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      • Proteomic Analysis of Barnyard Grass (Echinochloa crusgalli) Subjected to Herbicides Combined with Wood and Rice Vinegar : 제초제와 목초액 및 왕초액 혼용처리 에 따른 피(Echinochloa crusgalli)의 단백체 분석

        Moreno Cueto, Maria Florida 경북대학교 대학원 2012 국내석사

        RANK : 247359

        Two-dimensional gel electrophoresis coupled to mass spectrometry analysis was used to examine the effect of four herbicides namely: Bentazone + Cyhalofop- butyl (BCB) and Butachlor + Clomazone (BUC), Halosulfuron-methyl + Mefenacet + Thiobencarb (HMMT) and Thiobencarb alone (THB), wood vinegar and rice hull vinegar on a weed species barnyard grass (Echinochloa crusgalli) at the proteome level. Treatments included recommended rate (RR: 100%), half recommended rate (HRR: 50%), HRR combined with different wood vinegar (WV) and rice vinegar (RV) concentrations: 1 ml WV/RV per 100, 250 and 500 ml of water and Control or Untreated. Sampling for proteomic analysis was done 3 days and 7 days after the treatment (DAT) of liquid herbicides and solid herbicides, respectively. To support the study’s results parameters height and weight (fresh and dry) were also measured. Examination of 2-D maps derived from barnyard grass leaf tissues revealed the presence of 62 spots displaying a differential expression pattern, out of which 32 were identified. Observed pH ranges from 4-7.5. In detail, proteins which were observed in all the treatments include 60 kDa jasmonate protein and Chaperonin CPN60-2, mitochondrial, which play important roles in plant defense and in the facilitation of the correct folding of imported proteins respectively. The down regulation of four proteins (spot numbers 9, 10, 13 and 14) was observed in almost all of the herbicide treated plant samples. The shortest plant height was obtained by the grass treated with BCB 50 + 100 WV mix; 5.0 cm followed by BUC 100 in the case of liquid herbicides and highest plant height was observed in the untreated grass, as expected. In the case of solid herbicides, HMMT 50 + 250 WV mix was lowest at 6.50. Lowest fresh weight was observed in BCB 100 at 0.062g. In the case of solid herbicides it was lowest at HMMT 50 + 250 RV mix: 0.201g. In terms of dry weight, lowest value was detected in BCB 50 + 100 RV mix: 0.022g, for liquid herbicides treated. As with solid herbicides, lowest dry weight was attained by HMMT 50 + 250 RV mix. Protein content showed great variations on treatment effects. Generally, samples treated with BUC showed lower protein contents, and it was lowest in BUC 50% + 100 WV mix, With regard to solid herbicides, HMMT 50%+ 100 RV mix showed the lowest amount. Overall, the treatments BCB 50 + 100 WV mix HMMT 50 + 250 RV were most effective in controlling barnyard grass across all parameters analyzed. 본 실험은 제초제와 목초액 및 왕초액 혼용처리에 따른 피의 변화 양상을 proteome level에서 알아보고자 실시하였다. 사용?? 제초제는 경엽처리제 Bentazone + Cyhalofop- butyl (BCB)와 Butachlor + Clomazone (BUC), 그리고 토양처리제 Halosulfuron-methyl + Mefenacet + Thiobencarb (HMMT)와 Thiobencarb alone (THB)를 사용하였으며, 처리방법은 기준시용 100%, 기준시용 50%, 기준시용 50%와 목초액 (wood vinegar, WV) 및 왕초액 (rice vinegar, RV)을 100, 250, 500배액을 혼합하여 무처리와 비교하였다. 시료는 경엽처리제는 처리 후 3일, 토양처리제는 처리 후7일에 각각 채취하여2-DE 이미지 ?씔?에 이용하였으며, 또?? 피의 초장과 생체중, 건물중, 단백질 함량을 조사하였다. pH 4-7.5 2-DE 이미지에서 62개의 spot 중 32개를 동정하였다. 모든 처리구에 60 kDa jasmonate protein과 Chaperonin CPN60-2, mitochondrial이 검출되었는데, 이들은 각각 plant defense와 correct protein folding에 관여??다. 그리고 대부?씬? 제초제 처리구에서 spot number 9, 10, 13, 14이 검출되었다. 초장은 경엽처리제의 경우 BCB50 + 100WV 처리구에서 5.0 cm, 토양처리제의 경우는 HMMT 50% + 250 WV 처리구에서 6.50 cm로 가장 짧은 것으로 나타났다. 생체중에서는 BCB 100%와 HMMT 50% + 250 RV 처리구에서 가장 낮게 나타났다. 건물중 조사에서는 경엽처리 시에는 BCB 50% + 250 RV 처리구에서 토양처리 시에는 HMMT 50% + 250 RV 처리구에서 가장 낮게 나타났다. 단백질 함량은 일반적으로 BUC 처리구에서 낮은 단백질 함량을 보였고, BUC 50% + 100 WV 처리구가 가장 낮게 나타났다. 토양처리제에서는 HMMT 50% + 100 RV 처리구가 가장 낮게 나타났다. 이런 결과로 볼 때, 경엽처리제는 BCB 50% + 100 WV 처리구가, 토양처리제는 HMMT 50% + 250WV 처리구가 피를 방제하는데 가장 효과적이었다.

