Effectiveness of sequence-related amplified polymorphism (SRAP) markers to assess the geographical origin and genetic diversity of collected lentil genotypes

Mohammed Nabil A.,Afzal Muhammad,Al-Faifi Sulieman A.,Khan Muhammad A.,Refay Yahya A.,AL-Samin Bazel H.,Alghamdi Salem S.,Ibrahim Abdullah 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.4

Lentil is an important annual food legume crop, nitrogen fixer and provides a substantial amount of protein, carbohydrate, minerals, and vitamin content. The use of molecular markers to assess genetic diversity is crucial for crop improvement, efficient management, and conservation of plant genetic resources. The current study aimed to determine the genetic diversity among lentil genotypes using sequence-related amplified polymorphism (SRAP) markers. Therefore, we evaluated a collection of 36 lentil genotypes, including 20 from Yemen, Saudi Arabia (7), Egypt (4), and Bangladesh (3), and (2) genotypes from the International Center for Research in Dry Area (ICARDA) using 21 SRAP primer combinations. The amplified fragments showed a high level of useful polymorphic amplified fragments (775 out of 782) indicating a higher degree of variation. The polymorphic information content (PIC) ranged from 0.31 to 0.39 with an average of 0.33 for each primer. The UPGMA trees, based on Jaccard similarity index matrices, separated the genotypes into four main clusters according to their geographical origin. The population structure supported the major groups and attested to their great degree of differentiation. The highest lentil population was found at K = 3, K = 5, and K = 7 levels, showing purity and admixture ancestry among the lentil population. This study highlighted the wide genetic diversity among the studied lentil genotypes and demonstrated the effectiveness of the SRAP technique in determining the genetic variability of lentil. Furthermore, it could be used to establish the genetic peculiarity of ecotypes when applying for the obtainment of origin and agro-morphological characteristics.

Application of MCT-βCD to Modify Cellulose/Wool Blended Fabrics for Upgrading Their Reactive Printability and Antibacterial Functionality

Nabil A. Ibrahim,Heba M. Khalil,Basma M. Eid,Tawfik M. Tawfik 한국섬유공학회 2018 Fibers and polymers Vol.19 No.8

Pre-loading of monochlorotriazinyl β-Cyclodextrin (MCT-βCD) onto/within viscose/wool (V/W) and cotton/wool (C/W) blended fabrics provide hosting cavities that can form host-guest inclusion complexes with reactive dyes in postprinting as well as with triclosan derivative or silver nanoparticles/hyperbranched polyamide-amine (AgNPs/HBPAA) composite in subsequent final antibacterial finishing step. Coloration properties, antibacterial activity against (S. aureus) and (E. coli) pathogenic bacteria, durability of the obtained products, according to the above mentioned route, to wash, surface morphology and composition of selected samples were investigated. Results obtained signify that premodification of the nominated substrates with MCT- βCD (10 g/l), followed by reactive printing with mono-or bifunctional reactive dye (20 g/l), and subsequent post-finishing with triclosan derivative or AgNPs/HBPAA composite (15 g/l each) is an efficient treatments sequence for attaining reactive prints with significant antibacterial efficacy and noticeable durability to wash. Surface depositions of selected active ingredients were also confirmed using SEM and EDX analysis.

Environmentally benign Scouring of Cotton Knits Using Locally Produced Acid Pectinase Enzyme

Nabil A. Ibrahim,Basma M. Eid,Mohamed S. Abdel Aziz,Soha M. Hamdy,Safaa E. AbdAllah 한국섬유공학회 2019 Fibers and polymers Vol.20 No.4

The main task of the present study is to pre-screen selected fungal strain (Fusarium oxysporum M1-EGY) isolatedfrom local agriculture soil and optimize cultivation conditions for an acid pectinase production. Experimental resultsdemonstrated the optimum conditions for achieving the highest enzyme activity (14.80 U/ml) were found to be: incubationperiod (8 days) at pH (5), temperature (30 oC), and shaking rate (150 rpm), apple pectin carbon source (2 %), and ammoniumphosphate as nitrogen source (0.2 gN/l). The positive impacts of utilizing the produced crude acid-pectinase in eco-friendlyscouring of various cotton knits as well as their subsequent bleaching and reactive dyeing have been confirmed. Additionally,the use of local resources for an acid pectinase production would encourage and promote local production of textile enzymesand their potential applications in Egyptian textile sector, taking in consideration efficiency, textile quality, environmental andeconomy concerns.

