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- 저자 : Ponting, Chris P. (검색결과 28 건)
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Stc1: A Critical Link between RNAi and Chromatin Modification Required for Heterochromatin Integrity
Bayne, Elizabeth H.,White, Sharon A.,Kagansky, Alexander,Bijos, Dominika A.,Sanchez-Pulido, Luis,Hoe, Kwang-Lae,Kim, Dong-Uk,Park, Han-Oh,Ponting, Chris P.,Rappsilber, Juri,Allshire, Robin C. Cell Press 2010 Cell Vol.140 No.5
<P><B>Summary</B></P><P>In fission yeast, RNAi directs heterochromatin formation at centromeres, telomeres, and the mating type locus. Noncoding RNAs transcribed from repeat elements generate siRNAs that are incorporated into the Argonaute-containing RITS complex and direct it to nascent homologous transcripts. This leads to recruitment of the CLRC complex, including the histone methyltransferase Clr4, promoting H3K9 methylation and heterochromatin formation. A key question is what mediates the recruitment of Clr4/CLRC to transcript-bound RITS. We have identified a LIM domain protein, Stc1, that is required for centromeric heterochromatin integrity. Our analyses show that Stc1 is specifically required to establish H3K9 methylation via RNAi, and interacts both with the RNAi effector Ago1, and with the chromatin-modifying CLRC complex. Moreover, tethering Stc1 to a euchromatic locus is sufficient to induce silencing and heterochromatin formation independently of RNAi. We conclude that Stc1 associates with RITS on centromeric transcripts and recruits CLRC, thereby coupling RNAi to chromatin modification.</P>
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Wind velocity field during thunderstorms
Ponte, Jacinto Jr.,Riera, Jorge D. Techno-Press 2007 Wind and Structures, An International Journal (WAS Vol.10 No.3
Wind action is a factor of fundamental importance in the structural design of light or slender constructions. Codes for structural design usually assume that the incident mean wind velocity is parallel to the ground, which constitutes a valid simplification for frequent winds caused by meteorological phenomena such as Extratropical Storms (EPS) or Tropical Storms. Wind effects due to other phenomena, such as thunderstorms, and its combination with EPS winds in so-called squall lines, are simply neglected. In this paper a model that describes the three-dimensional wind velocity field originated from a downburst in a thunderstorm (TS) is proposed. The model is based on a semi empirical representation of an axially-symmetrical flow line pattern that describes a stationary field, modulated by a function that accounts for the evolution of the wind velocity with time. The model allows the generation of a spatially and temporally variable velocity field, which also includes a fluctuating component of the velocity. All parameters employed in the model are related to meteorological variables, which are susceptible of statistical assessment. A background wind is also considered, in order to account for the translational velocity of the thunderstorm, normally due to local wind conditions. When the translation of the TS is caused by an EPS, a squall line is produced, causing the highest wind velocities associated with TS events. The resulting vertical velocity profiles were also studied and compared with existing models, such as the profiles proposed by Vicroy, et al. (1992) and Wood and Kwok (1998). The present model predicts horizontal velocity profiles that depend on the distance to the storm center, effect not considered by previous models, although the various proposals are globally compatible. The model can be applied in any region of interest, once the relevant meteorological variables are known, to simulate the excitation due to TS winds in the design of transmission lines, long-span crossings, cable-stayed bridges, towers or similar structures.
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La muerte enamorada. Amor y muerte en la literatura espa?ola de los siglos XVIII y XIX
Rafael Pontes Velasco 한국스페인어문학회 2005 스페인어문학 Vol.0 No.37
Entre las múltiples preocupaciones que los escritores españoles de los siglos XVIII y XIX abordaron en sus obras, cabe preguntarse por qué elegimos el estudio del "amor" y la "muerte". Sencillamente, porque la conjunción de estos dos conceptos implica una belleza equivalente a la armonía perfecta. Es decir, su unión indisoluble apunta a una suerte de compenetración cósmica, anhelada a lo largo de la historia de la Humanidad. La relación existente entre el amor y la muerte conlleva, en la mayor parte de las letras orientales y occidentales, la asunción de un orden primigenio y, con él, la posibilidad de otra vida fronteriza, anterior o posterior a la perceptible y conocida. Esa otra vida, enlazada íntimamente con la génesis literaria, trataremos de analizarla a través de la semántica profunda de los siguientes textos españoles de los siglos XVIII y XIX: Noches lúgubres (1774), de José Cadalso; Los amantes de Teruel (1836), de Juan Eugenio Hartzenbusch; El estudiante de Salamanca (1839), de José de Espronceda; El diablo mundo (1840), también de José de Espronceda; Don Juan Tenorio (1844), de José Zorrilla; y Rimas (1867), de Gustavo Adolfo Bécquer. El comentario de estas obras no se detendrá en el género literario de las mismas ni en sus peculiaridades formales. En cambio, adoptaremos una óptica centrada en los hallazgos de algunos de los libros más radicales a la hora de afrontar la comunión del amor y la muerte en la literatura, como Justine o los infortunios de la virtud (1791), del Marqués de Sade; Más allá del principio del placer (1920), de Sigmund Freud; El erotismo (1957), de Georges Bataille; y Pensamiento y cultura en la antigua India (1995), de Ana Agud.