      • MAXIMIZING RENEWABLE ENERGY PENETRATION IN THE GALAPAGOS ISLANDS BY USING ENERGY STORAGE SYSTEMS

        MORENO DIAZ EDGAR ADRIAN 아주대학교 대학원 2019 국내석사

        RANK : 247359

        Renewable Energy has increased its participation in the last decades, and nowadays solar, wind, geothermal and waste energy represents around 7% of total electricity production worldwide [1]. Depending on the renewable energy source, its integration, and especially operation, it requires detailed technical and economic analyses. Due to its topology, electrical power infrastructure in islands or remote areas is small and weak. Frequently, these types of systems depend on thermal diesel generation, and renewable energy integration is a big challenge, because of intermittent and fluctuant patterns of natural sources such as solar irradiation and wind speed especially. Energy Storage Systems-ESS use as a solution to grid stabilization and reduction of operational costs by optimization the use of renewable resources principally. By this study it will determine the power capacity of energy storage facilities by size, to flexible and maximize renewable energy penetration, reducing current operational costs and increasing the reliability of San Cristobal Microgrid. For that, it will select a computer tool to determine the optimal size of energy storage systems.

      • Numerical simulation of sediment transport and bedmorphology around Gangjeong weir in Nakdong river

        Moreno, Carlos Serrano 서울대학교 대학원 2014 국내석사

        RANK : 247359

        Scour around hydraulic structures is one of critical problems in hydraulic engineering: under prediction of scour depths can lead to costly failures in the structure, while over prediction can result in unnecessary construction costs. Unfortunately, up-to-date empirical scour prediction methods and equations based on laboratory data are not always accurate enough and able to reproduce field conditions. Because of physical scales and fluid properties, lab-scale models should be derived from field conditions according to the Hydraulic Similitude Laws. However, unlike physical models, computational fluid dynamics (CFD) tools can perform using real field dimensions and operating conditions to predict turbulent flows and sediment scour. Since the completion of the Four Major Rivers Restoration Project, several new weirs have been installed in the main Korean streams, sediment deposition and erosion around such structures have became a major issue in such rivers. This study is to modify and apply an open source CFD software package, the TELEMAC, to simulate sediment transport and bed morphology around Gangjeong weir in Nakdong River. The real bathymetry of the river and the geometry of weir have been implemented in the numerical model. Numerical results have been validated against available field observations and empirical formulas from literature to predict maximum eroded depths of scour near hydraulic structures. The highest change in bed morphology occurs when the gates are fully opened during flooding. A scour hole is formed behind the weir gates.A reasonably good agreement has been observed between the simulated result and the measured river bathymetry after construction of the weir. The mechanism of formation of the scour hole qualitatively follows Hoffmans' theory, where a strong erosion of the vertical direction is followed by the longitudinal propagation of the hole to the downstream, as well as the amount of eroded sediment decreases reaching to an equilibrium situation. 