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Chenxi Wang,Yuping Li,Li-Hua Yao,Guangjie Wu,Jun Chang,Chengchuang Shu,Meiqin Chen 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.5
Optimization of ultrasonic extraction of Portulacaoleracea L. flavonoids (POF) was investigated using single-factorexperimentation combined with response surface methodology. The optimal conditions for the highest yield (16.25 mg RE/g DW)of POF was 39.01% ethanol, 55.25oC extraction temperature, 15min extraction time, and 23.92 (v/m) liquid-to-solid ratio. Thecrude extract of POF was purified on the polyamide resin. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a yellowtetrazole assays of the flavonoids were evaluated, which suggestedthe concentrations of the flavonoids (0-1 mg/mL) and quercetin(0-1 mg/mL). Quercetin was identified in the extract by comparingrelative retention time of the reference standard.
Wang, Chenxi,Li, Yuping,Yao, Lihua,Wu, Guangjie,Chang, Jun,Shu, Chengchuang,Chen, Meiqin 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.5
Optimization of ultrasonic extraction of Portulaca oleracea L. flavonoids (POF) was investigated using single-factor experimentation combined with response surface methodology. The optimal conditions for the highest yield (16.25 mg RE/g DW) of POF was 39.01% ethanol, $55.25^{\circ}C$ extraction temperature, 15 min extraction time, and 23.92 (v/m) liquid-to-solid ratio. The crude extract of POF was purified on the polyamide resin. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a yellow tetrazole assays of the flavonoids were evaluated, which suggested the concentrations of the flavonoids (0-1 mg/mL) and quercetin (0-1 mg/mL). Quercetin was identified in the extract by comparing relative retention time of the reference standard.