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Inhibition of Human Hemoglobin Autoxidaiton by Sodium n-Dodecyl Sulphate
Reza, Dayer Mohammad,Ali Akbar, Moosavi-Movahedi,Parviz, Norouzi,Ghourchian, Ghourchian,Hedayat-Olah, Hedayat-Olah,Shahrokh, Safarian 생화학분자생물학회 2002 Journal of biochemistry and molecular biology Vol.35 No.4
The effect of sodium n-dodecyl sulphate (SDS) on hemoglobin autoxidation was studied in the presence of a 100mM phosphate buffer (pH 7.0) by different methods. These included spectorphotometry, fluorescence technique, cyclic voltametry, differential scanning calorimetry, and densitometry. Spectroscopic studies showed that SDS concentrations up to 1 mM increased deoxy-, decreases oxy-, and had no significant effect on the met- conformation of hemoglobin. Therefore, a SDS concentration up to 1 mM increased the deoxy form of hemoglobin as the folded, compact state and decreases the oxy conformation. The turbidity measurements and differential scanning calorimetry techniques indicated a more stable conformation for hemoglobin in the presence of SDS up to 1mM. Electrochemical studies also confirmed a more difficult oxidation under these conditions. The induction of the deoxy form in the presence of SDS was confirmed by densitometry techniques. The compact structure of deoxyhemoglobin blocks the formation of met-conformation in low SDS concentrations.
ON CERTAIN IDENTITIES FOR MEANS, III
J. Sandor,E. Egri,R. Olah-Gal 장전수학회 2009 Advanced Studies in Contemporary Mathematics Vol.19 No.1
We offer certain in¯nite series representations of general integral means. As applications, we can reobtain in an unitary way, many known or new identities for special means, thus continuing and complementing results from parts I and II (see [9], [12]). As corollaries, many inequalities involving means are pointed out.
McGrath, Kimberly M.,Prakash, G. K.Surya,Olah, George. A. 한국공업화학회 2004 Journal of Industrial and Engineering Chemistry Vol.10 No.7
Over the past 15 years, the electrical performance of direct methanol fuel cells (DMFCs) has increased at least tenfold. With this advancement, portable DMFC-powered devices have come to fruition along with dramatic improvement in small power plant and transportation applications. While the DMFC has a theoretical energy density of 6094 W h/kg, only 1000-3200 W h/kg of this energy is realized in practice. Although most portable power applications only necessitate several W h/kg, performance and cost issues associated with polymer electrolyte membranes (PEMs), anode and cathode catalysts, system design, and operational considerations must be overcome for DMFCs to have a role in the methanol economy. This review covers mostly the chemistry aspects of DMFCs.
Inhibition of Human Hemoglobin Autoxidation by Sodium n - Dodecyl Sulphate
(Dayer Mohammad Reza),(Moosavi Movahedi Ali Akbar),(Norouzi Parviz),(Ghourchian),(Hedayat Olah),(Safarian Shahrokh) 생화학분자생물학회 2002 BMB Reports Vol.35 No.4
The effect of sodium n-dodecyl sulphate (SDS) on hemoglobin autoxidation was studied in the presence of a 100 mM phosphate buffer (pH 7.0) by different methods. These included spectrophotometry, fluorescence technique, cyclic voltametry, differential scanning calorimetry, and densitometry. Spectroscopic studies showed that SDS concentrations up to 1 mM increased deoxy-, decreases oxy-, and had no significant effect on the met- conformation of hemoglobin. Therefore, a SDS concentration up to 1 mM increased the deoxy form of hemoglobin as the folded, compact state and decreases the oxy conformation. The turbidity measurements and differential scanning calorimetry techniques indicated a more stable conformation for hemoglobin in the presence of SDS up to 1 mM. Electrochemical studies also confirmed a more difficult oxidation under these conditions. The induction of the deoxy form in the presence of SDS was confirmed by densitometry techniques. The compact structure of deoxyhemoglobin blocks the formation of met-conformation in low SDS concentrations.
Bioreactor Conditioning of Valve Scaffolds Seeded Internally with Adult Stem Cells
Allison Kennamer,Leslie Sierad,Richard Pascal,Nicholas Rierson,Christopher Albers,Marius Harpa,Ovidiu Cotoi,Lucian Harceaga,Peter Olah,Preda Terezia,Agneta Simionescu,Dan Simionescu 한국조직공학과 재생의학회 2016 조직공학과 재생의학 Vol.13 No.5
The goal of this study was to test the hypothesis that stem cells, as a response to valve-specific extracellular matrix “niches” and mechanical stimuli, would differentiate into valvular interstitial cells (VICs). Porcine aortic root scaffolds were prepared by decellularization. After verifying that roots exhibited adequate hemodynamics in vitro, we seeded human adipose-derived stem cells (hADSCs) within the interstitium of the cusps and subjected the valves to in vitro pulsatile bioreactor testing in pulmonary pressures and flow conditions. As controls we incubated cell-seeded valves in a rotator device which allowed fluid to flow through the valves ensuring gas and nutrient exchange without subjecting the cusps to significant stress. After 24 days of conditioning, valves were analyzed for cell phenotype using immunohistochemistry for vimentin, alpha-smooth muscle cell actin (SMA) and prolyl-hydroxylase (PHA). Fresh native valves were used as immunohistochemistry controls. Analysis of bioreactor-conditioned valves showed that almost all seeded cells had died and large islands of cell debris were found within each cusp. Remnants of cells were positive for vimentin. Cell seeded controls, which were only rotated slowly to ensure gas and nutrient exchange, maintained about 50% of cells alive; these cells were positive for vimentin and negative for alpha-SMA and PHA, similar to native VICs. These results highlight for the first time the extreme vulnerability of hADSCs to valve-specific mechanical forces and also suggest that careful, progressive mechanical adaptation to valve-specific forces might encourage stem cell differentiation towards the VIC phenotype.