수공구조물 주변의 세굴 현상은 오늘날 수공학 분야가 다루는 주요한 문제 중 하나이다. 세굴 깊이에 대한 과소평가는 구조에 심각한 문제를 일으키는 반면 과대평가는 불필요한 건설 비용을 유발한다. 하지만 현재까지의 실험에 기초한 세굴 예측 기법과 공식은 현장 조건을 재현할 만큼 정확하지 않다. 축적과 유체 특성에 인해, 상사법칙을 만족하는 현장 조건을 실내모형에 반영해야 한다. 하지만 전산유체역학(CFD)을 이용한 미지수 예측 방법은 실내시험보다 물리적/운동적 제약조건이 적은 편이며, 다양한 설계 변수에 따른 계산을 수행할 수 있고 준경험식에 비해 정확한 계산이 가능하다. 4대강 정비 사업 완공 이후, 주요 하천에 건설된 보 주변의 퇴적ᆞ침식 현상이큰 화제가 되고 있다. 본 연구에서는 낙동강의 강정보 주변의 지형변화를 평가하기 위하여 전산유체역학 소프트웨어 TELEMAC을 사용하였다. 수치 모형에는 강과 구조물의 실제지형공간정보를 반영하였으며, 현장관측 자료와 경험식을 이용하여 그 결과를 비교, 분석하였다. 가장 많은 지형변화는 보 수문이 완전히 개방되는 홍수기에 나타났다. 이는 수문 뒤로 세굴공이 형성되기 때문이다. 수치모델을 이용한 계산결과와 현장 실측된 자료가 일치하였으며, 세굴공 형성의 메커니즘은 호프만 이론을 따랐다.

      • Influence of crime prevention through environmental design (CPTED) on victimization and fear of crime in pedestrians in Panama City

        CARDENAS MORENO, Julio Amador University of Seoul, International School of Urban 2021 국내석사

        RANK : 247358

        라틴 아메리카의 많은 국가들과 같이 파나마에서도 불안정이 계속해서 심화되고 있다. 이는 무엇보다 소득, 사회적 지위, 정치 지도자, 공공 장소의 조건과 같은 다양한 요인들과 관련된 것으로, 파나마에서는 인구의 66%가 거리를 다니는 것에 불안을 느끼며 거리의 58.8%는 야간에 충분한 조명을 제공하지 못하고 있다. 본 연구는 환경설계를 통한 범죄예방(CPTED)을 기본 이론으로 하고 있으며, 이에 대하여 도시적, 건축적 환경 설계를 통한 범죄 예방 차원에서 접근한다. 본 연구는 파나마시티에서 범죄 우려 및 피해에 대한 보행자의 인식을 평가하기 위해 CPTED, 피해, 범죄 우려의 관계를 연구했다. 범죄 우려를 포함한 CPTED와 피해 사이의 관계를 파악하기 위한 분석 방법론으로 구조 방정식 모델(SEM)을 선택했다. 본 연구에 사용된 데이터는 실증적인 방법과 설문조사를 통해 산출되었으며, 설문 문항은 SEM을 수행하는데 필요한 데이터 유형인 5점 리커트 척도를 사용하여 34개의 질문으로 구성되었다. 설문지는 구글 설문지 플랫폼을 통해 온라인으로 배포하였다. 수집된 데이터는 R-Studio를 사용하여 분석되었으며, 본 논문의 결과는 2005년 Minnery 및 Lim의 결과와 일치한다. 본 연구의 결과에 따르면 CPTED는 보행자의 피해 및 범죄 우려와 직접적으로 관련이 있다. 결론적으로 CPTED는 보행자의 피해와 범죄 우려를 줄이는 데 효과적이다. 주요어: CPTED, 보행자, 피해, 범죄 우려, SEM In Panama as many other countries in the Latin American region the levels of insecurity increase constantly, this increment of insecurity is related to different factors as income, social status, political leaders, the conditions of public spaces among other factors. In Panama, 66 percent of the population feel insecure walking in their own neighborhood and 58.8 percent of the streets does not possess appropriate illumination during the nighttime. Crime prevention through environmental design (CPTED) is the base theory for this research, this approach consists in prevent crime through the urban and architectural design of the environment. This research studied the relationship between CPTED, victimization, and fear of crime, in order to evaluate the pedestrian perception of fear of crime and victimization in Panama City. The structural equation model (SEM) was selected as the methodology for analysis to identify the relationship between CPTED with fear of crime and victimization. The data for this study is empirical and it was generated from survey questionnaires survey, the survey was distributed online using the platform Google Forms, it consisted of 34 questions using 5 points Likert scale which the necessary type of data to conduct SEM.   The collected data was analyzed using R-Studio to run the analysis, the obtained results of this dissertation are consistent with Minnery and Lim findings in 2005. The results of this research showed that CPTED is directly related negatively to victimization and fear of crime in pedestrians. Concluding, that CPTED is effective in reducing fear of crime and victimization in pedestrians. Key terms: CPTED, Pedestrian, victimization, fear of crime, SEM

