Ovarian Masses: Is Multi-detector Computed Tomography a Reliable Imaging Modality?

Khattak, Yasir Jamil,Hafeez, Saima,Alam, Tariq,Beg, Madiha,Awais, Mohammad,Masroor, Imrana Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.4

Background: Ovarian cancer continues to pose a major challenge to physicians and radiologists. It is the third most common gynecologic malignancy and estimated to be fifth leading cancer cause of death in women, constituting 23% of all gynecological malignancies. Multi-detector computed tomography (MDCT) appears to offer an excellent modality in diagnosing ovarian cancer based on combination of its availability, meticulous technique, efficacy and familiarity of radiologists and physicians. The aim of this study was to compute sensitivity, specificity, positive and negative predictive values and diagnostic accuracy of 64-slice MDCT in classifying ovarian masses; 95% confidence intervals were reported. Materials and Methods: We prospectively designed a cross-sectional analytical study to collect data from July 2010 to August 2011 from a tertiary care hospital in Karachi, Pakistan. A sample of 105 women aged between 15-80 years referred for 64-MDCT of abdomen and pelvis with clinical suspicion of malignant ovarian cancer, irrespective of stage of disease, were enrolled by non-probability purposive sampling. All patients who were already known cases of histologically proven ovarian carcinoma and having some contraindication to radiation or iodinated contrast media were excluded. Results: Our prospective study reports sensitivity, specificity; positive and negative predictive values with 95%CI and accuracy were computed. Kappa was calculated to report agreement among the two radiologists. For reader A, MDCT was found to have 92% (0.83, 0.97) sensitivity and 86.7% (0.68, 0.96) specificity, while PPV and NPV were 94.5% (0.86, 0.98) and 86.7% (0.63, 0.92), respectively. Accuracy reported by reader A was 90.5%. For reader B, sensitivity, specificity, PPV and NPV were 94.6% (0.86, 0.98) 90% (0.72, 0.97) 96% (0.88, 0.99) and 87.1% (0.69, 0.95) respectively. Accuracy computed by reader B was 93.3%. Excellent agreement was found between the two radiologists with a significant kappa value of 0.887. Conclusion: Based on our study results, we conclude MDCT is a reliable imaging modality in diagnosis of ovarian masses accurately with insignificant interobserver variability.

Role of Cardiac MRI in Complicated Cardiac Defects

Khan Yasir,Pervez Mohammad Bin,Fatimi Saulat 아시아심장혈관영상의학회 2019 Cardiovascular Imaging Asia Vol.3 No.3

Sinus of Valsalva rupture is a rare condition that results in aortocardiac shunt. Although the investigation of choice is transthoracic echocardiography, the gold standard is cardiac catheterization. We present a case of a young male with acute onset heart failure. Acute coronary syndrome blood workup was unremarkable. Transthoracic and transesophageal echocardiography revealed mild right atrial dilation with a Gerbode shunt. Right heart catheterization showed left to right shunt suggestive of acquired acute onset shunting. Eventually, a cardiac MRI revealed a ruptured sinus of Valsalva opening into the right atrium as the cause. The patient was taken to the operation theater where an aortobicaval bypass was established. A horizontal aortotomy revealed a sinus of Valsalva aneurysm arising from the right coronary sinus which was repaired using a Dacron patch. Postoperative transthoracic echocardiography showed no defect. In summary, cardiac MRI is an excellent tool for establishing a definite diagnosis for complicated cardiac defects.

A Review on Venom Enzymes Neutralizing Ability of Secondary Metabolites from Medicinal Plants

Singh, Pushpendra,Yasir, Mohammad,Hazarika, Risha,Sugunan, Sunisha,Shrivastava, Rahul KOREAN PHARMACOPUNCTURE INSTITUTE 2017 Journal of pharmacopuncture Vol.20 No.3

