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A novel HSF1-mediated death pathway that is suppressed by heat shock proteins
Hayashida, Naoki,Inouye, Sachiye,Fujimoto, Mitsuaki,Tanaka, Yasunori,Izu, Hanae,Takaki, Eiichi,Ichikawa, Hitoshi,Rho, Jaerang,Nakai, Akira Wiley (John WileySons) 2006 The EMBO journal Vol.25 No.20
<P>Heat shock response is an adoptive response to proteotoxic stress, and a major heat shock transcription factor 1 (HSF1) has been believed to protect cells from cell death by inducing heat shock proteins (Hsps) that assist protein folding and prevent protein denaturation. However, it is revealed recently that HSF1 also promotes cell death of male germ cells. Here, we found a proapoptotic Tdag51 (T-cell death associated gene 51) gene as a direct target gene of HSF1. Heat shock and other stresses induced different levels of Hsps and Tdag51, which depend on cell types. Hsps bound directly to the N-terminal pleckstrin-homology like (PHL) domain of Tdag51, and suppressed death activity of the C-terminal proline/glutamine/histidine-rich domain. Tdag51, but not major Hsps, were induced in male germ cells exposed to high temperatures. Analysis of Tdag51-null testes showed that Tdag51 played substantial roles in promoting heat shock-induced cell death in vivo. These data suggest that cell fate on proteotoxic condition is determined at least by balance between Hsp and Tdag51 levels, which are differently regulated by HSF1.</P>
Masahiko Kobayashi,Jian Xu,Kohei Kakino,Akitsu Masuda,Masato Hino,Naoki Fujimoto,Kosuke Minamihata,Noriho Kamiya,Hiroaki Mon,Hiroshi Iida,Masateru Takahashi,Takahiro Kusakabe,이재만 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.1
Interleukine-4 (IL-4) is a cytokine that plays an important role in the immune system and recognized as a biological medicine. Therefore, there is a demand for the production of IL-4 with high performance. The expression of a recombinant IL-4 protein in the prokaryotic system usually results in the formation of an inclusion body. To date, the solution to obtain those active products without the refolding process remains to be established. In this study, we tried to acquire a biologically active recombinant Mus musculus IL-4 (rMmIL-4) using a silkworm-baculovirus expression vector system (silkworm-BEVS). We constructed two recombinant baculoviruses coding rMmIL-4 with the distinct location of affinity purification tags and succeeded in the expression and purification of rMmIL-4 proteins directly without the refolding process. Both purified proteins displayed comparable biological activity to the commercial proteins produced by the E. coli expression system. Besides, we performed screening of silkworm strains to seek optimal hosts for the mass-production of rMmIL-4. Intriguingly, we found that some silkworm strains showed significantly higher secretion levels of rMmIL-4 in silkworm sera. Our study provides meaningful insights into the industrial-scale production of rMmIL-4 with high productivity for pharmaceutical applications in the future.
Study of a Spherical Ultrasonic Motor for Haptic Display System
Ryota Ishibashi,Tomohiro Ohara,Naoyuki Takesue,Jumpei Arata,Shigeki Toyama,Masahiko Hoshina,Yoshiyuki Hirai,Naoki Fukaya,Hideo Fujimoto 제어로봇시스템학회 2010 제어로봇시스템학회 국제학술대회 논문집 Vol.2010 No.10
In this paper, we examine a Spherical Ultrasonic Motor(SUSM) for the haptic display system. Experimental system consists of a force sensor and a SUSM comprising one spherical rotor and three ring-shaped stators. The stator is made from an elastic body and piezoelectric elements for vibration. The stators and the rotor are in pressure contact with each other. The frequency and the phase difference the stators" vibration can be controlled and we can generate torque of the rotor. Some experiments were briefly investigated to examine the performances of the proposed haptic display method.