Some Desmids from Garhwal Region of Uttarakhand, India

Misra, Pradeep Kumar,Misra, Purnima,Shukla, Madhulika,Prakash, Jai The Korean Society of Phycology 2008 ALGAE Vol.23 No.3

The present paper consists of 42 taxa belonging to 7 genera of desmids (green algae) collected from two districts of Garhwal region of Uttarakhand (Western Himalayas). The district Haridwar is located 29° 55’to 29° 59’N latitude and 68° 5’to 68° 30’E longitude covering about 2360 km2 area and Dehradun district is situated between 77° 34’to 78° 18’E longitude and 29° 58’to 30° 58’N latitude. Seven genera of desmids are (with number of taxa in parenthesis): Closterium Nitzsch. (9), Cosmarium Corda ex Ralfs (25), Euastrum Ehr. (2), Spondylosium Breb. (1), Micrasterias Ag. (1), Staurastrum Meyen (3), Arthrodesmus Ehr. (1). All these taxa constitute new records for the area. The algal localities are relatively cleaner than those of majority of urban areas. A rich assemblage of desmids shows that water bodies of these hilly areas are still undisturbed and need protection for preservation of algal biodiversity.

Improvement in Clonal Propagation of Hemidesmus indicus R. Br. through Adenine Sulphate

Misra Neeta,Misra Pratibha,Datta S.K.,Mehrotra Shanta The Korean Society of Plant Biotechnology 2003 Plant molecular biology and biotechnology research Vol.5 No.4

A protocol has been developed for rapid large scale clonal propagation of an aromatic endangered medicinal plant, Hemidesmus indicus R. Br. with the elimination of the problems such as premature leaf fall and callus formation during caulogenesis and rhizogenesis. Multiple shoots were induced from shoot tip and nodal explants on Murashige and Skoog (MS) medium supplemented with 1 mg/L Benzylaminopurine (BAP) and 0.5 mg/L Napthaleneaceticacid (NAA). Addition of 15 mg/L adenine sulphate to the above medium checked leaf abscission completely, reduced the time required for caulogenesis and restored morphogenetic potential after several subcultures. The in vitro grown propagules were rooted in 1/2 MS medium supplemented with 2 mg/L Indolebutyric acid (IBA) +1 mg/L NAA and sucrose 0.7% (w/v). Addition of charcoal at 100 mg/L to the rooting medium quickened root initiation with a complete check on callus formation. The effect of sucrose concentration on both caulogenesis and rhizogenesis was also studied. The resultant plantlets were acclimatized and grown in fields where ninety eight percent of the rooted shoots survived and grew normally. The estimation of the secondary metabolite content in the shoots of the regenerated plant and the mother plant indicated that the concentration of the three secondary metabolites lupeol, vanillin and rutin was similar.

Helicobacter pylori Infection and a P53 Codon 72 Single Nucleotide Polymorphism: a Reason for an Unexplained Asian Enigma

Pandey, Renu,Misra, Vatsala,Misra, Sri Prakash,Dwivedi, Manisha,Misra, Alok Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21

Aim: P53, the most commonly mutated tumor suppressor gene in all types of human cancer, is involved in cell cycle arrest and control of apoptosis. Although p53 contains several polymorphic sites, the codon 72 polymorphism is by far more common. There are divergent reports but many studies suggest p53 pro/pro SNP may be associated with susceptibility to developing various cancers in different regions of the world. The present study aimed to find any correlation between H. pylori infection and progression of carcinogenesis, by studying apoptosis and the p53 gene in gastric biopsies from north Indian population. Materials and Methods: A total of 921 biopsies were collected and tested for prevalence of H. pylori by rapid urease test (RUT), imprint cytology and histology. Apoptosis was studied by the TUNEL method. Analysis of p53 gene polymorphism at codon 72 was accomplished by PCR using restriction enzyme BstU1. Observation: Out of 921 samples tested 56.7% (543) were H. pylori positive by the three techniques. The mean apoptotic index (AI) in the normal group was 2.12, while gastritis had the maximum 4.24 followed by gastric ulcer 2.28, gastropathy 2.22 and duodenal ulcer 2.08. Mean AI in cases with gastric cancer (1.72) was less than the normal group. The analysis of p53 72 SNP revealed that p53 (Arg/Arg), (Pro /Arg) variant are higher (40.59% & 33.66%) as compared to p53 pro/pro variant (25.74%) inthe healthy population. Conclusions: The North Indian population harbors Arg or Pro/Arg SNP that is capable of withstanding stress conditions; this may be the reason of low incidence of gastric disease in spite of high infection with H. pylori. There was no significant association with H. pylori infection and AI. However, there is increased apoptosis in gastritis which may occur independent of H. pylori or p53 polymorphism.

