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Phenolic Compounds in Antimicrobial Therapy
Santi M. Mandal,Renata O. Dias,Octavio L. Franco 한국식품영양과학회 2017 Journal of medicinal food Vol.20 No.10
Long-term treatment with several conventional antibiotics can cause harmful side effects that can be alleviated by antioxidant therapy. Phenolic compounds (PCs) are the best source of antioxidants, and to identify the most suitable polyphenols for use as a supportive supplement during antibiotic therapy, this study screened a series of PCs to establish their antibacterial potential, including their biofilm and β-lactamase inhibition activity. Several PCs were tested for antibacterial activity against Staphylococcus epidermidis and Pseudomonas aeruginosa. Among them, tannic acid, epigallocatechin gallate, rutin, and eugenol showed the highest antibacterial activity. Epigallocatechin gallate, tannic acid, quercetin, and epicatechin inhibited a significant level of β-lactamase activity. Tannic acid and epigallocatechin gallate presented the highest β-lactamase inhibition potential in both in vitro and in silico analysis. In the present work, these two PCs were the most active agents, presenting both antibacterial activity and β-lactamase and biofilm inhibition ability.
Bonavides, Krishna B.,Pelegrini, Patricia B.,Laumann, Raul A.,Grossi-De-Sa, Maria F.,Bloch, Carlos Jr.,Melo, Jorge A.T.,Quirino, Betania F.,Noronha, Eliane F.,Franco, Octavio L. Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.4
The endophytic bruchid pest Callosobruchus maculatus causes severe damage to storage cowpea seeds, leading to economical losses. For this reason the use of $\alpha$-amylase inhibitors to interfere with the pest digestion process has been an interesting alternative to control bruchids. With this aim, $\alpha$-amylase inhibitors from baru seeds (Dipteryx alata) were isolated by affinity chromatographic procedures, causing enhanced inhibition of C. maculatus and Anthonomus grandis $\alpha$-amylases. To attempt further purification, this fraction was applied onto a reversed-phase HPLC column, generating four peaks with remarkable inhibition toward C. maculatus $\alpha$-amylases. SDS-PAGE and MALDI-ToF analysis identified major proteins of approximately 5.0, 11.0, 20.0 and 55 kDa that showed $\alpha$-amylase inhibition. Results of in vivo bioassays using artificial seeds containing 1.0% (w/w) of baru crude extract revealed 40% cowpea weevil larvae mortality. These results provide evidence that several $\alpha$-amylase inhibitors classes, with biotechnological potential, can be isolated from a single plant species.