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Ming-feng Jiang,Sheng-wei Li,Min Chen,Ying-fan Cai,Yong-fang Xie,Biao Li,Quan Sun,Huai-zhong Jiang,Zheng Pan,Yun-ling Gao,You-Lu Yuan,Yu-zheng Shi 한국식물학회 2009 Journal of Plant Biology Vol.52 No.5
A cDNA encoding a novel cysteine proteinase inhibitor (CPI) was isolated from a gland mutant Xiangmian-18 of upland cotton during the pigments gland forming stage. The cDNA comprises 378 bp and encodes 125 amino acid residues with molecular mass of 13.8 kDa. It contains the conserved motif of cysteine protease inhibitors and belongs to the cystatin superfamily (Gln- Val-Val-Ala-Gly). The deduced amino acid sequences of the domains are highly similar to the normal upland cotton (96.8%). SDS-PAGE and western hybridization analysis showed that the expressed recombinant protein was recombinant CPI. The inhibitory activity of recombinant CPI was 46 u/μg which was measured by inhibiting the protease activity of papain. RT-PCR results indicated that the expression level of developing gland stage was higher than that of undeveloped gland stage.
Development of High Spectral Resolution Lidar System for Measuring Aerosol and Cloud
Ming Zhao,Chen-Bo Xie,Zhiqing Zhong,Bang-Xin Wang,Zhenzhu Wang,Pang-Da Dai,Zhen Shang,Min Tan,Dong Liu,Yingjian Wang 한국광학회 2015 Current Optics and Photonics Vol.19 No.6
A high spectral resolution lidar (HSRL) system based on injection-seeded Nd:YAG laser and iodineabsorption filter has been developed for the quantitative measurement of aerosol and cloud. The laserfrequency is stabilized at 80 MHz by a frequency locking system and the absorption line of iodine cellis selected at the 1111 line with 2 GHz width. The observations show that the HSRL can provide verticalprofiles of particle extinction coefficient, backscattering coefficient and lidar ratio for cloud and aerosolup to 12 km altitude, simultaneously. For the measured cases, the lidar ratios are 10~20 sr for cloud,28~37 sr for dust, and 58~70 sr for urban pollution aerosol. It reveals the potential of HSRL to distinguishthe type of aerosol and cloud. Time series measurements are given and demonstrate that the HSRL hasability to continuously observe the aerosol and cloud for day and night
Price Learning Based Load Distribution Strategies for Demand Response Management in Smart Grid
Qiang Tang,Ming-zhong Xie,Kun Yang,Yuan-sheng Luo,Ping Li 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.11
In this paper, a Price learning based Load Distribution Strategy (PLDS) is proposed at first. In PLDS model, Smart Power Service, Utility Company and History Load Curves are included, and by considering both the average electricity consumption cost and the average electricity consumption habit, we proposed a convex optimization model to solve the model. In order to accelerate the convergence of PLDS, a price learning mechanism is proposed, which learns a price curve according to the history price data, and predicts price as a learned price for the next iteration. The optimization cycle of PLDS is one day or 24 hours, and in order to further improve the peak shaving performance, an extended version of PLDS named PLRS (Price learning based Load Redistribution Strategy) is proposed, whose optimization cycle length is 1 hour. The optimization models of PLDS and PLRS are the same, and the differences between them are the optimization cycle and the constraint conditions. In the simulation, we compared the convergence performance, peaking shaving performance and total cost among PLDS, PLRS and other strategy ODC in reference [11], and we found that the convergence performances of PLDS and PLRS are both better than that of ODC. The peak shaving performance of PLRS is better than that of ODC in the long term, and the total cost of PLRS is very close to that of ODC.
Orthogonal 방법을 통한 Poly(AM-DMDAAC)/MMT 고흡수성나노복합체 제조 연구
Jun Dong Yuan,Ming Zhou,Shuang Qiao Yang,Yong Guo Zhou,Nan Qin,Song Tao He,Dong Lai,Zhong Qiang Xie 한국고분자학회 2014 폴리머 Vol.38 No.1
A novel poly(AM-DMDAAC)/MMT superabsorbent nanocomposites are prepared by radical polymerizationusing ammonium persulfate (APS) and anhydrous sodium sulfite as a free radical initiator and N,N-methylene bisacrylamide(MBA) as a crosslinker. In this paper, an optimization study on the synthesis of superabsorbent nanocompositesis carried out. Orthogonal array experiment indicates that the optimized conditions is acrylamide (AM) content 23 wt%,diallyl dimethyl ammonium chloride (DMDAAAC) content 6 wt%, montmorillonite (MMT) content 4 wt%, initiatorcontent 0.2 wt% and crosslinker content 0.02 wt%. Under the optimization syntheses conditions concluded, the maximumwater absorbency in distilled water is 659.53 g·g-1 and in 2 wt% sodium chloride solution is 116.25 g·g-1. Compared withthe range values of different factors (Rj), the order of significance factors in distilled water is C (MMT) > B (DMDAAC)> A (AM) > D (crosslinker) > E (initiator). MMT is intercalated during polymerization reaction and a nanocompositestructure is formed as shown by TEM analysis and XRD analysis.
