Valorization of polyhydroxyalkanoates production process by co-synthesis of value-added products

Kumar, Prasun,Kim, Beom Soo Elsevier 2018 Bioresource technology Vol.269 No.-

<P><B>Abstract</B></P> <P>Polyhydroxyalkanoates (PHAs) are the only polyesters that are completely synthesized biologically and possess features equivalent to petroleum-based plastics besides being biodegradable. PHA based materials may certainly prove helpful in addressing the concerns caused due to the indiscriminate use of synthetic plastics. However, the cost of producing these polymers on a large scale is still uneconomical. Various approaches have been developed to tackle this issue through usage of agro-industrial wastes, co-production of high market value products, polymer extraction using green solvents, etc. The advent of recombineering and CRISPR technologies has broadened the scope of constructing a microbe capable of synthesizing multiple products with economic feasibility. Quite a few high-market value chemicals are possible to synthesize along with the favorable accumulation of PHA. The present article attempts to review all PHA polymer co-production processes with other chemicals reported till date and discusses the opportunities for their large-scale operation in future.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Technical obstacles and solutions in economic production of PHA are discussed. </LI> <LI> Current scenario of various integrative approaches has been presented. </LI> <LI> Biorefinery has a central role in the overall improvement of circular bioeconomy. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Ecobiotechnological Approach for Exploiting the Abilities of Bacillus to Produce Co-polymer of Polyhydroxyalkanoate.

Kumar, Prasun,Singh, Mamtesh,Mehariya, Sanjeet,Patel, Sanjay K S,Lee, Jung-Kul,Kalia, Vipin C Association of Microbiologists of India 2014 Indian journal of microbiology Vol.54 No.2

<P>Ecobiotechnological approach is an attractive and economical strategy to enrich beneficial microbes on waste biomass for production of Polyhydroxyalkanoate (PHA). Here, six strains of Bacillus spp. were used to produce co-polymers of PHA from pea-shells. Of the 57 mixed bacterial cultures (BCs) screened, two of the BCs, designated as 5BC1 and 5BC2, each containing 5 strains could produce PHA co-polymer at the rate of 505-560?mg/l from feed consisting of pea-shell slurry (PSS, 2?% total solids) and 1?% glucose (w/v). Co-polymer production was enhanced from 65-560?mg/l on untreated PSS to 1,610-1,645?mg/l from PSS treated with defined hydrolytic bacteria and 1?% glucose. Supplementation of the PSS hydrolysate with sodium propionate enabled 5BC1 to produce co-polymer P(3HB-co-3HV) with a 3HV content up to 13?% and a concomitant 1.46-fold enhancement in PHA yield. Using the principles of ecobiotechnology, this is the first demonstration of PHA co-polymer production by defined co-cultures of Bacillus from biowaste as feed under non-axenic conditions.</P>

Bioconversion of Glycerol into Copolymers of Polyhydroxyalkanoates and Carotenoids by Paracoccus sp. Strain LL1

Prasun KUMAR,Beom Soo KIM 한국생물공학회 2017 한국생물공학회 학술대회 Vol.2017 No.10

Co-production of polyhydroxyalkanoates and carotenoids through bioconversion of glycerol by <i>Paracoccus</i> sp. strain LL1

Kumar, Prasun,Jun, Hang-Bae,Kim, Beom Soo ELSEVIER 2018 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Vol.107 No.2

<P><B>Abstract</B></P> <P>Polyhydroxyalkanoates (PHA) have emerged as a potential alternative to synthetic plastics, although its large-scale production is limited by the expenditure incurred in feed materials. Co-production of various valuable bioproducts along with PHA has been proposed to alleviate the overall production cost. Here, high-yield co-production of PHA and carotenoids was achieved in single step fermentation by <I>Paracoccus</I> sp. LL1. The halophilic bacterial strain could metabolize a wide range of substrates including methanol, lactose, galactose, glycerol, fructose, mannitol, etc. Under batch fermentation using mineral media supplemented with 2% glycerol, <I>Paracoccus</I> sp. LL1 synthesized 3.77gL<SUP>−1</SUP> PHA with concomitant production of 3.6mgL<SUP>−1</SUP> of carotenoids after 96h. An enhancement of 2.2-folds in total dry cell weight (DCW) was achieved through cell retention culture of <I>Paracoccus</I> sp. LL1, resulting in a maximum DCW (24.2gL<SUP>−1</SUP>) containing PHA (39.3%, ww<SUP>−1</SUP>) with concomitant enhancement in the production of total carotenoids. <I>Paracoccus</I> sp. LL1 could convert glycerol into carotenoids and PHA to a level of 7.14mgL<SUP>−1</SUP> and 9.52gL<SUP>−1</SUP>, respectively. It is the first report showing value addition in PHA production process through co-production of high value carotenoids by <I>Paracoccus</I> sp. using glycerol as substrate.</P>

Genetic Similarity between Cotton Leafroll Dwarf Virus and Chickpea Stunt Disease Associated Virus in India

Arup Kumar Mukherjee,Prasun Kumar Mukherjee,Sandhya Kranthi 한국식물병리학회 2016 Plant Pathology Journal Vol.32 No.6

The cotton leafroll dwarf virus (CLRDV) is one of themost devastating pathogens of cotton. This malady,known as cotton blue disease, is widespread in SouthAmerica where it causes huge crop losses. Recentlythe disease has been reported from India. We noticedoccurrence of cotton blue disease and chickpea stuntdisease in adjoining cotton and chickpea fields and gotinterested in knowing if these two viral diseases havesome association. By genetic studies, we have shownhere that CLRDV is very close to chickpea stunt diseaseassociated virus (CpSDaV). We were successfulin transmitting the CLRDV from cotton to chickpea. Our studies indicate that CpSDaV and CLRDV in Indiaare possibly two different strains of the same virus. These findings would be helpful in managing theseserious diseases by altering the cropping patterns.

