Preventive Effects of Colon Colitis in Balb/c Mice by Doenjang Prepared with Starters and Bamboo Salts

Fang-Fang Liu,Min-kyeng Choi,Min-Woo Jeong,Kun-Young Park 한국식품영양과학회 2013 한국식품영양과학회 학술대회발표집 Vol.2013 No.11

Studies on Development of Preparation Method of Black Soybean Chungkukjang

Fang-Fang Liu,Min-kyeng Choi,Min-Woo Jeong,Kun-Young Park 한국식품영양과학회 2013 한국식품영양과학회 학술대회발표집 Vol.2013 No.11

Numerical investigations on the along-wind response of a vibrating fence under wind action

Fang, Fuh-Min,Ueng, Jin-Min,Chen, J.C. Techno-Press 2002 Wind and Structures, An International Journal (WAS Vol.5 No.2

The along-wind response of a surface-mounted elastic fence under the action of wind was investigated numerically. In the computations, two sets of equations, one for the simulation of the unsteady turbulent flow and the other for the calculation of the dynamic motion of the fence, were solved alternatively. The resulting time-series tip response of the fence as well as the flow fields were analyzed to examine the dynamic behaviors of the two. Results show that the flow is unsteady and is dominated by two frequencies: one relates to the shear layer vortices and the other one is subject to vortex shedding. The resulting unsteady wind load causes the fence to vibrate. The tip deflection of the fence is periodic and is symmetric to an equilibrium position, corresponding to the average load. Although the along-wind aerodynamic effect is not significant, the fluctuating quantities of the tip deflection, velocity and acceleration are enhanced as the fundamental frequency of the fence is near the vortex or shedding frequency of the flow due to the occurrence of resonance. In addition, when the fence is relatively soft, higher mode response can be excited, leading to significant increases of the variations of the tip velocity and acceleration.

High Pt loading on functionalized multiwall carbon nanotubes as a highly efficient cathode electrocatalyst for proton exchange membrane fuel cells

Fang, Baizeng,Kim, Min-Sik,Kim, Jung Ho,Song, Min Young,Wang, Yan-Jie,Wang, Haijiang,Wilkinson, David P.,Yu, Jong-Sung Royal Society of Chemistry 2011 Journal of materials chemistry Vol.21 No.22

<P>A very efficient, reproducible approach has been developed to fabricate a multiwall carbon nanotube (MWCNT)-supported, high Pt loading electrocatalyst. In this strategy, MWCNT was first functionalized with an anionic surfactant, sodium dodecylsulfate (SDS), to enhance the hydrophilicity of the MWCNTs for high Pt loading. The SDS-modified MWCNTs were further used to support high loading of Pt nanoparticles (NPs) through a urea-assisted homogeneous deposition (HD) strategy, followed by reduction using ethylene glycol (EG) as the precursor of a reducing agent. Through the input of SDS on the MWCNTs, Pt complex species can be readily anchored on the outer surface of the MWCNTs, while <I>in situ</I> pH adjustment of the solution with urea and reduction by EG enable the Pt NPs to disperse very uniformly on the SDS–MWCNT support with small particles size. Due to its unique structural characteristics, such as high electronic conductivity, the one-dimensional nanotube structure favouring fast electron transfer and more uniform Pt NP dispersion on the support with smaller particle size, the SDS-MWCNT-supported Pt (60 wt%) catalyst considerably outperformed a commercially available Johnson Matthey catalyst with the same Pt loading supported on Vulcan carbon black, when they were each employed as a cathode catalyst in proton exchange membrane fuel cells.</P> <P>Graphic Abstract</P><P>Non-covalent functionalization of MWCNT by SDS gives high density anchoring of Pt(<SMALL>IV</SMALL>) species, while an EG homogeneous reduction allows a small metal particle size, resulting in greatly improved PEMFC performance for SDS–MWCNT-supported Pt cathode catalysts. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1jm10847f'> </P>

Development of a Molecular Marker for Fruiting Body Pattern in Auricularia auricula-judae

( Fang-jie Yao ),( Li-xin Lu ),( Peng Wang ),( Ming Fang ),( You-min Zhang ),( Ying Chen ),( Wei-tong Zhang ),( Xiang-hui Kong ),( Jia Lu ),( Yoichi Honda ) 한국균학회 2018 Mycobiology Vol.46 No.1

