Automated Assignments of N- and O-Site Specific Glycosylation with Extensive Glycan Heterogeneity of Glycoprotein Mixtures

Strum, John S.,Nwosu, Charles C.,Hua, Serenus,Kronewitter, Scott R.,Seipert, Richard R.,Bachelor, Robert J.,An, Hyun Joo,Lebrilla, Carlito B. American Chemical Society 2013 ANALYTICAL CHEMISTRY - Vol.85 No.12

<P>Site-specific glycosylation (SSG) of glycoproteins remains a considerable challenge and limits further progress in the areas of proteomics and glycomics. Effective methods require new approaches in sample preparation, detection, and data analysis. While the field has advanced in sample preparation and detection, automated data analysis remains an important goal. A new bioinformatics approach implemented in software called GP Finder automatically distinguishes correct assignments from random matches and complements experimental techniques that are optimal for glycopeptides, including nonspecific proteolysis and high mass resolution liquid chromatography/tandem mass spectrometry (LC/MS/MS). SSG for multiple N- and O-glycosylation sites, including extensive glycan heterogeneity, was annotated for single proteins and protein mixtures with a 5% false-discovery rate, generating hundreds of nonrandom glycopeptide matches and demonstrating the proof-of-concept for a self-consistency scoring algorithm shown to be compliant with the target-decoy approach (TDA). The approach was further applied to a mixture of N-glycoproteins from unprocessed human milk and O-glycoproteins from very-low-density-lipoprotein (vLDL) particles.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2013/ancham.2013.85.issue-12/ac4006556/production/images/medium/ac-2013-006556_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac4006556'>ACS Electronic Supporting Info</A></P>

Yeast SREBP Cleavage Activation Requires the Golgi Dsc E3 Ligase Complex

Stewart, Emerson ,V.,Nwosu, Christine ,C.,Tong, Zongtian,Roguev, Assen,Cummins, Timothy ,D.,Kim, Dong-Uk,Hayles, Jacqueline,Park, Han-Oh,Hoe, Kwang-Lae,Powell, David ,W.,Krogan, Nevan& Elsevier 2011 Molecular cell Vol.42 No.2

<P><B>Summary</B></P><P>Mammalian lipid homeostasis requires proteolytic activation of membrane-bound sterol regulatory element binding protein (SREBP) transcription factors through sequential action of the Golgi Site-1 and Site-2 proteases. Here we report that while SREBP function is conserved in fungi, fission yeast employs a different mechanism for SREBP cleavage. Using genetics and biochemistry, we identified four genes <I>d</I>efective for <I>S</I>REBP <I>c</I>leavage, <I>dsc1-4</I>, encoding components of a transmembrane Golgi E3 ligase complex with structural homology to the Hrd1 E3 ligase complex involved in endoplasmic reticulum-associated degradation. The Dsc complex binds SREBP and cleavage requires components of the ubiquitin-proteasome pathway: the E2-conjugating enzyme Ubc4, the Dsc1 RING E3 ligase, and the proteasome. <I>dsc</I> mutants display conserved aggravating genetic interactions with components of the multivesicular body pathway in fission yeast and budding yeast, which lacks SREBP. Together, these data suggest that the Golgi Dsc E3 ligase complex functions in a post-ER pathway for protein degradation.</P> <P><B>Graphical Abstract</B></P><P><ce:figure id='dfig1'></ce:figure></P><P><B>Highlights</B></P><P>► Yeast SREBP is proteolytically activated by a different mechanism than mammalian SREBP ► Deletion collection screen identified four <I>dsc</I> genes required for fission yeast SREBP cleavage ► Dsc proteins form a Golgi E3 ligase complex that resembles Hrd1 E3 ligase in ERAD ► Yeast SREBP cleavage requires activities of the ubiquitin-proteasome pathway</P>

Effects of Acupressure and Acupuncture-Like Transcutaneous Electrical Nerve Stimulation on Sleep Quality Among Pregnant Women

Ganiyu Sokunbi,Ibrahim U. Takai,Ifeoma B. Nwosu,Rasheedat Balarabe 사단법인약침학회 2020 Journal of Acupuncture & Meridian Studies Vol.13 No.6

