Experimental Study on Sustained-release 5-Fluorouracil Implantation in Canine Peritoneum and Para-aortic Abdominalis

Wei, Guo,Nie, Ming-Ming,Shen, Xiao-Jun,Xue, Xu-Chao,Ma, Li-Ye,Du, Cheng-Hui,Wang, Shi-Liang,Bi, Jian-Wei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

Objective: To observe local and systemic toxicity after sustained-release 5-fluorouracil (5-Fu) implantation in canine peritoneum and para-aortic abdominalis and the changes of drug concentration in the local implanted tissue with time. Methods: 300 mg sustained-release 5-Fu was implanted into canine peritoneum and para-aorta abdominalis. Samples were taken 3, 5, 7 and 10 days after implantation for assessment of changes and systemic reactions. High performance liquid chromatography was applied to detect the drug concentrations of peritoneal tissue at different distances from the implanted site, lymphatic tissue of para-aortic abdominalis, peripheral blood and portal venous blood. Results: 10 days after implantation, the drug concentrations in the peritoneum, lymphatic tissue and portal vein remained relatively high within 5 cm of the implanted site. There appeared inflammatory reaction in the local implanted tissue, but no visible pathological changes such as cell degeneration and necrosis, and systemic reaction like anorexia, nausea, vomiting and fever. Conclusions: Sustained-release 5-Fu implantation in canine peritoneum and para-aortic abdominalis can maintain a relatively high tumour-inhibiting concentration for a longer time in the local implanted area and portal vein, and has mild local and systemic reactions. Besides, it is safe and effective to prevent or treat recurrence of gastrointestinal tumours and liver metastasis.

Tumor-derived CD4+CD25+ Tregs Inhibit the Maturation and Antigen-Presenting Function of Dendritic Cells

Du, Yong,Chen, Xin,Lin, Xiu-Qing,Wu, Wei,Huang, Zhi-Ming Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.7

CD4+CD25+regulatory T cells (Tregs) play a key role in regulation of immnue response and maintenance of self-tolerance. Studies have found Tregs could suppress tumor-specific T cell-mediated immune response and promote cancer progression. Depletion of Tregs can enhance antitumor immunity. Dendritic cells (DCs) are professional antigen-presenting cells and capable of activating antigen-specific immune responses, which make them ideal candidate for cancer immunotherapy. Now various DC vaccines are considered as effective treatment for cancers. The aim of this study was to evaluate variation of Tregs in BALB/C mice with hepatocellular carcinoma and investigate the interaction between tumor-derived Tregs, effector T cells (Teff) and splenic DCs. We found the percentages of Tregs/CD4+ in the peripheral blood of tumor-bearing mice were higher than in normal mice. Tumor-derived Tregs diminished the up-regulation of costimulatory molecule expression on splenic DCs, even in the presence of Teff cells and simultaneously inhibited IL-12 and $TNF-{\alpha}$ secretion by DCs.

Evaluate VTS benefits: A case study of Zhoushan Port

Jun-Min MOU,Cui ZHOU,Yu DU,Wei-Ming TANG 국제이네비해양경제학회 2015 International Journal of e-Navigation and Maritime Vol.3 No.1

It has been well acknowledged that Vessel Traffic Services (VTS) has played a growing important role to ensure the safety of navigation in the busy ports and waterways. However, the benefits produced by VTS are usually ignored by the public and private sectors. Besides, the previous evaluations generally exist following problems: (1) It is difficult to collect the data for the parameters in the evaluation models and/or the parameters are designed illogically; (2) Those models did not take the following factors into consideration such as reducing the frequency of coastal vessel patrolling and saving human and material resources; (3) It is difficult to clearly discriminate the benefits derived from VTS and non-VTS. In this paper, a framework is presented to calculate the benefits of VTS in China. Four key indicators (safety, traffic efficiency, environmental protection and reducing supervising cost) and quantitative methods have been introduced into the framework. When calculating the benefits quantitatively, the traffic condition before the construction (expansion) of the VTS has acted as a benchmark. For a case study, the project of the expansion of VTS in Zhoushan Port, East China was evaluated with 10-year data. According to the results, the largest contribution is from the benefit of environmental protection. Via Cost-benefit analysis the benefit cost ratio (B/C) of the VTS is up to 5.248, which shows the benefits produced by VTS are considerable. The research could provide references for VTS benefits evaluation and investment optimizing.