Control Over the Branched Structures of Platinum Nanocrystals for Electrocatalytic Applications
Ma, Liang,Wang, Chengming,Gong, Ming,Liao, Lingwen,Long, Ran,Wang, Jinguo,Wu, Di,Zhong, Wei,Kim, Moon J.,Chen, Yanxia,Xie, Yi,Xiong, Yujie American Chemical Society 2012 ACS NANO Vol.6 No.11
<P>Structural control of branched nanocrystals allows tuning two parameters that are critical to their catalytic activitythe surface-to-volume ratio, and the number of atomic steps, ledges, and kinks on surface. In this work, we have developed a simple synthetic system that allows tailoring the numbers of branches in Pt nanocrystals by tuning the concentration of additional HCl. In the synthesis, HCl plays triple functions in tuning branched structures <I>via</I> oxidative etching: (i) the crystallinity of seeds and nanocrystals; (ii) the number of {111} or {100} faces provided for growth sites; (iii) the supply kinetics of freshly formed Pt atoms in solution. As a result, tunable Pt branched structurestripods, tetrapods, hexapods, and octopods with identical chemical environmentcan be rationally synthesized in a single system by simply altering the etching strength. The controllability in branched structures enables to reveal that their electrocatalytic performance can be optimized by constructing complex structures. Among various branched structures, Pt octopods exhibit particularly high activity in formic acid oxidation as compared with their counterparts and commercial Pt/C catalysts. It is anticipated that this work will open a door to design more complex nanostructures and to achieve specific functions for various applications.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2012/ancac3.2012.6.issue-11/nn304237u/production/images/medium/nn-2012-04237u_0010.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn304237u'>ACS Electronic Supporting Info</A></P>
miR-200a Inhibits Tumor Proliferation by Targeting AP-2γ in Neuroblastoma Cells
Gao, Shun-Li,Wang, Li-Zhong,Liu, Hai-Ying,Liu, Dan-Li,Xie, Li-Ming,Zhang, Zhi-Wei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11
Background: MicroRNA-200a (miR-200a) has been reported to regulate tumour progression in several tumours but little is known about its role in neuroblastoma. Our aim was to investigate the potential role and mechanism of miR-200a in neuroblastomas. Materials and Methods: Expression levels of miR-200a in tissues were determined using RT-PCR. The effect of miR-200a and shAP-$2{\gamma}$ on cell viability was evaluated using MTS assays, and target protein expression was determined using Western blotting and RT-PCR. Luciferase reporter plasmids were constructed to confirm direct targeting. Results were reported as mean${\pm}$S.E.M and differences were tested for significance using the 2-tailed Students t-test. Results: We determined that miR-200a expression was significantly lower in neuroblastoma tumors than the adjacent non-cancer tissue. Over-expression of miR-200 are reduced cell viability in neuroblastoma cells and inhibited tumor growth in mouse xenografts. We identified AP-$2{\gamma}$ as a novel target for miR-200a in neuroblastoma cells. Thus miR-200a targets the 3'UTR of AP-$2{\gamma}$ and inhibits its mRNA and protein expression. Furthermore, our result showed that shRNA knockdown of AP-$2{\gamma}$ in neuroblastoma cells results in significant inhibit of cell proliferation and tumor growth in vitro, supporting an oncogenic role of AP-$2{\gamma}$ in neuroblastoma. Conclusions: Our study revealed that miR-200a is a candidate tumor suppressor in neuroblastoma, through direct targeting of AP-$2{\gamma}$. These findings re-enforce the proposal of AP-$2{\gamma}$ as a therapeutic target in neuroblastoma.