Objectives: Medicinal plants are vital sources of bioactive compounds that are useful for the treatment of patients with snake bites or are indirectly applicable for boosting the effects of conventional serum therapy. These plants are being used traditionally by local healers and tribes for the treatment of patients with snake bites and therefore can be used as an alternative against snake envenomation. Scientifically, using the secondary metabolites of plants to neutralize venom enzymes has an extra benefit of being based on traditional knowledge; also, the use of such metabolites for the treatment of patients with snake bites is cheaper and the treatment can be started sooner. Methods: All the available information on various secondary metabolites exhibiting venom neutralizing ability were collected via electronic search (using Google books, Pubmed, SciFinder, Scirus, Google Scholar, and Web of Science) and articles of peer-reviewed journals. Results:Recent interest in different plant has focused on isolating and identifying of different phytoconstituents that exhibit Phospholipase A2 activity and other venom enzyme neutralizing ability. In this support convincing evidence in experimental animal models are available. Conclusion: Secondary metabolites are naturally present, have no side effect, are stable for a long time, can be easily stored, and can neutralize a wide range of snake enzymes, such as phospholipase A2, hyaluronidase, protease, L-amino acid oxidase, 5'nucleotidase, etc. The current review presents a compilation of important plant secondary metabolites that are effective against snake venom due to enzyme neutralization.

A Review on Venom Enzymes Neutralizing Ability of Secondary Metabolites from Medicinal Plants

Pushpendra Singh,Mohammad Yasir,Risha Hazarika,Sunisha Sugunan,Rahul Shrivastava 대한약침학회 2017 Journal of pharmacopuncture Vol.20 No.3

Objectives: Medicinal plants are vital sources of bioactive compounds that are useful for the treatment of patients with snake bites or are indirectly applicable for boosting the effects of conventional serum therapy. These plants are being used traditionally by local healers and tribes for the treatment of patients with snake bites and therefore can be used as an alternative against snake envenomation. Scientifically, using the secondary metabolites of plants to neutralize venom enzymes has an extra benefit of being based on traditional knowledge; also, the use of such metabolites for the treatment of patients with snake bites is cheaper and the treatment can be started sooner. Methods: All the available information on various secondary metabolites exhibiting venom neutralizing ability were collected via electronic search (using Google books, Pubmed, SciFinder, Scirus, Google Scholar, and Web of Science) and articles of peer-reviewed journals. Results: Recent interest in different plant has focused on isolating and identifying of different phytoconstituents that exhibit Phospholipase A2 activity and other venom enzyme neutralizing ability. In this support convincing evidence in experimental animal models are available. Conclusion: Secondary metabolites are naturally present, have no side effect, are stable for a long time, can be easily stored, and can neutralize a wide range of snake enzymes, such as phospholipase A2, hyaluronidase, protease, L-amino acid oxidase, 5’nucleotidase, etc. The current review presents a compilation of important plant secondary metabolites that are effective against snake venom due to enzyme neutralization.

Snake Venom Phospholipase A2 and its Natural Inhibitors

Rahul Shrivastava,Mohammad Yasir,Ruchi Khare,Manish Kumar Tripath,Rahul Shrivastava 한국생약학회 2020 Natural Product Sciences Vol.26 No.4

Snakebite is a severe medical, economic, and social problem across the world, mostly in the tropical and subtropical area. These regions of the globe have typical of the world's venomous snakes present where access to prompt treatment is limited or not available. Snake venom is a complex mixture of toxin proteins like neurotoxin and cardiotoxin, and other enzymes like phospholipase A2 (PLA2), haemorrhaging, transaminase, hyaluronidase, phosphodiesterase, acetylcholinesterase, cytolytic and necrotic toxins. Snake venom shows a wide range of biological effects like anticoagulation or platelet aggregation, hemolysis, hypotension and edema. Phospholipase A2 is the principal constituent of snake venom; it catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid, which is the precursor of eicosanoids including prostaglandins and leukotrienes. The information regarding the structure and function of the phospholipase A2 enzyme may help in treating the snakebite victims. This review article constitutes a brief description of the structure, types, mechanism occurrence, and tests of phospholipase A2 and role of components of medicinal plants used to inhibit phospholipase A2.

