshit,Choi, Su Ryun,Uhm, Taesik,Hong, Chang Pyo,Bang, Jae Wook,Hur, Yoon Kang Blackwell Publishing Ltd 2006 Physiologia plantarum Vol.126 No.4
<P>Genomic research in any organism encompasses understanding structure of the target genome and genes, their function, and evolution. <I>Brassica rapa</I>, which is phylogenetically related to <I>Arabidopsis thaliana</I>, is an important species with respect to its uses as vegetable, oil, and fodder. The availability of suitable genetic and genomic resources is a prerequisite to undertake genomic research in <I>B. rapa</I>. We have developed reference mapping populations of Chinese cabbage (<I>B. rapa</I> ssp. <I>pekinensis</I>) comprising 78 doubled haploid lines and over 250 recombinant inbred lines. Two Bacterial Artificial Chromosome (BAC) libraries, generated by restriction enzymes <I>Hin</I>dIII (KBrH) and <I>Bam</I>HI (KBrB), comprise 56 592 and 50 688 clones, respectively. We have also constructed 22 cDNA libraries from different plant tissues consisting of 104 914 clones with an average length of 575 bp. Initial BAC-end sequence analysis of 1473 clones of the KBrH library led us to understand the structure of <I>B. rapa</I> genome with respect to extent of genic sequences and their annotation, and relative abundance of different types of repetitive DNAs. Full-length sequence analysis of BAC clones revealed extensive triplication of <I>B. rapa</I> DNA segments coupled with variable gene losses within the segments. The formulation of the ‘Multinational Brassica Genome Project’ has laid the foundation to sequence the complete genome of <I>B. rapa</I> ssp. <I>pekinensis</I> by the international Brassica research community. It has been proposed to undertake BAC-to-BAC sequencing of genetically mapped seed BACs. In recent years, development of bioinformatics tools in <I>Brassica</I> has given a boost to structural genomics research in <I>Brassica</I> species. The research undertaken with the availability of various genomic resources in the public domain has added to our understanding of the structure of <I>B. rapa</I>.</P>
Effect of exposure to interleukin-21 at various time points on human natural killer cell culture
Lim, Dong-Pyo,Jang, Youn-Young,Kim, Seokho,Koh, Sang Seok,Lee, Je-Jung,Kim, Ju-Sun,Thi Phan, Minh-Trang,Shin, Dong-Jun,Shin, Myung-Geun,Lee, Seung-Hwan,Yoon, Meesun,Kim, Sang-Ki,Yoon, Jung-Han,Park, M Elsevier 2014 cytotherapy Vol.16 No.10
<P>Interleukin-21 (IL-21) can enhance the effector function of natural killer (NK) cells but also limits their proliferation when continuously combined with IL-2/IL-15. Paradoxically, membrane-bound (mb)-IL-21 has been shown to improve human NK cell proliferation when cultured with IL-2/mb-IL-15. To clarify the role of IL-21, we investigated the effect of the timing of IL-21 addition to NK cell culture.</P>
Characterization of rDNAs and tandem repeats in the heterochromatin of Brassica rapa.
