S . L . E . 환자 혈액에서 분리한 Listeria monocytogenes 의 세균학적 특성

김혜숙,정윤섭,이삼열 대한미생물학회 1973 大韓微生物學會誌 Vol.8 No.1

Listeria monocytogenes human infection is a relatively rare disease which usually is meningitis in newborn babies. The organism was isolated from blood cultures of a 52 year old female patient with meningitis. It was considered that the underlying disease, I.e. S.L.E., and the steroid therapy which the patient had been receiving played some role for the Listeria infection. The isoIate was showing characteristics of L. monocytogenes, I.e. diphtheroid like morphology, motility with four peritrichous flagella, hemolytic small colonies on blood agar, growth in the presence of 7. 5% salt and at 4 C, and inducing monocytosis in an experimentally infected rabbit. Serologically the organism was identified as L. monocytogenes 4b. The isolate was showing susceptibility to many antibiotics tested including ampicillin, penicillin and tetracycline which were the recommended drugs of choice for the treatment of Listeriosis. It is the general opinion that Listeriosis is not so rare as literatures are showing. It is considered that some of the isolate of the organism from clinical specimens are mistakenly discarded due to the fact that the organism shows diphtheroid like morphology and that not many laboratories are able to recognize the organism. Literatures are seen which emphasize more careful examination of gram positive bacilli with diphtheroid like morphology especially when they are isolated from blood or from spinal fluid of patient.

혈액에서 혐기성 세균이 분리된 환자의 임상 및 세균학적 검토

정윤섭,이삼열,김진주 대한미생물학회 1985 大韓微生物學會誌 Vol.20 No.1

Isolation and identification of anaerabic bacteria fmm blood cultures are still technically demanding procedures. Recently, with thq use of gas hquid chmmatogWphy, the accuracy of identification is much improved, However, there has niver been a satisfactory data apalysis on anaerobic bacteremia in Korea. The authors evaluateid both the chnical and the bacteriological 4ata of 129 anaerobic bacteremias found at the Yonsei Medidal Center during the period of 1973 to 1984. The most frequently isolated anaerobic bacteria were Bacteroides (52.7%), among which the major spjcies was B. fragilis (38.7%) . Incidence of anae- robic bacterethia by sex was 57% in male and 43% in female. Mortality was high in groups below 1-year old and abom 50-year old. The cause of death seemed qlosely correlated with the patients age, general condition and the s!.verity of the underlying disease. Various neoplasms were the most common (20%) un- derlying disesses pxedisposing the anaerobic bacteremia. Biliary tract was considered the most frequent route of infection in anaetobic bacteremia. The frequent clinic8l signs in anaerobic bactetemia were fever (65%), followed by liver function abnormality (29%), jaundice (20%) and hypotention (18%) . When analysis of positive rate,of blood cutture was made on the patients from whom 4 cultures were done within 24 houm. It was found that 33% of the samples were positive. Isolation rate of anaembic bacteria in thioglycollate medium was 83.8%, while it was 44% in Tryptic soy broth. Among the anaerobic bacteremia, 25.4% were polymicrobia1 infections with aerobic bacteria (92.5%), such as E. Coli(33.3%). From these studies, it is con- cluded that h. fragilis is the most important causative organism in anaerobic bacteremia, with high fatality, particularly ih those who have underlying diseases. The ports of entry me mainly biliary, gastrointestinal and fernale genital traet. Fever is the most frequent clinical sign. Single blood culture is not sufficient to detect all anaerobic bacteremia, therefore more cultures with optimal time interval are needed. The incidence of polymicrohial infection in anaerobic bacteremia is higher than that in overall bacteremia.

장염환자에서의 Campylobacter fetus subsp . jejuni 분리율

이귀녕,정윤섭,이삼열 대한미생물학회 1982 大韓微生物學會誌 Vol.17 No.1

C. fefus subsp. Jejuni has been reported to be an important enteric pa.thogen in many parts of the world. Although the infection has been reparted in Korea, the incidence is not known. In this :study the reaults of stool culture during the period oi August 1981 to July 1982 at Yonsei Medical Center was ana.lyzed and the foliowing resuits were obtaioed. 1. C. fetus subsp. Jejuni was isolated from 0. 8/a of stool specimens. The isolation rate was lower than that of salmonella (3.3%) and shigella (7.1%). The isolation was most frequent in June and from 15-year-old patients. 2. All of the isola.tes from the patients were susceptible to chloramphenicol aod erythromycin. It was noteworthy that 4 isolates were resistant to all of the aminoglycosides, I,e., amikacin, gen- tamicin, kanamycin and tobramycin. 3. We also isolated C. fetus subsp. Jejuni iram chicken. When the susceptibility of the isolates was compared to that of tbe isolates from human the former were less susceptible to erythro- myein (34.1%) and tetracycline (38.6%).

