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Tomatotone과 Gibberellin(GA3)의 處理가 輸出用 가지(Solanum melongena. L)의 生育 및 收量에 미치는 影響
최성관,이재필,진동호,오주성,박흥식,정순재 동아대학교 농업생명과학연구소 2001 農業生命資援硏究 Vol.10 No.1
These grafting seeding "Torobambica", rootstock "Jang Ja" and "Chuk Yang" were tested to investigate the differences between the growth of eggplants and the number of eggplants which can be influenced by the growth retardant aerosol. For the test of the growth retardant aerosol, the flower clusters were sprayed with one hundred units of Tomatotone liquid and with three hundred units of GA3 on the day they flowered and sprayed two or three days before flowering. The results were as follows: 1.According to the growth retardant aerosol of Tomatotone, the plant height was the tallest at 226.21 cm with foliar spray and the high Marketable fruit was good at the percent of 77.38 with a flowering spray and Tomatotone on the day they flowered. 2.According to the growth retardant aerosol of GA3, the plant's height was the tallest at 229.27cm with foliage which was sprayed every week. The high quality fruit was good at the percent of 69.11 with a flowering spray and GA3 on the day they flowered. 3.According to Tomatotone and GA3 treatments of the plant growth regulators, the plant's height was the tallest sprayed every week and the high Marketable fruit was good at the flowering spray and Tomatotone on the day they flowered. and Yield per 10a was good at the flowering spray and Tomatotone on the day they flowered.
Sperm Fertility and In Vitro Developmental Potential of Jeju Black Cattle Bull and Hanwoo Bull
Jae-Wook Yoon,Seung-Eun Lee,Hyo-Jin Park,Sang-Gi Jeong,Won-Jae Kim,Chan-Oh Park,Dong-Yong Hong,Min-Jee Park,Eun-Young Kim,Se-Pill Park 한국동물생명공학회(구 한국동물번식학회) 2018 발생공학 국제심포지엄 및 학술대회 Vol.2018 No.06
The Jeju Black Cattle (JBC) are a type of traditional Korean native cattle with a characteristic black fur that covers the entire body. The breed’s qualities and genetic uniqueness helped it to be named as a protected, state-designated national monument in 2013. However, they are endangered crisis. Therefore, in vitro fertilization (IVF) technology is needed to preserve these endangered animals and to further improve their traits. In this study, we compared the sperm fertility and developmental potential of IVF embryos using Hanwoo sperm supplied by Korea Animal Improvement Association and JBC sperm made by Jeju Special Self-Governing Province Livestock bull semen Promotion Institute. [Hanwoo A, B (KPN) and JBC A, B grade]. Sperm was inseminated in 44 μL IVF drop contained 10 oocytes with sperm concentration of 1×106 cells/mL, and then 2 μL heparin and 2 μL PHE (20 μM penicillamine, 10 μM hypotaurine, 2 μM epinephrine) were added. At 2 days after IVF, the in vitro fertilization rate was significantly higher in Hanwoo-B sperm group (82.3±1.1) than in JBC-A sperm group (64.6±2.3) (p<0.05). The highest percentage of blastocyst formation was produced in Hanwoo-B sperm group (43.0±3.9) among four treatment sperm groups. The trophectoderm (TE) cell number was higher in Hanwoo-A sperm group (122±7.8) than in JBC-A sperm group (73±12.6). The inner cell mass (ICM) cell number of Hanwoo-A sperm group (46±2.5) was similar to that of JBC-A sperm group (46±4.0). Thus, the total cell number of day 8 blastocyst was higher in Hanwoo-A sperm group (168±8.9) than in JBC-A sperm group (119±15.9). These results demonstrate that there were different in sperm quality (viability and motility) between Hanwoo sperm (60∼70%) and JBC sperm (50∼60%), and their in vitro development capacity was also presented as difference. We need to embryo transfer study for field trial.
Park, Jae Hoon,Kim, Sang Bum,Lee, Eung Pill,Lee, Seung Yeon,Kim, Eui Joo,Lee, Jung Min,Park, Jin Hee,Cho, Kyu Tae,Jeong, Heon Mo,Choi, Seung Se,Park, Hoey Kyung,You, Young Han The Ecological Society of Korea 2021 Journal of Ecology and Environment Vol.45 No.1
Background: The sunfleck is an important light environmental factor for plants that live under the shade of trees. Currently, the smartfarm has a system that can artificially create these sunfleks. Therefore, it was intended to find optimal light conditions by measuring and analyzing photosynthetic responses of Eutrema japonica (Miq.) Koidz., a plant living in shade with high economic value under artificial sunflecks. Results: For this purpose, we used LED pulsed light as the simulated sunflecks and set the light frequency levels of six chambers to 20 Hz, 60 Hz, 180 Hz, 540 Hz, 1620 Hz, and 4860 Hz of a pulsed LED grow system in a plant factory and the duty ratio of the all chambers was set to 30%, 50%, and 70% every 2 weeks. We measured the photosynthetic rate, transpiration rate, stomatal conductance, and substomatal CO2 partial pressure of E. japonica under each light condition. We also calculated the results of measurement, A/Ci, and water use efficiency. According to our results, the photosynthetic rate was not different among different duty ratios, the transpiration rate was higher at the duty ratio of 70% than 30% and 50%, and stomatal conductance was higher at 50% and 70% than at 30%. In addition, the substomatal CO2 partial pressure was higher at the duty ratio of 50% than 30% and 70%, and A/Ci was higher at 30% than 50% and 70%. Water use efficiency was higher at 30% and 50% than at 70%. While the transpiration rate and stomatal conductance generally tended to become higher as the frequency level decreased, other physiological items did not change with different frequency levels. Conclusions: Our results showed that 30% and 50% duty ratios could be better in the cultivation of E. japonica due to suffering from water stress as well as light stress in environments with the 70% duty ratio by decreasing water use efficiency. These results suggest that E. japonica is adapted under the light environment with nature sunflecks around 30-50% duty ratio and low light frequency around 20 Hz.