      • (The) effects of 6 weeks Zumba Fitness Program on oscillatory brain activity and cognition in women between 57-90 years old

        Pardo Moreno, Diana Marcela 서울대학교 대학원 2020 국내석사

        RANK : 247358

        Electrical activity of the brain has been studied throughout time and measured using the electroencephalogram (EEG). Studies reveal that exercise has been related to changes in cortical activity, however, the number of researches related with dance and fitness dance-based programs effects on brain activity are very few. The aim of the present study was to examine the effects of 6 weeks Zumba fitness program on oscillatory brain activity and on cognition in women between 57 – 90 years old. Thirty voluntary healthy women, between 57 – 90 years old, members of the adult group "Taller del maestro" of Villa Delia neighborhood located in the city of Bogota - Colombia participated in this study. Participants were randomly assigned, in two groups, Zumba group (n=15) and Control group (n=15). Zumba group women participated during six consecutive weeks, two times per week in a Zumba class, Participants EEG activity was measured before 6 weeks Zumba workout, during activity and immediately after last Zumba class. Also, cognitive test for working memory and attention were performed before and after 6 weeks. Results demonstrates increases in beta absolute power during the stages during and after compared to before measurement. However, no significant results were found in absolute alpha band power during the stages after 6 weeks Zumba program and during session in the Zumba group, these findings suggest increases in EEG beta band power reflect grater cortical activations during and after Zumba, while alpha increases can be due to fatigue or decreased anxiety. This study also showed that 6 weeks of Zumba Fitness program generate positive effects in working memory. On the other hand, taking into account that control group did not underwent any type of treatment, results of brain activity and cognition did not show significant differences before VS after 6 weeks for this group. Additionally, in order to verify data and compare beta wave activity during a Zumba session with control group participants, Motor imagery (MI) test was performed for both groups after 6 weeks. Results showed greater brain activity in beta brain wave in the Zumba group, related to a greater cognitive capacity of attention and working memory while the control group presented low values which can be associated with a lower cognitive capacity.

      • Exploring social media users' engagement based on user-generated images with the physical, natural, and amenity servicescape dimensions : focusing on cafés in Seoul, South Korea

        Alvarez Moreno, Gabriela Alexandra 한양대학교 대학원 2023 국내석사

        RANK : 247358

        Society has managed to incorporate new technologies into daily lifestyles. People stay up to date with the lives of their friends and family by looking into each other’s social media profiles. Subsequently, when they see other people’s social media pages they get ideas of new places to go and new experiences to have. Nowadays customers have multiple options of places to visit, so for café businesses to stay afloat in the overflowing market, they must understand how social media users are behaving toward their posts. In order to analyze the engagement (likes and comments) generated by posts that highlight the physical, natural, and amenity servicescape dimensions, this study utilizes user-generated content (UGC) from the Instagram hashtags of 46 cafés in Seoul, South Korea. For that purpose, this thesis performs a clustering analysis to define the servicescape dimensions present in the dataset (the aforementioned physical, natural, and amenity servicescape dimensions) and subsequently analyses the likes and comments of the images of each cluster to determine if there is a significant difference between the engagement obtained by each cluster. The findings of this thesis suggest that the presence of the physical and natural servicescape dimensions in Instagram posts tends to show a higher number of likes and comments than other combinations of the physical, natural, and amenity dimensions. However, the grouping of the physical and amenity dimensions has the tendency to show a higher average of comments than the rest of the groupings. This thesis is done in hopes that it helps future researchers advance the field of servicescape as viewed through social media, and additionally, that both café owners and designers can use this information to create design strategies that have the potential to be successful at obtaining high levels of engagement on social media.