Effects of carbamate pesticides intermediates on Escherichia coli membrane architecture: An in vitro and in silico approach

Pushpendra Singh,Manish Kumar Tripathi,Mohammad Yasir,Ashish Ranjan,Rahul Shrivastava 환경독성보건학회 2021 환경독성보건학회지 Vol.36 No.3

Methyl isocyanate (MIC), a low molecular weight synthetic aliphatic compound, having an isocyanate group (−NCO), has industrial application. In this study, the effects of methyl isocyanate and its mechanism on outer membrane protein of Escherichia coli were observed using experimental and computational methods. In vitro exposure of N-succinimidyl N-methylcarbamate (NSNM) a synthetic analogue of MIC on E. coli to a final concentration of 2 mM was found to affect the growth curve pattern and changes in cell morphology. Molecular docking studies of MIC and NSNM with E. coli outer membrane protein (OmpW, OmpX, OmpF OmpA), and periplasmic domain (PAL) were performed. The in-silico results revealed that outer membrane protein OmpF showed the highest negative binding energy, i.e. ΔG -4.11 kcal/mole and ΔG -3.19 kcal/mole by NSNM and MIC as compared to other proteins. Our study concludes that methyl isocyanate retains lethal toxicity which leads to cell death due to the membrane protein damage of E. coli membrane.

Evaluation of young F1 Lilium hybrids using fluorescence in situ hybridization analysis and morphological attributes

Ramzan Fahad,Hwang Yoon-Jung,Choi Yu Hyeon,Ramzan Yasir,Islam MD Mazharul,Deepo Deen Mohammad,김창길,임기병 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.5

Lilium, an important ornamental crop, requires genetic and phenotypic assessment in the development of new hybrids. Therefore, genetic (using FISH) and morphological studies were conducted on newly developed hybrids belonging to Lilium genomes (Asiatic, Oriental, longiflorum). Morphological results revealed that all F1 hybrids showed significant differences from one another. Triploid Asiatic hybrids (Brunello × Mapira) showed good phenotypic characteristics and longiflorum hybrids (Bright Tower × Snow Queen) expressed good growth characters. The Brunello × Mapira hybrid was especially superior in root length, number of scales, bulb diameter and bulb weight. Chromosomal studies con- firmed that all hybrids were diploid (2x = 24) with 24 chromosomes except the “AA-2” Asiatic hybrid which was triploid (2x = 3x = 36). In our karyotyping results, there was non-significant variation with respect to short arm length and arm length ratio among hybrids, while long arm length and total chromosome length showed significant differences. In the case of 45S signals, the maximum number of 45S rDNAs were found in “Bright Tower × Snow Queen” while the minimum number of 45S signals (2) were found in “Yellow cocotte × Valdisole”. 5S signals were similar in all hybrids except trip- loid Asiatic hybrids. Thus, newly developed young hybrids can be successfully screened using morphological and FISH analysis. FISH karyotyping identified a triploid Asiatic hybrid which was also superior in phenotypic characteristics.

Filter orthogonal frequency-division multiplexing scheme based on polar code in underwater acoustic communication with non-Gaussian distribution noise

Ahmed, Mustafa Sami,Shah, Nor Shahida Mohd,Al-Aboosi, Yasin Yousif,Gismalla, Mohammed S.M.,Abdullah, Mohammad F.L.,Jawhar, Yasir Amer,Balfaqih, Mohammed Electronics and Telecommunications Research Instit 2021 ETRI Journal Vol.43 No.2

The research domain of underwater communication has garnered much interest among researchers exploring underwater activities. The underwater environment differs from the terrestrial setting. Some of the main challenges in underwater communication are limited bandwidth, low data rate, propagation delay, and high bit error rate (BER). As such, this study assessed the underwater acoustic (UWA) aspect and explored the expression of error performance based on t-distribution noise. Filter orthogonal frequency-division multiplexing refers to a new waveform candidate that has been adopted in UWA, along with turbo and polar codes. The empirical outcomes demonstrated that the noise did not adhere to Gaussian distribution, whereas the simulation results revealed that the filter applied in orthogonal frequency-division multiplexing could significantly suppress out-of-band emission. Additionally, the performance of the turbo code was superior to that of the polar code by 2 dB at BER 10-3.