Genetic Similarity between Cotton Leafroll Dwarf Virus and Chickpea Stunt Disease Associated Virus in India

Mukherjee, Arup Kumar,Mukherjee, Prasun Kumar,Kranthi, Sandhya The Korean Society of Plant Pathology 2016 Plant Pathology Journal Vol.32 No.6

The cotton leafroll dwarf virus (CLRDV) is one of the most devastating pathogens of cotton. This malady, known as cotton blue disease, is widespread in South America where it causes huge crop losses. Recently the disease has been reported from India. We noticed occurrence of cotton blue disease and chickpea stunt disease in adjoining cotton and chickpea fields and got interested in knowing if these two viral diseases have some association. By genetic studies, we have shown here that CLRDV is very close to chickpea stunt disease associated virus (CpSDaV). We were successful in transmitting the CLRDV from cotton to chickpea. Our studies indicate that CpSDaV and CLRDV in India are possibly two different strains of the same virus. These findings would be helpful in managing these serious diseases by altering the cropping patterns.

Matrix game with Z-numbers

Sibasis Bandyopadhyay,Swapan Raha,Prasun Kumar Nayak 한국지능시스템학회 2015 INTERNATIONAL JOURNAL of FUZZY LOGIC and INTELLIGE Vol.15 No.1

In this paper, a matrix game is considered in which the elements are represented as Z-numbers. The objective is to formalize the human capability for solving decision-making problems in uncertain situations. A ranking method of Z-numbers is proposed and used to define pure and mixed strategies. These strategies are then applied to find the optimal solution to the game problem with an induced pay off matrix using a min max, max min algorithm and the multi-section technique. Numerical examples are given in support of the proposed method.

Diverse Roles of Microbial Indole Derivatives in Eukaryotic Systems

Bharath Reddy BOYA,Prasun KUMAR,Jintae LEE 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

Indole and its derivatives are widespread across different life forms, functioning as signalling molecules in prokaryotes and with more diverse roles in eukaryotes. A majority of indoles found in the environment are attributed to bacterial enzymes converting tryptophan into indole and its derivatives. The involvement of indoles among lower organisms as an interspecies and intraspecies signal is well known, with many reports showing that inter-kingdom interactions involving microbial indole compounds are equally important as they influence defence systems and even the behavior of higher organisms. Here, we discuss the role of microbial indoles in eukaryotes and current perspectives in human diseases and, indole derivatives as biomarkers of metabolic state for therapeutic opportunities.

Evaluation of tomato genotypes for resistance to whitefly (Bemisia tabaci Gennadius) and tomato leaf curl virus in eastern India

Pal Soumita,Karmakar Prasun,Chattopadhyay Arup,Ghosh Sunil Kumar 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.2

Tomato production in the tropics is threatened by whitefly infestation and tomato leaf curl virus (ToLCV) causing severe economic losses. No stable resistance to these biotic challenges has been identified in eastern India. Therefore, initial screening of 19 advance breeding lines of tomato was carried out during the year 2016–17. Based on the whitefly population per leaf and tomato leaf curl disease severity, eight tomato genotypes were selected for final screening during the year 2017–18. Morphological leaf traits and biochemical parameters in tomato leaf were assessed in selected genotypes and considered as potential mediators of resistance. Significant variation was observed for whitefly infestation and ToLCV disease severity among the tomato genotypes tested. Higher leaf trichome density with narrow and thinner leaves and higher total phenol content in leaf emerged as reliable morphological and biochemical markers for early selection of tomato genotype resistant to whitefly and ToLCV disease. The resistance of tomato genotypes 2016/Res-1, 2015/Res-5, 2014/Res-1 and 2014/Res-4 to both whitefly and ToLCV disease is based on antixenotic properties and they could be utilized in future breeding to enhance stable resistance against these biotic challenges.

Matrix Game with Z-numbers

Bandyopadhyay, Sibasis,Raha, Swapan,Nayak, Prasun Kumar Korean Institute of Intelligent Systems 2015 INTERNATIONAL JOURNAL of FUZZY LOGIC and INTELLIGE Vol.15 No.1

In this paper, a matrix game is considered in which the elements are represented as Z-numbers. The objective is to formalize the human capability for solving decision-making problems in uncertain situations. A ranking method of Z-numbers is proposed and used to define pure and mixed strategies. These strategies are then applied to find the optimal solution to the game problem with an induced pay off matrix using a min max, max min algorithm and the multi-section technique. Numerical examples are given in support of the proposed method.