Lim, Ki-Byung,de Jong, Hans,Yang, Tae-Jin,Park, Jee-Young,Kwon, Soo-Jin,Kim, Jung Sun,Lim, Myung-Ho,Kim, Jin A,Jin, Mina,Jin, Yong-Moon,Kim, Seog Hyung,Lim, Yong Pyo,Bang, Jae-Wook,Kim, Ho-Il,Park, Be Korean Society for Molecular Biology 2005 Molecules and cells Vol.19 No.3
<P>We describe the morphology and molecular organization of heterochromatin domains in the interphase nuclei, and mitotic and meiotic chromosomes, of Brassica rapa, using DAPI staining and fluorescence in situ hybridization (FISH) of rDNA and pericentromere tandem repeats. We have developed a simple method to distinguish the centromeric regions of mitotic metaphase chromosomes by prolonged irradiation with UV light at the DAPI excitation wavelength. Application of this bleached DAPI band (BDB) karyotyping method to the 45S and 5S rDNAs and 176 bp centromere satellite repeats distinguished the 10 B. rapa chromosomes. We further characterized the centromeric repeat sequences in BAC end sequences. These fell into two classes, CentBr1 and CentBr2, occupying the centromeres of eight and two chromosomes, respectively. The centromere satellites encompassed about 30% of the total chromosomes, particularly in the core centromere blocks of all the chromosomes. Interestingly, centromere length was inversely correlated with chromosome length. The morphology and molecular organization of heterochromatin domains in interphase nuclei, and in mitotic and meiotic chromosomes, were further characterized by DAPI staining and FISH of rDNA and CentBr. The DAPI fluorescence of interphase nuclei revealed ten to twenty conspicuous chromocenters, each composed of the heterochromatin of up to four chromosomes and/or nucleolar organizing regions.</P>
Lim, Geon-Pyo,Choi, Yo-Han,Park, Chan-Wook,Kim, Soo-Yeol,Chang, Byung-Hoon,Labios, Remund Korea Electric Power Corporation 2016 KEPCO Journal on electric power and energy Vol.2 No.2
To test the effectiveness of using an energy storage system for frequency regulation, the Energy New Business Laboratory at KEPCO Research Institute installed a 4 MW energy storage system (ESS) demonstration facility at the Jocheon Substation on Jeju Island. And after the successful completion of demonstration operations, a total of 52 MW ESS for frequency regulation was installed in Seo-Anseong (28 MW, governor-free control) and in Shin-Yongin (24 MW, automatic generation control). The control system used in these two sites was based on the control system developed for the 4 MW ESS demonstration facility. KEPCO recently finished the construction of 184 MW ESS for frequency regulation in 8 locations, (e.g. Shin-Gimjae substation, Shin-Gaeryong substation, etc.) and they are currently being tested for automatic operation. KEPCO plans to construct additional ESS facilities (up to a total of about 500 MW for frequency regulation by 2017), thus, various operational tests would first have to be conducted. The high-speed characteristic of ESS can negatively impact the power system in case the 500 MW ESS is not properly operated. At this stage we need to verify how effectively the 500 MW ESS can regulate frequency. In this paper, the effect of using ESS for frequency regulation on the power system of Korea was studied. Simulations were conducted to determine the effect of using a 524 MW ESS for frequency regulation. Models of the power grid and the ESS were developed to verify the performance of the operation system and its control system. When a high capacity power plant is tripped, a 24 MW ESS supplies power automatically and 4 units of 125MW ESS supply power manually. This study only focuses on transient state analysis. It was verified that 500 MW ESS can regulate system frequency faster and more effectively than conventional power plants. Also, it was verified that time-delayed high speed operations of multiple ESS facilities do not negatively impact power system operations. It is recommended that further testing be conducted for a fleet of multiple ESSs with different capacities distributed over multiple substations (e.g. 16, 24, 28, and 48 MW ESS distributed across 20 substations) because each ESS measures frequency individually. The operation of one ESS facility will differ from the other ESSs within the fleet, and may negatively impact the performance of the others. The following are also recommended: (a) studies wherein all ESSs should be operated in automatic mode; (b) studies on the improvement of individual ESS control; and (c) studies on the reapportionment of all ESS energies within the fleet.
Lim, Yong-Pyo,Park, Kwang-Tae The Korean Society of Ginseng 1990 Journal of Ginseng Research Vol.14 No.2
This study was focused on the characterization of mitochondrial DNA (mtDNA) for molecular 9enetical approach of energy Production related mechanism in Panax ginseng. The simple and efficient method of mtDNA isolation from ginseng has been developed by modification of recently advanced methods. This procedure can successfully apply to mtDNA isolation of several plants. mtDNA of etiolated shoot and one-year root were digested with restriction endonucleases, but that of 6-year root not. Any difference was not observed in the restriction endonuclease digestion patterns among the ginseng variants. Molecular size of ginseng mtDNA was estimated at least 159 kb by the restriction endonuclease fragment analysis. The 4.5 kb extra band at the lane of EcoRII treatment could be observed in restriction patterns digested with the methylation sensitive endonucleases, BstN I and EcoRII. For construction of mitochondrial genomic library of ginseng, mtDNA was partially digested with EcoRl, and packaged with EMBL4 phage vector. Genomic library was screened and purified for further research including restriction mapping of ginseng mtDNA, and cloning of the genes. The gene of ATP synthase A subunit was cloned from the purified EMBL4 library clone No. 16. Now, clone No. 16 is subcloned for structure gene sequence analysis.