괄루근의 Glycosidase들에 대한 연구

류병호,양승택,조은정,김동수,문윤희,김희숙 慶星大學校 1996 論文集 Vol.17 No.2

Several glycosidase activities, α-galactosidase, β-galactosidase and β-fucosidase and their isozymes were founded in Trichosantes kirilowii root, Trichosantes radix. The activities of α-glucosidase, β-glucosidase and α-fucosidase were also assayed but were not detected. Those glycosidases were isolated and partial purified glycosidases by acid-treated Sepharose-6B, anion exchange Mono-Q HPLC, DEAE-Sephacel, butyl-toyopearl hydrophobic column chromatographies. However, these glycosidases need to be purified more homogeneous and characterized.

Application ot the Nonlinear System of Star Diagram

최문술,류문희 군산대학교 1989 論文集 Vol.16 No.-

속도방정식인 Schlogl model에 대한 retarded n-point 함수를 명확하게 나타내었다.어떤 면에서는 이러한 방법을 다른 비선형계에 정확한 형태로 적용할 수 있다. The retarded n-point function of a Schl□gl model which is rate expression is represented explicitly.In some cases this method allows one to apply to the other nonlinear system in explicit form.

오리엔탈나리의 球根 收穫時期와 低溫處理 期間이 生育에 미치는 影響

金熙峻,金正萬,金致善,柳汀,崔泳根,文炳永 한국화훼연구회 2001 화훼연구 Vol.9 No.1

This study was carried out to determine the effect of the duration of chilling treatment and the lifting time of bulbs on growth of Lilium Oriental hybrids for product of cut flower in the type of forcing or semiforcing culture in Korea. "Casablanca", "Acapulco" and "Le Reve" in lilium Oriental hybrids were used in these experiment and carried out from 1999 to 2000 in Iksan, Korea. The bulbs for experiment were lifted on August 27, September 27 and October 27 and the all of them were chilled at 5±1℃ for 0,8,10,12 weeks. Development of the bulbs lifted on October 27 was superior than that of lifted bulbs on August 27 in the three cultivars. The bulbs lilted lately and chilled for a long time were sprouted more earlier and grown faster. In order to increase bulb sprouting until 100%, the duration of chilling treatment of bulbs was need for 12 weeks on August 27, 10 weeks on September 27 and above 8 weeks on October 27. The flowering according to the duration of chilling treatment and the lifting time of bulbs was same above. Consequently, the bulbs should be chilled at 5±1℃ for chilled for 10 weeks at late September and 8 weeks in producted bulbs at late October in earlyㆍMidseason variety fur product of cut flower hut bulbs of late variety should be chilled for 12 weeks at late September and 10 weeks at late October.

성인원외폐렴의 원인미생물에대한 전향적 다기관 연구 : 성인원외폐렴의 원인으로 세균의 역할을 중심으로

우준희,강재명,김양수,신완식,류진홍,최정현,김양리,정희진,어수택,박춘식,정문현,정기석,이찬주,류지소 대한감염학회 2001 감염 Vol.33 No.1