      • Factors affecting rural community participation in community development projects driven by Babahoyo technical university : case on San Juan, Ecuador

        Brito, Yahaira Lisbeth Moreno Graduate School of Government, Business and Entrep 2022 국내석사

        RANK : 247357

        Community participation is commonly deemed as pivotal in the project success. It is normally seen as a way of ensuring project effectiveness, but less attention has been directed to identifying what kind of factors are affecting or related in the San Juan villager’s participation. This research aims to identify the main features affecting participation rates in community development projects driven by Babahoyo Technical University in Ecuador. The theoretical constructs supported in diverse studies about community participation addressed 19 variables gathered in four groups: socio-demographic features, university management, sociocultural perspective, and community capacity. The questionnaire included 56 questions on liker scale and seven categorical (control variables). After the reliability and validity test, only 44 measurement variables grouped in 11 latent variables and seven control variables were included in the final analysis. Overall, findings show that determinant factors in community participation in CDP driven by Babahoyo University are gender, education level, home-distance, community cooperation, and policy knowledge. Reported outcomes demonstrate that San Juan community participation in CDP directly relates to socio-demographic factors. Additionally, Community capacity factors outcomes are more often associated with increased community participation. That suggests that community participation is a complex process strongly influenced by San Juan villager features and their capacity to face community development issues. To draw out a better understanding of community participation, more robustly designed studies include different project design stages and not only the implementation stage. 커뮤니티 참여는 일반적으로 프로젝트 성공의 핵심으로 간주됩니다. 일반적으로 프로젝트 효율성을 보장하는 방법으로 간주되지만 어떤 종류의 요인이 San Juan 마을 주민의 참여에 영향을 미치거나 관련이 있는지 식별하는 데 덜 관심을 기울였습니다. 이 연구는 에콰도르의 Babahoyo Technical University 에서 주도하는 커뮤니티 개발 프로젝트의 참여율에 영향을 미치는 주요 특징을 식별하는 것을 목표로 합니다. 지역사회 참여에 대한 다양한 연구에서 지원되는 이론적 구성은 사회-인구학적 특징, 대학 관리, 사회문화적 관점 및 지역사회 역량의 4 개 그룹에서 수집된 19 개 변수를 다루었습니다. 설문지는 라이커 척도에 대한 56 개의 질문과 7 개의 범주형(제어 변수)을 포함했습니다. 신뢰도 및 타당도 검사 후 11 개의 잠재변수와 7 개의 통제변수로 분류된 44 개의 측정변수만을 최종분석에 포함시켰다. 전반적으로 연구 결과에 따르면 Babahoyo University 가 주도하는 CDP 의 커뮤니티 참여 결정 요인은 성별, 교육 수준, 집과의 거리, 커뮤니티 협력 및 정책 지식입니다. 보고된 결과는 CDP 에 대한 San Juan 커뮤니티 참여가 사회-인구학적 요인과 직접적인 관련이 있음을 보여줍니다. 또한 커뮤니티 역량 요인 결과는 커뮤니티 참여 증가와 더 자주 관련됩니다. 이는 커뮤니티 참여가 San Juan 마을 주민의 특징과 커뮤니티 개발 문제에 직면할 수 있는 능력에 크게 영향을 받는 복잡한 과정임을 시사합니다. 지역 사회 참여에 대한 더 나은 이해를 이끌어 내기 위해보다 강력하게 설계된 연구에는 구현 단계뿐만 아니라 다양한 프로젝트 설계 단계가 포함됩니다.

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