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RISS 인기검색어
- 검색키워드
- 저자 : Minghai Wei (검색결과 3 건)
- 무료
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Differentiation potential of neural stem cells derived from fetal sheep
Qian Li,Minghai Zhang,Wei Jun Guan,Shuang Zhang,Yanjie Zheng,Hebao Wen,Xiao Han 한국통합생물학회 2017 Animal cells and systems Vol.21 No.4
Neural stem cells (NSCs) are multipotent stem cells that can differentiate into many cell types in vitro. In this study, we isolated and established an NSC line from fetal Ovis aries. Based on the results of immunofluorescence staining, NSCs expressed Nestin, Pax6 and MAP2. Moreover, a reverse transcription–polymerase chain reaction assay was used to biologically characterize the cell line. NSCs were induced to differentiate into neurogenic cells in vitro. They expressed MAP2, glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP). In this study, we successfully isolated and cultivated NSCs from the hippocampal tissue of fetal sheep. NSCs not only displayed a self-renewal capacity but also had the potential to differentiate into neurons and glial cells. This study provided valuable experimental data for NSC transplant research.
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Plate gap effect on vicosity and rheological model of shear thickening fluid
Hou Shiwei,Lai Zhanwen,Wei Minghai 한국유변학회 2023 Korea-Australia rheology journal Vol.35 No.1
The present work investigates the eff ect of plate gap on the rheological properties of shear thickening fl uid (STF) and proposes a phenomenological model to predict the viscosity curve of STF for diff erent values of plate gap and temperature. Multiwalled carbon nanotube (MWCNT) reinforced silica-based STF (MWCNT/SiO 2 -STF) containing 0.8 wt% MWCNT and 20 wt% SiO 2 nanoparticles was prepared using polyethylene glycol as a dispersion medium and tested for its steady and dynamic rheological behavior at diff erent plate gaps. The peak viscosity of MWCNT/SiO 2 -STF follows the characteristic behavior of an initial increase followed by a subsequent decrease corresponding to the increase in plate gap. A maximum viscosity of 198.89 Pa s was recorded at a plate gap of 1.0 mm. Although signifi cant shear thinning in the dynamic rheological response of MWCNT/SiO 2 -STF was noticed at a 1.0 mm gap, the storage and loss modulus were better than those at 0.5 mm gap. The proposed model based predicts the shear thinning and thickening behavior of STF at low and high shear rates for diff erent values of plate gap with reasonable accuracy. The model also provides a very good fi t for the viscosity of STF at diff erent temperatures. Thus, the proposed model is suitable for numerical simulations as well as theoretical analysis in the vibration control field.
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Effect of bond slip on the performance of FRP reinforced concrete columns under eccentric loading
Chunyang Zhu,Li Sun,Ke Wang,Yue Yuan,Minghai Wei 사단법인 한국계산역학회 2019 Computers and Concrete, An International Journal Vol.24 No.1
Concrete reinforced with fiber reinforced polymer (FRP) bars (FRP-RC) has attracted a significant amount of research attention in the last three decades. Alimited number of studies, however, have investigated the effect of bond slip on the performance of FRP-RC columns under eccentric loading. Based on previous experimental study, a finite-element model of eccentrically loaded FRP-RC columns was established in this study. The bondslip behavior was modeled by inserting spring elements between FRP bars and concrete. The improved Bertero-Popov-Eligehausen (BPE) bond slip model with the results of existing FRP-RC pullout tests was introduced. The effect of bond slip on the entire compression-bending process of FRP-RC columns was investigated parametrically. The results show that the initial stiffness of bond slip is the most sensitive parameter affecting the compression-bending performance of columns. The peak bond stress and the corresponding peak slip produce a small effect on the maximum loading capacity of columns. The bondslip softening has little effect on the compression-bending performance of columns. The sectional analysis revealed that, as the load eccentricity and the FRP bar diameter increase, the reducing effect of bond slip on the flexural capacity becomes more obvious. With regard to bond slip, the axial-force-bendingmoment (P-M) interaction diagrams of columns with different FRP bar diameters show consistent trends. It can be concluded from this study that for columns reinforced with large diameter FRP bars, the flexural capacity of columns at low axial load levels will be seriously overestimated if the bond slip is not considered.
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