Background : Communite-acquired peumonia (CAP) is one of the leading causes of mortality and morbidity. Despite progress in diagnostic techniques and treatments, management of pneumonia remains challenging, because the precise etiology remains uncertain in as many as 49% of cases. The limitaions of identifying etiologic agents make it necessary to use empiric antibiotics in almost all patients, and furthermore emergence of antibiotic-resistant organisms pose difficulties to the selection of an empiric antibiotic regimen. For the optimal choice of empirical antibiotics, we should know the frequency of etiologic agents and antimicrobial resistance rates in the community. Methods : A prospective multicenter study of community-acquired pneumonia in Korea was carried out between May 1997 and April 2000. The microbiologic diagnosis was based on the results of sputum culture, blood culture and pleural culture. Results : Five hundred eighty eight cases of community-acquired peumonia in 562 patients admitted to the hospitals. The mean age was 59.9 with male predominance (58.3%), and 370 (63%) had underlyin gillness. The etiologic agents were identified in 38.3%, and the list of individual agents, in decreasing order, was Streprococcus pneumoniae (21.7%), Klebsiella pneumoniae (14.8%) Pseudomonas aeruginosa (9.8%), Staphylococcus aureus (9.5%), viridans group streptococci (5.7%), Enterobacter cloacae (4.2%), Hemophillus Influenza (3.8%), The rates of admission to the intensive care unit was 10.4%. The motality was 7.1%. Susceptible rates of S. pneumoniae to penicillin was 36.6% and showed multidrug resistant. Forty percents of S. aureus were methicillin-resistant S. aureus. K. penumoniae were susceptible to cephalosporin and quinolone. Conclusion : In Korea, S.pneumoniae is the most important agent causing community-acquired pneumonia. Susceptible rates of S. pneumoniae to penicillin was 36.6% and showed multidrug resistant. Gram negative bacteria such as K. pneumoniae, P. aeruginosa showed high incidence when compared with that of other countries. Most of them had underlying diseases including bronchiectasis and chronic obstructive pulmonary diseases. (Korean J Infect Dis 33:1∼7, 2001)

ASTM F813-07 의료용디바이스를 위한 생체재료의 직접촉 세포배양측정에 대한 표준지침서

김순희 ( Soon Hee Kim ),백미옥 ( Mi Ock Baek ),소정원 ( Jung Won So ),노혜원 ( Hye Won Roh ),이나리 ( Na Ri Lee ),김문석 ( Moon Suk Kim ),유규하 ( Gyu Ha Ryu2,),조양하 ( Yang Ha Cho ),이승진 ( Seung Jin Lee ),강길선 ( Gilson Khang 한국조직공학·재생의학회 2007 조직공학과 재생의학 Vol.4 No.4

We provided standard practice for direct contact cell culture evaluation of materials for medical devices in this report. This practice introduced a reference method of direct contact cell culture testing. This method of direct contact cell culture may be used in the construction of medical materials and devices for test the cytotoxic potential of materials. Researcher for medical devices could use this practice to compare other cytotoxicity tests or directly to evaluate materials. Cell or materials could be sometimes modified depending on use in offered extend of this practice.