Some Desmids from Garhwal Region of Uttarakhand, India

Pradeep Kumar Misra,Madhulika Shukla and Jai Prakash,Purnima Misra 한국조류학회I 2008 ALGAE Vol.23 No.3

The present paper consists of 42 taxa belonging to 7 genera of desmids (green algae) collected from two districts of Garhwal region of Uttarakhand (Western Himalayas). The district Haridwar is located 29° 55’ to 29° 59’ N latitude and 68° 5’ to 68° 30’ E longitude covering about 2360 km2 area and Dehradun district is situated between 77°34’ to 78° 18’ E longitude and 29° 58’ to 30° 58’ N latitude. Seven genera of desmids are (with number of taxa in parenthesis): Closterium Nitzsch. (9), Cosmarium Corda ex Ralfs (25), Euastrum Ehr. (2), Spondylosium Breb. (1), Micrasterias Ag. (1), Staurastrum Meyen (3), Arthrodesmus Ehr. (1). All these taxa constitute new records for the area. The algal localities are relatively cleaner than those of majority of urban areas. A rich assemblage of desmids shows that water bodies of these hilly areas are still undisturbed and need protection for preservation of algal biodiversity.

Image-Guided Fine Needle Cytology with Aspiration Versus Non-Aspiration in Retroperitoneal Masses: Is Aspiration Necessary?

Rajiv Kumar Misra,Shaila Mitra,Rishav Kumar Jain,Shilpa Vahikar,Archana Bundela,Purak Misra 대한병리학회 2015 Journal of Pathology and Translational Medicine Vol.49 No.2

Background: Although using fine needle cytology with aspiration (FNC-A) for establishing diagnoses in the retroperitoneal region has shown promise, there is scant literature supporting a role of non-aspiration cytology (FNC-NA) for this region. We assessed the accuracy and reliability of FNC-A and FNC-NA as tools for preoperative diagnosis of retroperitoneal masses and compared the results of both techniques with each other and with histopathology. Methods: Fifty-seven patients with retroperitoneal masses were subjected to FNC-A and FNC-NA. Smears were stained with May-Grunwald Giemsa and hematoxylin and eosin stain. An individual slide was objectively analysed using a point scoring system to enable comparison between FNC-A and FNC-NA. Results: By FNC-A, 91.7% accuracy was obtained in cases of retroperitoneal lymph node lesions followed by renal masses (83.3%). The diagnostic accuracy of other sites by FNC-A varied from 75.0%–81.9%. By FNC-NA, 93.4% diagnostically accurate results were obtained in the kidney, followed by 75.0% in adrenal masses. The diagnostic accuracy of other sites by FNC-NA varied from 66.7%–72.8%. Conclusions: Although both techniques have their own advantages and disadvantages, FNC-NA may be a more efficient adjuvant method of sampling in retroperitoneal lesions.

Optimization of Solid State Fermentation of Mustard (Brassica campestris) Straw for Production of Animal Feed by White Rot Fungi (Ganoderma lucidum)

Misra, A.K.,Mishra, A.S.,Tripathi, M.K.,Prasad, R.,Vaithiyanathan, S.,Jakhmola, R.C. Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.2

The objective of the experiment was to determine the optimum cultural [moisture levels (55, 60 and 70%), days of fermentation (7, 14 and 21), temperature (25 and $35^{\circ}C$) of incubation)] and nutritional parameters (urea addition (0 and 2%) and variable levels of single super phosphate (0.25 and 0.50% SSP)) for bio-processing of the mustard (Brassica campestris) straw (MS) under solid-state fermentation (SSF) system. The performance of SSF was assessed in terms of favorable changes in cell wall constituents, protein content and in vitro DM digestibility of the MS. Sorghum based inoculum (seed culture) of Ganoderma lucidum to treat the MS was prepared. The 50 g DM of MS taken in autoclavable polypropylene bags was mixed with a pre-calculated amount of water and the particular nutrient in the straw to attained the desired levels of water and nutrient concentration in the substrate. A significant progressive increase in biodegradation of DM (p<0.001), NDF (p<0.01) and ADF (p<0.05) was observed with increasing levels of moisture. Among the cell wall constituents the loss of ADF fraction was greatest compared to that of NDF. The loss of DM increased progressively as the fermentation proceeded and maximum DM losses occurred at 28 days after incubation. The protein content of the treated MS samples increased linearly up to the day $21^{th}$ of the incubation and thereafter declined at day $28^{th}$, whereas the improvement in in vitro DM digestibility were apparent only up to the day $14^{th}$ of the incubation under SSF and there after it declined. The acid detergent lignin (ADL) degradation was slower during the first 7 days of SSF and thereafter increased progressively and maximum ADL losses were observed at the day $28^{th}$ of the SSF. The biodegradation of DM and ADL was not affected by the variation in incubation temperature. Addition of urea was found to have inhibitory effect on fungal growth. The effect of both the levels (0.25 and 0.50) of SSP addition in the substrate, on DM, NDF, ADF, cellulose and ADL biodegradation was similar. Similarly, the protein content and the in vitro DM digestibility remain unaffected affected due to variable levels of the SSP inclusion in the substrate. From the results it may be concluded that the incubation of MS with 60 percent moisture for 21 days at $35^{\circ}C$ with 0.25 percent SSP was most suitable for MS treatment with Ganoderma lucidum. Maximum delignification, enrichment in the protein content and improvement in in vitro DM digestibility were achieved by adopting this protocol of bioprocessing of MS.