The fruiting body pattern is an important agronomic trait of the edible fungus Auricularia auricula-judae, and an important breeding target. There are two types of fruiting body pattern: the cluster type and the chrysanthemum type. We identified the fruiting body pattern of 26 test strains, and then constructed two different near-isogenic pools. Then, we developed sequence characterized amplified region (SCAR) molecular markers associated with the fruiting body pattern based on sequence-related amplified polymorphism (SRAP) markers. Ten different bands (189-522 bp) were amplified using 153 pairs of SRAP primers. The SCAR marker “SCL-18” consisted of a single 522-bp band amplified from the cluster-type strains, but not the chrysanthemum strains. This SCAR marker was closely associated with the cluster- type fruiting body trait of A. auricula-judae. These results lay the foundation for further research to locate and clone genes controlling the fruiting body pattern of A. auricula-judae.

Knockdown of Chloride Channel-3 Inhibits Breast Cancer Growth In Vitro and In Vivo

Fang-Min Zhou,Yun-Ying Huang,Tian Tian,Xiao Yan Liu,Yong-Bo Tang 한국유방암학회 2018 Journal of breast cancer Vol.21 No.2

Purpose: Chloride channel-3 (ClC-3) is a member of the chloride channel family and plays a critical role in a variety of cellular activities. The aim of the present study is to explore the molecular mechanisms underlying the antitumor effect of silencing ClC-3 in breast cancer. Methods: Human breast cancer cell lines MDAMB- 231 and MCF-7 were used in the experiments. Messenger RNA and protein expression were examined by quantitative realtime polymerase chain reaction and western blot analysis. Cell proliferation was measured by the bromodeoxyuridine method, and the cell cycle was evaluated using fluorescence-activated cell sorting. Protein interaction in cells was analyzed by co-immunoprecipitation. Tumor tissues were stained with hematoxylin- eosin and tumor burden was measured using the Metamorph software. Results: Breast cancer tissues collected from patients showed an increase in ClC-3 expression. Knockdown of ClC-3 inhibited the secretion of insulin-like growth factor (IGF)-1, cell proliferation, and G1/S transition in breast cancer cells. In the mouse xenograft model of human breast carcinoma, tumor growth was significantly slower in animals injected with ClC- 3-deficient cells compared with the growth of normal human breast cancer cells. In addition, silencing of ClC-3 attenuated the expression of proliferating cell nuclear antigen, Ki-67, cyclin D1, and cyclin E, as well as the activation of extracellular signalregulated protein kinases (ERK) 1/2, both in vitro and in vivo. Conclusion: Together, our data suggest that upregulation of ClC- 3 by IGF-1 contributes to cell proliferation and tumor growth in breast cancer, and ClC-3 deficiency suppresses cell proliferation and tumor growth via the IGF/IGF receptor/ERK pathway.

Identification and expression analysis of ERECTA family genes in grape (Vitis vinifera L.)

Min Liu,Wanping Li,Zhuo Min,Xianghan Cheng,Yulin Fang 한국유전학회 2019 Genes & Genomics Vol.41 No.6

Background ERECTA family (ERf) genes are found in many dicots and monocots, and play important roles in plant developmental processes and stress responses. However, there is little known on ERf genes in grape (Vitis vinifera L.). Objectives The primary objective of this study was to identify the ERf genes in grape, and to analyze their expression profiles in different organs, during development, and in response to hormone treatments and abiotic/biotic stresses. Methods ERf protein sequences of dicots were aligned in the grape genome (V. vinifera cv. Pinot Noir, PN40024, 12X) with Blast server. The locus tags obtained were inputted in NCBI to retrieve corresponding nucleotide and protein accession numbers. The subcellular localization experiment was performed by the transient expression of VvERECTA-GFP and VvERL2-GFP in mesophyll protoplasts of Arabidopsis. The expression levels of ERf genes in grape leaves were detected by qRT-PCR after hormone treatments and abiotic/biotic stresses. Results We first identified the ERf genes in the grape genome, including VvERECTA and VvERL2. Their cDNA full-length sequences were obtained with the accession numbers MG601756 and MG601757. The result of subcellular localization indicated that the fusion proteins of VvERECTA and VvERL2 were localized in the plasma membrane. There were four conserved domains identified in VvERECTA and VvERL2, including a LRRNT-2, a LRR, a transmembrane and a protein kinase domain. The grape ERf genes expressed highly in young aboveground organs. As grape leaves or berries becoming mature, VvERECTA expressed in a declining trend. The transcript abundance of VvERL2 decreased during leaves development, but showed no significant differences during berries development. The hormone treatments of ABA, SA, MeJA and BR could induce the expression of VvERECTA and VvERL2. The treatments of heat, drought, downy and powdery mildew significantly increased the expression levels of the grape ERf genes. Conclusion The grape ERECTA gene family might play crucial roles in response to abiotic and biotic stresses. We provide the first description of the grape ERf genes and the most comprehensive analysis of their expressions in different biological processes.

