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Chen Wenwen,Wang Genhong,Yi Meiqin,Gao Tianke,Song Chunman,Gao Qian,Xia Qingyou,Xiang Haiying 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.3
Cytokinin hormones are indispensable for plant growth and development. Cytokinin oxidase/dehydrogenase (CKX) helps regulate the dynamic balance of endogenous cytokinin levels. However, little is known about the CKX genes of Nicotiana tabacum (NtCKXs). In silico analyses were used to isolate, characterize, and elucidate the phylogenetic relationships of 15 NtCKX genes. Multi-species phylogenetic tree construction placed NtCKX1–15 on five of the eight branches of the CKX phylogenetic tree. Protein structure and network analyses revealed that NtCKX genes located on the same phylogenetic branch generally contained several conserved motifs and have highly similar structures, with structural domains related to flavin adenine dinucleotide (FAD) and cytokinin-binding found on all of the predicted NtCKX-encoded proteins. The upstream promotor region of NtCKX genes contained many abiotic stress-responsive cis-acting elements, including DRE, ERE, MBS, MYB, and MYC. Gene expression analysis revealed that each NtCKX gene responded differently to salt stress and exogenous abscisic acid (ABA) treatment. Four NtCKX genes exhibited ABA-induced expression trends with varying peak times. Under salt stress, NtCKX expression was significantly suppressed in two genes and upregulated in five others. In summary, we provided basic information regarding the CKX gene family of N. tabacum and elucidated their gene expression patterns under abiotic stresses, including ABA treatment and salt stress. The findings of this work can serve as a foundation for future study of the functions of NtCKX genes.
Wang, Chenxi,Li, Yuping,Yao, Lihua,Wu, Guangjie,Chang, Jun,Shu, Chengchuang,Chen, Meiqin 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.5
Optimization of ultrasonic extraction of Portulaca oleracea L. flavonoids (POF) was investigated using single-factor experimentation combined with response surface methodology. The optimal conditions for the highest yield (16.25 mg RE/g DW) of POF was 39.01% ethanol, $55.25^{\circ}C$ extraction temperature, 15 min extraction time, and 23.92 (v/m) liquid-to-solid ratio. The crude extract of POF was purified on the polyamide resin. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a yellow tetrazole assays of the flavonoids were evaluated, which suggested the concentrations of the flavonoids (0-1 mg/mL) and quercetin (0-1 mg/mL). Quercetin was identified in the extract by comparing relative retention time of the reference standard.
Chenxi Wang,Yuping Li,Li-Hua Yao,Guangjie Wu,Jun Chang,Chengchuang Shu,Meiqin Chen 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.5
Optimization of ultrasonic extraction of Portulacaoleracea L. flavonoids (POF) was investigated using single-factorexperimentation combined with response surface methodology. The optimal conditions for the highest yield (16.25 mg RE/g DW)of POF was 39.01% ethanol, 55.25oC extraction temperature, 15min extraction time, and 23.92 (v/m) liquid-to-solid ratio. Thecrude extract of POF was purified on the polyamide resin. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a yellowtetrazole assays of the flavonoids were evaluated, which suggestedthe concentrations of the flavonoids (0-1 mg/mL) and quercetin(0-1 mg/mL). Quercetin was identified in the extract by comparingrelative retention time of the reference standard.