Improvement in Clonal Propagation of Hemidesmus indicus R. Br. through Adenine Sulphate

Misra Neeta,Misra Pratibha,Datta S.K.,Mehrotra Shanta The Korean Society of Plant Biotechnology 2003 Plant molecular biology and biotechnology research Vol.5 No.4

A protocol has been developed for rapid large scale clonal propagation of an aromatic endangered medicinal plant, Hemidesmus indicus R. Br. with the elimination of the problems such as premature leaf fall and callus formation during caulogenesis and rhizogenesis. Multiple shoots were induced from shoot tip and nodal explants on Murashige and Skoog (MS) medium supplemented with 1 mg/L Benzylaminopurine (BAP) and 0.5 mg/L Napthaleneaceticacid (NAA). Addition of 15 mg/L adenine sulphate to the above medium checked leaf abscission completely, reduced the time required for caulogenesis and restored morphogenetic potential after several subcultures. The in vitro grown propagules were rooted in 1/2 MS medium supplemented with 2 mg/L Indolebutyric acid (IBA) +1 mg/L NAA and sucrose 0.7% (w/v). Addition of charcoal at 100 mg/L to the rooting medium quickened root initiation with a complete check on callus formation. The effect of sucrose concentration on both caulogenesis and rhizogenesis was also studied. The resultant plantlets were acclimatized and grown in fields where ninety eight percent of the rooted shoots survived and grew normally. The estimation of the secondary metabolite content in the shoots of the regenerated plant and the mother plant indicated that the concentration of the three secondary metabolites lupeol, vanillin and rutin was similar.

Some Desmids from Garhwal Region of Uttarakhand, India

Misra, Pradeep Kumar,Misra, Purnima,Shukla, Madhulika,Prakash, Jai The Korean Society of Phycology 2008 ALGAE Vol.23 No.3

The present paper consists of 42 taxa belonging to 7 genera of desmids (green algae) collected from two districts of Garhwal region of Uttarakhand (Western Himalayas). The district Haridwar is located 29° 55’to 29° 59’N latitude and 68° 5’to 68° 30’E longitude covering about 2360 km2 area and Dehradun district is situated between 77° 34’to 78° 18’E longitude and 29° 58’to 30° 58’N latitude. Seven genera of desmids are (with number of taxa in parenthesis): Closterium Nitzsch. (9), Cosmarium Corda ex Ralfs (25), Euastrum Ehr. (2), Spondylosium Breb. (1), Micrasterias Ag. (1), Staurastrum Meyen (3), Arthrodesmus Ehr. (1). All these taxa constitute new records for the area. The algal localities are relatively cleaner than those of majority of urban areas. A rich assemblage of desmids shows that water bodies of these hilly areas are still undisturbed and need protection for preservation of algal biodiversity.

Image-Guided Fine Needle Cytology with Aspiration Versus Non-Aspiration in Retroperitoneal Masses: Is Aspiration Necessary?

Rajiv Kumar Misra,Shaila Mitra,Rishav Kumar Jain,Shilpa Vahikar,Archana Bundela,Purak Misra 대한병리학회 2015 Journal of Pathology and Translational Medicine Vol.49 No.2

Background: Although using fine needle cytology with aspiration (FNC-A) for establishing diagnoses in the retroperitoneal region has shown promise, there is scant literature supporting a role of non-aspiration cytology (FNC-NA) for this region. We assessed the accuracy and reliability of FNC-A and FNC-NA as tools for preoperative diagnosis of retroperitoneal masses and compared the results of both techniques with each other and with histopathology. Methods: Fifty-seven patients with retroperitoneal masses were subjected to FNC-A and FNC-NA. Smears were stained with May-Grunwald Giemsa and hematoxylin and eosin stain. An individual slide was objectively analysed using a point scoring system to enable comparison between FNC-A and FNC-NA. Results: By FNC-A, 91.7% accuracy was obtained in cases of retroperitoneal lymph node lesions followed by renal masses (83.3%). The diagnostic accuracy of other sites by FNC-A varied from 75.0%–81.9%. By FNC-NA, 93.4% diagnostically accurate results were obtained in the kidney, followed by 75.0% in adrenal masses. The diagnostic accuracy of other sites by FNC-NA varied from 66.7%–72.8%. Conclusions: Although both techniques have their own advantages and disadvantages, FNC-NA may be a more efficient adjuvant method of sampling in retroperitoneal lesions.