SKF96365 impedes spinal glutamatergic transmission-mediated neuropathic allodynia

Qiru Wang,Yang Zhang,Qiong Du,Xinjie Zhao,Wei Wang,Qing Zhai,Ming Xiang 대한약리학회 2023 The Korean Journal of Physiology & Pharmacology Vol.27 No.1

Spinal nerve injury causes mechanical allodynia and structural imbalance of neurotransmission, which were typically associated with calcium overload. Storeoperated calcium entry (SOCE) is considered crucial elements-mediating intracellular calcium homeostasis, ion channel activity, and synaptic plasticity. However, the underlying mechanism of SOCE in mediating neuronal transmitter release and synaptic transmission remains ambiguous in neuropathic pain. Neuropathic rats were operated by spinal nerve ligations. Neurotransmissions were assessed by whole-cell recording in substantia gelatinosa. Immunofluorescence staining of STIM1 with neuronal and glial biomarkers in the spinal dorsal horn. The endoplasmic reticulum stress level was estimated from qRT-PCR. Intrathecal injection of SOCE antagonist SKF96365 dose-dependently alleviated mechanical allodynia in ipsilateral hind paws of neuropathic rats with ED50 of 18 μg. Immunofluorescence staining demonstrated that STIM1 was specifically and significantly expressed in neurons but not astrocytes and microglia in the spinal dorsal horn. Bath application of SKF96365 inhibited enhanced miniature excitatory postsynaptic currents in a dosage-dependent manner without affecting miniature inhibitory postsynaptic currents. Mal-adaption of SOCE was commonly related to endoplasmic reticulum (ER) stress in the central nervous system. SKF96365 markedly suppressed ER stress levels by alleviating mRNA expression of C/ EBP homologous protein and heat shock protein 70 in neuropathic rats. Our findings suggested that nerve injury might promote SOCE-mediated calcium levels, resulting in long-term imbalance of spinal synaptic transmission and behavioral sensitization, SKF96365 produces antinociception by alleviating glutamatergic transmission and ER stress. This work demonstrated the involvement of SOCE in neuropathic pain, implying that SOCE might be a potential target for pain management

Enhanced Tendon-to-Bone Healing of Chronic Rotator Cuff Tears by Bone Marrow Aspirate Concentrate in a Rabbit Model

Xiao Ning Liu,양철중,김지의,Zhen Wu Du,Ming Ren,Wei Zhang,Hong Yu Zhao,김경옥,노규철 대한정형외과학회 2018 Clinics in Orthopedic Surgery Vol.10 No.1

Background: To evaluate the influence of bone marrow aspirate concentrate (BMAC) on tendon-to-bone healing in a rabbit rotator cuff model and to characterize the composition of growth factors in BMAC. Methods: In this in vivo study, 40 rabbits were allocated into five groups: control (C), repair + saline (RS), repair + platelet-rich plasma (PRP; RP), repair + BMAC (RB) and repair + PRP + BMAC (RPB). A tear model was created by supraspinatus tendon transection at the footprint. Six weeks after transection, the torn tendon was repaired along with BMAC or PRP administration. Six weeks after repair, shoulder samples were harvested for biomechanical and histological testing. Ten rabbits were used for processing PRP and BMAC, followed by analysis of blood cell composition and the levels of growth factors in vitro . Results: The ultimate load-to-failure was significantly higher in RPB group compared to RS group (p = 0.025). BMAC-treated groups showed higher values of biomechanical properties than RS group. The histology of BMAC-treated samples showed better collagen fiber continuity and orientation than RS group. BMAC contained significantly higher levels of the several growth factors than PRP. Conclusions: Locally administered BMAC enhanced tendon-to-bone healing and has potential for clinical applications.

Leaf Wilting Movement Can Protect Water-Stressed Cotton (Gossypium hirsutum L.) Plants Against Photoinhibition of Photosynthesis and Maintain Carbon Assimilation in the Field

Ya-Li Zhang,Hong-Zhi Zhang,Ming-Wei Du,Wei Li,Hong-Hai Luo,Wah-Soon Chow,Wang-Feng Zhang 한국식물학회 2010 Journal of Plant Biology Vol.53 No.1