扶土灣의 環境과 資原生物學的 硏究

金重來,柳文熙,鄭義泳,崔文術 群山大學校自然科學硏究所 1986 自然科學硏究 Vol.1 No.-

扶土灣의 海洋還境 및 生物棲息環境을 비롯하여 植物플랑크톤, 主要水産動物의 生殖生態, 個體群成長, 害敵生物에 의한 白蛤의 斃死, 貝類의 資源量, 主要水産動物의 漁獲量, 그리고 有用海藻類의 生産에 關한 調査硏究 結果를 要約하면 다음과 같다. 1. 榮養鹽으로서 ??-N, ??-N, T-N과 ??-P는 陸水의 영향을 받는 곳에서 0.028∼0.046, 0.0011∼0.0023, 0.038∼0.049와 0.025∼0.035 mg/ℓ로 比較的 높았으나 陸水의 영향을 덜 받는 곳에서 각각 0.026∼0.031, 0.0005∼0.0017, 0.033∼0.043과 0.019mg/ℓ로 비교적 낮았다. 內灣으로 들어올수록 COD는 증가하며, 투명도는 감소하는 것으로 調査되었다. 2. 식물플랑크톤은 매번 1종 내지 3종이 크게 우점하였으며, 6월, 8월, 10월에 가장 큰 우점을 보인 種은 각기 Leptocylindrus danicus, Chaetoceros pseudocurvisetus 및 Chaetoceros rostratus였다. 여타의 해양생태학적 환경에 비하여, 조간대의 식물플랑크톤 군집은 계절변화나 종조성에 있어, 뚜렷한 차이가 있는 것으로 나타났다. 3. 백합, Meretrix lusoria, 바지락, Ruditapes philippinarum 그리고 가무락, Cyclina sinensis는 雌雄異體로서 卵生이다. 4. 백합과 바지락의 生殖周期는 5段階(分裂增殖期, 成長期, 成熟期, 放出期, 그리고 退化 및 休止期)로 區分할 수 있었으며, 가무락은 6段階(分裂增殖期, 成長期, 成熟期, 放出期, 退化期 그리고 回復期)로 區分할 수 있었다. 5. 백합의 産卵은 5月에서 10月까지 일어나며, 産卵盛期는 6月에서 7月이다. 그리고 이들 個體群의 群成熟度는 암, 수 殼長이 35∼40mm인 個體는 50% 以上이었고 殼長이 50∼60mm인 個體는 100%로 全個體가 再生産에 참여하였다. 6. 바지락의 産卵은 5月에서 9月까지 일어나며 7, 8月이 産卵盛期이다. 그리고 이들 個體群의 群成熟度(生物學的 最少成熟個體)가 50% 以上인 個體의 殼長은 20∼25mm이었고 全個體가 再生産에 참여하여 群成熟度 100%가 되는 個體들의 殼長의 크기는 35∼40mm 이었다. 7. 가무락의 産卵은 7月에서 9月까지 일어나며 7, 8月이 主産卵期이다. 8. Von Bertalanffy의 방정식에 의한 殼長과 全重量에 對한 成長曲線은 다음과 같다. 1) 백합 : ??, ?? 2) 바지락 : ??, ?? 3) 가무락 : ??, ?? 9. 穿孔腹足類인 큰구슬우렁, Neverita didyma에 依한 白蛤의 穿孔의 位置는 다양하나 大部分은 比較的 一定한 位置인 殼頂部位에 있었다. 그리고 구멍의 內徑과 外徑의 크기는 貝類의 殼長에 비례하였다. 10. 큰구슬우렁, N. didyma의 포식율은 實驗室內에 調査한 結果 주간보다 야간에 더 컸고, 큰구슬우렁 1마리가 하루에 평균 0.2個體(殼長 20mm 以上)를 穿孔하여 포식하였다. 11. 扶土灣內의 年間 生産可能量은 白蛤이 ??, 가무락 ?? 그리고 바지락 ??으로 推定된다. 12. 김 生産量과 氣象要因과의 관계는 매우 密接하며 특히 養殖期間中의 적당한 風波는 김 生産에 크게 效果的이다. 13. 김의 年間生産量은 乾海苔로 約 439,500束으로 推定된다. 14. 主要水産動物(貝類除外)의 年間漁獲量은 約 38,430kg이며 이 中 魚類가 13種에 30,740.5 kg으로서 約 80%를 占有하고 單一魚種으로는 학공치가 으뜸으로 30.5%를 차지한다. Environmental and resources biological studies were performed aiming to the preservation of the resources biota in Pusa Bay from April to November 1986. 1. The nutrient concentrations of ??-N, ??-N, T-N and ??-P in inquiry area affected by the inflowing inland water were 0.028-0.046, 0.0011-0.0023, 0.038-0.049 and 0.025-0.035 (mg/1), respectively. However in the area not affected by the inland water the nutrient concentrations were 0.026-0.031, 0.0005-0.0017, 0.033-0.043 and 0.019, respectively. 2. Great dominance were shown by one to three phytoplankton species at each sampling time. The most dominants were Leptocylindrus danicus, Chaetoceros pseudocurvisetus, and Chaetoceros rostratus in June, August, and October, respectively. Intertidal phytoplankton community were found to be quite distinct in its seasonal succession and also in its species composition from that of the other temperate sea areas. 3. Sexualities of Meretrix lusoria, Ruditapes philippinarum and Cyclina sinensis are dioecious, and the species are oviparous. 4. The reproductive cycles of M. lusoria and R. philippinarum can be classified into five successive stages (multiplicative, growing, mature, spent, and degenerative and resting stage), while those of Cyclina sinensis can be classified into six successive stages (multiplicative, growing, mature, spent, degenerative, and recovery stage). 5. Spawning of Meretrix lusoria occurs from May to October with a peak from June to July, and the percentages of the first maturity of female and male clams are over 50% in 35∼40 mm and 100% in 50 ∼60 mm of the shell length. 6. Spawning of Ruditapes philippinarum occurs from May to September with a peak spawning in July-August, and the percentages of the first maturity (biological minimum size) of these populations are over 50% in 20∼25 mm, and 100% in 35∼40 mm of the shell length. 7. Spawning of Cyclina sinensis occurs from July to September with a peak spawning in July-August. 8. Growth curves for the shell length and total weight fitted to von Bertalanffy's equation were expressed as follows: 1) Meretrix lusoria : ?? ?? 2) Ruditapes philippinarum : ?? ?? 3) Cyclina sinensis : ?? ?? 9. The location of the holes on the valves of Meretrix lusoria by boring snail, Neverita didyma varied widely, while most of holes on the valves were located at relatively definite position (umbo position) on the valves. The inner and outer diameter of the holes varied in proportion to the shell length of the clam. 10. Predation rates of Nerverita didyma was much greater at night than during the day in the laboratory, an average of 0.2 individual of clam was drilled and consumed per snail per day. 11. The amount of annual yield of Meretrix lusoria, Ruditapes philippinarum and Cyclina sinensis were estimated to be about 726.40M/T, 32.31M/T and 23.91M/T, respectively. 12. The productivity of Porphyra species is closely related with atomospheric factors in the culture ground through the seasons in 1985. The wind among the factors seems to have a good effect on the growth and production of Porphyra. 13. The annual yield of Porphyra species was estimated to be about 439,500 bundles by dried-laver. 14. The annual catch of commercial species of fisheries animals (except shell-fishes) was presumed to be about 38,430 kg and the fishes among them, including 13 species, was calculated to 30,740.5 kg, which dominated over 80% of total fishing amounts. The most important species of fishes was quantitatively Hemirhamphus sajori, whose catch was inferred to 3,300 kg at a fish-farm (10ha in scale) and 30.5% of the totality, followed by Platycephalus indicus.

