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Muniyandi Jeyaraj,Sangiliyandi Gurunathan,Senthilkumar Varadan,Kevin John Pulikotil Anthony,Muralidharan Murugan,Ashok Raja 한국공업화학회 2013 Journal of Industrial and Engineering Chemistry Vol.19 No.4
Here, we describe biosynthesis of silver nanoparticles by reduction of aqueous Ag+ ions with the culture supernatant of Pseudomonas aeruginosa. The morphological, physiological, biochemical and molecular level identification of the strain GS1 resembles P. aeruginosa. The nanoparticles synthesized by P. aeruginosa were characterized by UV–vis spectroscopy, dynamic light scattering (DLS) and scanning electron microscopy (SEM). The size-distribution of nanoparticles was determined using a particle-size analyzer and the average particle-size was found to be 80 nm. The biological activities of the synthesized silver nanoparticles like antimicrobial activity were confirmed against Escherichia coli and Staphylococcus aureus and it have stable anti-coagulant effect.
( Kalibulla Syed Ibrahim ),( Jeyaraj Muniyandi ),( Shunmugiah Karutha Pandian ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.1
The purification and characterization of a Mn2+-dependent alkaline serine protease produced by Bacillus pumilus TMS55 were investigated. The enzyme was purified in three steps: concentrating the crude enzyme using ammonium sulfate precipitation, followed by gel filtration and cation-exchange chromatography. The purified protease had a molecular mass of approximately 35 kDa, was highly active over a broad pH range of 7.0 to 12.0, and remained stable over a pH range of 7.5 to 11.5. The optimum temperature for the enzyme activity was found to be 60oC. PMSF and AEBSF (1 mM) significantly inhibited the protease activity, indicating that the protease is a serine protease. Mn2+ ions enhanced the activity and stability of the enzyme. In addition, the purified protease remained stable with oxidants (H2O2, 2%) and organic solvents (25%), such as benzene, hexane, and toluene. Therefore, these characteristics of the protease and its dehairing ability indicate its potential for a wide range of commercial applications.
Kevin John Pulikotil Anthony,Muralidharan Murugan,Muniyandi Jeyaraj,Navanietha Krishnaraj Rathinam,Gurunathan Sangiliyandi 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.4
Here we described, cost effective, green and environmentally friendly approach for synthesis of AgNPs using edible pine mushroom Tricholoma matsutake. The synthesized AgNPs were characterized using UV–visible spectroscopy, particle analyzer and X-ray diffraction. Furthermore, Fourier transform infrared spectroscopy (FTIR) measurements were carried out to identify the possible biomolecules responsible for capping and efficient stabilization of the nanoparticles. The size and morphology of nanoparticles were analyzed by transmission electron microscopy (TEM). In addition, the antimicrobial activity of as prepared AgNPs was investigated against Escherichia coli and Bacillus subtilis using the agar diffusion method.
Muralidharan Murugan,Sangiliyandi Gurunathan,Kevin John Pulikotil Anthony,Muniyandi Jeyaraj,Navanietha Krishnaraj Rathinam 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.4
In this paper, a simple and environmentally friendly method for synthesis of gold nanoparticles (AuNPs) using culture supernatant of Bacillus flexus as reductants and stabilizers is reported. The prepared AuNPs were characterized by various analytical techniques. UV–visible spectroscopy, X-ray diffraction (XRD), energy dispersive X-ray spectroscopy (EDS) results confirmed that Au3+ ions reduced into Au0. Fourier-transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) measurements confirmed that the chemical surface structure of AuNPs. TEM images showed the size and morphology of AuNPs. Moreover, the toxicity studies suggested that AuNPs were neither toxic nor inhibitory to human breast cancer cells (MCF-7).