Profiling Gene Expression During Gland Morphogenesis of a Glanded and a Glandless Upland Cotton
Ying-Fan Cai,Min Chen,Quan Sun,Yong-Fang Xie,Sheng-Wei Li,Jian-Chuan Mo,Ming-Feng Jiang,You-Lu Yuan,Yu-Zhen Shi,Huai-Zhong Jiang,Zheng Pan,Yun-Ling Gao,Peng-Sheng Ye,Hua-Lan Zeng 한국식물학회 2009 Journal of Plant Biology Vol.52 No.6
The pigment gland is an important character of the Gossypium plant. With the aim of identifying genes involved in pigment gland morphogenesis in cotton, gene expression during pigment gland morphogenesis in Chuan 2802, which is glanded both in seed and plant, and a glandless line N5 was profiled using Affymetrix Cotton microarray. The results showed that there were 564 differentially expressed genes greater than twofold during gland morphogenesis. About 60.2% of these genes shares similarity with known genes on GenBank and about 39.8% with no functional description in the database. These described genes may play roles in defense response, response to oxidative stress, peroxidase activity, and the other metabolic pathways. The KEGG Orthology-Based Annotation System indicated that these above twofold expressed genes involved seven biochemical pathways on KEGG. These findings suggest that a complicated regulation is associated with pigment gland morphogenesis and the associated defense response including gossypol biosynthesis in cotton.
Song, Qing-Kun,Li, Jing,Huang, Rong,Fan, Jin-Hu,Zheng, Rong-Shou,Zhang, Bao-Ning,Zhang, Bin,Tang, Zhong-Hua,Xie, Xiao-Ming,Yang, Hong-Jian,He, Jian-Jun,Li, Hui,Li, Jia-Yuan,Qiao, You-Lin,Chen, Wan-Qin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22
Background: The study aimed to describe the age distribution of breast cancer diagnosis among Chinese females for comparison with the United States and the European Union, and provide evidence for the screening target population in China. Materials and Methods: Median age was estimated from hospital databases from 7 tertiary hospitals in China. Population-based data in China, United States and European Union was extracted from the National Central Cancer Registry, SEER program and GLOBOCAN 2008, respectively. Age-standardized distribution of breast cancer at diagnosis in the 3 areas was estimated based on the World Standard Population 2000. Results: The median age of breast cancer at diagnosis was around 50 in China, nearly 10 years earlier than United States and European Union. The diagnosis age in China did not vary between subgroups of calendar year, region and pathological characteristics. With adjustment for population structure, median age of breast cancer at diagnosis was 50~54 in China, but 55~59 in United States and European Union. Conclusions: The median diagnosis age of female breast cancer is much earlier in China than in the United States and the European Union pointing to racial differences in genetics and lifestyle. Screening programs should start at an earlier age for Chinese women and age disparities between Chinese and Western women warrant further studies.
Li, Chen-Long,Chang, Liang,Guo, Lin,Zhao, Dan,Liu, Hui-Bin,Wang, Qiu-Shi,Zhang, Ping,Du, Wen-Zhong,Liu, Xing,Zhang, Hai-Tao,Liu, Yang,Zhang, Yao,Xie, Jing-Hong,Ming, Jian-Guang,Cui, Yu-Qiong,Sun, Ying Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23
Background: ${\beta}$-elemene, extracted from herb medicine Curcuma wenyujin has potent anti-tumor effects in various cancer cell lines. However, the activity of ${\beta}$-elemene against glioma cells remains unclear. In the present study, we assessed effects of ${\beta}$-elemene on human glioma cells and explored the underlying mechanism. Materials and Methods: Human glioma U87 cells were used. Cell proliferation was determined with MTT assay and colony formation assay to detect the effect of ${\beta}$-elemene at different doses and times. Fluorescence microscopy was used to observe cell apoptosis with Hoechst 33258 staining and change of glioma apoptosis and cell cycling were analyzed by flow cytometry. Real-time quantitative PCR and Western-blotting assay were performed to investigated the influence of ${\beta}$-elemene on expression levels of Fas/FasL, caspase-3, Bcl-2 and Bax. The experiment was divided into two groups: the blank control group and ${\beta}$-elemne treatment group. Results: With increase in the concentration of ${\beta}$-elemene, cytotoxic effects were enhanced in the glioma cell line and the concentration of inhibited cell viability ($IC_{50}$) was $48.5{\mu}g/mL$ for 24h. ${\beta}$-elemene could induce cell cycle arrest in the G0/G1 phase. With Hoechst 33258 staining, apoptotic nuclear morphological changes were observed. Activation of caspase-3,-8 and -9 was increased and the pro-apoptotic factors Fas/FasL and Bax were upregulated, while the anti-apoptotic Bcl-2 was downregulated after treatment with ${\beta}$-elemene at both mRNA and protein levels. Furthermore, proliferation and colony formation by U87 cells were inhibited by ${\beta}$-elemene in a time and does-dependent manner. Conclusions: Our results indicate that ${\beta}$-elemene inhibits growth and induces apoptosis of human glioma cells in vitro. The induction of apoptosis appears to be related with the upregulation of Fas/FasL and Bax, activation of caspase-3,-8 and -9 and downregulation of Bcl-2, which then trigger major apoptotic cascades.