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        Determination of the energy contents and nutrient digestibility of corn, waxy corn and steam-flaked corn fed to growing pigs

        Dongli Ma,Juntao Li,Chengfei Huang,Fengjuan Yang,Yi Wu,Ling Liu,Wei Jiang,Zhicheng Jia,Peijun Zhang,Xuezhen Liu,Shuai Zhang 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.10

        Objective: The research was conducted to determine the digestible (DE) and metabolizable energy (ME) contents as well as the apparent total tract digestibility (ATTD) of nutrients in corn, waxy corn and steam-flaked corn fed to growing pigs. Methods: Eighteen growing pigs with initial body weight of 15.42±1.41 kg were randomly allotted to three diets including a corn diet, a waxy corn diet and a steam-flaked corn diet in a completely randomized design. Each treatment contained six replicates. The experiment lasted for 12 days, which comprised 7-d adaptation to diets followed by a 5-d total collection of feces and urine. The energy contents and the nutrient digestibility in three ingredients were calculated using direct method. Results: Compared to normal corn, both the amylose and dietary fiber contents in waxy corn were numerically lower, but the starch gelatinization degree was numerically greater. Moreover, the DE and ME contents as well as the ATTD of neutral detergent fiber and acid detergent fiber (ADF) in waxy corn were significantly greater (p<0.05) than those in normal corn when fed to growing pigs. Furthermore, the steam-flaked corn had greater (p<0.05) DE and ME contents, and ATTD of ether extract and ADF compared to normal corn. Conclusion: Both variety and processing procedure have influence on chemical compositions, energy contents and nutrient digestibility of corn. The waxy corn and steam-flaked corn had greater degree of starch gelatinization and DE and ME contents compared to normal corn when fed to growing pigs.

      • A numerical investigation of the tensile behavior of the thread-fixed one-side bolted T-stubs at high temperature

        Yang You,Le Liu,Xiaojing Huang,Peijun Wang,Fangzhou Liu 국제구조공학회 2022 Steel and Composite Structures, An International J Vol.45 No.4

        The tensile behavior of the Thread-fixed One-side Bolt (TOB) at high temperatures was studied using the Finite Element Modeling (FEM) to explore the structural responses that could not be measured in tests. The accuracy of the FEM was verified using the test results from the failure mode, load-displacement curve as well as yielding load. Three typical failure modes of TOB connected T-stubs were observed, which were the Flange Yielding (FY), the Bolt Failure (BF) and the Coupling Failure mode (CF). The influence of the flange thickness tb and the temperature θ on the tensile behavior of the T-stub were discussed. The initial stiffness and the yielding load decreased with the increase of the temperature. The T-stubs almost lost their resistance when the temperature exceeded 700℃. The failure modes of T-stubs were mainly decided by the flange thickness, which relates to the anchorage of the hole threads and the bending resistance of flange. The failure mode could also be changed by the high temperature. Design equations in EN 1993-1-8 were modified and verified by the FEM results. The results showed that these equations could predict the failure mode and the yielding load at different temperatures with satisfactory accuracy.

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        Development and Evaluation of a Duplex Real-Time PCR Assay With a Novel Internal Standard for Precise Quantification of Plasma DNA

        Dan Chen,Shi-yang Pan,Erfu Xie,Li Gao,Huaguo Xu,Wenying Xia,Ting Xu,Peijun Huang 대한진단검사의학회 2017 Annals of Laboratory Medicine Vol.37 No.1

        Background: Circulating levels of cell-free DNA increase in many pathologic conditions. However, notable discrepancies in the quantitative analysis of cell-free DNA from a large number of laboratories have become a considerable pitfall, hampering its clinical application. Methods: We designed a novel recombinant DNA fragment that could be applied as an internal standard in a newly developed and validated duplex real-time PCR assay for the quantitative analysis of total cell-free plasma DNA, which was tested in 5,442 healthy adults and 200 trauma patients. Results: Compared with two traditional methods, this novel assay showed a lower detection limit of 0.1 ng/mL, lower intra- and inter-assay CVs, and higher accuracy in the recovery test. The median plasma DNA concentration of healthy males (20.3 ng/mL, n=3,092) was significantly higher than that of healthy females (16.1 ng/mL, n=2,350) (Mann-Whitney two-sample rank sum test, P<0.0001). The reference intervals of plasma DNA concentration were 0-45.8 ng/mL and 0-52.5 ng/mL for healthy females and males, respectively. The plasma DNA concentrations of the majority of trauma patients (96%) were higher than the upper normal cutoff values and were closely related to the corresponding injury severity scores (R2=0.916, P<0.0001). Conclusions: This duplex real-time PCR assay with a new internal standard could eliminate variation and allow for more sensitive, repeatable, accurate, and stable quantitative measurements of plasma DNA, showing promising application in clinical diagnosis.

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