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RISS 인기검색어
- 검색키워드
- 저자 : N.C. Jayasinghe (검색결과 3 건)
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Bathige, S.D.N.K.,Umasuthan, N.,Jayasinghe, J.D.H.E.,Godahewa, G.I.,Park, H.C.,Lee, J. Academic Press 2016 FISH AND SHELLFISH IMMUNOLOGY Vol.56 No.-
<P>The globular C1q (gC1q) domain containing proteins, commonly referred as C1q domain containing (C1qDC) proteins, are an essential family of proteins involved in various innate immune responses. In this study, three novel C1qDC proteins were identified from the disk abalone (Haliotis discus discus) transcriptome database and designated as AbC1qDC1, AbC1qDC2, and AbC1qDC3. The cDNA sequences of AbC1qDC1, AbC1qDC2, and AbC1qD0 consisted of 807, 1305, and 660 bp open reading frames (ORFs) encoding 269, 435, and 220 amino acids (aa), respectively. Putative signal peptides and the N-terminal gC1q domain were identified in all three AbC1qDC proteins. An additional predicted motif region, known as the coiled coil region (CCR), was identified next to the signal sequence of AbC1qDC2. The genomic organization of the AbC1qDCs was determined using a bacterial artificial chromosome (BAC) library. It was found that the CDS of AbC1qDC1 was distributed among three exons, while the CDSs of AbC1qDC2 and AbC1qDC3 were distributed between two exons. Sequence analysis indicated that the AbC1qDC proteins shared <40% identity with other counterparts from different species. According to the neighbor joining phylogenetic tree, the proteins were grouped within an invertebrate group with high evolutionary distances, which suggests that they are new members of the C1qDC family. Higher expression of AbC1qDC1 and AbC1qDC2 was detected in hepatopancreas, muscle, and mantle tissues compare to the other tissues analyzed, using reverse transcription, followed by quantitative real-time PCR (qPCR) using SYBR Green, whereas AbC1qDC3 was predominantly expressed in gill tissues, followed by muscles and the hepatopancreas. The temporal expression of AbC1qDC transcripts in gills after bacterial (Vibrio parahaemolyticus and Listeria monocytogenes) and lipopolysaccharide stimulation indicated that AbC1qDCs can be strongly induced by both Gram-negative and Gram-positive bacterial species with different response profiles. The results of this study suggest that AbC1qDCs are involved in immune responses against invading bacterial pathogens. (C) 2016 Published by Elsevier Ltd.</P>
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Vulnerability of roofing components to wind loads
N.C. Jayasinghe,J.D. Ginger 한국풍공학회 2011 Wind and Structures, An International Journal (WAS Vol.14 No.4
The vulnerability of roofing components of contemporary houses built in cyclonic regions of Australia is assessed for increasing wind speeds. The wind loads and the component strengths are treated as random variables with their probability distributions derived from available data, testing, structural analysis and experience. Design details including types of structural components of houses are obtained from surveying houses and analyzing engineering drawings. Wind load statistics on different areas of the roof are obtained by wind tunnel model studies and compared with Australian/New Zealand Standard, AS/NZS 1170.2. Reliability methods are used for calculating the vulnerability of roofing components independently over the roof. Cladding and batten fixings near the windward gable edge are found to experience larger negative pressures than prescribed in AS/NZS 1170.2, and are most vulnerable to failure.
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Vulnerability of roofing components to wind loads
Jayasinghe, N.C.,Ginger, J.D. Techno-Press 2011 Wind and Structures, An International Journal (WAS Vol.14 No.4
The vulnerability of roofing components of contemporary houses built in cyclonic regions of Australia is assessed for increasing wind speeds. The wind loads and the component strengths are treated as random variables with their probability distributions derived from available data, testing, structural analysis and experience. Design details including types of structural components of houses are obtained from surveying houses and analyzing engineering drawings. Wind load statistics on different areas of the roof are obtained by wind tunnel model studies and compared with Australian/New Zealand Standard, AS/NZS 1170.2. Reliability methods are used for calculating the vulnerability of roofing components independently over the roof. Cladding and batten fixings near the windward gable edge are found to experience larger negative pressures than prescribed in AS/NZS 1170.2, and are most vulnerable to failure.
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