Some Desmids from Garhwal Region of Uttarakhand, India

Pradeep Kumar Misra,Madhulika Shukla and Jai Prakash,Purnima Misra 한국조류학회I 2008 ALGAE Vol.23 No.3

The present paper consists of 42 taxa belonging to 7 genera of desmids (green algae) collected from two districts of Garhwal region of Uttarakhand (Western Himalayas). The district Haridwar is located 29° 55’ to 29° 59’ N latitude and 68° 5’ to 68° 30’ E longitude covering about 2360 km2 area and Dehradun district is situated between 77°34’ to 78° 18’ E longitude and 29° 58’ to 30° 58’ N latitude. Seven genera of desmids are (with number of taxa in parenthesis): Closterium Nitzsch. (9), Cosmarium Corda ex Ralfs (25), Euastrum Ehr. (2), Spondylosium Breb. (1), Micrasterias Ag. (1), Staurastrum Meyen (3), Arthrodesmus Ehr. (1). All these taxa constitute new records for the area. The algal localities are relatively cleaner than those of majority of urban areas. A rich assemblage of desmids shows that water bodies of these hilly areas are still undisturbed and need protection for preservation of algal biodiversity.

Helicobacter pylori Infection and a P53 Codon 72 Single Nucleotide Polymorphism: a Reason for an Unexplained Asian Enigma

Pandey, Renu,Misra, Vatsala,Misra, Sri Prakash,Dwivedi, Manisha,Misra, Alok Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21

Aim: P53, the most commonly mutated tumor suppressor gene in all types of human cancer, is involved in cell cycle arrest and control of apoptosis. Although p53 contains several polymorphic sites, the codon 72 polymorphism is by far more common. There are divergent reports but many studies suggest p53 pro/pro SNP may be associated with susceptibility to developing various cancers in different regions of the world. The present study aimed to find any correlation between H. pylori infection and progression of carcinogenesis, by studying apoptosis and the p53 gene in gastric biopsies from north Indian population. Materials and Methods: A total of 921 biopsies were collected and tested for prevalence of H. pylori by rapid urease test (RUT), imprint cytology and histology. Apoptosis was studied by the TUNEL method. Analysis of p53 gene polymorphism at codon 72 was accomplished by PCR using restriction enzyme BstU1. Observation: Out of 921 samples tested 56.7% (543) were H. pylori positive by the three techniques. The mean apoptotic index (AI) in the normal group was 2.12, while gastritis had the maximum 4.24 followed by gastric ulcer 2.28, gastropathy 2.22 and duodenal ulcer 2.08. Mean AI in cases with gastric cancer (1.72) was less than the normal group. The analysis of p53 72 SNP revealed that p53 (Arg/Arg), (Pro /Arg) variant are higher (40.59% & 33.66%) as compared to p53 pro/pro variant (25.74%) inthe healthy population. Conclusions: The North Indian population harbors Arg or Pro/Arg SNP that is capable of withstanding stress conditions; this may be the reason of low incidence of gastric disease in spite of high infection with H. pylori. There was no significant association with H. pylori infection and AI. However, there is increased apoptosis in gastritis which may occur independent of H. pylori or p53 polymorphism.

Atmospheric pressure cold plasma improves viscosifying and emulsion stabilizing properties of xanthan gum

Misra, N.N.,Yong, Hae In,Phalak, Rohit,Jo, Cheorun Elsevier 2018 Food hydrocolloids Vol.82 No.-

<P><B>Abstract</B></P> <P>In this work, we report the potential application of cold plasma technology for tailoring the functional properties of hydrocolloids. On treating xanthan gum in solid phase with atmospheric air cold plasma, we observed an increase in the viscosifying ability at low shear rates, and emulsion stabilizing activity of the gum, without affecting the colour or the basic polysaccharide backbone. The observed changes have potential benefits for salad-dressing and instant dry soup formulations.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Effect of cold plasma on properties of xanthan gum is evaluated. </LI> <LI> Plasma treatment of xanthan powder increases low-shear rate viscosity. </LI> <LI> The resulting xanthan has improved emulsion stabilizing capacity. </LI> <LI> The polysaccharide structure remains intact after plasma treatment. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Eco-friendly method of fabricating indium-tin-oxide thin films using pure aqueous sol-gel

Misra, Mrinmoy,Hwang, Deuk-Kyu,Kim, Yoon Cheol,Myoung, Jae-Min,Lee, Tae Il Elsevier 2018 CERAMICS INTERNATIONAL Vol.44 No.3