익산시 수질오염지역 주민 모발내의 중금속 함량에 관한 연구

유일수,류문희,최민규 원광대학교 환경과학연구소 2000 環境科學硏究誌 Vol.9 No.1

Platinum sensitive 재발성 난소암에서 Carboplatin/Paclitaxel의 효과

장혜진,유희석,임윤경,문세희,장기홍,이정필 대한부인종양 콜포스코피학회 2003 Journal of Gynecologic Oncology Vol.14 No.4

목적 : 재발성 난소암에서 platinum 감수성을 기준으로 2차 항암요법으로 사용한 Carboplatin과 Paclitaxel의 효과를 알아보기 위하여 본 연구를 진행하였다. 연구방법 : 1998년 1월 1일부터 2003년 8월 31일까지 만 5년 8개월 간 아주대학교병원 산부인과에 재발성 난소암으로 입원한 환자 중에서 최초 수술로 난소암을 확진한 후 Cisplatin 또는 Carboplatin 등 platinum 제재가 포함된 항암치료를 받은 후 6개월 이후에 재발한 환자 29명을 대상으로 Carboplatin은 5 AUC를 그리고 Paclitaxel은 175 mg/m^(2)를 투여하였으며, 최소 3회에서 최고 16회까지 투여하였다. 치료효과의 판정은 CT, MRI, Ultrasonography등 영상진단상 암종괴의 크기가 완전히 소실되었을 경우를 완전반응(complete response)으로, 50%이상 감소되었을 경우를 부분반응(partial response)으로, 50% 미만으로 감소되었거나 20% 미만 증가하였을 경우를 불변(stable disease)으로, 그리고 20% 이상 증가되었을 경우를 진행(progressive disease)로 정의하였다. 결과 : 대상 환자에서 완전반응은 6명(21%), 부분반응은 11명(38%), 그리고 불변은 5명(17%)로 전체 반응율 (overall response rate)은 59% 그리고 임상적 유용성(clinical benefit=CR+PR+SD)은 76%이었다. 결론 : 재발성 난소암환자에서 기왕에 사용한 Platinum 제재에 민감한 환자에서는 Carboplatin과 Paclitaxel의 병행 투여가 효과적이었다. Objective : To ascertain the effect of Carboplatin and Paclitaxel combination as a second line regimen for recurrent ovarian carcinoma based on platinum sensitivity. Methods : From January 1st, 1998 through to August 31st, 2003, 29 patients were retrospectively reviewed who had previously underwent primary cytoreductive surgery and adjuvant chemotherapy with Cisplatin or Carboplatin for ovarian carcinoma, and who had later recurred during follow up. All 29 patients had recurred at least 6 months after therapy, and thereafter received second line chemotherapy consisting of Carboplatin (5 AUC) and Paclitaxel (175 mg/㎡) for a minimum of least 3 cycles and a maximum of 16 cycles. Treatment efficacy was assessed by imaging studies including Computerized tomography, Magnetic Resonance Imaging, or Ultrasonography. Complete response was determined as complete disappearance of tumor, partial response at least than 50% reduction in tumor volume, stable disease as less than 50% reduction or less than 20% increase in tumor volume, and progressive disease as more than 20% increase. Results : There was complete response in 6 (21%) patients, partial response in 11 (38%), stable disease in 5 (17%), showing an overall response rate of 59%. The clinical benefit (CR+PR+SD) was 76%. Conclusion : The combination of Carboplatin and Paclitaxel administration seems to be an effective mode of second line therapy in patients with recurrent ovarian carcinoma who had been sensitive to prior platinum based chemotherapy.