Evaluation of Jeju Black Cattle Bull Sperm
Jae-Wook Yoon,Seung-Eun Lee,Won-Jae Kim,Sang-Gi Jeong,Chan-Oh Park,Hyo-Jin Park,Dong-Yong Hong,Min-Jee Park,Eun-Young Kim,Se-Pill Park 한국동물생명공학회(구 한국동물번식학회) 2018 발생공학 국제심포지엄 및 학술대회 Vol.2018 No.06
The Jeju Black Cattle (JBC) are a type of traditional Korean native cattle with a characteristic black fur that covers the entire body. Jeju black cattle are smaller than other breeds and their disposition is very calm. The breed’s qualities and genetic uniqueness helped it to be named as a protected, state-designated national monument in 2013. However, they are endangered crisis. Therefore, in vitro fertilization (IVF) technology is needed to preserve these endangered animals and to further improve their traits. In this context, semen analysis is the most commonly used procedure to evaluate male fertility potential in humans and animals. This study was performed on 3 Jeju Black Cattle (JBC) Bulls belonging to Jeju Special Self-Governing Province Promotion Institute. [JBC A, B, C grade]. The freezing medium (20% egg yolk plus 20% triladyl) to a final concentration of 100×106 sperm cells/mL. For sperm cooling, the 0.5 mL plastic straws filled with diluted samples were kept in refrigerator at 4°C for 2 h. They were placed 3 or 7 cm over liquid nitrogen (LN2) vapor for 10 or 30 min and then directly plunged into LN2 for storage. Thawing was done by transferring the frozen straws into water bath at 37°C for 30 sec for analysis. The sperm motility in each group was assessed using the Sperm Analysis Imaging System (SAIS Plus; Medical Supply Co, Ltd., Korea). The sperm vitality in each group was assessed using the eosin-nigrosin stain. The sperm morphology in each group was assessed using the diff-quik kit. The motility was significantly lower in JBC-C frozen-thawing sperm group (35∼65%) than in JBC-C fresh sperm group (98.5%) (p<0.05). The vitality of fresh sperm group (45∼61%) was similar to that of frozen-thawed sperm group (30∼65%). The morphology of fresh sperm group (72∼89%) was similar to that of frozen-thawed sperm group (83∼93%). These results demonstrate that there were similar in sperm quality (vitality and motility) between fresh sperm and frozen-thawed sperm.
Supplementation of Icariin Protects Porcine Oocyte from Deterioration during In Vitro Aging
Jae-Wook Yoon,Seung-Eun Lee,Min-Jee Park,Won-Jae Kim,Chan-Oh Park,Hyo-Jin Park,Eun-Young Kim,Se-Pill Park 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11
Preimplantation embryonic production in vitro is important in human assisted reproductive technology and animal embryo engineering. Icariin (ICA) is one type of flavonoids and a main component isolated from the stem leaf of Epimedium brevicornum. Flavonoids, which are among the best well-studied natural antioxidants, have been demonstrated to be active in clearing reactive oxygen species (ROS). The purpose of this study was to investigate the effects of ICA treatment during porcine oocyte in vitro aging and their in vitro developmental competency after parthenogenetic activation (PA). Porcine oocytes were matured in vitro for 44 h (control) and for an additional 24 h in the presence of 0, 5 μM ICA (aging, ICA-5), respectively. This study investigated the effects of ICA on nuclear maturation, ROS level, apoptosis index, and the developmental capacity of aged porcine oocytes. Oocyte survival was not different in aging group compared to control or ICA-5 group. The increased ROS level during in vitro aging was prevented in ICA-5 group, while GSH level was not decreased. The decrease of normal spindle formation during in vitro aging was prevented in ICA-5 group. After PA, although the cleavage rate was not different among treatment groups, the blastocyst formation was significantly higher in control and ICA-5 group than in aging group. However, there was significantly difference in the apoptotic index of the ICA-5 group, while it was no difference in the total cell number of the ICA-5 group. (p<0.05). Therefore, this result demonstrated that the ICA is an effective agent to prevent the deterioration during in vitro aging of porcine oocytes.
Production of Cloned Jeju Black Cattle by Somatic Cell Nuclear Transfer and Flavonoid Treatment
Jae Youn Kim,Kyoung Ha So,Min Jee Park,Hyo Young Park,Eun Hyung Noh,Eun Ji Noh,Seong Ho Mun,Young Hoon Kim,Sung Soo Lee,Moon Suck Ko,Eun Young Kim,Se Pill Park 한국동물생명공학회(구 한국동물번식학회) 2009 발생공학 국제심포지엄 및 학술대회 Vol.2009 No.1