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Nemeno, Judee Grace E.,Lee, Soojung,Yang, Wojong,Lee, Kyung Mi,Lee, Jeong Ik Hindawi Publishing Corporation 2014 BioMed research international Vol.2014 No.-
<P>Drug repositioning is one of the most rapidly emerging fields of study. This concept is anchored on the principle that diseases have similar damaged or affected signaling pathways. Recently, drugs have been repositioned not only for their alternative therapeutic uses but also for their applications as biomaterials in various fields. However, medical drugs as biomaterials are rarely focused on in reviews. Fragmin and protamine have been recently the sources of increasing attention in the field of tissue engineering and regenerative medicine. Fragmin and protamine have been manufactured primarily as a safe antidote for the circulating heparin. Lately, these drugs have been utilized as either micro- or nanoparticle biomaterials. In this paper, we will briefly describe the concept of drug repositioning and some of the medical drugs that have been repurposed for their alternative therapeutic uses. Also, this will feature the historical background of the studies focused on fragmin/protamine micro/nanoparticles (F/P M/NPs) and their applications as biomaterials in tissue engineering, stem cell therapy, and regenerative medicine.</P>
Balolong, Ernesto,Lee, Soojung,Nemeno, Judee Grace,Lee, Jeong Ik Hindawi Publishing Corporation 2016 Stem cells international Vol.2016 No.-
<P>There is an increasing concern that the term adipose tissue-derived stem cell (ASC) is inappropriately used to refer to the adipose stromal vascular fraction (SVF). To evaluate the accuracy and quality of reporting, 116 manuscripts on the application of ASC in humans and animals were examined based on the 2013 published International Federation for Adipose Therapeutics and Science (IFATS)/ International Society for Cellular Therapy (ISCT) joint statement and in reference to current guidelines for clinical trials and preclinical studies. It is disconcerting that 4 among the 47 papers or 8.51% (CI 2.37–20.38) surveyed after publication of IFATS/ISCT statement reported using ASCs but in fact they used unexpanded cells. 28/47 or 59.57% (CI 44.27–73.63) explicitly reported that adherent cells were used, 35/47 or 74.47% (CI 59.65–86.06) identified expression of surface markers, and 25/47 or 53.19% (CI 14.72–30.65) verified the multilineage potential of the cells. While there are a number of papers examined in this survey that were not able to provide adequate information on the characteristics of ASCs used with some erroneously referring to the SVF as stem cells, there are more room for improvement in the quality of reporting in the application of ASCs in humans and animals.</P>
박승화,윤지원,박재현,김보영,Judee Grace E. Nemeno,Ernesto C. Balolong Jr,한유나,이경미,이수정,이정익 한국조직공학과 재생의학회 2015 조직공학과 재생의학 Vol.12 No.4
This article overall describes the development of histological method especially, cartilage and bone research in regenerative medicine. Kawamoto’s film method, which has been recently introduced into our laboratory, has been applied in various fields that make sectioning of hard tissues easier than the conventional method. Moreover, this method also does not require the time-consuming chemical fixation and/or decalcification process. Kawamoto method involves the use of the adhesive plastic film instead of a cover glass where the thin tissue sections are attached efficiently at low temperatures (-25°C). Furthermore, the histological method preserves the enzymatic activity in the fresh sections in comparison to that of chemically-treated tissue sections. In fact, we used this Kawamoto’s film method in one of our researches in which drug repositioning was employed for cartilage regeneration. Some of the Kawamoto-processed tissue sections are featured in this review article. Therefore, the application of this tissue preparation technique allowed effective and histological and histochemical studies within a shorter preparation time with ease and convenience. In the future, this Kawamoto method may offer further applications in the preparation of a more diverse tissues and samples not only in the preclinical but also in the clinical studies.