Sodium Amylosulfate 의 Salmonella typhi 증식에 대한 영향

김성옥,정윤섭,이삼열 대한미생물학회 1976 大韓微生物學會誌 Vol.11 No.1

Sodium amylosulfate (SAS) has been reported to be an effective substance to inactivate the anti- bacterial activity of blood in bIood cuIture media. The advantage of the use of SAS over sodium polyanethol suIfonate (SPS) is that it does not inhibit (he growth of some bacterial species whir,h are known to be inhibited by SPS. As to S. typhi, SPS is reported to enhance the growth, however the effect of SAS on this or ganism is not known as yet. Using 43 strains of S. typhi, isotated from clinical materials, the authors tried to determine the effect of SAS on this organism. Tbe methods used for this study were: the SPS and SAS paper disk sensitivity test, tests on the growth in trypticase soy broth (TSB) with SPS and with SAS, and experimental blood culture in SPS and SAS incorporated TSB. The following results were obtined. 1). S. typhi strains with the turbidity of No. 0. 5 tube of MacFarland nepherometer were inoculated onto Mueller-Hinton plate and 1 mg disk of SPS and SAS were applied. After 24-hour incubation, none of the 43 strains showed inhibition zone by SPS disk, but all of them showed zones by SAS disk with a mean zone diameter of 9.5 mm (Table 1). 2) Inocula consisting of one to 54 viabIe counts of 37 strains wee inoculated into three different media; TSB with 0. 05% SPS, TSB with 0. 05% SAS and TSB alone. After 24-hour incubation the mean of the optical densities of each medium were 0. 483, 0 482 and 0. 459 respectively, showing that SAS does not inhibit the growth of S. typhi. Moreover it was shown that there was no correlation between the amount of inocuIa and growth (Table 2 and Fig. 1). 3), Each set of media in 5 ml amounts consisting of one tube of TSB with 0. 05% SPS, one tube of TSB with 0,05% SAS and two tubes of TSB were inoculated with 8, 64.640 and 6400 viable counts of bacteria. Then 0. 5 ml of fresh normal blood was added to all tubes except for one tube of TSB. Macroscopic observation after 24 hour incubation showed a heavy growth in all tubes except for the tube of TSB plus blood, which showed only a light growth in the tube of the heaviest inoculum. This result clearly demonstrates that the growth of S. typhi is inhibited by some antibacterial activities of fresh bIood, which are counter acted by SPS and SAS (Table 3). Between SPS and SAS, there was no significant difference found (Table 4 and Fig. 2). With all these resulta it can be postulated that the addition of SAS into a rountine blood culture media. May raise the positivity of S. typhi isolation and shorten the incubation period.

Vibrio vulnificus 분리율에 대한 SPS Agar와 SGP Broth의 사용 및 검체 저장의 영향

정윤섭,이삼열,김신무 대한미생물학회 1987 大韓微生物學會誌 Vol.22 No.2

Vihrio vulnificus septicemia is not-rare dicase in Korea. Carriage rate of the orgaisrn by shellfish is not well known. In this study performance of SPS agar and SGP hroth ancl effect of storage of specimen in the isolaton was determined using the shellfish specimens collected from the coast and market of Koonsan city. Isolation rate was similar with TCBS and with SPS, but the rate became much highher after enrichment in SGP broth. 80% of oyster speirnes were positve when inoculatecJ immediately, but the rate dropped rapidly after storage of specirnens at freezing t.emperature for sornetime. All of the isolates fermented lactose in 2 days. A few isolates were not identfiable with API 20E system, because of acid prduction from melibiose. Serover 04 was the frequent isolates.

혁명론자와 진화론자

알렌,박정민 한국음악사학회 1994 韓國音樂史學報 Vol.13 No.1

음악사는 ' 주체 ' 를 가지는가 ?

달하우스,박종문 한국음악사학회 1991 韓國音樂史學報 Vol.7 No.1

수용사의 문제점들

달하우스,김종희 한국음악사학회 1993 韓國音樂史學報 Vol.10 No.1

임상검사물에서의 혐기성세균 분리

권오헌,정윤섭,이삼열 대한미생물학회 1975 大韓微生物學會誌 Vol.10 No.1

Anaerobic bacteria are the major residents of human skin and mucous membrane. The importance, as opportunistic pathogens, of anaerobic bacteria are well recognized because of more population. With decreased defense against bacterial invasion due to chemotherapy, rediation therapy, extensive surgical operation etc. are dealt at hospitals. An analysis of the anaerobe isolation results at Yonsei University Medical Center during the January 1974-May 1975 period was made and the following results were obtained. 1) From 118 patients 146 strains of anaerobes were isolated. Among these 81.3% were nonsporforming anaerobes. Most frequentIy isoIated anaerobes were Pc. Asaccharolyticus, Ps. Anaerobius, Ps. Intermedius, B. fragilis and Cl. Perfriegens. 2) Anaerobes were frequentIy isolated from wound, female genital, intraabdominal, and pleuropulmonary specimens. Fewer anaerobes were isolated from blood, spinal fluid and liver specimens. 3) The ratio of anaerobe isolation to total bacteria isolation were; liver 66. 7%, intraabdominal 33, 3% pleuropulmonary 28. 9%, spinal fluid 5,0% and bIood 4. 2%. 4) Among the 118 anaerobe isolated patients, 48. 3% yielded anaerobes only and rest of them yielded anaerobes together with aerobes. 5) Most of the gram-positive anaerobes were susceptible to the antibiotics tested. Exception was to tetracycline to which appreciable number showed resistance. It was notewarthy that only 48% of B. fragilis was susceptibIe to tetracycline.

임상검체에서의 Haemophilus aphrophilus 분리 5예

김태숙,정윤섭,이삼열,설준희,양성익,이의웅 대한미생물학회 1985 大韓微生物學會誌 Vol.20 No.1

Haemophilus aphrophilus is a fastidious gram-negative bacillus found in mouth of normal individuals. Though H. aphrophilus infection is quite rate, it includes such serious ones as endocarditis and brain abscess. The authom isolated H. aphrophilus from five patients with the diagnosis of lung abscess, conjunctivitis, brain abscess and facial masticator space abscaa Two of the patients died. Three of the patients also yielded other species of bacteria from the same specimens. One of the isolate was intermediately susceptible to amikacin and resistant to tobramycin, indicating the necessity of a routine susceptibility test in order to select the proper antimicrobial agents. Since FS. Aphrophilus can be diffetentiated from other similar organisms by morphological and biochemical chamcteristics, one should determie the possibility of this organisrn when faptidious gram-negative bacilli are isolated from blood or from sites adjacent to upper respiratory tract.