<P><B>Abstract</B></P> <P>An indium-tin-oxide (ITO) thin film with approximately 50 nm thickness was successfully synthesized on glass substrates by using a fully aqueous sol-gel process. The sol was prepared from indium nitrate hydrate and tin fluoride as a precursor. Thermogravimetric analysis confirmed that the sol converted into crystalline ITO at 286 °C. The optical band gap and transmittance of the thin film were observed to increase with annealing temperature and plasma treatment time. X-ray photoelectron spectroscopy and transmittance studies established that the number of oxygen vacancies in the thin film drastically increased with increasing temperature and plasma treatment. The annealing temperature and argon plasma treatment time appear to be key factors in reducing resistivity and increasing the transmittance of the thin film. A considerable decrease in the resistivity of the ITO thin film was observed after Ar plasma treatment. This eco-friendly sol-gel ITO thin film may find potential applications in n-type ohmic electrodes for ink-jet printable electronics.</P>

Components of Nutraceutical Value in Physalis minima

Misra, L.N.,Lal, P.,Kumar, D. The Korean Society of Food Science and Nutrition 2006 Preventive Nutrition and Food Science Vol.11 No.1

It is of utmost importance to feed the current world population by improving agricultural production with newer varieties of food crops, but what is still more important is to add nutrition into the food. Some of the plants, which are currently growing in the wastelands, contain certain phytochemicals which add to their neutraceutical and health value. These plants contain secondary metabolites which enhance the over all metabolic functions of the body. Withasteroids are one of such phytochemicals. These chemicals are almost exclusively found in plants of the Solanaceae family; one of which, Physalis minima, contains several with asteroids. The aerial parts and roots of P. minima have been found to contain several steroids, identification of which is been discussed in this paper. These with asteroids contribute to the functional value since incorporation of withanolides in the diet may prevent or decrease the growth of tumors in humans.

A 32 nm NPN SOI HBT with Programmable Power Gain and 839 GHzV f_tBV_CEO Product

Misra, Prasanna Kumar,Qureshi, S. The Institute of Electronics and Information Engin 2014 Journal of semiconductor technology and science Vol.14 No.6

The performance of npn SiGe HBT on thin film SOI is investigated at 32 nm technology node by applying body bias. An n-well is created underneath thin BOX to isolate the body biased SOI HBT from SOI CMOS. The results show that the HBT voltage gain and power gain can be programmed by applying body bias to the n-well. This HBT can be used in variable gain amplifiers that are widely used in the receiver chain of RF systems. The HBT is compatible with 32 nm FDSOI technology having 10 nm film thickness and 30 nm BOX thickness. As the breakdown voltage increases by applying the body bias, the SOI HBT with 3 V $V_{CE}$ has very high $f_tBV_{CEO}$ product (839 GHzV). The self heating performance of the proposed SOI HBT is studied. The high voltage gain and power gain (60 dB) of this HBT will be useful in designing analog/RF systems which cannot be achieved using 32 nm SOI CMOS (usually voltage gain is in the range of 10-20 dB).

High yield strength bulk Ti based bimodal ultrafine eutectic composites with enhanced plasticity

Misra, D. K.,Rakshit, R. K.,Singh, M.,Shukla, P. K.,Chaturvedi, K. M.,Sivaiah, B.,Gahtori, B.,Dhar, A.,Sohn, S. W.,Kim, W. T.,Kim, D. H. BUTTERWORTH - HEINEMANN 2014 MATERIALS AND DESIGN Vol.58 No.-

Ti-based bulk metallic glass (BMGs) and their bimodal composites are linked with the pronounced strain hardening after yielding but with much low value of strength. Therefore, developing Ti-based alloys with high yield strength and high plasticity is the current challenge. Here, we report the synthesis of ultra-fine grained bulk (UFG) (Ti0.705Fe0.295) 100 Ga-x(x) (0 <= x <= 2) bimodal eutectic composites with not only high strength and larger plasticity but also with high yield strength which is one of the important mechanical property for structural application. Reasonably high strength, high yield strength, strain to failure ratio, and enhanced plasticity of similar to 7 +/- 0.8% was observed in (Ti70.5Fe29.5)(98)Ga-2 composite which is superior than Ti-based BMGs and bimodal composites. Modification of degree of eutectic structure refinement and volume fraction of constituent phases with the addition of Ga are the crucial factors in enhancing the mechanical properties of Ti-Fi-(Ga) composites. (C) 2014 Elsevier Ltd. All rights reserved.