Background: Despite the adverse consequences of poor sleep during pregnancy, relatively few safe interventions exist for improving sleep among pregnant women. Nonpharmacological interventions are increasingly gaining acceptance. However, the effects of acupressure and acupuncture-like transcutaneous electrical nerve stimulation (ACUTENS) have not been widely reported. Objectives: The purpose of this study is to investigate the effects of low-frequency transcutaneous electrical nerve stimulation, otherwise known as ACUTENS, and acupressure on sleep quality among pregnant women. Methods: Purposive sampling of 42 pregnant women with sleep disorders was conducted, and the participants were randomized into 3 groups, i.e., acupressure, ACUTENS, and usual care. interventions, which were carried out twice weekly for six weeks. The Pittsburgh Sleep Quality index was used to determine the overall sleep quality. Results: The preintervention scores of sleep quality were 12.5 (3.75), 12.5 (4.0), and 13.0 (3.5) in the ACUTENS, acupressure, and usual care groups, respectively. The KruskaleWallis test did not show any significant difference in the preintervention sleep quality scores (H = 0.379, p = 0.827). Participants in the usual care group recorded the least improvement, with a score of 5.0 (2.25), the ACUTENS group recorded a score of 4.5 (3.0), and the greatest improvement was recorded in the acupressure group, with a score of 4.0 (2.2) after 6 weeks of intervention. The KruskaleWallis test did not show any significant difference in the postintervention sleep quality scores among the 3 groups (H = 0.666, p = 0.717). Conclusion: This study showed that ACUTENS and acupressure as an adjunct to usual care are not more effective than usual care alone in improving sleep quality among pregnant women.

Glyco-Analytical Multispecific Proteolysis (Glyco-AMP): A Simple Method for Detailed and Quantitative Glycoproteomic Characterization

Hua, Serenus,Hu, Chloe Y.,Kim, Bum Jin,Totten, Sarah M.,Oh, Myung Jin,Yun, Nayoung,Nwosu, Charles C.,Yoo, Jong Shin,Lebrilla, Carlito B.,An, Hyun Joo American Chemical Society 2013 Journal of proteome research Vol.12 No.10

<P>Despite recent advances, site-specific profiling of protein glycosylation remains a significant analytical challenge for conventional proteomic methodology. To alleviate the issue, we propose glyco-analytical multispecific proteolysis (Glyco-AMP) as a strategy for glycoproteomic characterization. Glyco-AMP consists of rapid, in-solution digestion of an analyte glycoprotein (or glycoprotein mixture) by a multispecific protease (or protease cocktail). Resulting glycopeptides are chromatographically separated by isomer-specific porous graphitized carbon nano-LC, quantified by high-resolution MS, and structurally elucidated by MS/MS. To demonstrate the consistency and customizability of Glyco-AMP methodology, the glyco-analytical performances of multispecific proteases subtilisin, pronase, and proteinase K were characterized in terms of quantitative accuracy, sensitivity, and digestion kinetics. Glyco-AMP was shown be effective on glycoprotein mixtures as well as glycoproteins with multiple glycosylation sites, providing detailed, quantitative, site- and structure-specific information about protein glycosylation.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jprobs/2013/jprobs.2013.12.issue-10/pr400442y/production/images/medium/pr-2013-00442y_0010.gif'></P>

Knowledge of the negative effect of Cigarette Smoking among adult in sokoto metropolis

John, Ikpeama Osita,Mariam, Onuzulike Nonye,Adimabua, Okafor Patrick,Anthonia, Ikpeama Chizoba,Joy, Ikpeama Chinwe,Osazuwa, Igbineweka Osa,Andrew, Ikpeama Emeka,Jacob, Ofuenyi,Paulastella, Nwosu Nched Korea FoodHealth Convergence Association 2019 식품보건융합연구 (KJFHC) Vol.5 No.2

