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- 저자 : Ohme-Takagi, Masaru (검색결과 2 건)
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HB31 and HB21 regulate floral architecture through miRNA396/GRF modules in Arabidopsis
Lee Young Koung,Olson Andrew,김근화,Ohme-Takagi Masaru,Ware Doreen 한국식물생명공학회 2024 Plant biotechnology reports Vol.18 No.1
Floral architecture plays a pivotal role in developmental processes under genetic regulation and is also influenced by envi- ronmental cues. This affects the plant silique phenotype in Arabidopsis and grain yield in crops. Despite the relatively small number of family members of zinc finger homeodomain (ZF-HD) transcription factors (TFs) in plants, their biological role needs to be investigated to understand the molecular mechanisms associated with plant developmental processes. Therefore, we generated HB31SRDX and HB21SRDX repressor mutant lines to understand the functional role of ZF-HD TFs. The mutant lines showed severe defects in plant architecture, including increased branching number, reduced plant height, dis- torted floral phenotype, and short silique. We found that HB31 and HB21 are paralogs in Arabidopsis, and both positively regulate cell size-related genes, cell wall modification factor-related genes, and M-type MADS-box TF families. In addition, HB31 and HB21 are negatively associated with abiotic stress-related genes, vegetative-to-reproductive phase transition of meristem-related genes, and TCP and RAV TFs. microRNA164 (miR164), miR822, miR396, miR2934, and miR172 were downregulated, whereas miR169, miR398, miR399, and miR157 were upregulated in the two repressor lines. Phenotypic and molecular analyses demonstrated that the miR396/GRF modules regulated by HB31 and HB21 are involved in the plan floral architecture of Arabidopsis. The findings of this study will help elucidate the role of ZF-HD TFs in maintaining the floral architecture.
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Kubota, Akane,Ito, Shogo,Shim, Jae Sung,Johnson, Richard S.,Song, Yong Hun,Breton, Ghislain,Goralogia, Greg S.,Kwon, Michael S.,Laboy Cintró,n, Dianne,Koyama, Tomotsugu,Ohme-Takagi, Masaru,Prune Public Library of Science 2017 PLoS genetics Vol.13 No.6
<▼1><P>Photoperiod is one of the most reliable environmental cues for plants to regulate flowering timing. In <I>Arabidopsis thaliana</I>, CONSTANS (CO) transcription factor plays a central role in regulating photoperiodic flowering. In contrast to posttranslational regulation of CO protein, still little was known about <I>CO</I> transcriptional regulation. Here we show that the CINCINNATA (CIN) clade of class II TEOSINTE BRANCHED 1/ CYCLOIDEA/ PROLIFERATING CELL NUCLEAR ANTIGEN FACTOR (TCP) proteins act as <I>CO</I> activators. Our yeast one-hybrid analysis revealed that class II CIN-TCPs, including TCP4, bind to the <I>CO</I> promoter. TCP4 induces <I>CO</I> expression around dusk by directly associating with the <I>CO</I> promoter <I>in vivo</I>. In addition, TCP4 binds to another flowering regulator, GIGANTEA (GI), in the nucleus, and induces <I>CO</I> expression in a <I>GI</I>-dependent manner. The physical association of TCP4 with the <I>CO</I> promoter was reduced in the <I>gi</I> mutant, suggesting that GI may enhance the DNA-binding ability of TCP4. Our tandem affinity purification coupled with mass spectrometry (TAP-MS) analysis identified all class II CIN-TCPs as the components of the <I>in vivo</I> TCP4 complex, and the <I>gi</I> mutant did not alter the composition of the TCP4 complex. Taken together, our results demonstrate a novel function of CIN-TCPs as photoperiodic flowering regulators, which may contribute to coordinating plant development with flowering regulation.</P></▼1><▼2><P><B>Author summary</B></P><P>For plant adaptation to seasonal environments, a crucial developmental event is flowering, as proper timing of flowering affects reproductive success. Although plants monitor various environmental parameters to optimize this timing, photoperiod information is important for plants to regulate seasonal flowering time, because changes in photoperiod occur in a predictable manner throughout the year. The model plant <I>Arabidopsis thaliana</I> responds to photoperiodic changes and flowers under long-day conditions. Based on genetic analyses using mutants defective in the photoperiodic flowering response, we learned that the transcription factor referred to as CONSTANS (CO) plays a central role in regulating the timing of flowering by directly controlling the expression of florigen (flowering-inducing substrate) gene. Long-day afternoon expression of <I>CO</I> is critical for this regulation; however, we had limited knowledge of <I>CO</I> transcriptional regulation. Here we identified that a group of plant-specific transcription factors belonging to the <I>TCP</I> gene family function as novel <I>CO</I> transcriptional activators. We demonstrated that TCP transcription factors regulate <I>CO</I> transcription together with known regulators of <I>CO</I>. Our results imply that plants utilize multiple transcription factors to precisely coordinate the expression of the key regulator gene, <I>CO</I>, which will directly affect flowering time.</P></▼2>
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