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El cine, Cagliostro y la generacin del 27
Rafael Pontes Velasco 한국스페인어문학회 2007 스페인어문학 Vol.0 No.45
Cagliostro (1934), “novela visual” del poeta Vicente Huidobro, se relaciona con la generación del 27 en tanto asume y personaliza los postulados filosóficos que los artistas de la época mantenían en torno al cine y la literatura. Anticipadamente, el principal promotor del creacionismo apoyó la consolidación del interés de La Gaceta literaria por el séptimo arte. El propósito de nuestro artículo reside en reconstruir el puente que Cagliostro pudo haber diseñado entre Huidobro y los autores españoles del 27, propiciado por una inspiración vanguardista común. Estructuramos nuestra reflexión en cuatro partes. En primer lugar, proponemos una sinopsis argumental de Cagliostro y un comentario del mismo, a la luz de varios hallazgos estéticos de Luis Buñuel, Salvador Dalí, Federico García Lorca y Rafael Alberti. A continuación, analizamos algunas de las técnicas cinematográficas de la novela, teniendo en cuenta sus procedimientos literarios. En tercer lugar, realizamos una digresión interpretativa en torno al simbolismo de “los ojos” en el cine, y por tanto en la literatura de Huidobro y la generación del 27. Finalmente, damos paso a las conclusiones valorativas y señalamos el posible alcance profético que la vinculación primigenia entre la magia y la imagen suscitaba en los primeros artistas de vanguardia.
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Wind velocity field during thunderstorms
Jacinto Ponte Jr.,Jorge D. Riera 한국풍공학회 2007 Wind and Structures, An International Journal (WAS Vol.10 No.3
Wind action is a factor of fundamental importance in the structural design of light or slender constructions. Codes for structural design usually assume that the incident mean wind velocity is parallel to the ground, which constitutes a valid simplification for frequent winds caused by meteorological phenomena such as Extratropical Storms (EPS) or Tropical Storms. Wind effects due to other phenomena, such as thunderstorms, and its combination with EPS winds in so-called squall lines, are simply neglected. In this paper a model that describes the three-dimensional wind velocity field originated from a downburst in a thunderstorm (TS) is proposed. The model is based on a semi empirical representation of an axially-symmetrical flow line pattern that describes a stationary field, modulated by a function that accounts for the evolution of the wind velocity with time. The model allows the generation of a spatially and temporally variable velocity field, which also includes a fluctuating component of the velocity. All parameters employed in the model are related to meteorological variables, which are susceptible of statistical assessment. A background wind is also considered, in order to account for the translational velocity of the thunderstorm, normally due to local wind conditions. When the translation of the TS is caused by an EPS, a squall line is produced, causing the highest wind velocities associated with TS events. The resulting vertical velocity profiles were also studied and compared with existing models, such as the profiles proposed by Vicroy, et al. (1992) and Wood and Kwok (1998). The present model predicts horizontal velocity profiles that depend on the distance to the storm center, effect not considered by previous models, although the various proposals are globally compatible. The model can be applied in any region of interest, once the relevant meteorological variables are known, to simulate the excitation due to TS winds in the design of transmission lines, long-span crossings, cable-stayed bridges, towers or similar structures.
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Al filo de Guillermo Samperio: un escritor inclasificable
( Rafael Pontes Velasco ) 고려대학교 스페인·라틴아메리카연구소 2008 스페인라틴아메리카연구 Vol.1 No.-
El proposito de este articulo reside en una doble vertiente de difusion y analisis. Por una parte, presentaremos a Guillermo Samperio, uno de los escritores mexicanos de mayor trascendencia y posibilidades de perduracion en la literatura latinoamericana contemporanea. Por otra, trazaremos pinceladas criticas sobre su obra, una de las mas arriesgadas en el aspecto formal e innovadoras en cuanto a tematica de los ultimos tiempos, desde una perspectiva cronologica y desde diferentes generos literarios. Asumiremos para ello una metodologia que combine el rigor filologico con la aceptacion de las dificultades implicitas que supone abordar lo inclasificable de una apuesta unica y en continuo crecimiento, con la conviccion de que la indudable calidad de la creacion de Samperio merece un puesto destacado y memorable en la historia literaria de nuestra era. El presente estudio abarcara desde el primer libro de cuentos de nuestro autor, Cuando el tacto toma la palabra (1974), hasta el ultimohasta la fecha, La guerra oculta (2008), revisando el resto de titulos significativos de una amplia trayectoria en narrativa, lirica y ensayistica. Dada la versatil y ya extensa bibliografia samperiana, procuraremos aportar una vision global de una propuesta tan brillante en la escritura de largo aliento como maestra y definitoria en los marcos de la ficcion breve. Bienvenidos al fascinante mundo de Guillermo Samperio, del que resulta tan accesible su entrada como imposible su salida.
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