Effect of Extrusion Processing and Steam Pelleting Diets on Pellet Durability, Water Absorption and Physical Response of Macrobrachium rosenbergii

Misra, Chandra Kanta,Sahu, N.P.,Jain, K.K. Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.9

Two hundred and ten post-larvae (PL) of Macrobrachium rosenbergii (14.5-14.9 mg) were equally distributed in two experimental groups and fed with either steam cooked or extruded pellet for a period of 60 days. Physical evaluation and growth promoting effect of both the pellets were assessed. Significantly higher (p<0.05) water stability, absorption and protein efficiency ratio (PER) were recorded in extruded pellet than the steam cooked pellet. Nutrient loss was minimum in the extruded pellet for which lower feed conversion ratio (FCR) (p<0.05) was recorded in this group. However, weight gain, relative growth and specific growth rate (SGR) in both the groups were not significantly different (p>0.05). Although insignificant (p>0.05) but higher amylase activity was recorded in steam pellet fed group. Survival was not affected by feeding either of the diets.

Single Life Time Cytological Screening in High Risk Women as an Economical and Feasible Approach to Control Cervical Cancer in Developing Countries Like India

Misra, Jata Shankar,Srivastava, Anand Narain,Das, Vinita Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.3

In view of funding crunches and inadequate manpower in cytology in developing countries like India, single lifetime screening for cervical cancer has been suggested. In this study, an attempt was made to cscreening to make it more effective for early detection. Cytological data were derived from the ongoing routine cervical cytology screening program for women attending Gynaecology Out Patient Department of Queen Mary's Hospital of K.G.Medical University, Lucknow, India during a span of 35 years (April 1971 - December 2005). Cervical smears in a total of 38,256 women were cytologically evaluated. The frequencies of squamous intraepithelial lesions of cervix (SIL) and carcinoma cervix were found to be 7.0% and 0.6%, respectively, in the series. Predisposing factors related to cervical carcinogenesis were analyzed in detail to establish the most vulnerable groups of women for single life time screening. The incidence of SIL and carcinoma cervix was found to be maximal in women above the age of 40 years irrespective of parity and in multiparous women (with three or more children) irrespective of age. The incidence of cervical cytopathologies was significantly higher in symptomatic women, the frequency of SIL being alarmingly higher in women complaining of contact bleeding and that of carcinoma cervix in older women with postmenopausal bleeding. It is consequently felt that single life time screening must include the three groups of women delineated above. Such selective screening appears to be the most economical, cost effective and feasible approach to affordably control the menace of cervical cancer in developing countries like India.

A 32 nm NPN SOI HBT with Programmable Power Gain and 839 GHzV f_tBV_CEO Product

Misra, Prasanna Kumar,Qureshi, S. The Institute of Electronics and Information Engin 2014 Journal of semiconductor technology and science Vol.14 No.6

The performance of npn SiGe HBT on thin film SOI is investigated at 32 nm technology node by applying body bias. An n-well is created underneath thin BOX to isolate the body biased SOI HBT from SOI CMOS. The results show that the HBT voltage gain and power gain can be programmed by applying body bias to the n-well. This HBT can be used in variable gain amplifiers that are widely used in the receiver chain of RF systems. The HBT is compatible with 32 nm FDSOI technology having 10 nm film thickness and 30 nm BOX thickness. As the breakdown voltage increases by applying the body bias, the SOI HBT with 3 V $V_{CE}$ has very high $f_tBV_{CEO}$ product (839 GHzV). The self heating performance of the proposed SOI HBT is studied. The high voltage gain and power gain (60 dB) of this HBT will be useful in designing analog/RF systems which cannot be achieved using 32 nm SOI CMOS (usually voltage gain is in the range of 10-20 dB).

Applications of cold plasma technology for microbiological safety in meat industry

Misra, N.N.,Jo, C. Elsevier Trends Journals 2017 Trends in food science & technology Vol.64 No.-

<P>Key findings and conclusions: Majority of the studies have demonstrated the successful application of cold plasma for decontamination of meat and meat products. With rapid on-going developments in plasma science, plasma technologies could be used to replace conventional decontamination technologies in meat industry in the near future. (C) 2017 Elsevier Ltd. All rights reserved.</P>