This study was aimed at ascertaining the level of knowledge of the health effects of cigarette smoking on health and wellbeing among adults. A cross-sectional form of descriptive survey research design was used for this study. Two hundred and seventy copies of the questionnaire collected were analyzed using descriptive statistic of frequency count, normative percentage and grand mean; as well as inferential statistics of chi-square (${\chi}^2$). The level of significant was fixed at 0.05. Appropriate degrees of freedom were worked out. The influence of gender on the knowledge of the negative effect of cigarette smoking among adults in sokoto. The results shows that 48% agreed on the negative effects of showing among male followed by 29.3% among female, those who undecided 8.8% male and 4.4% females while those who disagree are 6.2% for male and 2.5% for female. The research concluded that the knowledge of negative effect of cigarette smoking among adults in sokoto metropolis was very good across the different variable (marital status, gender, and year in higher institution) with single, male and first year in higher institution showing significantly good knowledge of the negative effect of cigarette smoking.

Awareness of Predisposing Factor to Smoking among Adult in Sokoto

Ikpeama Osita John,Onuzulike Nonye Mariam,Okafor Patrick Adimabua,Ikpeama Chizoba Anthonia,Ikpeama Chinwe Joy,Igbineweka Osa Osazuwa,Ikpeama Emeka Andrew,Ofuenyi Jacob,Nwosu Nchedochukwu Paulastella,I 한국식품보건융합학회 2019 식품보건융합연구 (KJFHC) Vol.5 No.1

Smoking has become one of the public health harzard affecting the world. In the UK, smoking is responsible for around one in five deaths. The illnesses caused by smoking extend beyond the well-reported links with cancer, heart disease and respiratory illnesses. Hence the research to determine the awareness of the predisposing factor to smoking among adults in sokoto metropolis. A cross-sectional form of descriptive survey research design was used for this study. This is because descriptive studies are used when the characteristics of a population are either unknown or partially known (Hennekens & Buring, 2007), and it was used by Ganley and Rosario (2013) in a related researchthis justified the use of similar design in a study of similar nature.Two hundred and seventy returned questionnaire was collected, analyzed using descriptive statistic of frequency count, normative percentage and grand mean; as well as inferential statistics of chi-square (χ2). The level of significant was fixed at 0.05. Appropriate degrees of freedom were worked out. There was statistical significant influence or relationship with marital status on the predisposing factors of smoking chi-square of 19716.516 greater than the critical value 43.77297at df 30 p<0.05. There were statistical significance chi-square =27468.348 which is greater than the critical value 43.77297 at df= 30. These show that there is a relationship on gender awareness of predisposing factors to smoking rejecting the null hypotheses. The respondents across different lever/year higher institution shows that the awareness of predisposing factors of smoking there were a statistical significance difference chi-square =7168.429 (df=88) greater than critical value 102.342 rejecting the null hypotheses. There is consistent evidence that links exposure to depictions of smoking in movies and initiation of smoking in young people. Over the years television shows and films have effectively built up associations between smoking and g

Knowledge of the negative effect of Cigarette Smoking among adult in sokoto metropolis

Ikpeama Osita John,Onuzulike Nonye Mariam,Okafor Patrick Adimabua,Ikpeama Chizoba Anthonia,Ikpeama Chinwe Joy,Igbineweka Osa Osazuwa,Ikpeama Emeka Andrew,Ofuenyi Jacob,Nwosu Nchedochukwu Paulastella,I 한국식품보건융합학회 2019 식품보건융합연구 (KJFHC) Vol.5 No.2

This study was aimed at ascertaining the level of knowledge of the health effects of cigarette smoking on health and wellbeing among adults. A cross-sectional form of descriptive survey research design was used for this study. Two hundred and seventy copies of the questionnaire collected were analyzed using descriptive statistic of frequency count, normative percentage and grand mean; as well as inferential statistics of chi-square (χ2). The level of significant was fixed at 0.05. Appropriate degrees of freedom were worked out. The influence of gender on the knowledge of the negative effect of cigarette smoking among adults in sokoto. The results shows that 48% agreed on the negative effects of showing among male followed by 29.3% among female, those who undecided 8.8% male and 4.4% females while those who disagree are 6.2% for male and 2.5% for female. The research concluded that the knowledge of negative effect of cigarette smoking among adults in sokoto metropolis was very good across the different variable (marital status, gender, and year in higher institution) with single, male and first year in higher institution showing significantly good knowledge of the negative effect of cigarette smoking.