A Greener Facile Approach to Develop Durable Multifunctional Cellulosic Pigment Prints Using b-CD, ZnO-NPs and Several Bioactive Guest Molecules

Nabil A. Ibrahim,Enas M. El‑Zairy,Shimaa S. Abd Allah,El‑Amir M. Emam 한국섬유공학회 2023 Fibers and polymers Vol.24 No.1

In the present work, β-CD, ZnO-NPs, and several eco-friendly bioactive guests, namely, cinnamon oil, lemon oil, lavenderoil, and vanillin along with environmentally benign pigment paste constituents were applied to cotton, viscose, cotton/viscose, and cotton/linen cellulosic substrates to develop durable multi-functional pigment prints in a single step using thepad-dry-thermofixation method. The developed one-step pigment coloration and multi-functionalization method resulted ina remarkable improvement in antibacterial efficacy, UV-protection ability, aroma property along with a noticeable increasein depth of the obtained pigment prints, making them very useful for a wide range of potential applications. The extent ofenhancement in the above-mentioned functional and coloration properties is governed by type of cellulosic substrate, presenceof β-CD/ZnO-NPs, type of bioactive agent as well as kind of pigment colorant. Among the used bioactive agents, lavenderoil proved to be the best for attaining multi-functional and pigment coloration properties, keeping other parameters constant. The obtained results also demonstrated that increasing washing cycles up to 15 washes resulted in a slight decrease in theimparted functional and coloration properties, regardless of the used cellulosic substrate. Both SEM images and EDX spectraconfirmed the surface modification and ZnO-NPs immobilization onto the selected fabric surfaces. Mode of interactionsamong the different printing paste constituents and the cellulosic substrates were also suggested.

An Eco-Friendly Multifunctional Nano-Finishing of Cellulose/Wool Blends

Nabil A. Ibrahim,El-Amir M. Emam,Basma M. Eid,Tawfik M. Tawfik 한국섬유공학회 2018 Fibers and polymers Vol.19 No.4

A new approach for an eco-friendly multi-functionalization of cotton/wool (C/W) and viscose/wool (V/W) blended fabrics was investigated. In this study, Ag-nanoparticle (Ag-NP) and/or ZnO-nanoparticle (ZnO-NP) functional agents were incorporated into the finishing bath along with citric acid (CA) or succinic acid (SA) as ester-crosslinking or esterifying agent, and sodium hypophosphite catalyst using the padding technique. The obtained results indicated that the extent of multi-functionalization expressed as antibacterial activity, UV-blocking functionality and wrinkle recovery ability were determined by kind of nanomaterial, nature of carboxylic acid, i.e., bi- or tri-functional and type of substrate. The results also demonstrated that blended fabrics finished with Ag-NP/ZnO-NP/CA/SHP nano-finishing formulation exhibited outstanding durable multi-functional properties even after 10 washing cycles. In addition, the change in surface morphology and the existence of Ag and/or Zn onto the selected V/W fabric surfaces have been confirmed by SEM and EDX analysis respectively.

Multipurpose Treatment of Cellulose-Containing Fabrics to Impart Durable Antibacterial and Repellent Properties

Nabil A. Ibrahim,Ahmed Amr,Basma M. Eid 한국섬유공학회 2020 Fibers and polymers Vol.21 No.3

Development of multifunctional cellulose-containing fabrics has been highly demanded both by the textile finisherand the textile consumer. Herein, proper finishing formulations comprise the following ingredients individually and incombination: a reactant crosslinker (Fixapret® ECO), hydrolysed 3-glycidyl-oxypropyltrimethoxysilane (GPTMS), as a cocrosslinkerand fixing agent, a flurochemical water/oil repellent agent (Asahi Guard® AG-925), silver nanoparticlesdispersion (AgNPs), as antibacterial agent along with metal salt catalyst, especially AlCl3, were applied to various cellulosecontaining fabrics, using a pad-coating impregnation method, to add new multifunctional properties like anti-crease, water/oilrepellent, antibacterial functionality as well as thermal stability. The degree of improvement in the imparted functions isgoverned by type of substrate, extent of modification and/or crosslinking, amount/location/distribution as well as fixation ofthe added active ingredients. Also, the obtained results clearly demonstrated that the created multifunctional properties arenot seriously decreased even after 15 washing cycles. Various characterization techniques were used, and the possibleinteractions among the nominated components and the treated substrates were also suggested.

Green Approach for Multifunctionalization of Cellulose-Containing Fabrics

Nabil A. Ibrahim,Amal A. Aly,Basma M. Eid,Hesham M. Fahmy 한국섬유공학회 2018 Fibers and polymers Vol.19 No.11

Development of multifunctional textile and clothing products with improved environmental profiles has been demanded both by textile industry and by consumer. Herein, dialdehyde sodium alginate (DASA) and dialdehyde carboxymethyl cellulose (DACMC) have been prepared, characterized and utilized, as an eco-friendly binding/ macromolecular crosslinking/hand building agents, in functional finishing of cellulose-containing fabrics. Fabric samples were treated with the nominated dialdehyde polysaccharide (DAPS, 10 g/l) along with the reactant resin (DMDHEU, 50 g/l), Ag- or TiO2-NPs as active ingredients (20 g/l) and ammonium persulfate catalyst (5 g/l) using the padding method. After functional finishing, the finished fabrics demonstrated a remarkable improvement in their antibacterial efficacy, UV-blocking ability, self-cleaning capacity, and surface roughness functionality without adversely affecting fabrics resiliency. The variation in these functional properties is affected by kind of DAPS, type of added nanoparticles as well as the treated substrate. Additionally, FTIR, SEM, EDS, and durability to wash measurements for selected samples were performed. Moreover, pre-oxidation of DAPS, functionalization reactions/interactions among the nominated reactants and the textile materials were also suggested.