Under severe water stress, leaf wilting is quite general in higher plants. This passive movement can reduce the energy load on a leaf. This paper reports an experimental test of the hypothesis that leaf wilting movement has a protective function that mitigates against photoinhibition of photosynthesis in the field. The experiments exposed cotton (Gossypium hirsutum L.) to two water regimes: waterstressed and well-watered. Leaf wilting movement occurred in water-stressed plants as the water potential decreased to −4.1 MPa, reducing light interception but maintaining comparable quantum yields of photosystem II (PS II; Yield for short) and the proportion of total PS II centers that were open (qP). Predrawn Fv/Fm (potential quantum yield of PS II) as an indicator of overnight recovery of PS II from photoinhibition was higher than or similar to that in wellwatered plants. Compared with water-stressed cotton leaves for which wilting movement was permitted, water-stressed cotton leaves restrained from such movement had significantly increased leaf temperature and instantaneous CO2assimilation rates in the short term, but reduced Yield, qP,and Fv/Fm. In the long term, predrawn Fv/Fm and CO2assimilation capacity were reduced in water-stressed leaves restrained from wilting movement. These results suggest that, under water stress, leaf wilting movement could reduce the incident light on leaves and their heat load, alleviate damage to the photosynthetic apparatus due to photoinhibition,and maintain considerable carbon assimilation capacity in the long term despite a partial loss of instantaneous carbon assimilation in the short term.

Studies on the Ginseng Polypeptide - Decreasing Blood Sugar and Hapatic Glycogen

(Jin Zhang),(Hong Ying Zhang),(Wen Yun Du),(Da Wei Wang),(Ben Xiang Wang),(Ming Yang),(Yu Lian Jin),(Zhi Yong Cui),(Yan Wang) 고려인삼학회 1990 Proceedings of International Symposium on Korean G Vol.- No.-

Phenolic constituents from Parakmeria yunnanensis and their anti-HIV-1 activity

Shan-Zhai Shang,Huan Chen,Cheng-Qin Liang,Zhong-Hua Gao,Xue Du,Rui-Rui Wang,Yi-Ming Shi,Yong-Tang Zheng,Wei-Lie Xiao,Han Dong Sun 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.10

Three new phenolic compounds, yunnanensinsA–C (1–3), together with fourteen known ones (4–17),were isolated from the leaves and stems of Parakmeriayunnanensis. The structures of new compounds wereestablished on the basis of extensive spectroscopic analyses. Several compounds showed weak anti-HIV-1 activity.

Isocoumarin Derivatives from the Sea Squirt-derived Fungus Penicillium stoloniferum QY2-10 and the Halotolerant Fungus Penicillium notatum B-52

Xin, Zhi-Hong,Tian, Li,Zhu, Tian-Jiao,Wang, Wen-Liang,Du, Lin,Fang, Yu-Chun,Gu, Qian-Qun,Zhu, Wei-Ming 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.7

Two isocoumarin derivatives, stoloniferol A (1) and B (2), a known $5{\alpha}$, $8{\alpha}-epidioxy-23-methyl-(22E, 24R)-ergosta-6$, $22-dien-3{\beta}-ol$ (3), and a known dihydrocitrinone (4) were isolated from the ethyl acetate extract of the sea squirt-derived fungus, Penicillium stoloniferum QY2-10, and a halophilic fungus, Penicillium notatum B-52, respectively. Their structures were elucidated by spectroscopic methods and optical rotation. The stereochemistry of 2 was determined on the basis of different NOE experiments and chemical transformation. Compound 3 showed cytotoxicity against P388 cells, with an $IC_{50}$ value of 4.07 ${\mu}M$.

Strategies of Functional Food for Cancer Prevention in Human Beings

Zeng, Ya-Wen,Yang, Jia-Zheng,Pu, Xiao-Ying,Du, Juan,Yang, Tao,Yang, Shu-Ming,Zhu, Wei-Hua Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

Functional food for prevention of chronic diseases is one of this century's key global challenges. Cancer is not only the first or second leading cause of death in China and other countries across the world, but also has diet as one of the most important modifiable risk factors. Major dietary factors now known to promote cancer development are polished grain foods and low intake of fresh vegetables, with general importance for an unhealthy lifestyle and obesity. The strategies of cancer prevention in human being are increased consumption of functional foods like whole grains (brown rice, barley, and buckwheat) and by-products, as well some vegetables (bitter melon, garlic, onions, broccoli, and cabbage) and mushrooms (boletes and Tricholoma matsutake). In addition some beverages (green tea and coffee) may be protective. Southwest China (especially Yunnan Province) is a geographical area where functional crop production is closely related to the origins of human evolution with implications for anticancer influence.