대두단백질 가수분해물의 특성

김동수,양승택,문윤희,류병호,김희숙,염동민,김진목 慶星大學校 1998 論文集 Vol.19 No.1

The present study was conducted to elucidate some characteristics of soy protein hydrolysates. The soy protein was obtained from defatted soy power by the treatment of n-hexan. The hydrolysates of soy protein were prepared from hydrolysis by bromelian and pepsin with the two kinds of samples of precooking for 30 min in steam oven and noncooking, and fractionated through Bio-gel P-4 column chromatography. Fractions of hydrolysates separated on the Bio-gel column chromatography were carried out to SDS-PAG electrophoresis, resulting to show some different bends from 66kD to 14.2kD of molecular weight. In addition, the fractions showed different activities of superoxide dismutase. From these results, it was found that the hydrolysates could be available to use in a lot of food industrial fields as new functional food materials because the hydrolysates had different characteristics in electrophoresis and could increase the activity of superoxide dismutase.

복어卵巢 鹽藏品 製造中의 化學的 成分 및 毒性의 變化

김동수,박지현,류병호,양승택,문윤희,김희숙 慶星大學校 1993 論文集 Vol.14 No.4

Attempts were made to investigate the levels of chemical components and detoxification of the salted puffer overies, Fugu xanthopterus, "Ggachibog" and Fugu rubripes rubripes, "Chambog", processing of solid salt mixtures containing sodium bicarbonate, during storage period. Fresh ovaries of the puffers were divided into three portions, sprinkled with a solid salt mixture containing 0,1 or 2% NaHCO₃, and were stored at room temperature for 12 weeks. The samples were examined at each step and assayed for pH, VBN, amino-N, toxicity and thin- layer chromatogram pattern. The results were summarized as follows: The values of pH were appeared to be different with the added amount of siodium bicarbonate and the values of pH were over 7.0 in a salt mixture containing 2% NaHCO₃. The change of VBN value was also revealed about 85mg/100g in A division and 90-100mg/100g in both B and C, respectively, in the salted ovary of puffer "Ggachibog" from 8 weeks. In case of salted product from the "Chamboh" ovary, it had slightly lower value than that of "Ggachibog" ovary. Also, the value of amino-N was increased until 4-6 weeks and was slowly down after the weeks in all samples. All salted products were found to lose most of the toxicity during storage period when the salt containing NaHcO₃was used for sprinkling. In the case of raw ovaries being weak toxicity below 5.0 MU/g or 24.1 MU/g, the toxicity of salted product was dropped near the level to be edible. Therefore there was significant detoxification of the puffer ovaries by the salt mixture containing sodium bicarbonate. Finally, the toxins isolated from each raw puffer ovary were detected to be TTX-related components by thin-layer chromatogrphy.