RESEARCH : Effects of repeated tuberculin skin testings on immune responses in experimental monkeys

( Fang Ui Min ),( Jing Wang ),( Wen Yuan ),( Hui Wen Kuang ),( Wei Bo Zhao ) 한국동물자원과학회(구 한국축산학회) 2014 한국축산학회지 Vol.56 No.32

Though many alternative methods to tuberculin skin testing (TST) have been established and evaluated in recent years, sensitivities and specificities of most methods could not meet the requirements of golden standards. In this study, we sought to identify whether repeated TSTs could affect the immune responses in experimental monkeys. Nine natural tuberculosis (TB) monkeys receiving repeated TSTs biweekly were used to demonstrate the effect on TST responsiveness. Two healthy monkeys were administrated with repeated TSTs to analyze the immune response profiling. Intrapalpebral reactions in TB infections gradually weakened or presented intermittent positive reactions. The leukocyte counts, cytokine responses, and antibody responses to all antigens except Old tuberculin (OT) and MPT64L showed no specific changes for TB in healthy monkeys. Positive antibody responses to OT and MPT64L emerged during the first half experimental period, which may cause by their cross-reactivity with mycobacterial species. Results showed that repeated TSTs had no significant effects on immune responses in healthy monkeys but a progressive reduction in TST responsiveness in TB infections.

The acrosswind response of the downwind prism in a twin-prism system with a staggered arrangement

Fang, Fuh-Min,Chung, Cheng-Yang,Li, Yi-Chao,Liu, Wen-Chin,Lei, Perng-Kwei Techno-Press 2013 Wind and Structures, An International Journal (WAS Vol.17 No.3

The flow interaction between two identical neighboring twin square prisms in a staggered arrangement in an open terrain was investigated experimentally. The downwind prism was mounted on a rigid-aeroelastic setup in an open-terrain boundary layer flow to measure its acrosswind root-mean-square responses and aerodynamic damping ratios. By varying the relative location of the upwind prism and the Scruton number associated with the downwind prism, the acrosswind aeroelastic behavior of the downwind prism was analyzed and compared to that of an isolated one. Results showed that the acrosswind root-mean-square response of the downwind prism could be either suppressed or enhanced by the wake flow produced by the neighboring upwind prism. Besides the assessment of the wake effect of the downwind prism, finally, regressed relationships were presented to describe the variation of the aerodynamic damping ratio so as to predict its acrosswind fluctuating response numerically.

Quantitative and Pattern Recognition Analyses for the Quality Evaluation of Cimicifugae Rhizoma by HPLC

Fang, Zhe,Moon, Dong-Cheul,Son, Kun-Ho,Son, Jong-Keun,Min, Byung-Sun,Woo, Mi-Hee Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.1

In this study, quantitative and pattern recognition analysis for the quality evaluation of Cimicifugae Rhizoma using HPLC/UV was developed. For quantitative analysis, three major bioactive phenolic compounds were determined. The separation conditions employed for HPLC/UV were optimized using ODS $C_{18}$ column ($250{\times}4.6mm$, $5{\mu}M$) with isocratic elution of acetonitrile and water with 0.1% phosphoric acid as the mobile phase at a flow rate of 1.0 mL/min and a detection wavelength of 323 nm. These methods were fully validated with respect to the linearity, accuracy, precision, recovery, and robustness. The HPLC/UV method was applied successfully to the quantification of three major compounds in the extract of Cimicifugae Rhizoma. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of twelve reference samples corresponding to five different species of Cimicifugae Rhizoma and seventeen samples purchased from markets. The results indicate that the established HPLC/UV method is suitable for the quantitative analysis and quality control of multi-components in Cimicifugae Rhizoma.