The effect of ultrasound-guided bilateral thoracic retrolaminar block on analgesia after pediatric open cardiac surgery: a randomized controlled double-blind study

Abdelbaser Ibrahim,Mageed Nabil A.,Elfayoumy Sherif I.,Magdy Mohamed,Elmorsy Mohamed M.,ALseoudy Mahmoud M. 대한마취통증의학회 2022 Korean Journal of Anesthesiology Vol.75 No.3

Background: The thoracic retrolaminar block (TRLB) is a relatively new regional analgesia technique that can be used as an alternative to the thoracic paravertebral block. This study aimed to evaluate the postoperative analgesia effects of ultrasound-guided TRLB in children undergoing open cardiac surgery via median sternotomy incision.Methods: Sixty-six patients aged 2–8 years were recruited. In the TRLB group, 0.25% bupivacaine 0.4 ml/kg was injected into the retrolaminar space on both sides at the level of the T4 lamina. Patients in the control group were injected with 0.9% saline. The primary outcome measure was fentanyl consumption in the first 24 h post-extubation. The secondary outcome measures were the total intraoperative fentanyl consumption, postoperative modified objective pain score (MOPS), and time to extubation.Results: The total intraoperative fentanyl requirements and fentanyl consumption in the first 24 h post-extubation were significantly lower (P < 0.001) in the TRLB group (9.3 ± 1.2; 6.9 ± 2.1 μg/kg, respectively) than in the control group (12.5 ± 1.4; 16.6 ± 2.8, respectively). The median (Q1, Q3) time to extubation was significantly shorter (P < 0.001) in the TRLB group (2 [1, 3] h) than in the control group (6 [4.5, 6] h). The MOPS was significantly lower (P < 0.05) in the TRLB group than in the control group at 0, 2, 4, 8, 12 and 16 h post-extubation.Conclusions: Bilateral ultrasound-guided TRLB is effective in providing postoperative analgesia in children undergoing open cardiac surgery via median sternotomy incision.

Preemptive analgesic efficacy of ultrasound-guided transversalis fascia plane block in children undergoing inguinal herniorrhaphy: a randomized, double-blind, controlled study

Abdelbaser Ibrahim,Mageed Nabil A.,El-Emam El-Sayed M.,ALseoudy Mahmoud M.,Elmorsy Mohamed M. 대한마취통증의학회 2021 Korean Journal of Anesthesiology Vol.74 No.4

Background: Surgical repair of congenital inguinal hernia results in significant postoperative discomfort and pain. The aim of the current study was to evaluate the pre-emptive analgesic efficacy of a transversalis fascia plane (TFP) block after pediatric inguinal herniorrhaphy.Methods: Forty-four patients aged 12 to 60 months who underwent unilateral inguinal herniorrhaphy were enrolled. Four patients were excluded, and the remaining were allocated to the control group and the TFP block group. In the TFP block group, 0.4 mL/kg bupivacaine 0.25% was instilled in the plane between the transversus abdominis and transversalis fascia, while in the control group 0.9% saline was used instead of bupivacaine. The collected data were the total dose of paracetamol consumed during the first 12 h postoperatively, the postoperative Face, Leg, Activity, Cry, Consolability (FLACC) pain score, time to first use of rescue analgesia, number of patients required additional postoperative analgesics, and parents' satisfaction.Results: The median paracetamol consumption was significantly lower in the TFP block group than in the control group, and FLACC pain scores were significantly lower for all study times in the TFP block group with higher parental satisfaction scores than those for the control group. The number of patients who required additional analgesics was significantly lower in the TFP block group than in the control group. Conclusions: The use of a TFP block decreases postoperative analgesic consumption and postoperative pain intensity after pediatric inguinal herniorrhaphy. Future studies with larger sample size are required to evaluate the actual complications rate of TFP block.

HYDROMAGNETIC ROTATING DISK FLOW OF A NON-NEWTONIAN FLUID WITH HEAT TRANSFER AND OHMIC HEATING

Hazem A. Attia,Karem M. Ewis,Ibrahim H.Abd Elmaksoud,Nabil A.Awad-Allah 한국산업응용수학회 2012 Journal of the Korean Society for Industrial and A Vol.16 No.3

The steady hydromagnetic flow of an electrically conducting non-Newtonian fluid due to the rotation of an infinite disk is studied with heat transfer with the inclusion of the ion slip as well as Ohmic heating. The governing nonlinear momentum equations and energy equations are solved using the finite difference method. The numerical results indicate the important effect of the ion slip and the non-Newtonian fluid characteristics on the velocity and temperature distributions.